Standard name
Description Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.07 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.84 0.87 0.86 0.81 0.62 0.66 0.52 0.43 0.58 0.51 0.61 0.66 0.73 0.67 0.64 0.55 0.35 0.25 0.5 0.43 0.68 0.57 0.54 0.46
Cytoplasm 0.21 0.37 0.11 0.12 0.14 0.14 0.15 0.18 0.25 0.22 0.31 0.19 0 0 0.06 0.51 0.74 0.8 0.17 0.34 0 0.09 0 0
Endoplasmic Reticulum 0.08 0 0.06 0.08 0.3 0.25 0.36 0.39 0.25 0.33 0.22 0.2 0.26 0.35 0.29 0 0.07 0 0.3 0.15 0.16 0.18 0.26 0.34
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.11 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 15 7 8 9 12 7 21 23 21 40 0 0 0 0 2 7 4 8 9
Bud 0 1 2 2 0 2 9 10 9 5 11 17 15 15 14 0 1 0 0 1 4 2 7 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 53 59 168 125 121 152 149 152 188 159 151 263 535 535 511 29 51 10 56 57 235 171 316 320
Cytoplasm 13 25 21 18 28 33 42 65 81 69 76 78 20 20 51 27 106 32 18 46 8 26 24 17
Endoplasmic Reticulum 5 1 11 12 59 57 102 139 80 101 55 81 193 280 227 0 10 0 33 19 54 52 153 238
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 2 0 0 1 1 2 1 0 2 21 12 41 0 0 0 0 0 0 0 3 0
Mitochondria 0 0 0 0 0 0 0 0 3 1 1 0 0 0 0 0 3 1 1 2 30 34 67 95
Nucleus 0 0 0 1 1 0 4 6 3 5 2 9 1 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3 2 3 3 3 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 2 6 6 5 5 3 11 3 3 5 0 0 0 0 1 1 1 2 2
Unique Cell Count 63 68 195 154 196 229 286 354 325 309 249 401 732 798 795 53 144 40 113 134 346 298 586 703
Labelled Cell Count 71 86 204 162 209 261 320 390 382 361 310 485 811 887 894 57 171 43 113 134 346 298 586 703


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 138.8 122.7 118.1 119.6 120.3 96.2 87.7 83.7 80.9 76.8 74.3 75.6 80.2 80.2 58.8 85.5 94.5 119.1 125.5 116.7 118.5
Std Deviation (1e-4) 23.6 24.8 29.4 34.8 33.3 32.9 30.8 28.2 28.0 25.2 26.4 22.5 16.3 16.3 14.6 23.3 30.1 39.7 29.0 30.7 27.9
Intensity Change (Log2) – – – 0.02 0.03 -0.3 -0.43 -0.5 -0.55 -0.62 -0.67 -0.64 -0.56 -0.56 -1.01 -0.47 -0.32 0.01 0.09 -0.02 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 2.9 0 0 0 1.9 0 2.5 1.6 0 2.5 0 0 0
Bud 0 0 0 0 0 0 0 2.1 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -5.5 -4.7 -7.7 -9.8 -6.7 -7.9 -5.9 -5.3 -3.8 -5.3 -5.9 -5.0 -9.7 -8.2
Cytoplasm 0.3 1.1 1.1 1.2 2.3 3.9 3.3 5.0 2.7 -4.9 -5.2 -2.1 6.6 11.8 9.5
Endoplasmic Reticulum 0.8 6.3 5.4 7.6 8.5 5.5 7.1 4.8 4.6 6.2 8.1 6.7 0 0.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0