Standard name
Human Ortholog
Description Phosphotyrosine-specific protein phosphatase; major role in the pheromone adaptive response through dephosphorylation of the Fus3p MAPK with a minor role by Ptp2p and Msg5p; minor role in the inactivation of Hog1p MAPK during osmolarity sensing with major role by Ptp2p; dephosphorylates and regulates the localization of Hog1p; minor role in the cell wall integrity pathway through tyrosine dephosphorylation of the MAPK Slt2p while Ptp2p plays a major role; localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.92 0.94 0.98 0.98 0.97 0.95 0.91 0.86 – 0.8 0.74 0.99 0.99 0.99 0.97 0.97 0.98 0.96 0.8 0.85 0.92 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.29 0.13 0.09 0.05 0.18 0.19 0.21 0.39 – 0.44 0.5 0 0 0.05 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 1 1 0 0 0 0
Bud 1 0 1 0 1 4 4 7 10 – 5 2 1 5 6 0 0 0 0 1 0 0 1 5
Bud Neck 0 0 0 0 1 0 1 4 2 – 0 1 0 0 0 0 0 0 1 1 0 2 0 2
Bud Site 0 0 0 0 0 0 1 0 2 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 0 0 1 0 0 4 5 1 – 3 5 3 5 9 12 13 15 0 0 0 0 0 0
Cytoplasm 289 22 113 256 287 287 427 475 296 – 129 210 197 460 472 433 256 470 286 39 109 168 274 356
Endoplasmic Reticulum 2 0 3 0 1 0 0 0 0 – 0 2 1 0 0 21 13 26 1 1 2 0 2 7
Endosome 0 0 0 0 1 0 0 4 0 – 0 0 0 0 1 3 3 3 1 0 0 0 1 4
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Mitochondria 2 7 16 24 14 52 85 112 134 – 71 141 0 6 25 11 2 7 1 1 7 1 5 7
Nucleus 1 0 0 2 0 1 1 3 1 – 0 0 0 0 0 5 0 3 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 6 4 1 – 4 5 0 0 0 0 0 1 0 0 0 0 1 1
Vac/Vac Membrane 1 0 1 0 1 0 0 1 2 – 0 0 0 1 0 11 3 11 0 0 5 4 4 7
Unique Cell Count 295 24 120 261 293 297 451 522 344 162 283 198 467 478 446 263 481 299 50 130 183 298 401
Labelled Cell Count 304 29 134 283 306 346 529 616 449 212 367 202 478 514 496 292 538 299 50 130 183 298 401


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.6 3.7 3.9 4.0 3.3 3.0 3.3 2.7 – 2.7 2.4 5.8 5.7 4.7 6.2 6.6 6.8 5.6 5.3 5.2
Std Deviation (1e-4) 0.5 0.7 0.6 1.1 1.6 1.2 1.1 1.7 1.0 – 0.7 0.5 1.1 1.1 0.9 0.9 1.2 1.1 1.2 1.0 1.4
Intensity Change (Log2) – – – 0.08 0.11 -0.15 -0.28 -0.13 -0.46 – -0.44 -0.62 0.66 0.64 0.37 0.77 0.86 0.9 0.62 0.52 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2898 2.6092 2.4114 2.2783 2.0635 2.6636 2.1873 3.1997 2.9507 2.6001 2.9084 2.9776 0.9031 1.3563 1.0408 0.8077 0.9597 1.3177
Actin 0.0126 0.0003 0.0131 0.0008 0.0239 0.0193 0.0079 0.0028 0.0073 0.0003 0.0059 0.0023 0.04 0.0002 0.0083 0.0002 0.0005 0.0077
Bud 0.0017 0.0008 0.0045 0.0057 0.0022 0.0049 0.0007 0.0009 0.0031 0.0002 0.0006 0.0004 0.0005 0.0002 0.0031 0.0003 0.0002 0.0021
Bud Neck 0.0064 0.0003 0.0006 0.0008 0.0029 0.008 0.0005 0.0002 0.0003 0.0012 0.0004 0.0008 0.003 0.0002 0.0006 0.0003 0.0003 0.0022
Bud Periphery 0.0028 0.0007 0.007 0.0092 0.0082 0.0057 0.0009 0.0008 0.004 0.0001 0.0007 0.0005 0.0009 0.0002 0.0036 0.0002 0.0003 0.0037
Bud Site 0.0047 0.009 0.0027 0.0027 0.0059 0.004 0.0018 0.0074 0.0033 0.0012 0.0014 0.0002 0.0034 0.0012 0.0156 0.0004 0.0004 0.0021
Cell Periphery 0.0025 0.0015 0.0009 0.0013 0.0012 0.0009 0.0004 0.0003 0.0002 0 0.0001 0 0.0005 0.0003 0.0009 0.0001 0.0002 0.0003
Cytoplasm 0.6869 0.8097 0.7858 0.8174 0.7714 0.8346 0.7295 0.856 0.7966 0.925 0.7014 0.8743 0.5838 0.8246 0.7231 0.7862 0.6799 0.7199
Cytoplasmic Foci 0.0216 0.0065 0.0157 0.0163 0.0254 0.0159 0.0115 0.0089 0.0149 0.0228 0.0299 0.0135 0.0248 0.0067 0.0185 0.0107 0.0152 0.0164
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0 0 0 0 0 0.0005 0 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0082 0.0092 0.0052 0.0035 0.0022 0.0044 0.0147 0.0017 0.0018 0.0012 0.0025 0.0031 0.0076 0.0013 0.0043 0.0016 0.0029 0.0024
Endosome 0.0303 0.0042 0.0246 0.0135 0.0226 0.0086 0.0258 0.0058 0.0117 0.0086 0.0498 0.0112 0.0239 0.0035 0.013 0.0074 0.0202 0.0086
Golgi 0.0072 0.0004 0.0094 0.0006 0.0122 0.0037 0.0061 0.0016 0.0089 0.0008 0.0239 0.0061 0.0091 0.0003 0.0034 0.0003 0.0019 0.0014
Lipid Particles 0.0115 0.0003 0.0052 0.0009 0.011 0.0014 0.0014 0.0003 0.0023 0.0002 0.0428 0.0036 0.012 0.0001 0.0049 0.0001 0.0026 0.0013
Mitochondria 0.0236 0.0003 0.0041 0.0009 0.0225 0.0013 0.0031 0.0008 0.0232 0.0002 0.0359 0.0063 0.0107 0.0001 0.0027 0.0002 0.0052 0.0008
None 0.1546 0.1498 0.1067 0.1117 0.0662 0.0801 0.1635 0.1044 0.1071 0.0341 0.0844 0.06 0.2532 0.1584 0.1866 0.187 0.259 0.2264
Nuclear Periphery 0.0022 0.0007 0.0019 0.001 0.001 0.0009 0.0104 0.0011 0.0006 0.0003 0.0015 0.005 0.0028 0.0003 0.0011 0.0004 0.001 0.0004
Nucleolus 0.0005 0.0002 0.0002 0.0004 0.0011 0.0001 0.0003 0.0006 0.0001 0 0.0002 0.0001 0.0011 0 0.0002 0.0001 0.0008 0.0001
Nucleus 0.0027 0.0013 0.0015 0.0018 0.0027 0.0011 0.0034 0.002 0.0007 0.0007 0.0008 0.0032 0.0017 0.0005 0.0013 0.0008 0.0016 0.0005
Peroxisomes 0.0089 0 0.0021 0.0002 0.0096 0.0014 0.0004 0.0005 0.0081 0.0002 0.0108 0.0071 0.0082 0 0.0029 0.0001 0.0027 0.0017
Punctate Nuclear 0.0012 0.0004 0.0008 0.0009 0.003 0.0004 0.0101 0.0006 0.0002 0.0005 0.0028 0.0004 0.006 0.0001 0.0011 0.0002 0.0022 0.0007
Vacuole 0.0086 0.004 0.0067 0.0094 0.0036 0.0027 0.0063 0.0026 0.0041 0.002 0.0027 0.0013 0.0055 0.0016 0.0039 0.0032 0.0023 0.001
Vacuole Periphery 0.0013 0.0003 0.0011 0.0009 0.0009 0.0005 0.0014 0.0006 0.0014 0.0004 0.0015 0.0005 0.0008 0.0002 0.0006 0.0004 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8671 8.4941 5.5151 10.6707 8.2131 7.5181 11.0933 11.1101 9.7077 13.5764
Translational Efficiency 0.3642 0.6878 0.7886 0.4045 0.6247 0.7669 0.5503 0.4209 0.5465 0.5041

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1596 1423 2377 121 900 1738 22 149 2496 3161 2399 270

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.79 713.84 932.02 858.67 715.92 739.24 969.18 892.52 667.24 727.81 932.36 877.35
Standard Deviation 72.41 112.19 135.54 103.84 83.89 94.65 104.28 102.02 85.01 103.69 135.33 104.21
Intensity Change Log 2 — 0.158002 0.542762 0.424505 — 0.046244 0.436966 0.318086 — 0.100066 0.487862 0.369287

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000142 0.000344 0.000168 0.000189 0.000195 0.001041 0.000533 0.000331 0.000161 0.000727 0.000171 0.000267
Bud Neck 0.014898 0.020852 0.005193 0.001660 0.012025 0.023552 0.001044 0.006875 0.013862 0.022336 0.005155 0.004538
Bud Site 0.010334 0.024216 0.017375 0.006950 0.011656 0.023269 0.003031 0.026604 0.010811 0.023695 0.017244 0.017796
Cell Periphery 0.000173 0.000103 0.000081 0.000213 0.000258 0.000133 0.000400 0.000110 0.000204 0.000120 0.000084 0.000156
Cytoplasm 0.583474* 0.501060* 0.582723* 0.495309* 0.560645* 0.534560* 0.314545* 0.385062* 0.575242* 0.519479* 0.580263* 0.434469*
Cytoplasmic Foci 0.188999* 0.244712* 0.004934 0.105404 0.275909* 0.225999* 0.055628 0.069263 0.220337* 0.234423* 0.005399 0.085459
Eisosomes 0.000152 0.000117 0.000010 0.000107 0.000183 0.000169 0.000223 0.000073 0.000163 0.000146 0.000012 0.000088
Endoplasmic Reticulum 0.000594 0.000599 0.001216 0.001934 0.001450 0.000320 0.007230 0.001936 0.000903 0.000446 0.001271 0.001935
Endosome 0.021345 0.041350 0.000702 0.014512 0.024073 0.032663 0.019100 0.009387 0.022329 0.036574 0.000871 0.011684
Golgi 0.003035 0.007741 0.000066 0.000181 0.008003 0.009751 0.000177 0.000180 0.004827 0.008846 0.000067 0.000180
Lipid Particles 0.005089 0.004685 0.000320 0.004329 0.005738 0.002582 0.008863 0.001886 0.005323 0.003529 0.000398 0.002981
Mitochondria 0.002980 0.003780 0.000483 0.000121 0.004944 0.006709 0.000185 0.000518 0.003688 0.005391 0.000480 0.000340
Mitotic Spindle 0.001017 0.004400 0.003064 0.009035 0.001760 0.004749 0.000095 0.010831 0.001285 0.004592 0.003037 0.010026
None 0.005244 0.003489 0.005437 0.001055 0.001655 0.000657 0.000864 0.002350 0.003950 0.001932 0.005395 0.001770
Nuclear Periphery 0.000219 0.000388 0.000875 0.000896 0.000251 0.000084 0.001690 0.000711 0.000231 0.000221 0.000883 0.000794
Nuclear Periphery Foci 0.000523 0.000571 0.000791 0.004452 0.000383 0.000208 0.011164 0.001863 0.000473 0.000371 0.000886 0.003023
Nucleolus 0.001041 0.001974 0.000145 0.000399 0.000295 0.000440 0.000760 0.000690 0.000772 0.001130 0.000150 0.000560
Nucleus 0.051725 0.028020 0.345765* 0.106760 0.024135 0.023446 0.188403* 0.067344 0.041777 0.025505 0.344322* 0.085008
Peroxisomes 0.002279 0.007801 0.000078 0.000835 0.002776 0.006314 0.000867 0.000479 0.002458 0.006983 0.000085 0.000638
Vacuole 0.105146 0.100690 0.030334 0.245356* 0.061364 0.100448 0.384803* 0.406887* 0.089359 0.100557 0.033584 0.334497*
Vacuole Periphery 0.001592 0.003106 0.000240 0.000302 0.002304 0.002906 0.000397 0.006620 0.001848 0.002996 0.000242 0.003789

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.31 -1.85 -2.58 1.49 -0.14 -1.74 -2.89 -1.49 1.54 2.06 -2.11 -1.61 -2.91 1.67 -0.31
Bud Neck -3.67 8.67 12.93 14.46 4.78 -6.48 9.02 5.88 12.92 -2.32 -7.08 9.25 12.30 18.79 3.02
Bud Site -7.59 -2.17 1.35 6.45 2.70 -6.45 7.51 -0.56 2.12 -2.62 -10.01 -1.97 -0.07 4.73 1.12
Cell Periphery 2.47 3.14 -1.00 -5.28 -6.25 2.12 -1.65 2.99 3.69 5.01 2.98 4.16 2.23 -1.54 -4.47
Cytoplasm 8.44 7.34 6.03 2.65 3.68 2.47 6.24 10.07 9.15 -0.99 7.93 7.39 11.71 8.45 8.59
Cytoplasmic Foci -8.09 44.32 10.17 14.94 -9.88 6.27 22.96 27.11 24.64 0.68 -2.64 59.01 24.62 26.83 -11.37
Eisosomes 2.91 17.14 3.21 0.13 -11.74 0.81 -1.53 9.37 6.37 5.08 1.66 22.75 8.93 5.31 -14.74
Endoplasmic Reticulum -0.09 -11.04 -4.84 -4.81 -1.67 3.05 -3.41 -0.74 -6.41 3.29 3.14 -5.93 -4.72 -7.59 -0.88
Endosome -7.88 16.69 3.02 9.29 -7.75 -3.47 1.22 10.76 15.34 4.16 -8.30 21.33 9.69 16.50 -8.78
Golgi -6.60 11.95 11.37 11.36 -3.50 -1.73 13.86 13.98 12.08 1.92 -6.72 18.13 17.79 16.37 -4.19
Lipid Particles 0.47 10.10 0.93 0.55 -5.42 3.61 -2.15 4.85 4.16 5.41 3.58 11.22 4.69 1.87 -6.62
Mitochondria -0.93 3.29 3.84 7.63 7.34 -1.04 4.00 3.93 5.57 -0.50 -1.83 4.93 5.43 7.73 4.00
Mitotic Spindle -2.71 -3.06 -1.25 -0.63 -0.87 -2.12 2.08 -1.22 -0.42 -1.70 -3.60 -2.78 -1.80 -0.75 -1.21
None 1.43 0.22 4.71 2.29 10.96 3.49 2.20 -1.21 -2.19 -1.99 3.01 -1.98 2.20 -0.36 4.54
Nuclear Periphery -1.56 -21.22 -6.06 -4.07 1.08 1.55 -3.90 -5.77 -10.61 2.15 0.09 -18.48 -9.20 -9.02 2.37
Nuclear Periphery Foci -0.75 -5.06 -6.90 -6.66 -5.96 3.75 -5.01 -7.85 -9.01 4.40 1.05 -6.76 -8.52 -8.71 -6.01
Nucleolus -2.23 7.51 5.32 3.94 -4.31 -1.89 -2.81 0.04 1.18 2.37 -1.78 7.97 4.64 4.09 -2.57
Nucleus 9.70 -61.58 -4.14 -5.94 19.84 0.42 -4.53 -6.17 -6.26 2.91 10.60 -65.25 -6.39 -8.54 31.75
Peroxisomes -9.12 10.94 1.17 6.58 -1.52 -8.12 7.04 11.88 15.60 3.24 -12.42 15.85 4.38 12.68 -1.81
Vacuole 0.83 13.46 -8.76 -8.91 -10.93 -9.91 -6.90 -17.84 -16.23 -1.40 -3.87 9.83 -18.02 -17.38 -19.74
Vacuole Periphery -5.19 10.45 10.67 10.57 -1.26 -1.03 8.89 0.64 1.01 -0.81 -3.42 13.54 1.25 2.58 -1.01
Standard name
Human Ortholog
Description Phosphotyrosine-specific protein phosphatase; major role in the pheromone adaptive response through dephosphorylation of the Fus3p MAPK with a minor role by Ptp2p and Msg5p; minor role in the inactivation of Hog1p MAPK during osmolarity sensing with major role by Ptp2p; dephosphorylates and regulates the localization of Hog1p; minor role in the cell wall integrity pathway through tyrosine dephosphorylation of the MAPK Slt2p while Ptp2p plays a major role; localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ptp3

Ptp3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available