Standard name
Human Ortholog
Description Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.07 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.84 0.87 0.86 0.81 0.62 0.66 0.52 0.43 0.58 0.51 0.61 0.66 0.73 0.67 0.64 0.55 0.35 0.25 0.5 0.43 0.68 0.57 0.54 0.46
Cytoplasm 0.21 0.37 0.11 0.12 0.14 0.14 0.15 0.18 0.25 0.22 0.31 0.19 0 0 0.06 0.51 0.74 0.8 0.17 0.34 0 0.09 0 0
Endoplasmic Reticulum 0.08 0 0.06 0.08 0.3 0.25 0.36 0.39 0.25 0.33 0.22 0.2 0.26 0.35 0.29 0 0.07 0 0.3 0.15 0.16 0.18 0.26 0.34
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.11 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 15 7 8 9 12 7 21 23 21 40 0 0 0 0 2 7 4 8 9
Bud 0 1 2 2 0 2 9 10 9 5 11 17 15 15 14 0 1 0 0 1 4 2 7 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 53 59 168 125 121 152 149 152 188 159 151 263 535 535 511 29 51 10 56 57 235 171 316 320
Cytoplasm 13 25 21 18 28 33 42 65 81 69 76 78 20 20 51 27 106 32 18 46 8 26 24 17
Endoplasmic Reticulum 5 1 11 12 59 57 102 139 80 101 55 81 193 280 227 0 10 0 33 19 54 52 153 238
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 2 0 0 1 1 2 1 0 2 21 12 41 0 0 0 0 0 0 0 3 0
Mitochondria 0 0 0 0 0 0 0 0 3 1 1 0 0 0 0 0 3 1 1 2 30 34 67 95
Nucleus 0 0 0 1 1 0 4 6 3 5 2 9 1 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3 2 3 3 3 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 2 6 6 5 5 3 11 3 3 5 0 0 0 0 1 1 1 2 2
Unique Cell Count 63 68 195 154 196 229 286 354 325 309 249 401 732 798 795 53 144 40 113 134 346 298 586 703
Labelled Cell Count 71 86 204 162 209 261 320 390 382 361 310 485 811 887 894 57 171 43 113 134 346 298 586 703


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 138.8 122.7 118.1 119.6 120.3 96.2 87.7 83.7 80.9 76.8 74.3 75.6 80.2 80.2 58.8 85.5 94.5 119.1 125.5 116.7 118.5
Std Deviation (1e-4) 23.6 24.8 29.4 34.8 33.3 32.9 30.8 28.2 28.0 25.2 26.4 22.5 16.3 16.3 14.6 23.3 30.1 39.7 29.0 30.7 27.9
Intensity Change (Log2) 0.02 0.03 -0.3 -0.43 -0.5 -0.55 -0.62 -0.67 -0.64 -0.56 -0.56 -1.01 -0.47 -0.32 0.01 0.09 -0.02 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 2.9 0 0 0 1.9 0 2.5 1.6 0 2.5 0 0 0
Bud 0 0 0 0 0 0 0 2.1 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -5.5 -4.7 -7.7 -9.8 -6.7 -7.9 -5.9 -5.3 -3.8 -5.3 -5.9 -5.0 -9.7 -8.2
Cytoplasm 0.3 1.1 1.1 1.2 2.3 3.9 3.3 5.0 2.7 -4.9 -5.2 -2.1 6.6 11.8 9.5
Endoplasmic Reticulum 0.8 6.3 5.4 7.6 8.5 5.5 7.1 4.8 4.6 6.2 8.1 6.7 0 0.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 80.5676 131.085 118.14 109.8896 122.3924 117.49 93.0527 139.9834 128.8896 123.0101 90.6754 130.7585 94.791 146.5711 133.5515 113.6739 87.7028 132.6287
Actin 0.0305 0 0.0007 0.0103 0 0.0015 0.0145 0.0003 0.0058 0.0056 0.0002 0 0.0222 0 0.0001 0.0009 0.0006 0.0001
Bud 0.0014 0 0.0001 0.0002 0 0.0002 0.0007 0.0001 0.0001 0.0001 0.001 0.0001 0.0009 0.0001 0.0003 0.0002 0.0006 0.0001
Bud Neck 0.0016 0.0001 0.0005 0.0009 0.0001 0.0003 0.0007 0.0005 0.0018 0.0011 0.0009 0.0004 0.006 0.0003 0.0013 0.001 0.0007 0.0011
Bud Periphery 0.0021 0.0002 0.0005 0.0013 0.0003 0.0012 0.003 0.0005 0.0011 0.0009 0.0027 0.0011 0.0029 0.0004 0.0017 0.0025 0.0018 0.0013
Bud Site 0.0127 0.0004 0.0004 0.0005 0 0.0001 0.0097 0.0043 0.0024 0.0002 0.0033 0 0.0053 0.0078 0.003 0.0002 0.0038 0.0003
Cell Periphery 0.5371 0.6531 0.6674 0.6835 0.7291 0.6354 0.692 0.7614 0.7513 0.7438 0.5934 0.7083 0.7119 0.8006 0.8101 0.7336 0.6253 0.7069
Cytoplasm 0.0086 0.0037 0.0025 0.0042 0.0009 0.0007 0.0012 0.0008 0.0018 0.0002 0.0011 0.0001 0.0012 0.0009 0.0024 0.0046 0.0004 0.0008
Cytoplasmic Foci 0.0185 0 0.0109 0.0024 0 0.0003 0.0123 0.0001 0.0015 0.0119 0.0926 0 0.0079 0 0.0003 0.0004 0.0008 0.023
Eisosomes 0.0032 0.0005 0.0011 0.001 0.0004 0.0005 0.0095 0.006 0.0125 0.0026 0.0008 0.0002 0.0052 0.0093 0.0049 0.0027 0.0307 0.0082
Endoplasmic Reticulum 0.0059 0.0031 0.0063 0.0133 0.0042 0.0048 0.0037 0.0017 0.0036 0.0041 0.0053 0.0011 0.003 0.001 0.0019 0.0054 0.0023 0.0013
Endosome 0.0067 0 0.0012 0.0036 0 0.0073 0.0064 0.0017 0.0016 0.0005 0.0003 0 0.0005 0 0.0002 0.0008 0.0001 0.0001
Golgi 0.0045 0 0.0003 0.0011 0 0.0014 0.0084 0.0003 0.0006 0.0018 0.0002 0 0.001 0 0 0.0001 0.0001 0.0001
Lipid Particles 0.0105 0.0048 0.0099 0.0038 0.0021 0.0053 0.0064 0.0045 0.0076 0.0037 0.0163 0.0008 0.0039 0.0017 0.0012 0.0014 0.0028 0.005
Mitochondria 0.0058 0 0.0006 0.0008 0 0.0017 0.0107 0.0001 0.0002 0.0003 0.0084 0 0.0057 0.0001 0.0016 0.0003 0.156 0.0075
None 0.0178 0 0.0009 0.0018 0 0.0005 0.0032 0 0.0009 0.0001 0.0054 0 0.0021 0 0.0038 0.0443 0.0081 0.0077
Nuclear Periphery 0.0092 0.0144 0.0207 0.0389 0.0088 0.0236 0.016 0.0132 0.0206 0.0173 0.0164 0.0059 0.0148 0.0125 0.013 0.0157 0.0107 0.0073
Nucleolus 0.0187 0.0004 0.0008 0.0008 0.0002 0.0011 0.0081 0.0008 0.0012 0.0012 0.0905 0.0007 0.0086 0.001 0.0018 0.0031 0.0187 0.0144
Nucleus 0.0093 0.0003 0.0045 0.0023 0.0003 0.0013 0.0011 0.0007 0.0009 0.0008 0.0144 0.0006 0.0019 0.0009 0.0014 0.0071 0.003 0.0017
Peroxisomes 0.0113 0 0.0005 0.0002 0 0.0002 0.0025 0 0.0004 0.0268 0.0022 0 0.0007 0 0.0001 0.0001 0.003 0.0009
Punctate Nuclear 0.0115 0 0.0014 0.0039 0 0.0009 0.0005 0 0.0001 0.0003 0.0017 0 0.0015 0 0 0.0085 0 0.0005
Vacuole 0.2695 0.318 0.2676 0.2217 0.2526 0.3086 0.1863 0.2014 0.1818 0.1739 0.1385 0.2771 0.1902 0.1623 0.1477 0.1628 0.1262 0.2084
Vacuole Periphery 0.0036 0.001 0.0014 0.0033 0.0012 0.003 0.003 0.0015 0.0022 0.0028 0.0046 0.0035 0.0028 0.0008 0.0032 0.0042 0.0041 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 139.8197 107.1345 139.8076 173.7889 147.7942 99.1275 122.7978 116.8097 150.3507 165.3681
Translational Efficiency 1.3335 1.339 1.2966 1.1141 1.1984 1.4782 1.1498 1.0293 1.019 1.0281

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
100 234 902 418 1674 588 1054 615 1774 822 1956 1033

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1711.57 8335.32 1958.86 1657.25 7061.02 7253.51 2607.12 1687.87 6759.47 7561.47 2308.18 1675.48
Standard Deviation 376.18 1635.42 406.11 324.55 2036.57 1641.67 664.98 458.45 2333.23 1711.01 647.11 409.85
Intensity Change Log 2 2.283917 0.194694 -0.046529 0.038803 -1.437420 -2.064673 0.829438 -0.942078 -1.390945

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000216 0.000273 0.001190 0.001789 0.000338 0.000296 0.012853 0.001175 0.000331 0.000289 0.007475 0.001423
Bud Neck 0.004797 0.000710 0.005901 0.005476 0.001169 0.000885 0.007679 0.005347 0.001373 0.000835 0.006859 0.005399
Bud Site 0.019870 0.003577 0.006660 0.016151 0.005502 0.001522 0.019934 0.023376 0.006312 0.002107 0.013812 0.020452
Cell Periphery 0.009347 0.536844* 0.002249 0.007953 0.459991* 0.602810* 0.010663 0.004330 0.434588* 0.584031* 0.006783 0.005796
Cytoplasm 0.015899 0.000171 0.000785 0.002894 0.020591 0.000319 0.002002 0.006624 0.020327 0.000277 0.001441 0.005115
Cytoplasmic Foci 0.011290 0.000017 0.013978 0.011917 0.000136 0.000073 0.006969 0.033575 0.000764 0.000057 0.010201 0.024811
Eisosomes 0.000035 0.006039 0.000123 0.000047 0.001029 0.003124 0.000701 0.000037 0.000973 0.003954 0.000435 0.000041
Endoplasmic Reticulum 0.017478 0.001308 0.001310 0.038350 0.001349 0.002591 0.016330 0.008554 0.002259 0.002226 0.009404 0.020611
Endosome 0.027159 0.000129 0.083338 0.089083 0.000205 0.000254 0.180773 0.158240 0.001724 0.000218 0.135841 0.130256
Golgi 0.028042 0.000384 0.077728 0.049370 0.001135 0.001616 0.123820* 0.094676 0.002652 0.001265 0.102565 0.076343
Lipid Particles 0.007590 0.000978 0.090188 0.045138 0.005010 0.001031 0.238405* 0.055696 0.005155 0.001016 0.170056* 0.051424
Mitochondria 0.000170 0.000051 0.003935 0.000567 0.000150 0.000642 0.004847 0.004943 0.000151 0.000473 0.004426 0.003172
Mitotic Spindle 0.001002 0.000579 0.000276 0.001455 0.000415 0.000578 0.000654 0.010234 0.000448 0.000579 0.000480 0.006682
None 0.000027 0.000004 0.000125 0.000041 0.000111 0.000008 0.000198 0.000221 0.000106 0.000007 0.000165 0.000148
Nuclear Periphery 0.000893 0.003569 0.002997 0.001291 0.006473 0.006180 0.000899 0.000848 0.006159 0.005437 0.001866 0.001027
Nuclear Periphery Foci 0.004656 0.000202 0.011334 0.018130 0.000799 0.000213 0.003539 0.010348 0.001016 0.000210 0.007134 0.013497
Nucleolus 0.001278 0.000047 0.001126 0.000131 0.000103 0.000065 0.000202 0.001226 0.000169 0.000060 0.000629 0.000783
Nucleus 0.001305 0.000060 0.000211 0.000543 0.000359 0.000086 0.000352 0.000907 0.000413 0.000079 0.000287 0.000760
Peroxisomes 0.000252 0.000028 0.000456 0.000170 0.000117 0.000035 0.000591 0.000693 0.000125 0.000033 0.000529 0.000481
Vacuole 0.786356* 0.442470* 0.576005* 0.671123* 0.490311* 0.374441* 0.320680* 0.525892* 0.506999* 0.393807* 0.438422* 0.584659*
Vacuole Periphery 0.062336 0.002557 0.120085* 0.038381 0.004707 0.003231 0.047907 0.053058 0.007956 0.003039 0.081191 0.047119

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.11 -2.58 -1.94 -1.85 -0.68 0.40 -6.60 -1.77 -1.88 5.97 0.45 -6.82 -2.50 -2.63 5.35
Bud Neck 2.96 -0.63 -0.40 -5.61 0.32 0.61 -10.70 -6.47 -6.81 2.85 1.42 -10.32 -7.28 -8.64 2.13
Bud Site 2.34 2.10 0.80 -2.94 -3.12 3.28 -5.57 -5.47 -6.79 -0.56 2.95 -4.42 -5.84 -7.57 -2.23
Cell Periphery -32.83* 2.71 0.40 33.24* -6.67 -11.31 65.88* 67.29* 55.41* 4.19 -13.32 62.63 62.81 64.20* 1.13
Cytoplasm 3.02 2.92 2.59 -4.05 -2.78 8.75 7.32 4.70 -5.42 -3.25 9.19 8.30 6.13 -6.46 -4.17
Cytoplasmic Foci 3.05 -0.13 0.33 -5.13 0.80 1.33 -9.20 -10.20 -10.22 -7.85 3.19 -10.67 -10.85 -11.30 -6.19
Eisosomes -3.26 -4.66 -0.89 3.25 4.64 -2.24 1.18 3.75 3.44 7.19 -3.44 2.12 3.73 4.72 7.77
Endoplasmic Reticulum 2.91 2.91 -2.70 -6.19 -6.20 -1.24 -8.14 -4.18 -2.99 3.08 -0.01 -6.78 -6.85 -6.66 -3.96
Endosome 7.45 -9.68 -8.07 -13.30 -0.66 -0.53 -24.00 -21.36 -21.35 2.15 5.72 -28.65 -24.35 -24.67 0.84
Golgi 3.88 -6.18 -2.42 -8.17 3.86 -0.45 -22.44 -14.49 -14.22 3.62 1.54 -27.18 -15.97 -16.12 4.68
Lipid Particles 2.22 -11.73 -5.26 -6.74 4.89 4.57 -25.65 -8.89 -9.67 17.11 5.11 -27.49 -10.64 -11.76 16.17
Mitochondria 1.89 -5.97 -1.35 -1.96 4.99 -0.92 -5.73 -2.23 -1.94 0.04 -0.82 -8.09 -2.35 -2.01 1.01
Mitotic Spindle 0.77 0.99 0.08 -1.77 -2.33 -3.95 -1.01 -3.83 -3.78 -3.63 -1.16 -0.46 -4.00 -3.95 -3.90
None 3.08 -5.50 -0.97 -3.15 4.08 4.43 -1.80 -1.15 -2.17 -0.04 4.55 -1.86 -0.77 -2.41 0.35
Nuclear Periphery -4.82 -3.54 -0.84 4.27 2.97 0.40 17.65 16.77 8.30 0.27 1.30 11.57 15.48 8.97 3.08
Nuclear Periphery Foci 3.66 -2.84 -3.60 -5.24 -1.89 6.78 -10.53 -7.36 -7.80 -5.24 7.42 -7.51 -7.86 -8.39 -3.76
Nucleolus 1.21 0.11 1.13 -5.01 4.41 3.50 -5.66 -1.66 -1.71 -1.50 1.91 -3.93 -1.51 -1.79 -0.35
Nucleus 2.10 1.90 1.24 -1.96 -1.43 5.97 -0.64 -1.67 -2.47 -1.14 5.90 0.57 -1.57 -3.10 -1.75
Peroxisomes 2.00 -1.20 0.80 -3.48 2.74 4.86 -2.45 -5.50 -6.32 -0.53 5.51 -3.55 -5.38 -6.92 0.32
Vacuole 12.03 8.30 3.91 -10.13 -5.29 9.09 14.39 -2.47 -8.99 -12.67 9.98 6.84 -6.43 -13.99 -11.67
Vacuole Periphery 5.74 -5.59 2.10 -13.63 16.87 4.14 -19.73 -14.21 -14.69 -1.10 6.70 -29.70 -16.41 -19.27 10.63