Standard name
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 – 0 0 0 0 0.08 0.08 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0.05 0.06 0 0 0 0 – 0 0 0 0 0 0.06 0.05 – 0.1 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.06 0 0 – 0 0 0 0 0.13 0.23 0.22 – 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 – 0.06 0.05 0 0 0 0 0 0
Endosome 0 0.09 0.1 0 0.12 0.05 0.06 – 0 0 0 0 0.12 0.14 0.26 – 0.17 0.1 0.1 0.08 0.09 0.05 0.05 0.05
Golgi 0 0.08 0.2 0.05 0.05 0 0 – 0 0 0 0 0.13 0.07 0.05 – 0.14 0.26 0.05 0.08 0 0.06 0.05 0.05
Mitochondria 0.94 0.78 0.44 0.85 0.65 0.8 0.78 – 0.89 0.9 0.95 0.92 0.49 0.34 0.18 – 0.16 0.08 0.74 0.7 0.7 0.73 0.74 0.73
Nucleus 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0 0 0 0 0 – 0 0 0 0 0.07 0.11 0.28 – 0.09 0 0.06 0 0.09 0 0 0
SpindlePole 0 0 0.1 0.05 0.07 0.05 0.08 – 0.05 0.06 0 0.07 0 0 0.07 – 0.13 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0.19 0 0.08 0.06 0.08 – 0.07 0.07 0 0.05 0.1 0.1 0.11 – 0.25 0.31 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 8 17 4 8 2 3 – 0 3 0 3 15 18 1 – 2 0 7 14 4 6 7 9
Bud 5 15 2 7 6 9 6 – 14 9 9 12 4 6 3 – 3 0 0 2 1 0 2 3
Bud Neck 0 0 0 0 1 0 0 – 0 1 0 0 0 0 0 – 0 0 0 1 1 0 1 2
Bud Site 0 0 1 0 0 0 3 – 1 1 4 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 11 24 13 2 4 4 5 – 3 4 1 5 5 14 13 – 11 2 0 1 1 0 0 0
Cytoplasm 4 9 14 9 13 10 16 – 14 9 6 14 24 53 62 – 10 4 0 0 0 1 4 5
Endoplasmic Reticulum 6 12 9 5 8 10 9 – 1 1 8 7 7 11 10 – 7 2 0 2 1 0 0 5
Endosome 14 45 23 2 25 15 26 – 14 7 0 6 22 34 74 – 19 4 42 49 32 11 15 19
Golgi 2 43 45 7 11 2 3 – 4 0 0 2 23 17 13 – 16 10 21 50 12 12 16 20
Mitochondria 375 399 96 128 132 221 320 – 278 306 292 369 87 79 51 – 18 3 312 429 258 158 235 296
Nucleus 1 5 1 4 3 7 20 – 5 5 4 4 2 0 0 – 2 2 0 0 0 0 2 0
Nuclear Periphery 8 7 1 0 3 8 8 – 4 3 5 6 0 1 1 – 1 0 0 5 2 1 1 3
Nucleolus 7 8 0 0 0 4 3 – 1 2 5 5 2 0 1 – 1 1 0 3 1 0 0 0
Peroxisomes 10 27 8 4 8 6 7 – 5 3 0 3 13 26 79 – 10 1 23 24 33 9 13 18
SpindlePole 6 9 21 8 15 13 33 – 15 20 9 28 7 9 19 – 15 5 0 2 2 2 0 0
Vac/Vac Membrane 46 66 42 5 17 17 32 – 22 24 13 19 18 24 31 – 28 12 9 20 12 7 14 15
Unique Cell Count 401 513 220 151 204 275 411 313 341 306 402 178 235 283 112 39 422 614 369 217 320 405
Labelled Cell Count 495 677 293 185 254 328 494 381 398 356 486 229 292 358 143 46 422 614 369 217 320 405


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.2 6.5 5.5 6.1 5.2 5.4 – 4.9 4.9 4.2 4.7 6.6 6.5 6.4 – 7.6 7.1 6.1 6.3 6.4
Std Deviation (1e-4) 0.6 0.9 1.0 1.4 1.1 1.3 1.1 – 1.4 1.0 0.8 0.9 1.4 1.3 1.2 – 1.5 1.5 1.1 1.2 1.2
Intensity Change (Log2) – – – -0.24 -0.09 -0.31 -0.26 – -0.39 -0.41 -0.63 -0.45 0.02 0.0 -0.02 – 0.22 0.14 -0.08 -0.03 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.1 -1.7 -4.0 -4.8 – -5.0 0 0 0 0.3 0 -4.4 – -2.2 0
Bud 0 0 0 0 – 2.4 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery -2.2 -2.1 -2.7 -3.4 – -3.3 0 0 0 -1.5 0 -0.7 – 1.3 0
Cytoplasm -0.2 0 -1.4 -1.4 – -1.0 -2.2 -2.6 -1.7 2.4 4.9 4.8 – 0.9 0
Endoplasmic Reticulum -0.4 -0.1 -0.3 -1.4 – 0 0 0 0 -0.1 0.3 -0.3 – 0.9 0
Endosome -3.4 0.6 -2.1 -1.8 – -2.7 0 0 0 0.6 1.3 4.4 – 1.7 0
Golgi -4.3 -4.6 -7.4 -8.9 – -7.5 0 0 0 -2.0 -4.1 -5.5 – -1.4 0.7
Mitochondria 8.0 4.3 8.5 8.6 – 11.2 0 0 0 1.0 -2.2 -6.3 – -5.0 -4.3
Nucleus 0 0 0 2.9 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0.2 -1.0 -1.5 – -1.5 0 0 0 1.6 3.0 7.1 – 2.0 0
SpindlePole -1.5 -0.8 -2.1 -0.6 – -2.2 0 0 0 -2.2 -2.5 -1.2 – 1.1 0
Vacuole -4.5 -3.2 -4.4 -4.2 – -4.2 -4.3 -5.5 -5.8 -2.5 -2.7 -2.6 – 1.2 1.7