Standard name
Human Ortholog
Description Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0.05 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0.06 – 0 0 0.05 0.06 – – – 0.1 0.1 0.1 0 0 0 0 0 0
Cytoplasm 0.99 0.96 1.0 0.91 0.95 0.89 0.78 – 0.73 0.72 0.68 0.73 – – – 0.9 0.94 0.93 0.92 0.95 0.79 0.76 0.82 0.75
Endoplasmic Reticulum 0 0 0 0.06 0 0.1 0.17 – 0.21 0.16 0.13 0.15 – – – 0 0 0 0 0 0.07 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.09 0.06 0.23 0.09 – – – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.07 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 6 – 6 7 6 7 – – – 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 1 0 – – – 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 2 5 1 4 7 6 14 – 5 4 7 12 – – – 44 32 36 0 0 0 0 0 0
Cytoplasm 172 137 21 102 176 164 170 – 131 110 97 143 – – – 385 291 347 160 134 42 6 41 39
Endoplasmic Reticulum 2 6 0 7 8 18 36 – 37 25 19 30 – – – 15 7 16 5 4 4 0 0 1
Endosome 0 0 0 0 0 0 0 – 0 0 1 0 – – – 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 2 – 0 0 1 5 – – – 4 1 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 3 – 17 9 33 17 – – – 1 0 0 1 0 1 0 0 1
Nucleus 0 0 0 3 2 2 5 – 1 1 0 0 – – – 7 6 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 – 1 1 1 0 – – – 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 – – – 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 0 1 – 1 4 1 5 – – – 18 4 12 1 0 3 0 3 2
Unique Cell Count 174 143 21 112 186 185 218 179 152 143 195 428 311 375 175 142 54 9 50 52
Labelled Cell Count 177 148 22 117 194 191 238 199 161 167 220 474 342 415 175 142 54 9 50 52


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.0 18.1 11.9 13.9 15.1 13.8 13.3 – 11.7 12.4 11.2 12.2 – – – 20.5 19.5 19.2 – – 11.7
Std Deviation (1e-4) 2.3 2.7 2.1 2.0 1.9 2.2 1.9 – 2.6 2.7 2.1 2.8 – – – 3.8 3.1 3.0 – – 3.4
Intensity Change (Log2) – – – 0.23 0.35 0.22 0.16 – -0.02 0.06 -0.09 0.04 – – – 0.78 0.71 0.69 – – -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm -1.4 -1.1 -1.6 -2.4 – -2.7 -2.8 -3.1 -2.7 – – – -1.5 -1.2 -1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6305 11.0687 10.1501 9.0568 8.8444 9.749 7.413 9.8615 9.024 8.7559 9.479 9.4276 7.363 10.0593 9.0367 8.3555 7.7919 9.2933
Actin 0.0018 0.0009 0.0019 0.0023 0.0124 0.0012 0.0223 0.0008 0.0116 0.0027 0.0023 0.0005 0.0093 0.0007 0.0129 0.0057 0.0092 0.0179
Bud 0.0012 0.0006 0.0013 0.0023 0.0007 0.0024 0.0008 0.0005 0.0008 0.0007 0.0004 0.0004 0.0004 0.0003 0.0042 0.0197 0.0002 0.0014
Bud Neck 0.0006 0.0004 0.0006 0.0013 0.0012 0.0016 0.0019 0.0002 0.0007 0.0333 0.0006 0.0009 0.0006 0.0002 0.0004 0.0007 0.0004 0.0011
Bud Periphery 0.0008 0.0003 0.0005 0.0019 0.0004 0.0011 0.0009 0.0001 0.0005 0.0005 0.0002 0.0003 0.0003 0.0001 0.0015 0.0082 0.0001 0.0006
Bud Site 0.0011 0.0034 0.0034 0.0021 0.0032 0.0005 0.0111 0.0017 0.0026 0.001 0.0003 0.0001 0.001 0.0003 0.0013 0.0038 0.0002 0.0001
Cell Periphery 0.0005 0.0002 0.0003 0.0002 0.0001 0.0004 0.0007 0.0001 0.0004 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0007 0 0.0001
Cytoplasm 0.7384 0.9333 0.8419 0.7773 0.8061 0.8053 0.6486 0.9066 0.7873 0.7449 0.7449 0.813 0.724 0.9443 0.8203 0.6881 0.7025 0.7766
Cytoplasmic Foci 0.0281 0.0112 0.0198 0.0214 0.0259 0.0239 0.0259 0.0178 0.0277 0.0156 0.036 0.0205 0.0266 0.0088 0.0266 0.0213 0.0296 0.0279
Eisosomes 0 0 0 0 0.0001 0 0.0006 0 0.0001 0 0 0 0.0001 0 0 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0603 0.0158 0.0263 0.0451 0.0079 0.0364 0.1099 0.0249 0.0309 0.0384 0.0333 0.0725 0.0934 0.0124 0.0429 0.0424 0.0096 0.0494
Endosome 0.0917 0.0209 0.0696 0.0833 0.0816 0.0703 0.0897 0.0348 0.0654 0.0976 0.1448 0.0681 0.0603 0.0234 0.0549 0.1075 0.1327 0.0943
Golgi 0.0213 0.0028 0.011 0.0099 0.0277 0.017 0.0149 0.0047 0.0168 0.0048 0.0169 0.0072 0.0153 0.0029 0.0091 0.0044 0.0111 0.0087
Lipid Particles 0.0034 0.0001 0.0006 0.0004 0.002 0.0003 0.0102 0.0001 0.0066 0.0004 0.0001 0 0.018 0 0.0014 0.0009 0.0453 0.0006
Mitochondria 0.0158 0.0016 0.0054 0.0085 0.0149 0.0271 0.0165 0.0009 0.016 0.0021 0.006 0.0051 0.0059 0.0012 0.0092 0.0489 0.0086 0.0048
None 0.0019 0.0013 0.0011 0.001 0.0007 0.0014 0.008 0.0007 0.0009 0.0007 0.0007 0.0007 0.0043 0.0008 0.0013 0.0041 0.0009 0.0041
Nuclear Periphery 0.0055 0.0007 0.0021 0.0025 0.001 0.0012 0.0069 0.0013 0.0026 0.0075 0.0014 0.002 0.0075 0.0008 0.0025 0.0039 0.0032 0.0028
Nucleolus 0.0001 0 0.0002 0.0001 0 0.0001 0.0011 0 0.0001 0.0001 0 0 0.0002 0 0.0001 0.0004 0.0001 0.0002
Nucleus 0.0017 0.0008 0.001 0.0018 0.0009 0.0011 0.0025 0.0007 0.001 0.0019 0.0009 0.0009 0.0019 0.0007 0.0011 0.0026 0.0012 0.0011
Peroxisomes 0.0102 0.0001 0.0002 0.0005 0.0034 0.0003 0.0092 0.0001 0.0165 0.0005 0.0003 0.0001 0.0102 0 0.0017 0.0006 0.0183 0.0006
Punctate Nuclear 0.0017 0.0001 0.0004 0.0002 0.0003 0.0001 0.0038 0.0001 0.0003 0.0001 0.0001 0.0001 0.0138 0.0001 0.0008 0.0008 0.0009 0.002
Vacuole 0.0101 0.0046 0.009 0.0308 0.0063 0.0058 0.0113 0.0029 0.0084 0.0336 0.0058 0.0043 0.0049 0.0022 0.0056 0.0249 0.0189 0.0032
Vacuole Periphery 0.0037 0.0011 0.0034 0.0071 0.0033 0.0026 0.0033 0.0009 0.0028 0.0133 0.0049 0.0033 0.0018 0.0008 0.002 0.0102 0.0068 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.5843 16.7751 22.4061 30.2058 23.9246 22.506 27.7034 25.5666 24.7988 31.4596
Translational Efficiency 1.6645 2.3957 1.6175 1.1538 1.7269 2.3691 1.6518 1.589 1.7934 1.5924

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘