Standard name
Human Ortholog
Description Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.95 0.95 0.89 0.88 0.86 0.8 0.77 0.72 0.72 0.66 0.75 0.94 0.88 0.86 0.96 0.92 0.9 0.92 0.92 0.92 0.89 0.84 0.84
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.14 0.12 0.18 0.16 0.23 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0.07 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0.07 0.16 0.13 0.09 0.14 0.16 0.15 0.19 0.21 0.13 0 0.16 0.16 0 0.06 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 5
Bud 0 1 0 4 1 2 9 7 6 6 10 11 0 1 0 0 0 0 0 0 0 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 12
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 2 0 0 1 4 2 6 2 4 5 1 2 0 7 6 2 0 0 0 0 0 0
Cytoplasm 208 76 296 230 314 397 500 396 338 414 319 352 351 373 388 235 262 206 247 103 289 196 393 394
Endoplasmic Reticulum 0 4 3 1 1 6 2 1 5 3 1 7 4 3 2 8 9 13 3 0 1 1 1 1
Endosome 3 0 0 3 6 3 2 9 0 4 2 2 0 2 11 1 1 0 2 0 1 0 2 4
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 2 2 3 0 1 0 0 0
Mitochondria 6 0 1 3 9 37 84 64 83 94 110 80 0 0 0 0 3 4 0 0 2 1 1 4
Nucleus 1 1 3 1 4 13 14 12 20 13 9 11 18 20 23 7 5 2 3 3 3 6 31 21
Nuclear Periphery 1 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 2 0 0 0 0 0 2 1
Nucleolus 0 0 0 1 1 0 0 0 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3
Vac/Vac Membrane 19 2 23 41 46 41 84 81 69 109 101 60 16 66 71 7 16 29 2 2 6 6 19 11
Unique Cell Count 228 80 312 259 356 459 622 517 468 579 481 471 372 425 450 246 286 229 269 113 314 222 470 471
Labelled Cell Count 239 84 329 285 383 501 700 573 530 645 558 528 390 467 495 266 307 259 269 113 314 222 470 471


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.1 7.6 6.9 7.0 6.4 6.4 6.4 6.2 6.1 5.9 5.9 7.8 8.0 7.4 10.6 10.8 10.5 7.4 7.5 7.8
Std Deviation (1e-4) 0.8 1.0 1.0 1.1 1.4 1.2 1.4 1.3 1.4 1.2 1.4 1.2 0.9 1.0 0.9 1.4 1.6 1.7 1.0 1.1 1.2
Intensity Change (Log2) -0.13 -0.11 -0.25 -0.25 -0.25 -0.28 -0.3 -0.37 -0.35 0.04 0.07 -0.03 0.48 0.52 0.48 -0.02 -0.0 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -3.1 -3.8 -5.9 -6.9 -7.9 -8.3 -9.4 -7.3 -0.3 -3.3 -3.9 0.4 -1.6 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.9 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 6.6 6.3 7.7 7.3 8.9 7.5 0 0 0 0 0 0
Nucleus 0 0 1.8 1.4 1.4 2.7 1.4 0 1.4 2.9 2.9 3.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.2 2.4 0.8 2.8 3.5 3.1 4.6 5.2 2.4 -1.7 3.4 3.5 -2.4 -0.9 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4844 4.4128 4.3319 3.6821 3.5081 4.1909 1.8825 2.4943 2.3532 1.781 1.3838 1.9674 2.107 2.8834 2.2887 2.3095 2.1439 2.2642
Actin 0.0467 0.0006 0.0067 0.0005 0.0005 0.0006 0.0337 0.0016 0.005 0.0009 0.0007 0.0156 0.0121 0.0011 0.0028 0.0018 0.0172 0.0015
Bud 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.001 0.0002 0.0003 0.0003 0.0001 0.0014 0.0013 0.003 0.0028 0.0014 0.0094 0.0065
Bud Neck 0.0007 0.0002 0.0004 0.0002 0.001 0.0034 0.0086 0.0003 0.0009 0.0012 0.0005 0.0045 0.0018 0.0003 0.0018 0.0017 0.0016 0.0115
Bud Periphery 0.0015 0 0.0001 0 0 0 0.0008 0.0001 0.0002 0.0001 0.0001 0.0024 0.0009 0.0005 0.0009 0.0025 0.004 0.0013
Bud Site 0.0027 0.0009 0.0042 0.0001 0.0001 0.0001 0.0068 0.0015 0.0015 0.0003 0.0001 0.0011 0.0141 0.0108 0.012 0.0063 0.0013 0.0003
Cell Periphery 0.0006 0.0002 0.0001 0.0001 0 0 0.0002 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001
Cytoplasm 0.5518 0.9156 0.8583 0.8755 0.8964 0.8625 0.7901 0.9391 0.881 0.9029 0.8626 0.9004 0.7083 0.9189 0.8513 0.8498 0.7077 0.8561
Cytoplasmic Foci 0.0158 0.0031 0.0084 0.0037 0.0055 0.0044 0.025 0.0031 0.0118 0.0167 0.0035 0.0055 0.0439 0.004 0.0178 0.0297 0.015 0.0147
Eisosomes 0.0005 0 0 0 0 0 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0191 0.0031 0.0034 0.0041 0.0023 0.0028 0.0094 0.0037 0.0031 0.0028 0.0117 0.0035 0.0071 0.0024 0.0025 0.0018 0.0024 0.0027
Endosome 0.0897 0.0007 0.0021 0.0015 0.0014 0.0012 0.0276 0.0027 0.019 0.0358 0.0635 0.0138 0.0389 0.0007 0.0123 0.0202 0.027 0.0055
Golgi 0.019 0.0001 0.0012 0.0002 0.0001 0.0001 0.0086 0.0008 0.0064 0.0081 0.0022 0.0056 0.0072 0.0001 0.0038 0.0096 0.031 0.0008
Lipid Particles 0.0179 0.0001 0.0006 0.0001 0 0 0.0108 0.0009 0.0085 0.0004 0.0006 0.0037 0.0185 0 0.0053 0.0074 0.0172 0.0021
Mitochondria 0.028 0.0001 0.0004 0.0001 0.0001 0.0001 0.0031 0.0003 0.0082 0.0022 0.0022 0.0066 0.0028 0.0001 0.0019 0.0035 0.102 0.0019
None 0.1274 0.0649 0.0879 0.1078 0.083 0.1058 0.0137 0.0336 0.0104 0.01 0.006 0.0028 0.071 0.0299 0.0309 0.0234 0.0193 0.0279
Nuclear Periphery 0.0179 0.0011 0.0014 0.001 0.0008 0.001 0.0136 0.0024 0.0051 0.0025 0.0287 0.0033 0.0154 0.0017 0.003 0.0022 0.0031 0.0091
Nucleolus 0.0006 0 0 0 0 0 0.0005 0 0.0035 0.0002 0.0001 0.0011 0.0007 0.0001 0.0006 0.0006 0.0003 0.0022
Nucleus 0.0358 0.008 0.0117 0.0041 0.0065 0.0167 0.0262 0.0078 0.026 0.0094 0.008 0.0222 0.025 0.0216 0.0372 0.0223 0.0234 0.041
Peroxisomes 0.0062 0.0001 0.0111 0.0001 0.0001 0 0.0109 0.0002 0.0038 0.0012 0.0001 0.001 0.0128 0.0001 0.0089 0.0084 0.0105 0.0034
Punctate Nuclear 0.005 0.0007 0.001 0.0005 0.0014 0.0008 0.0048 0.0009 0.0022 0.0032 0.0008 0.0012 0.0132 0.0041 0.0025 0.0037 0.0021 0.0082
Vacuole 0.0068 0.0004 0.0005 0.0003 0.0004 0.0004 0.0028 0.0007 0.0019 0.0013 0.0037 0.003 0.0033 0.0005 0.0013 0.0028 0.0026 0.0018
Vacuole Periphery 0.0056 0 0.0001 0.0001 0.0001 0.0001 0.0016 0.0001 0.0009 0.0005 0.0048 0.0009 0.0015 0.0001 0.0003 0.0006 0.0025 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1155 4.6859 4.0566 12.8721 16.9906 5.2389 11.8447 13.8569 10.9889 13.5625
Translational Efficiency 2.1752 2.9221 3.8414 0.9961 0.9675 3.8731 1.5229 1.128 1.9559 1.7682

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency