ORF
Human Ortholog
Description Putative protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 – 0.96 0.73 0.7 0.71 – 0.62 0.55 0.49 0.58 0.99 1.0 0.98 0.87 0.93 0.91 0.92 0.88 0.84 0.94 0.91 0.92
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.18 0 – 0.23 0.21 0.28 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0.06 0.1 – 0.06 0.12 0 0.11 0 0 0 0 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0.29 0.13 0.27 – 0.2 0.22 0.3 0.16 0 0 0 0.13 0.08 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 3
Bud 1 0 – 1 0 1 0 – 1 1 5 2 1 0 0 0 0 1 1 1 0 3 6 5
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 1 1 – 3 2 3 3 0 0 3 3 8 4 0 0 0 0 0 0
Cytoplasm 264 208 – 53 119 88 180 – 118 118 75 83 266 418 431 104 108 136 258 196 42 277 328 311
Endoplasmic Reticulum 2 2 – 0 0 1 2 – 1 2 0 2 0 1 2 4 2 5 0 0 1 0 2 0
Endosome 0 0 – 0 0 0 2 – 0 0 2 0 1 0 0 1 0 2 2 1 0 0 1 1
Golgi 3 0 – 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 2 0 0 0 2 2
Mitochondria 1 4 – 1 1 23 6 – 43 46 42 33 1 1 1 2 1 2 1 4 0 4 1 3
Nucleus 3 1 – 2 5 7 26 – 11 27 4 16 0 2 1 3 1 1 7 10 1 1 1 0
Nuclear Periphery 1 0 – 0 0 0 0 – 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 1 0 – 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Vac/Vac Membrane 5 3 – 0 47 16 69 – 37 48 45 23 3 1 16 16 9 13 4 4 1 1 5 1
Unique Cell Count 272 216 55 163 125 252 189 216 152 142 269 420 438 120 116 150 281 223 51 294 362 339
Labelled Cell Count 280 218 57 172 138 286 215 244 176 164 272 423 455 134 130 166 281 223 51 294 362 339


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.1 – 5.3 8.2 5.7 8.3 – 7.1 7.3 7.2 6.6 6.4 7.4 8.2 10.6 11.6 11.3 5.5 5.8 5.8
Std Deviation (1e-4) 1.0 1.0 – 0.9 1.5 1.4 1.7 – 2.1 1.7 3.1 1.7 1.2 1.6 1.8 2.6 2.3 2.1 1.7 2.2 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0109 1.7265 1.084 1.1338 0.5482 1.2158 2.817 3.8918 3.1347 2.5887 2.9212 3.72 4.5155 5.7614 4.7665 4.6772 4.6084 4.7496
Actin 0.0039 0.0001 0.0025 0.0067 0.0203 0.0017 0.0173 0.0003 0.0239 0.0342 0.0212 0.0056 0.0076 0.0005 0.0014 0.0016 0.0005 0.0004
Bud 0.0014 0.0001 0.007 0.0233 0.0035 0.0007 0.0004 0.0001 0.0013 0.0002 0.0003 0.0005 0.0001 0.0001 0.0001 0.0007 0.0001 0.0001
Bud Neck 0.002 0.0002 0.0004 0.0072 0.0013 0.0015 0.0029 0.0002 0.0012 0.0004 0.0009 0.0038 0.0012 0.0003 0.0004 0.0006 0.0003 0.0011
Bud Periphery 0.0011 0 0.0015 0.0038 0.0056 0.0003 0.0002 0 0.0029 0.0002 0.0003 0.0003 0.0001 0 0 0.0004 0 0
Bud Site 0.0016 0.0004 0.0019 0.0034 0.0015 0.0002 0.0024 0.0003 0.0025 0.0002 0.0006 0.0005 0.0004 0.0004 0.0004 0.0006 0.0002 0.0001
Cell Periphery 0.0001 0 0.0003 0.0011 0.0002 0 0.0001 0 0.0002 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0
Cytoplasm 0.8001 0.852 0.7809 0.659 0.7059 0.8248 0.7894 0.9448 0.8506 0.8589 0.8599 0.8188 0.8118 0.8277 0.8737 0.7665 0.743 0.8529
Cytoplasmic Foci 0.0251 0.0036 0.0092 0.0142 0.0173 0.0108 0.041 0.0021 0.0076 0.0034 0.011 0.0189 0.0051 0.0015 0.0054 0.0087 0.0067 0.0051
Eisosomes 0 0 0.0002 0.0003 0.0001 0 0.0001 0 0.0001 0.0003 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.006 0.0026 0.0063 0.0056 0.0047 0.0026 0.0081 0.0033 0.0036 0.004 0.0016 0.0028 0.006 0.0012 0.0036 0.0032 0.0032 0.0026
Endosome 0.0181 0.0021 0.007 0.0363 0.0309 0.0119 0.0129 0.0011 0.0108 0.0039 0.0077 0.0083 0.0039 0.0003 0.0029 0.0034 0.0023 0.0034
Golgi 0.0027 0.0001 0.0007 0.0055 0.0112 0.0018 0.0039 0.0001 0.0045 0.0052 0.0118 0.0023 0.0007 0 0.0002 0.0003 0.0001 0.0002
Lipid Particles 0.0024 0.0001 0.0007 0.0035 0.0179 0.004 0.0072 0.0001 0.007 0.0041 0.0056 0.0122 0.0004 0 0.0001 0.0002 0.0001 0.0001
Mitochondria 0.0028 0.0001 0.0059 0.023 0.026 0.0022 0.0006 0.0001 0.0134 0.002 0.0151 0.0006 0.0003 0.0001 0.0002 0.0005 0.0002 0.0002
None 0.0861 0.0968 0.1274 0.1222 0.0969 0.0803 0.0754 0.0255 0.0365 0.0523 0.0288 0.0552 0.1341 0.1223 0.0863 0.188 0.2295 0.0897
Nuclear Periphery 0.0064 0.0026 0.0099 0.0112 0.0058 0.0039 0.0067 0.0024 0.0046 0.0022 0.0013 0.0026 0.0034 0.0017 0.0019 0.0034 0.0017 0.002
Nucleolus 0.0007 0.0001 0.0002 0.004 0.0004 0.0003 0.0002 0 0.0006 0.0001 0.0001 0.0005 0.0001 0 0.0001 0.0002 0.0001 0.0001
Nucleus 0.0237 0.036 0.0309 0.0497 0.0272 0.0413 0.0099 0.0178 0.0172 0.0251 0.0133 0.0525 0.0215 0.0428 0.0197 0.0135 0.0096 0.0383
Peroxisomes 0.0041 0 0.0011 0.0015 0.0156 0.0013 0.0112 0 0.0024 0.0016 0.0161 0.0043 0.0003 0.0001 0.0001 0.0005 0.0002 0.0001
Punctate Nuclear 0.008 0.0016 0.0034 0.0112 0.0039 0.0086 0.0087 0.0011 0.006 0.001 0.0034 0.0096 0.0019 0.0006 0.0025 0.0065 0.0015 0.0029
Vacuole 0.0029 0.0013 0.0019 0.005 0.0029 0.0014 0.0011 0.0006 0.0022 0.0005 0.0006 0.0007 0.0008 0.0003 0.0008 0.0008 0.0005 0.0005
Vacuole Periphery 0.0007 0.0001 0.0006 0.0023 0.001 0.0004 0.0003 0.0001 0.0007 0.0003 0.0002 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
223 855 214 1815 1599 2494 309 1931 1822 3349 523 3746

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 790.39 838.21 1315.64 912.99 714.33 818.61 1024.65 1007.96 723.64 823.61 1143.72 961.95
Standard Deviation 113.38 155.74 164.10 177.41 89.32 102.65 141.78 163.74 95.90 118.80 208.24 176.98
Intensity Change Log 2 — 0.084747 0.735128 0.208034 — 0.196586 0.520469 0.496776 — 0.138922 0.637192 0.352325

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000582 0.002137 0.001252 0.001946 0.000081 0.000750 0.000578 0.000323 0.000142 0.001104 0.000854 0.001109
Bud Neck 0.006618 0.013413 0.005654 0.005144 0.007044 0.017783 0.001784 0.004196 0.006992 0.016668 0.003368 0.004655
Bud Site 0.017701 0.019951 0.006619 0.032281 0.003886 0.018540 0.004074 0.014650 0.005577 0.018900 0.005115 0.023193
Cell Periphery 0.000711 0.000154 0.000359 0.000213 0.000147 0.000135 0.000178 0.000126 0.000216 0.000140 0.000252 0.000168
Cytoplasm 0.540789* 0.551371* 0.254363* 0.567268* 0.550128* 0.571382* 0.446201* 0.632580* 0.548985* 0.566273* 0.367705* 0.600935*
Cytoplasmic Foci 0.108894 0.157699 0.006641 0.012780 0.139852 0.090631 0.009206 0.011004 0.136063 0.107753 0.008157 0.011865
Eisosomes 0.000098 0.000069 0.000039 0.000039 0.000081 0.000059 0.000043 0.000022 0.000083 0.000062 0.000041 0.000030
Endoplasmic Reticulum 0.006467 0.001234 0.006486 0.003115 0.001135 0.000489 0.004831 0.001043 0.001788 0.000679 0.005508 0.002047
Endosome 0.004073 0.009873 0.002781 0.002080 0.005953 0.005197 0.002331 0.001858 0.005723 0.006391 0.002515 0.001966
Golgi 0.000516 0.003091 0.000275 0.000487 0.001089 0.002127 0.000108 0.000548 0.001019 0.002373 0.000177 0.000519
Lipid Particles 0.003011 0.003320 0.001826 0.000942 0.004738 0.002164 0.001375 0.000597 0.004527 0.002459 0.001559 0.000764
Mitochondria 0.001081 0.003260 0.000724 0.001200 0.002528 0.004473 0.000280 0.002755 0.002350 0.004164 0.000462 0.002001
Mitotic Spindle 0.002840 0.004688 0.009846 0.013883 0.001761 0.008699 0.000516 0.014185 0.001893 0.007675 0.004334 0.014039
None 0.004335 0.004851 0.003433 0.005868 0.004845 0.004326 0.006025 0.007919 0.004782 0.004460 0.004964 0.006925
Nuclear Periphery 0.000473 0.000346 0.002019 0.001155 0.000427 0.000361 0.000988 0.000655 0.000433 0.000357 0.001410 0.000897
Nuclear Periphery Foci 0.003891 0.001260 0.004477 0.001661 0.000581 0.000319 0.004338 0.001245 0.000986 0.000559 0.004395 0.001447
Nucleolus 0.000682 0.000913 0.000249 0.000334 0.000837 0.001061 0.000207 0.000213 0.000818 0.001024 0.000224 0.000272
Nucleus 0.256809* 0.159319* 0.602494* 0.237326* 0.242380* 0.231661* 0.417028* 0.235563* 0.244146* 0.213192* 0.492916* 0.236417*
Peroxisomes 0.001536 0.008339 0.000219 0.001007 0.002517 0.005913 0.000155 0.000281 0.002396 0.006533 0.000181 0.000632
Vacuole 0.038404 0.053093 0.090035 0.109245 0.029210 0.033184 0.099625 0.069342 0.030335 0.038267 0.095701 0.088676
Vacuole Periphery 0.000492 0.001617 0.000206 0.002025 0.000780 0.000746 0.000129 0.000893 0.000745 0.000969 0.000161 0.001442

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.44 -6.21 -0.94 1.08 2.24 -2.48 -9.67 -5.17 1.44 4.07 -2.87 -12.90 -3.08 1.25 2.72
Bud Neck -3.73 -0.76 1.40 5.77 3.60 -7.32 5.85 3.41 10.50 -5.20 -7.97 3.29 3.32 11.71 -0.12
Bud Site -0.47 2.50 0.22 1.00 -4.29 -8.22 -0.90 -3.80 5.00 -3.08 -8.41 -0.56 -5.35 3.63 -5.48
Cell Periphery 6.55 2.85 6.54 -0.10 8.27 1.45 -2.31 3.15 1.75 4.15 5.55 -3.64 5.70 0.38 8.52
Cytoplasm -0.45 14.84 4.99 8.87 -16.13 -2.43 12.59 5.50 8.42 -9.58 -2.05 21.11 10.12 14.02 -15.73
Cytoplasmic Foci -4.40 10.96 10.64 22.94 -3.37 9.65 30.83 31.04 28.02 -0.48 5.97 32.97 32.75 35.62 -2.31
Eisosomes 2.95 4.29 5.39 5.83 1.88 5.30 4.53 13.53 11.60 6.15 5.65 6.16 12.94 11.25 5.72
Endoplasmic Reticulum 5.88 -1.05 3.77 -9.93 6.37 4.06 -9.31 -1.83 -11.13 9.03 6.09 -10.86 -2.44 -16.35 10.43
Endosome -4.32 0.74 3.47 8.23 6.38 1.19 4.93 7.31 8.60 4.34 -0.64 4.62 7.72 11.83 7.18
Golgi -3.27 1.40 2.37 3.70 2.05 -2.30 5.26 4.03 4.60 -1.02 -3.36 4.96 4.60 5.94 -0.13
Lipid Particles -0.19 0.87 3.46 2.88 6.95 5.01 7.73 10.66 3.77 5.20 4.51 7.09 10.74 4.46 8.15
Mitochondria -1.66 0.58 0.71 1.94 0.47 -2.17 3.95 1.61 3.64 -1.77 -2.28 3.69 2.31 4.66 -1.79
Mitotic Spindle -0.85 -1.58 -4.22 -3.36 -0.47 -4.44 -0.17 -5.12 -0.84 -4.74 -4.48 -1.69 -7.17 -2.11 -2.98
None -0.34 1.43 -1.52 -0.88 -4.61 0.86 -0.11 -5.03 -6.30 -4.69 0.64 1.23 -3.85 -4.73 -6.05
Nuclear Periphery 1.17 -12.83 -12.49 -25.06 5.37 1.11 -12.33 -18.94 -15.15 2.32 1.57 -18.16 -27.04 -25.01 4.71
Nuclear Periphery Foci 2.05 -0.63 1.88 -1.38 5.81 2.49 -7.17 -4.25 -5.19 6.01 2.27 -8.54 -1.86 -6.76 8.19
Nucleolus -1.78 3.22 3.26 5.98 0.41 -1.18 4.75 4.66 4.87 -0.60 -1.32 4.86 4.78 5.81 -0.02
Nucleus 5.88 -13.28 -2.44 -16.71 14.86 1.49 -11.68 -9.44 -11.67 7.24 4.85 -18.87 -9.85 -17.38 14.52
Peroxisomes -5.40 4.32 3.23 6.43 -0.98 -3.93 4.98 4.97 8.10 -0.05 -5.46 5.27 4.96 10.06 -1.05
Vacuole -2.76 -9.32 -21.18 -22.38 -2.64 -2.59 -11.26 -23.00 -21.79 2.97 -5.08 -15.19 -36.19 -33.52 0.58
Vacuole Periphery -1.58 3.04 -0.62 1.13 -1.32 0.14 3.35 1.39 1.24 -0.88 -0.73 3.32 0.82 1.38 -1.63
ORF
Human Ortholog
Description Putative protein of unknown function
Localization
Cell Percentages cytoplasm (53%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YER079W

YER079W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yer079w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available