Standard name
Description Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.09 0.05 0 0.05 0.06 0 0.06 0.06 0.05 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.11 0 0 0.08 0 0 0 0 0 0 0 0.11 0.12 0.18 0.26 0.18 0.18 0.08 0.11 0.08 0.08 0.09 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0.05 0 0.06 0.07 0.07
Mitochondria 0.9 0.75 0.9 0.88 0.88 0.92 0.96 0.96 0.95 0.93 0.97 0.96 0.78 0.73 0.67 0.05 0.07 0.06 0.71 0.57 0.67 0.59 0.55 0.51
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0.07 0.06 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.12 0.08 0.13 0.11 0 0 0 0 0 0 0 0.17 0.32 0.33 0.4 0.3 0.37 0.08 0.05 0.13 0.12 0.13 0.1
SpindlePole 0.07 0.06 0.08 0.07 0.07 0 0.05 0.05 0 0 0 0 0.07 0.1 0.07 0.31 0.27 0.26 0.05 0.05 0 0.05 0.07 0.07
Vac/Vac Membrane 0 0.16 0 0 0.1 0.1 0 0 0.06 0.06 0 0 0.08 0 0.11 0.15 0.15 0.11 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 2 1 5
Bud 7 4 6 10 7 4 11 13 7 10 12 9 5 14 5 24 0 6 0 6 0 1 3 3
Bud Neck 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 2 3 0 0 3 1 0 0 3
Bud Site 1 2 1 1 0 3 2 5 2 7 0 0 3 1 2 5 0 4 – – – – – –
Cell Periphery 0 8 3 5 1 2 0 0 1 1 2 4 5 6 6 11 11 9 0 0 0 0 0 0
Cytoplasm 1 8 4 4 3 4 5 3 5 5 7 3 10 3 10 24 10 6 0 1 1 0 0 1
Endoplasmic Reticulum 0 0 0 1 0 2 2 0 1 2 2 3 1 2 1 11 8 5 0 0 2 0 1 1
Endosome 4 34 3 1 11 1 1 0 3 0 0 0 37 43 53 78 32 21 13 33 11 15 19 27
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 4 5 5 7 22 4 5 16 4 10 15 20
Mitochondria 148 226 128 101 127 183 215 216 165 151 189 177 255 254 192 15 12 7 117 181 99 111 122 137
Nucleus 1 2 0 1 0 1 2 3 1 0 2 3 1 0 0 6 5 5 0 2 0 0 0 1
Nuclear Periphery 1 3 1 0 2 14 16 14 10 7 12 9 1 2 0 3 3 3 1 1 2 3 1 3
Nucleolus 10 10 2 2 5 7 7 11 4 3 1 3 9 5 1 8 2 3 0 1 1 2 2 1
Peroxisomes 11 37 12 15 16 3 2 1 1 0 1 0 54 111 94 118 54 44 13 14 19 22 29 28
SpindlePole 12 18 12 8 10 7 12 12 3 7 4 5 22 34 20 92 49 31 7 17 2 8 16 17
Vac/Vac Membrane 6 49 3 3 15 19 4 7 11 10 2 3 25 14 31 45 28 13 2 19 0 1 4 15
Unique Cell Count 165 300 142 115 144 200 223 224 174 162 195 184 327 350 288 297 182 119 166 316 148 188 224 273
Labelled Cell Count 202 404 175 152 197 250 279 285 214 203 235 219 432 495 420 449 239 161 166 316 148 188 224 273


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.1 4.9 5.2 4.9 4.3 3.4 3.9 4.1 4.1 3.5 3.4 6.8 6.4 6.7 6.4 8.6 8.5 5.9 6.4 6.8
Std Deviation (1e-4) 1.1 1.1 1.2 2.7 1.1 1.0 0.9 1.0 1.4 2.9 0.8 0.9 1.3 1.2 1.8 1.5 2.3 2.1 1.4 1.8 1.8
Intensity Change (Log2) – – – 0.1 0.03 -0.18 -0.51 -0.32 -0.25 -0.25 -0.47 -0.52 0.48 0.39 0.46 0.39 0.83 0.81 0.29 0.39 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 1.5 0.3 0 – – 0 0 – – 0 -0.1 0 1.5 0 0.3
Bud Neck 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Cell Periphery 0 0 0 – – 0 0 – – 0 0 0 0 1.7 2.1
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Endosome 0 2.2 0 – – 0 0 – – 3.3 3.5 4.7 6.1 4.5 4.3
Golgi 0 0 0 – – 0 0 – – 0 0 0 0 4.3 0
Mitochondria -0.6 -0.5 0 – – 0 0 – – -3.1 -4.2 -5.2 -17.8 -15.1 -13.6
Nuclear Periphery 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Peroxisomes 1.2 0.8 0 – – 0 0 – – 2.3 5.4 5.5 6.7 4.7 5.6
SpindlePole -0.4 -0.5 0 – – 0 0 – – -0.7 0.4 -0.6 5.2 4.2 3.8
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Cytoplasm 0 0 0 – – 0 0 – – 0 0 0 2.1 1.2 0
Nucleus 0 0 0 – – 0 0 – – 0 0 0 0 0 0
Vacuole 0 2.9 2.7 – – 1.8 1.7 – – 2.3 0 3.1 4.1 4.0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.8226 6.3062 5.9926 5.8457 5.3407 6.3036 5.4546 5.449 5.5101 5.348 5.5288 5.2562 5.5947 6.8795 6.6443 6.0269 6.4921 6.117
Actin 0.0042 0.0012 0.0022 0.0002 0.0027 0.0007 0.012 0.0065 0.01 0.0257 0.0017 0.006 0.003 0.0055 0.0176 0.014 0.0009 0.0136
Bud 0.0003 0.0004 0.0001 0.0001 0.0002 0 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0003
Bud Neck 0.0006 0.0001 0.0001 0 0.0007 0.0001 0.0003 0.0001 0.0002 0.0001 0 0.0003 0.0004 0.0003 0.0001 0.0003 0 0.0009
Bud Periphery 0.0007 0.0008 0.0003 0.0002 0.0008 0.0001 0.0008 0.0007 0.0004 0.0002 0.0002 0.0002 0.0009 0.0003 0.0004 0.0002 0.0003 0.0009
Bud Site 0.0027 0.0005 0.0002 0.0001 0.0002 0.0001 0.0012 0.0012 0.0003 0.0001 0.0001 0.0002 0.0015 0.0006 0.0006 0.0013 0.0001 0.0002
Cell Periphery 0.0005 0.0003 0.0003 0.0001 0.0003 0.0001 0.0003 0.0002 0.001 0 0 0.0002 0.0005 0.0001 0.0001 0.0001 0.0001 0.0002
Cytoplasm 0.0041 0.0003 0.0002 0 0.0005 0 0.0002 0 0.0001 0.0001 0 0.0002 0.0003 0.0001 0.0001 0.0001 0 0.0001
Cytoplasmic Foci 0.0105 0.0071 0.0077 0.0006 0.0115 0.001 0.007 0.0032 0.0044 0.0032 0.0011 0.0072 0.0129 0.0118 0.006 0.0062 0.0091 0.0108
Eisosomes 0.0004 0.0002 0.0001 0 0.0002 0.0001 0.0004 0.0003 0.0021 0.0001 0.0001 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0011 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003 0.0005 0.0005 0 0 0.0001 0.0004 0.0001 0.0001 0.0001 0 0.0003
Endosome 0.0239 0.0167 0.0051 0.0067 0.0147 0.0073 0.0241 0.0055 0.008 0.0051 0.0031 0.0148 0.026 0.0067 0.0073 0.0073 0.0036 0.0127
Golgi 0.0258 0.0139 0.0058 0.0051 0.0072 0.0068 0.0193 0.0136 0.0127 0.0382 0.0084 0.0315 0.0116 0.0169 0.0441 0.0333 0.0052 0.033
Lipid Particles 0.0336 0.0376 0.006 0.0015 0.0101 0.008 0.0267 0.0061 0.0117 0.0015 0.0012 0.0158 0.0323 0.0264 0.007 0.0073 0.033 0.021
Mitochondria 0.8345 0.8856 0.9528 0.9823 0.9036 0.9628 0.8569 0.907 0.9291 0.9196 0.9772 0.8926 0.8819 0.8109 0.8652 0.8906 0.9196 0.8808
None 0.0067 0.0001 0.0001 0 0.0188 0 0.0006 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0005 0.0001 0 0.0002
Nuclear Periphery 0.0076 0.0004 0.0002 0 0.0002 0.0001 0.0004 0.0011 0.0004 0 0 0 0.0002 0.0002 0.0004 0 0 0.0002
Nucleolus 0.0035 0.0002 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0001 0 0 0 0.0008 0.0003 0.0001 0.0001 0.0005 0.0002
Nucleus 0.0029 0.0002 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0 0.0005 0.0001 0.0001 0 0.0001 0.0001
Peroxisomes 0.027 0.024 0.0116 0.0009 0.0121 0.0096 0.0395 0.0471 0.016 0.005 0.0058 0.0287 0.0163 0.1166 0.0474 0.0376 0.023 0.0181
Punctate Nuclear 0.0018 0 0.0026 0 0.0013 0 0.0002 0 0 0 0 0 0.0002 0.0001 0.0004 0.0001 0 0.0002
Vacuole 0.0038 0.003 0.0018 0.0008 0.0063 0.0008 0.0059 0.0014 0.001 0.0002 0.0002 0.0005 0.0063 0.0007 0.0004 0.0003 0.001 0.0025
Vacuole Periphery 0.0037 0.0071 0.0025 0.0013 0.0083 0.0022 0.0031 0.0048 0.0017 0.0005 0.0006 0.0011 0.0029 0.002 0.0018 0.0006 0.0033 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0856 10.2748 14.7721 16.2637 12.108 7.1796 17.3688 17.5293 15.3265 15.0093
Translational Efficiency 0.9247 1.0688 0.6754 0.5899 0.6985 1.9137 0.7124 0.5387 0.6311 0.5752

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘