Standard name
Human Ortholog
Description Fe(II)/2-oxoglutarate-dependent dioxygenase family member; catalyzes the repair of methyl-base lesions in both ss and dsDNA by oxidative demethylation; Poly(rA)-binding protein involved in mRNA poly(A) tail length and mRNA stability; role in translation termination efficiency; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(II) and 2-oxoglutarate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.12 0.05 0 0 0 0.05 0.05 0 0.05 0 0 0.05 0.16 0.16 0.11 0.15 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.07 0.07 0.11 0.1 0.25 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.8 0.93 0.96 0.92 0.9 0.89 0.9 0.9 0.87 0.84 0.85 0.91 0.8 0.79 0.76 0.72 0.75 0.95 0.9 0.92 0.9 0.78 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.1 0 0.05 0 0 0.11 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 2 0 0 0 1 0 0 2 1 0 2 0 0 0 0 1 0 0 4 3 0 2 3
Bud Neck 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 1 4 2 3 4 4 8 0 1 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 6 50 14 7 10 7 20 16 10 17 8 16 13 39 45 23 32 31 0 2 0 1 2 1
Endoplasmic Reticulum 2 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 5 10 0 0 1 0 2 0
Endosome 1 1 1 0 1 2 2 1 0 1 0 2 0 0 4 1 0 1 2 5 1 0 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 0 1 0 3 2 0
Mitochondria 7 6 0 0 0 18 27 24 35 32 71 56 0 0 0 6 2 6 2 1 0 0 1 5
Nucleus 386 348 264 207 233 257 336 310 275 278 244 359 216 190 227 154 150 185 382 437 278 166 260 205
Nuclear Periphery 1 5 0 3 2 0 1 1 1 0 2 4 1 1 3 2 2 1 1 1 0 0 1 1
Nucleolus 3 9 2 2 2 14 8 4 13 15 20 18 2 3 10 7 6 5 1 3 1 2 17 17
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 2 1 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 6 4 1 0 1 1 5 9 1 1 0 0 2 1 0 4 2
Vac/Vac Membrane 2 33 8 2 6 7 6 4 2 5 5 15 5 5 6 14 17 26 7 24 10 6 35 42
Unique Cell Count 401 433 284 215 253 285 376 345 307 321 289 421 237 237 287 203 207 248 402 487 303 185 332 288
Labelled Cell Count 408 456 290 221 256 307 407 368 345 355 354 481 241 246 308 212 218 269 402 487 303 185 332 288


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 11.5 9.6 9.8 8.7 6.9 6.5 6.7 5.7 6.0 5.3 5.5 12.6 10.7 10.0 13.1 12.9 12.9 10.1 10.2 9.6
Std Deviation (1e-4) 1.4 4.3 1.8 2.2 1.6 1.6 1.8 1.8 1.4 1.6 1.3 1.3 3.1 3.6 3.3 5.4 5.1 4.7 2.3 2.3 1.8
Intensity Change (Log2) – – – 0.03 -0.13 -0.48 -0.55 -0.51 -0.75 -0.68 -0.84 -0.81 0.39 0.16 0.06 0.45 0.42 0.42 0.08 0.08 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -0.5 -1.6 0.2 -0.2 -1.0 0.2 -1.3 -0.7 0.3 4.3 4.2 2.6 4.0 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 4.6 4.5 5.9 5.5 8.9 6.4 0 0 0 0 0 0
Nucleus 1.6 -0.4 -1.2 -1.6 -1.4 -1.4 -2.6 -3.2 -3.1 -0.8 -4.3 -4.8 -5.3 -6.2 -5.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.0 0 0 2.7 2.9 3.9 2.8 0 0 2.3 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.3 -0.3 -1.1 -1.5 0 -1.1 -0.9 0.5 -0.5 -0.5 -0.6 2.1 2.7 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4019 9.4859 8.2212 6.7307 6.0771 8.567 7.083 8.2557 7.0838 7.0639 5.8625 7.1759 7.1324 9.522 8.0705 8.1092 5.676 8.4925
Actin 0.0242 0 0.0004 0.0216 0.0054 0.0118 0.0095 0 0.0024 0.0092 0.0107 0.0027 0.0336 0 0.0195 0 0.0472 0.0113
Bud 0.0004 0 0.0003 0.0004 0.0006 0.0006 0.0001 0 0.0003 0.0006 0.001 0.0002 0.0009 0 0.0002 0 0.005 0.0002
Bud Neck 0.0004 0 0.0001 0.0027 0.0008 0.0009 0.0001 0 0.0046 0.0004 0.0005 0.0004 0.0008 0 0.0012 0 0.0011 0.0016
Bud Periphery 0.0004 0 0.0008 0.0005 0.002 0.0014 0.0002 0 0.0004 0.0013 0.0018 0.0002 0.0017 0 0.0004 0 0.0097 0.0003
Bud Site 0.0026 0 0.0005 0.002 0.0009 0.0008 0.0003 0.0001 0.0015 0.0012 0.0021 0.0001 0.0063 0 0.0027 0 0.0032 0.0003
Cell Periphery 0.0002 0 0.0004 0.0004 0.001 0.0003 0.0001 0 0.0001 0.0003 0.0001 0 0.0009 0 0.0002 0 0.0003 0.0001
Cytoplasm 0.0217 0 0.0004 0.0012 0.0006 0.0013 0.005 0.0003 0.0004 0.0014 0.0018 0.0033 0.0045 0 0.0004 0 0.003 0.0001
Cytoplasmic Foci 0.0262 0 0.0011 0.014 0.0044 0.0009 0.0063 0 0.0008 0.0011 0.0179 0.0006 0.0169 0 0.0076 0 0.0494 0.0014
Eisosomes 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0.0003 0.0001 0 0.0007 0 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.003 0 0.0005 0.0002 0.0026 0.0003 0.0013 0 0.0001 0.0028 0.001 0.0006 0.0071 0 0.0002 0 0.0039 0.0002
Endosome 0.0263 0 0.0007 0.0079 0.0738 0.0034 0.0067 0.0001 0.0011 0.0088 0.0164 0.0026 0.0222 0 0.0033 0 0.041 0.0044
Golgi 0.0082 0 0.0008 0.0401 0.0421 0.0019 0.0012 0 0.001 0.0018 0.0152 0.0015 0.0069 0 0.0055 0 0.0416 0.0039
Lipid Particles 0.0305 0 0.0055 0.005 0.0237 0.004 0.0068 0 0.0007 0.0025 0.022 0.0007 0.0094 0 0.0062 0 0.0149 0.0024
Mitochondria 0.0039 0.0001 0.003 0.005 0.0487 0.0089 0.0029 0.0001 0.0016 0.0434 0.041 0.001 0.0371 0.0001 0.0054 0.0001 0.0232 0.0031
None 0.0026 0 0.0002 0.0003 0.0007 0.0002 0.0073 0 0.0003 0.0011 0.0015 0.0004 0.003 0 0.0002 0 0.0008 0.0001
Nuclear Periphery 0.0047 0.001 0.0126 0.0062 0.0174 0.0018 0.0148 0.0013 0.0022 0.0269 0.0158 0.0044 0.0152 0.0006 0.001 0.0003 0.0049 0.0009
Nucleolus 0.032 0.0062 0.0221 0.0886 0.111 0.0492 0.0149 0.0071 0.0065 0.0182 0.0385 0.0316 0.0198 0.0056 0.0058 0.01 0.056 0.0228
Nucleus 0.7776 0.9926 0.9392 0.7789 0.6294 0.9032 0.8992 0.9889 0.9668 0.8684 0.7823 0.9453 0.792 0.9935 0.9211 0.9894 0.6367 0.9402
Peroxisomes 0.013 0 0.0045 0.0162 0.0014 0.0009 0.0022 0 0.0006 0.0008 0.0149 0.0001 0.0061 0 0.0092 0 0.0476 0.0011
Punctate Nuclear 0.0184 0 0.0015 0.0052 0.001 0.0013 0.0166 0.0006 0.0082 0.0023 0.013 0.0022 0.0061 0 0.0006 0.0001 0.0039 0.0043
Vacuole 0.0019 0 0.0028 0.0026 0.0086 0.0051 0.0019 0.0011 0.0004 0.003 0.001 0.0013 0.0041 0.0001 0.0038 0 0.0035 0.0007
Vacuole Periphery 0.0016 0 0.0027 0.0008 0.0238 0.0019 0.0023 0.0004 0.0002 0.0042 0.0014 0.0005 0.0047 0.0002 0.0053 0 0.0028 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 73.8454 82.0706 100.239 67.6756 79.1088 59.2716 78.2139 65.9212 58.1071 71.3651
Translational Efficiency 1.372 1.4187 1.0251 1.3245 1.0548 1.1687 1.2073 1.1928 1.1871 1.0308

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Fe(II)/2-oxoglutarate-dependent dioxygenase family member; catalyzes the repair of methyl-base lesions in both ss and dsDNA by oxidative demethylation; Poly(rA)-binding protein involved in mRNA poly(A) tail length and mRNA stability; role in translation termination efficiency; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(II) and 2-oxoglutarate
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tpa1

Tpa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available