Standard name | |
---|---|
Description | Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 7 | 1 | 1 | 1 | 1 | – | 2 | 2 | – | – | – | 0 | 5 | 1 | 0 | 6 | 2 |
Bud | 1 | 1 | 2 | 1 | 1 | 3 | 5 | 6 | 12 | 7 | 11 | 5 | – | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 12 | 4 | 8 | 5 | 4 | 4 | 6 | 2 | 8 | 3 | 8 | – | 1 | 7 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 1 | 0 | 5 | 4 | 7 | 3 | 2 | 4 | 4 | 6 | – | 8 | 6 | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 2 | 1 | 2 | 0 | 5 | – | 1 | 2 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 8 | 2 | 8 | 13 | 17 | 23 | 16 | 7 | 11 | 0 | 7 | – | 5 | 3 | – | – | – | 2 | 15 | 6 | 1 | 1 | 2 |
Golgi | 0 | 34 | 22 | 43 | 65 | 18 | 15 | 25 | 9 | 19 | 2 | 13 | – | 54 | 65 | – | – | – | 3 | 21 | 3 | 0 | 13 | 16 |
Mitochondria | 105 | 234 | 60 | 144 | 167 | 230 | 324 | 348 | 252 | 345 | 338 | 404 | – | 95 | 100 | – | – | – | 96 | 235 | 91 | 27 | 76 | 99 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | – | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 8 | 6 | 0 | 3 | 10 | 29 | 53 | 51 | 32 | 38 | 27 | 41 | – | 2 | 4 | – | – | – | 0 | 2 | 0 | 0 | 1 | 0 |
Nucleolus | 11 | 7 | 1 | 5 | 11 | 21 | 53 | 42 | 37 | 29 | 23 | 25 | – | 2 | 1 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 7 | 4 | 8 | 7 | 8 | 8 | 8 | 12 | 3 | 0 | 2 | – | 2 | 1 | – | – | – | 3 | 6 | 6 | 1 | 4 | 4 |
SpindlePole | 0 | 0 | 2 | 5 | 2 | 5 | 4 | 7 | 8 | 7 | 1 | 8 | – | 1 | 1 | – | – | – | 0 | 2 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 11 | 58 | 23 | 14 | 40 | 25 | 22 | 18 | 20 | 32 | 14 | 13 | – | 18 | 14 | – | – | – | 1 | 17 | 4 | 0 | 1 | 8 |
Unique Cell Count | 113 | 279 | 81 | 167 | 240 | 297 | 425 | 436 | 308 | 406 | 352 | 444 | 130 | 140 | 110 | 314 | 116 | 34 | 109 | 142 | ||||
Labelled Cell Count | 139 | 372 | 122 | 242 | 328 | 367 | 525 | 542 | 399 | 509 | 424 | 538 | 191 | 206 | 110 | 314 | 116 | 34 | 109 | 142 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 9.0 | 8.8 | 9.2 | 11.2 | 10.5 | 10.3 | 10.1 | 8.9 | 9.3 | 8.2 | 8.9 | – | 10.3 | 10.3 | – | – | – | – | 10.2 | 11.2 |
Std Deviation (1e-4) | 1.4 | 1.8 | 2.1 | 1.9 | 2.1 | 2.8 | 2.7 | 2.3 | 2.5 | 2.2 | 2.3 | 2.5 | – | 1.9 | 2.0 | – | – | – | – | 1.8 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.35 | 0.25 | 0.22 | 0.19 | 0.02 | 0.08 | -0.11 | 0.01 | – | 0.23 | 0.22 | – | – | – | – | 0.21 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Golgi | -0.2 | 0 | -5.5 | -7.5 | -6.2 | -7.2 | -6.7 | 0 | -8.0 | – | 2.1 | 2.8 | – | – | – |
Mitochondria | 2.3 | -0.8 | 0.6 | 0.4 | 1.2 | 1.6 | 2.4 | 6.5 | 4.4 | – | -0.2 | -0.4 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 2.9 | 3.4 | 3.2 | 3.0 | 2.9 | 2.6 | 2.8 | – | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 3.0 | 2.5 | 2.9 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Vacuole | -4.1 | -2.3 | -4.8 | -6.7 | -7.4 | -5.6 | -5.3 | -7.1 | -8.3 | – | -2.6 | -3.5 | – | – | – |