Standard name
Description Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0 0.05 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.97 0.99 0.95 0.95 0.82 0.77 0.81 0.75 0.75 0.76 0.99 0.98 0.96 0.98 0.99 0.99 0.99 0.95 0.92 0.93 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.14 0.17 0.09 0.15 0.15 0.05 0 0 0 0.06 0.08 0.07 0 0 0.05 0 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 8 7 12 16 4 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 2 2 0 0 2 0 1 0 1 0 0 0 2 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 2 5 5 20 10 12 19 5 6 0 0 2 3 6 3 0 0 0 0 0 0
Cytoplasm 128 193 299 182 294 234 328 303 210 241 76 87 94 197 238 717 384 303 120 184 276 82 204 140
Endoplasmic Reticulum 0 3 9 0 11 9 55 69 22 50 15 6 1 4 11 45 31 21 0 2 16 3 9 10
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 1 0 0 3 7 6 7 3 2 0 0 0 3 1 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 2 3 13 19 5 8 0 0 0 5 1 0 0 0 1 0 1 1
Nucleus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 0 1 6 2 0 2 2 0 0 0 2 1 0 0 1 1 0 0 2
Unique Cell Count 128 196 308 184 309 246 402 395 258 323 102 115 95 200 248 728 388 306 122 195 302 89 220 158
Labelled Cell Count 128 196 311 186 314 249 421 409 280 352 111 126 95 202 251 776 424 327 122 195 302 89 220 158


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 89.2 101.5 86.0 85.3 72.5 64.2 51.4 43.8 41.6 38.4 33.8 36.7 91.1 88.6 86.2 115.0 133.8 142.1 80.3 84.3 86.6
Std Deviation (1e-4) 19.5 24.0 20.6 23.6 20.5 15.9 13.2 12.9 13.5 11.9 10.8 13.4 16.6 18.5 17.1 28.6 35.8 41.1 23.6 17.7 19.1
Intensity Change (Log2) -0.01 -0.25 -0.42 -0.74 -0.97 -1.05 -1.16 -1.35 -1.23 0.08 0.04 0.0 0.42 0.64 0.73 -0.1 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.8 4.0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.3 1.9 3.1 3.7 0 0 0 0 0 0 0 0
Cytoplasm 1.3 -1.2 -1.2 -6.4 -7.6 -6.2 -8.0 -7.1 -6.9 1.0 1.0 -0.7 1.5 1.8 1.7
Endoplasmic Reticulum 0 0.4 0.5 5.0 6.1 2.9 5.4 4.4 0 0 -0.6 1.0 2.2 2.9 2.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.0 4.3 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hom3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available