Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 – 0 0 0 0 0.08 0.08 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0.05 0.06 0 0 0 0 – 0 0 0 0 0 0.06 0.05 – 0.1 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.06 0 0 – 0 0 0 0 0.13 0.23 0.22 – 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 – 0.06 0.05 0 0 0 0 0 0
Endosome 0 0.09 0.1 0 0.12 0.05 0.06 – 0 0 0 0 0.12 0.14 0.26 – 0.17 0.1 0.1 0.08 0.09 0.05 0.05 0.05
Golgi 0 0.08 0.2 0.05 0.05 0 0 – 0 0 0 0 0.13 0.07 0.05 – 0.14 0.26 0.05 0.08 0 0.06 0.05 0.05
Mitochondria 0.94 0.78 0.44 0.85 0.65 0.8 0.78 – 0.89 0.9 0.95 0.92 0.49 0.34 0.18 – 0.16 0.08 0.74 0.7 0.7 0.73 0.74 0.73
Nucleus 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0 0 0 0 0 – 0 0 0 0 0.07 0.11 0.28 – 0.09 0 0.06 0 0.09 0 0 0
SpindlePole 0 0 0.1 0.05 0.07 0.05 0.08 – 0.05 0.06 0 0.07 0 0 0.07 – 0.13 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0.19 0 0.08 0.06 0.08 – 0.07 0.07 0 0.05 0.1 0.1 0.11 – 0.25 0.31 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 8 17 4 8 2 3 – 0 3 0 3 15 18 1 – 2 0 7 14 4 6 7 9
Bud 5 15 2 7 6 9 6 – 14 9 9 12 4 6 3 – 3 0 0 2 1 0 2 3
Bud Neck 0 0 0 0 1 0 0 – 0 1 0 0 0 0 0 – 0 0 0 1 1 0 1 2
Bud Site 0 0 1 0 0 0 3 – 1 1 4 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 11 24 13 2 4 4 5 – 3 4 1 5 5 14 13 – 11 2 0 1 1 0 0 0
Cytoplasm 4 9 14 9 13 10 16 – 14 9 6 14 24 53 62 – 10 4 0 0 0 1 4 5
Endoplasmic Reticulum 6 12 9 5 8 10 9 – 1 1 8 7 7 11 10 – 7 2 0 2 1 0 0 5
Endosome 14 45 23 2 25 15 26 – 14 7 0 6 22 34 74 – 19 4 42 49 32 11 15 19
Golgi 2 43 45 7 11 2 3 – 4 0 0 2 23 17 13 – 16 10 21 50 12 12 16 20
Mitochondria 375 399 96 128 132 221 320 – 278 306 292 369 87 79 51 – 18 3 312 429 258 158 235 296
Nucleus 1 5 1 4 3 7 20 – 5 5 4 4 2 0 0 – 2 2 0 0 0 0 2 0
Nuclear Periphery 8 7 1 0 3 8 8 – 4 3 5 6 0 1 1 – 1 0 0 5 2 1 1 3
Nucleolus 7 8 0 0 0 4 3 – 1 2 5 5 2 0 1 – 1 1 0 3 1 0 0 0
Peroxisomes 10 27 8 4 8 6 7 – 5 3 0 3 13 26 79 – 10 1 23 24 33 9 13 18
SpindlePole 6 9 21 8 15 13 33 – 15 20 9 28 7 9 19 – 15 5 0 2 2 2 0 0
Vac/Vac Membrane 46 66 42 5 17 17 32 – 22 24 13 19 18 24 31 – 28 12 9 20 12 7 14 15
Unique Cell Count 401 513 220 151 204 275 411 313 341 306 402 178 235 283 112 39 422 614 369 217 320 405
Labelled Cell Count 495 677 293 185 254 328 494 381 398 356 486 229 292 358 143 46 422 614 369 217 320 405


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.2 6.5 5.5 6.1 5.2 5.4 – 4.9 4.9 4.2 4.7 6.6 6.5 6.4 – 7.6 7.1 6.1 6.3 6.4
Std Deviation (1e-4) 0.6 0.9 1.0 1.4 1.1 1.3 1.1 – 1.4 1.0 0.8 0.9 1.4 1.3 1.2 – 1.5 1.5 1.1 1.2 1.2
Intensity Change (Log2) – – – -0.24 -0.09 -0.31 -0.26 – -0.39 -0.41 -0.63 -0.45 0.02 0.0 -0.02 – 0.22 0.14 -0.08 -0.03 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.1 -1.7 -4.0 -4.8 – -5.0 0 0 0 0.3 0 -4.4 – -2.2 0
Bud 0 0 0 0 – 2.4 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery -2.2 -2.1 -2.7 -3.4 – -3.3 0 0 0 -1.5 0 -0.7 – 1.3 0
Cytoplasm -0.2 0 -1.4 -1.4 – -1.0 -2.2 -2.6 -1.7 2.4 4.9 4.8 – 0.9 0
Endoplasmic Reticulum -0.4 -0.1 -0.3 -1.4 – 0 0 0 0 -0.1 0.3 -0.3 – 0.9 0
Endosome -3.4 0.6 -2.1 -1.8 – -2.7 0 0 0 0.6 1.3 4.4 – 1.7 0
Golgi -4.3 -4.6 -7.4 -8.9 – -7.5 0 0 0 -2.0 -4.1 -5.5 – -1.4 0.7
Mitochondria 8.0 4.3 8.5 8.6 – 11.2 0 0 0 1.0 -2.2 -6.3 – -5.0 -4.3
Nucleus 0 0 0 2.9 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0.2 -1.0 -1.5 – -1.5 0 0 0 1.6 3.0 7.1 – 2.0 0
SpindlePole -1.5 -0.8 -2.1 -0.6 – -2.2 0 0 0 -2.2 -2.5 -1.2 – 1.1 0
Vacuole -4.5 -3.2 -4.4 -4.2 – -4.2 -4.3 -5.5 -5.8 -2.5 -2.7 -2.6 – 1.2 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9837 1.08 0.8654 0.4851 1.0411 0.9395 1.8003 1.7609 1.754 1.674 2.3714 1.7754 2.1877 2.0972 1.8857 2.3674 1.6606 2.0548
Actin 0.0414 0.0384 0.0192 0.0237 0.0028 0.0027 0.0144 0.0076 0.0068 0.0007 0.0108 0.0122 0.0029 0.0014 0.0021 0.002 0.0014 0.0048
Bud 0.0009 0.0026 0.0013 0.0019 0.0003 0.0004 0.0005 0.0019 0.001 0.0002 0.001 0.0004 0.0021 0.0011 0.0003 0.0001 0.0003 0.0017
Bud Neck 0.0035 0.0037 0.0034 0.0004 0.0003 0.0005 0.0008 0.001 0.0009 0.0001 0.001 0.0016 0.0004 0.0012 0.0007 0.0001 0.0002 0.0008
Bud Periphery 0.0015 0.0035 0.0034 0.0045 0.0013 0.0012 0.0012 0.0033 0.0016 0.0007 0.003 0.0009 0.0013 0.0018 0.001 0.0005 0.0008 0.0014
Bud Site 0.0125 0.0343 0.0103 0.0009 0.0004 0.0006 0.0027 0.0233 0.0073 0.0004 0.0035 0.0009 0.0019 0.0078 0.001 0.0002 0.0005 0.001
Cell Periphery 0.0011 0.0026 0.0019 0.0006 0.0004 0.0004 0.0005 0.0018 0.0004 0.0001 0.0007 0.0009 0.0003 0.0004 0.0002 0.0002 0.0001 0.0009
Cytoplasm 0.0088 0.0053 0.0123 0.0044 0.0004 0.0002 0.0048 0.008 0.0047 0.0008 0.0022 0.0031 0.0016 0.0027 0.0014 0.0003 0.0004 0.0022
Cytoplasmic Foci 0.0415 0.0352 0.0343 0.0097 0.0131 0.0046 0.0412 0.036 0.0266 0.0037 0.037 0.0192 0.0148 0.0194 0.0147 0.0055 0.012 0.0204
Eisosomes 0.0012 0.0008 0.001 0.0005 0.0002 0.0002 0.0005 0.0007 0.0003 0.0001 0.0006 0.0002 0.0002 0.0002 0.0001 0.0004 0.0001 0.0002
Endoplasmic Reticulum 0.0077 0.0042 0.0012 0.0027 0.0003 0.0004 0.0015 0.003 0.0005 0.0003 0.0041 0.0003 0.001 0.0005 0.0004 0.0001 0.0001 0.0002
Endosome 0.0888 0.0669 0.0447 0.0718 0.0844 0.0283 0.0807 0.0497 0.052 0.0352 0.1126 0.0237 0.0539 0.0521 0.0326 0.0055 0.0256 0.0311
Golgi 0.0549 0.0726 0.0556 0.0409 0.1027 0.0354 0.0714 0.0477 0.0419 0.0076 0.0698 0.0391 0.0493 0.0663 0.0349 0.0086 0.0797 0.0453
Lipid Particles 0.0745 0.0346 0.0178 0.0508 0.0235 0.0165 0.0584 0.0215 0.0152 0.003 0.0315 0.0075 0.0246 0.0123 0.0128 0.005 0.0055 0.0121
Mitochondria 0.5471 0.6259 0.7491 0.7693 0.7548 0.875 0.604 0.7023 0.7912 0.9284 0.6547 0.8476 0.7963 0.7804 0.8582 0.9183 0.8329 0.8254
None 0.0163 0.002 0.0065 0.0006 0.0003 0.0001 0.0241 0.0019 0.0051 0.0003 0.0047 0.0014 0.0008 0.0038 0.0004 0.0014 0.0001 0.0017
Nuclear Periphery 0.0074 0.0033 0.0005 0.0012 0.0001 0.0002 0.001 0.001 0.0003 0.0019 0.0064 0.0001 0.001 0.0002 0.0002 0.0001 0 0.0002
Nucleolus 0.0011 0.0005 0.0008 0.0001 0.0002 0.0002 0.0008 0.0006 0.0007 0.0004 0.004 0.0002 0.0015 0.0021 0.0004 0.0001 0.0001 0.0033
Nucleus 0.0014 0.0006 0.0009 0.0002 0.0002 0.0002 0.0007 0.0006 0.0007 0.001 0.0033 0.0002 0.0007 0.0008 0.0004 0.0001 0.0001 0.0005
Peroxisomes 0.0591 0.0462 0.0261 0.0082 0.0096 0.0255 0.0816 0.0793 0.0338 0.0087 0.0353 0.0374 0.0379 0.0387 0.03 0.0504 0.0383 0.0412
Punctate Nuclear 0.0034 0.0003 0.0008 0.0002 0.0001 0 0.0018 0.0005 0.0005 0.0007 0.0018 0.0003 0.0006 0.0006 0.0011 0 0 0.0009
Vacuole 0.018 0.0094 0.0054 0.0038 0.0021 0.003 0.0045 0.0054 0.0062 0.0036 0.007 0.0016 0.0035 0.0032 0.0036 0.0005 0.0006 0.0025
Vacuole Periphery 0.0079 0.0071 0.0036 0.0036 0.0026 0.0044 0.0028 0.0028 0.0023 0.0021 0.0051 0.0014 0.0035 0.0032 0.0036 0.0008 0.0011 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8044 7.158 6.0198 7.8413 6.5751 2.1341 9.2881 11.3979 8.0745 9.3656
Translational Efficiency 1.2627 1.1697 1.2312 0.8354 0.6401 1.9905 0.7625 0.7156 0.7517 0.8262

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrx1

Mrx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available