Standard name
Description Phosphotyrosine-specific protein phosphatase; major role in the pheromone adaptive response through dephosphorylation of the Fus3p MAPK with a minor role by Ptp2p and Msg5p; minor role in the inactivation of Hog1p MAPK during osmolarity sensing with major role by Ptp2p; dephosphorylates and regulates the localization of Hog1p; minor role in the cell wall integrity pathway through tyrosine dephosphorylation of the MAPK Slt2p while Ptp2p plays a major role; localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.92 0.94 0.98 0.98 0.97 0.95 0.91 0.86 0.8 0.74 0.99 0.99 0.99 0.97 0.97 0.98 0.96 0.8 0.85 0.92 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.29 0.13 0.09 0.05 0.18 0.19 0.21 0.39 0.44 0.5 0 0 0.05 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0
Bud 1 0 1 0 1 4 4 7 10 5 2 1 5 6 0 0 0 0 1 0 0 1 5
Bud Neck 0 0 0 0 1 0 1 4 2 0 1 0 0 0 0 0 0 1 1 0 2 0 2
Bud Site 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0
Cell Periphery 8 0 0 1 0 0 4 5 1 3 5 3 5 9 12 13 15 0 0 0 0 0 0
Cytoplasm 289 22 113 256 287 287 427 475 296 129 210 197 460 472 433 256 470 286 39 109 168 274 356
Endoplasmic Reticulum 2 0 3 0 1 0 0 0 0 0 2 1 0 0 21 13 26 1 1 2 0 2 7
Endosome 0 0 0 0 1 0 0 4 0 0 0 0 0 1 3 3 3 1 0 0 0 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Mitochondria 2 7 16 24 14 52 85 112 134 71 141 0 6 25 11 2 7 1 1 7 1 5 7
Nucleus 1 0 0 2 0 1 1 3 1 0 0 0 0 0 5 0 3 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 6 4 1 4 5 0 0 0 0 0 1 0 0 0 0 1 1
Vac/Vac Membrane 1 0 1 0 1 0 0 1 2 0 0 0 1 0 11 3 11 0 0 5 4 4 7
Unique Cell Count 295 24 120 261 293 297 451 522 344 162 283 198 467 478 446 263 481 299 50 130 183 298 401
Labelled Cell Count 304 29 134 283 306 346 529 616 449 212 367 202 478 514 496 292 538 299 50 130 183 298 401


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.6 3.7 3.9 4.0 3.3 3.0 3.3 2.7 2.7 2.4 5.8 5.7 4.7 6.2 6.6 6.8 5.6 5.3 5.2
Std Deviation (1e-4) 0.5 0.7 0.6 1.1 1.6 1.2 1.1 1.7 1.0 0.7 0.5 1.1 1.1 0.9 0.9 1.2 1.1 1.2 1.0 1.4
Intensity Change (Log2) 0.08 0.11 -0.15 -0.28 -0.13 -0.46 -0.44 -0.62 0.66 0.64 0.37 0.77 0.86 0.9 0.62 0.52 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available