Standard name
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – – – – –
Cell Periphery 0 0 0.05 0.05 0 0 0 0 0 0 0 0 – 0 0.05 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 – – – 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0 – 0 0 – – – 0 0.05 0.06 0 0 0
Golgi 0 0.12 0.27 0.26 0.27 0.06 0 0.06 0 0.05 0 0 – 0.42 0.46 – – – 0 0.07 0 0 0.13 0.11
Mitochondria 0.93 0.84 0.74 0.86 0.7 0.77 0.76 0.8 0.82 0.85 0.96 0.91 – 0.73 0.71 – – – 0.88 0.75 0.79 0.81 0.7 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0.1 0.12 0.12 0.1 0.09 0.08 0.09 – 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0.05 0.07 0.12 0.1 0.12 0.07 0.07 0.06 – 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.05 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.21 0.28 0.08 0.17 0.08 0.05 0 0.06 0.08 0 0 – 0.14 0.1 – – – 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 3 1 1 1 7 1 1 1 1 – 2 2 – – – 0 5 1 0 6 2
Bud 1 1 2 1 1 3 5 6 12 7 11 5 – 0 0 – – – 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 3 1 0 0 – 0 0 – – – – – – – – –
Cell Periphery 1 12 4 8 5 4 4 6 2 8 3 8 – 1 7 – – – 0 0 0 0 0 0
Cytoplasm 1 3 1 0 5 4 7 3 2 4 4 6 – 8 6 – – – 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 1 0 1 1 4 2 1 2 0 5 – 1 2 – – – 0 1 0 0 0 0
Endosome 0 8 2 8 13 17 23 16 7 11 0 7 – 5 3 – – – 2 15 6 1 1 2
Golgi 0 34 22 43 65 18 15 25 9 19 2 13 – 54 65 – – – 3 21 3 0 13 16
Mitochondria 105 234 60 144 167 230 324 348 252 345 338 404 – 95 100 – – – 96 235 91 27 76 99
Nucleus 1 1 0 0 0 1 2 2 1 2 0 0 – 0 0 – – – 0 0 0 1 0 0
Nuclear Periphery 8 6 0 3 10 29 53 51 32 38 27 41 – 2 4 – – – 0 2 0 0 1 0
Nucleolus 11 7 1 5 11 21 53 42 37 29 23 25 – 2 1 – – – 0 1 0 0 0 0
Peroxisomes 0 7 4 8 7 8 8 8 12 3 0 2 – 2 1 – – – 3 6 6 1 4 4
SpindlePole 0 0 2 5 2 5 4 7 8 7 1 8 – 1 1 – – – 0 2 0 0 0 0
Vac/Vac Membrane 11 58 23 14 40 25 22 18 20 32 14 13 – 18 14 – – – 1 17 4 0 1 8
Unique Cell Count 113 279 81 167 240 297 425 436 308 406 352 444 130 140 110 314 116 34 109 142
Labelled Cell Count 139 372 122 242 328 367 525 542 399 509 424 538 191 206 110 314 116 34 109 142


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 9.0 8.8 9.2 11.2 10.5 10.3 10.1 8.9 9.3 8.2 8.9 – 10.3 10.3 – – – – 10.2 11.2
Std Deviation (1e-4) 1.4 1.8 2.1 1.9 2.1 2.8 2.7 2.3 2.5 2.2 2.3 2.5 – 1.9 2.0 – – – – 1.8 2.3
Intensity Change (Log2) – – – 0.06 0.35 0.25 0.22 0.19 0.02 0.08 -0.11 0.01 – 0.23 0.22 – – – – 0.21 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Golgi -0.2 0 -5.5 -7.5 -6.2 -7.2 -6.7 0 -8.0 – 2.1 2.8 – – –
Mitochondria 2.3 -0.8 0.6 0.4 1.2 1.6 2.4 6.5 4.4 – -0.2 -0.4 – – –
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Nuclear Periphery 0 0 2.9 3.4 3.2 3.0 2.9 2.6 2.8 – 0 0 – – –
Nucleolus 0 0 0 3.0 2.5 2.9 0 0 0 – 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Vacuole -4.1 -2.3 -4.8 -6.7 -7.4 -5.6 -5.3 -7.1 -8.3 – -2.6 -3.5 – – –