Standard name | |
---|---|
Human Ortholog | |
Description | Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 5 | 2 | 3 | 1 | 2 | 21 | 15 | 11 | 4 | 11 | 10 |
Bud | 2 | 0 | 0 | 2 | 2 | 3 | 1 | 1 | 3 | 1 | 5 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 4 | 4 | 0 | 2 | 2 |
Bud Neck | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 5 | 0 | 0 | 0 | 2 | 3 | 0 | 9 | 13 | 9 | 6 | 7 | 12 |
Bud Site | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 2 | 8 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 3 | 2 | 8 | 2 | 10 | 8 | 5 | 4 | 5 | 2 | 5 | 4 | 3 | 9 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 3 | 1 | 4 | 1 | 5 | 3 | 5 | 2 | 2 | 1 | 2 | 3 | 1 | 0 | 1 | 6 | 6 | 3 | 14 | 10 | 7 | 2 | 7 | 6 |
Golgi | 0 | 8 | 10 | 1 | 6 | 2 | 2 | 2 | 0 | 1 | 0 | 1 | 4 | 10 | 14 | 35 | 18 | 20 | 13 | 19 | 15 | 6 | 16 | 25 |
Mitochondria | 83 | 72 | 27 | 35 | 50 | 83 | 96 | 93 | 81 | 69 | 101 | 95 | 54 | 41 | 44 | 43 | 39 | 24 | 54 | 74 | 31 | 8 | 19 | 31 |
Nucleus | 1 | 1 | 0 | 1 | 1 | 0 | 4 | 5 | 1 | 3 | 1 | 4 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 2 | 0 | 7 | 6 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 49 | 40 | 20 | 39 | 78 | 72 | 105 | 111 | 84 | 67 | 83 | 101 | 14 | 23 | 9 | 50 | 46 | 47 | 12 | 14 | 8 | 5 | 23 | 21 |
Peroxisomes | 28 | 19 | 30 | 23 | 25 | 11 | 24 | 18 | 17 | 10 | 5 | 15 | 8 | 4 | 19 | 102 | 75 | 47 | 36 | 22 | 29 | 9 | 20 | 37 |
SpindlePole | 12 | 11 | 22 | 26 | 10 | 8 | 10 | 11 | 9 | 8 | 9 | 16 | 2 | 9 | 3 | 76 | 59 | 61 | 27 | 14 | 18 | 7 | 25 | 23 |
Vac/Vac Membrane | 2 | 10 | 1 | 2 | 14 | 8 | 9 | 8 | 3 | 5 | 3 | 6 | 7 | 5 | 5 | 6 | 10 | 14 | 2 | 5 | 3 | 0 | 3 | 4 |
Unique Cell Count | 148 | 132 | 91 | 103 | 160 | 159 | 224 | 212 | 165 | 141 | 171 | 207 | 76 | 80 | 87 | 263 | 201 | 190 | 203 | 201 | 142 | 54 | 147 | 184 |
Labelled Cell Count | 188 | 168 | 118 | 134 | 200 | 197 | 274 | 262 | 207 | 172 | 223 | 260 | 101 | 101 | 105 | 349 | 272 | 233 | 203 | 201 | 142 | 54 | 147 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.5 | 26.2 | 21.9 | 27.0 | 30.2 | 25.9 | 26.5 | 28.4 | 22.8 | 23.7 | 22.2 | 23.6 | 29.6 | 30.0 | 26.5 | 23.0 | 25.5 | 24.1 | – | 28.3 | 31.5 |
Std Deviation (1e-4) | 9.8 | 11.9 | 13.6 | 16.9 | 19.8 | 15.6 | 20.9 | 21.9 | 15.3 | 18.7 | 18.7 | 18.0 | 12.9 | 13.0 | 9.0 | 12.6 | 13.5 | 12.0 | – | 15.3 | 19.0 |
Intensity Change (Log2) | – | – | – | 0.3 | 0.47 | 0.25 | 0.28 | 0.38 | 0.06 | 0.12 | 0.02 | 0.11 | 0.44 | 0.46 | 0.28 | 0.07 | 0.22 | 0.14 | – | 0.37 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -3.0 | -2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | 0.3 | 1.0 | 0.6 | -0.5 | -0.1 |
Mitochondria | 0.6 | 0.3 | 3.5 | 2.2 | 2.3 | 3.0 | 2.9 | 4.5 | 2.6 | 5.3 | 2.9 | 2.8 | -2.7 | -1.9 | -3.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.4 | 4.2 | 3.7 | 4.1 | 4.9 | 4.5 | 3.9 | 4.2 | 4.3 | -0.6 | 1.0 | -2.1 | -0.6 | 0.2 | 0.5 |
Peroxisomes | -1.7 | -3.2 | -5.4 | -4.7 | -5.3 | -4.5 | -5.1 | -6.8 | -5.7 | -3.4 | -4.6 | -1.7 | 1.0 | 0.7 | -1.4 |
SpindlePole | 0.2 | -4.1 | -4.5 | -5.2 | -4.9 | -4.4 | -4.1 | -4.5 | -3.9 | -4.0 | -2.2 | -4.0 | 0.9 | 0.9 | 1.4 |
Vacuole | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.4339 | 44.629 | 32.7955 | 27.7047 | 31.8545 | 32.4343 | 21.0652 | 44.5181 | 30.2105 | 24.4996 | 29.4591 | 33.5947 | 30.3029 | 55.1425 | 40.9336 | 31.2894 | 42.6744 | 43.5286 |
Actin | 0.0058 | 0.0111 | 0.0071 | 0.0017 | 0.0001 | 0.0008 | 0.0189 | 0.0029 | 0.0008 | 0.0307 | 0.0005 | 0.0003 | 0.0116 | 0.0023 | 0.0087 | 0.0018 | 0.001 | 0.0018 |
Bud | 0.0014 | 0.0084 | 0.002 | 0.0002 | 0.0001 | 0.0029 | 0.0005 | 0.0017 | 0.0073 | 0.0002 | 0.0002 | 0.0005 | 0.0009 | 0.0017 | 0.0019 | 0.0004 | 0.0076 | 0.0002 |
Bud Neck | 0.0007 | 0.0082 | 0.0096 | 0.001 | 0.0264 | 0.0026 | 0.0007 | 0.0026 | 0.003 | 0.0011 | 0.002 | 0.0101 | 0.0017 | 0.0043 | 0.018 | 0.0015 | 0.0116 | 0.0016 |
Bud Periphery | 0.0007 | 0.0033 | 0.0012 | 0.0003 | 0.0001 | 0.0046 | 0.0003 | 0.0011 | 0.0013 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.001 | 0.0008 | 0.0006 | 0.004 | 0.0001 |
Bud Site | 0.0075 | 0.0686 | 0.0307 | 0.0015 | 0.0008 | 0.0027 | 0.0039 | 0.0132 | 0.0144 | 0.0015 | 0.001 | 0.0016 | 0.0124 | 0.0297 | 0.0254 | 0.0016 | 0.0266 | 0.001 |
Cell Periphery | 0.0009 | 0.0093 | 0.004 | 0.0019 | 0.0006 | 0.0042 | 0.0005 | 0.0027 | 0.0016 | 0.0006 | 0.001 | 0.0012 | 0.0009 | 0.0029 | 0.0021 | 0.0071 | 0.0038 | 0.0014 |
Cytoplasm | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0016 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasmic Foci | 0.5271 | 0.4243 | 0.5289 | 0.5173 | 0.6217 | 0.6408 | 0.2953 | 0.3874 | 0.4083 | 0.2703 | 0.4337 | 0.46 | 0.4557 | 0.4754 | 0.4578 | 0.1386 | 0.5151 | 0.5526 |
Eisosomes | 0.0005 | 0.0008 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0004 | 0.0005 | 0.001 | 0.0005 | 0.001 | 0.0007 | 0.0008 | 0.0056 | 0.0012 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0011 | 0.0018 | 0.001 | 0.0012 | 0.0239 | 0.0004 | 0.0121 | 0.0002 | 0.0004 | 0.0018 | 0.0036 | 0.0002 | 0.0017 | 0.0006 | 0.0004 | 0.0034 | 0.0002 | 0.0008 |
Golgi | 0.0021 | 0.0069 | 0.0063 | 0.0038 | 0.0024 | 0.0006 | 0.0088 | 0.0007 | 0.0012 | 0.0078 | 0.0046 | 0.0009 | 0.0036 | 0.0011 | 0.0012 | 0.0017 | 0.0008 | 0.0017 |
Lipid Particles | 0.0876 | 0.0604 | 0.057 | 0.1338 | 0.0681 | 0.0526 | 0.1152 | 0.0539 | 0.0868 | 0.1512 | 0.1456 | 0.0996 | 0.0994 | 0.0616 | 0.0577 | 0.0794 | 0.0769 | 0.0853 |
Mitochondria | 0.1928 | 0.1605 | 0.1548 | 0.1684 | 0.1548 | 0.1275 | 0.3283 | 0.3085 | 0.22 | 0.3318 | 0.2608 | 0.2098 | 0.2359 | 0.139 | 0.2054 | 0.4448 | 0.1274 | 0.1293 |
None | 0.0008 | 0.001 | 0.0005 | 0.0002 | 0.0004 | 0.0004 | 0.0037 | 0.0002 | 0.0017 | 0.0001 | 0.0016 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0039 | 0.0003 | 0.0003 | 0 | 0.0007 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0009 | 0.0003 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0682 | 0.1119 | 0.1069 | 0.0706 | 0.0532 | 0.0755 | 0.0539 | 0.1163 | 0.1063 | 0.0477 | 0.0786 | 0.11 | 0.0527 | 0.19 | 0.1205 | 0.1849 | 0.1005 | 0.0996 |
Nucleus | 0.0017 | 0.006 | 0.0042 | 0.0014 | 0.0012 | 0.0023 | 0.0101 | 0.0043 | 0.0036 | 0.0011 | 0.0021 | 0.0021 | 0.0018 | 0.0099 | 0.0033 | 0.004 | 0.005 | 0.0023 |
Peroxisomes | 0.0909 | 0.0585 | 0.0699 | 0.077 | 0.0347 | 0.0633 | 0.1271 | 0.0918 | 0.1244 | 0.1488 | 0.0568 | 0.0984 | 0.1059 | 0.0557 | 0.0835 | 0.0852 | 0.1126 | 0.1106 |
Punctate Nuclear | 0.0011 | 0.0155 | 0.0025 | 0.0021 | 0.0016 | 0.004 | 0.0127 | 0.004 | 0.0095 | 0.0008 | 0.0024 | 0.0006 | 0.009 | 0.0059 | 0.0032 | 0.0002 | 0.0031 | 0.0083 |
Vacuole | 0.0019 | 0.0178 | 0.0052 | 0.005 | 0.0047 | 0.0064 | 0.0012 | 0.0051 | 0.0026 | 0.0012 | 0.0024 | 0.0015 | 0.0012 | 0.006 | 0.0022 | 0.0258 | 0.002 | 0.0015 |
Vacuole Periphery | 0.0031 | 0.0248 | 0.0069 | 0.0122 | 0.0039 | 0.0078 | 0.0024 | 0.0026 | 0.0057 | 0.0019 | 0.0023 | 0.0016 | 0.0024 | 0.0115 | 0.0018 | 0.0169 | 0.0004 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 138.9614 | 154.9938 | 145.8793 | 107.8645 | 116.9449 | 157.997 | 145.7672 | 129.4923 | 123.8207 | 127.8307 |
Translational Efficiency | 0.9772 | 0.9334 | 0.8218 | 1.0664 | 0.9376 | 0.8116 | 0.9594 | 0.8176 | 0.843 | 0.8175 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
741 | 540 | 223 | 691 | 188 | 717 | 207 | 142 | 929 | 1257 | 430 | 833 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1227.74 | 1872.09 | 2487.66 | 1948.10 | 2064.26 | 2105.07 | 2165.05 | 2101.33 | 1397.02 | 2004.98 | 2332.36 | 1974.22 |
Standard Deviation | 321.35 | 639.54 | 628.84 | 593.59 | 549.95 | 703.24 | 641.04 | 610.84 | 506.48 | 686.37 | 654.89 | 599.35 |
Intensity Change Log 2 | — | 0.608645 | 1.018784 | 0.666063 | — | 0.028244 | 0.068776 | 0.025678 | — | 0.272774 | 0.499107 | 0.298755 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.029282 | 0.020337 | 0.007377 | 0.027347 | 0.009659 | 0.009167 | 0.006250 | 0.015324 | 0.025311 | 0.013966 | 0.006834 | 0.025297 |
Bud Neck | 0.005516 | 0.013374 | 0.025187 | 0.022874 | 0.012128 | 0.013643 | 0.017799 | 0.020209 | 0.006854 | 0.013527 | 0.021631 | 0.022420 |
Bud Site | 0.008237 | 0.022518 | 0.021015 | 0.065672 | 0.013941 | 0.017146 | 0.019601 | 0.062151 | 0.009391 | 0.019454 | 0.020334 | 0.065072 |
Cell Periphery | 0.000174 | 0.000322 | 0.000255 | 0.000515 | 0.000209 | 0.000246 | 0.000172 | 0.000473 | 0.000181 | 0.000279 | 0.000215 | 0.000508 |
Cytoplasm | 0.003367 | 0.004814 | 0.001808 | 0.009709 | 0.002544 | 0.005167 | 0.002378 | 0.011783 | 0.003200 | 0.005016 | 0.002082 | 0.010063 |
Cytoplasmic Foci | 0.199573* | 0.338756* | 0.482317* | 0.348107* | 0.559376* | 0.340188* | 0.543752* | 0.276463* | 0.272386* | 0.339573* | 0.511892* | 0.335894* |
Eisosomes | 0.005344 | 0.006603 | 0.001274 | 0.001085 | 0.002352 | 0.003148 | 0.000919 | 0.001135 | 0.004738 | 0.004632 | 0.001103 | 0.001093 |
Endoplasmic Reticulum | 0.000526 | 0.000366 | 0.000386 | 0.000744 | 0.000405 | 0.000386 | 0.000417 | 0.000491 | 0.000502 | 0.000377 | 0.000401 | 0.000701 |
Endosome | 0.003589 | 0.003107 | 0.004914 | 0.008218 | 0.005076 | 0.004964 | 0.005427 | 0.011439 | 0.003890 | 0.004166 | 0.005161 | 0.008767 |
Golgi | 0.041972 | 0.018905 | 0.010419 | 0.037825 | 0.021698 | 0.020822 | 0.017070 | 0.053085 | 0.037869 | 0.019998 | 0.013621 | 0.040427 |
Lipid Particles | 0.036797 | 0.017963 | 0.025682 | 0.022273 | 0.012967 | 0.033612 | 0.019024 | 0.017775 | 0.031975 | 0.026890 | 0.022477 | 0.021506 |
Mitochondria | 0.263623* | 0.148490* | 0.066076 | 0.187690* | 0.110315* | 0.100458* | 0.077678 | 0.287343* | 0.232598* | 0.121092* | 0.071661 | 0.204677* |
Mitotic Spindle | 0.005965 | 0.011259 | 0.097762 | 0.052125 | 0.015004 | 0.031129 | 0.068636 | 0.050101 | 0.007795 | 0.022593 | 0.083741 | 0.051780 |
None | 0.001479 | 0.001066 | 0.000519 | 0.000893 | 0.000503 | 0.000797 | 0.000428 | 0.001204 | 0.001281 | 0.000912 | 0.000475 | 0.000946 |
Nuclear Periphery | 0.000256 | 0.000273 | 0.004143 | 0.001285 | 0.000472 | 0.000621 | 0.004102 | 0.003935 | 0.000300 | 0.000471 | 0.004123 | 0.001737 |
Nuclear Periphery Foci | 0.000509 | 0.000812 | 0.003955 | 0.000878 | 0.001677 | 0.001499 | 0.003468 | 0.001933 | 0.000746 | 0.001204 | 0.003721 | 0.001057 |
Nucleolus | 0.002180 | 0.009588 | 0.008246 | 0.003962 | 0.012142 | 0.007269 | 0.007379 | 0.006821 | 0.004196 | 0.008265 | 0.007828 | 0.004449 |
Nucleus | 0.001209 | 0.003853 | 0.001014 | 0.001824 | 0.001700 | 0.001988 | 0.001318 | 0.000709 | 0.001309 | 0.002789 | 0.001160 | 0.001634 |
Peroxisomes | 0.385363* | 0.369249* | 0.207103* | 0.189423* | 0.200505* | 0.394909* | 0.177474* | 0.136011* | 0.347954* | 0.383885* | 0.192840* | 0.180318* |
Vacuole | 0.003488 | 0.006483 | 0.019864 | 0.009919 | 0.012057 | 0.009566 | 0.018539 | 0.018704 | 0.005222 | 0.008241 | 0.019226 | 0.011417 |
Vacuole Periphery | 0.001552 | 0.001863 | 0.010683 | 0.007631 | 0.005268 | 0.003274 | 0.008169 | 0.022913 | 0.002304 | 0.002668 | 0.009473 | 0.010236 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.88 | 5.79 | 0.52 | -1.32 | -4.92 | 0.12 | 0.86 | -1.18 | -1.87 | -2.65 | 3.54 | 6.13 | 0.12 | -3.21 | -5.63 |
Bud Neck | -5.46 | -6.86 | -11.12 | -5.03 | 0.81 | -0.82 | -2.36 | -2.95 | -2.61 | -0.74 | -6.48 | -8.04 | -11.27 | -6.04 | -0.27 |
Bud Site | -5.22 | -4.53 | -11.94 | -8.16 | -8.13 | -1.37 | -2.41 | -4.75 | -4.43 | -4.14 | -5.83 | -5.92 | -12.82 | -10.21 | -9.71 |
Cell Periphery | -1.61 | -1.72 | -6.46 | -1.99 | -3.84 | -0.86 | 0.67 | -3.49 | -3.05 | -4.07 | -2.18 | -1.32 | -7.15 | -3.92 | -5.73 |
Cytoplasm | -2.87 | 4.11 | -5.74 | -4.22 | -6.92 | -2.84 | -0.44 | -2.09 | -1.51 | -1.95 | -3.88 | 1.47 | -5.81 | -4.15 | -6.07 |
Cytoplasmic Foci | -8.53 | -11.37 | -9.90 | -0.84 | 4.92 | 8.33 | 0.44 | 8.19 | 2.30 | 7.98 | -5.10 | -12.57 | -4.58 | -0.10 | 8.73 |
Eisosomes | -1.37 | 9.00 | 9.90 | 6.93 | 1.15 | -1.76 | 4.13 | 3.02 | 5.22 | -0.84 | 0.24 | 10.08 | 10.29 | 9.02 | 0.17 |
Endoplasmic Reticulum | 2.54 | 1.47 | -0.73 | -1.31 | -1.20 | 0.17 | -0.09 | -0.62 | -0.92 | -0.56 | 2.28 | 1.40 | -0.80 | -1.34 | -1.22 |
Endosome | 0.69 | -1.27 | -4.99 | -5.12 | -2.63 | -0.03 | -0.18 | -2.58 | -2.73 | -2.42 | -0.53 | -1.42 | -5.53 | -4.84 | -3.30 |
Golgi | 5.30 | 7.95 | 0.94 | -3.76 | -5.96 | 0.16 | 0.80 | -2.55 | -2.75 | -2.98 | 5.28 | 6.73 | -0.37 | -4.66 | -5.93 |
Lipid Particles | 4.36 | 1.53 | 3.52 | -1.06 | 0.49 | -4.16 | -1.21 | -0.79 | 2.45 | 0.18 | 1.38 | 2.03 | 2.99 | 1.64 | 0.23 |
Mitochondria | 6.59 | 11.47 | 4.65 | -2.17 | -7.36 | 0.47 | 1.44 | -4.91 | -5.82 | -6.17 | 8.52 | 11.28 | 2.03 | -6.25 | -9.25 |
Mitotic Spindle | -2.59 | -6.31 | -8.29 | -7.06 | 2.92 | -2.48 | -4.50 | -2.69 | -1.50 | 1.14 | -5.26 | -8.20 | -8.55 | -5.24 | 3.03 |
None | 2.32 | 5.60 | 3.29 | 1.30 | -2.78 | -2.30 | 0.74 | -4.45 | -2.15 | -4.93 | 2.55 | 6.09 | 2.32 | -0.16 | -4.45 |
Nuclear Periphery | -0.18 | -2.47 | -4.05 | -4.08 | 1.79 | -0.77 | -2.51 | -2.30 | -2.21 | 0.07 | -1.92 | -3.57 | -4.31 | -3.81 | 2.13 |
Nuclear Periphery Foci | -1.77 | -1.85 | -2.40 | -0.35 | 1.66 | 0.31 | -1.53 | -0.32 | -0.57 | 1.22 | -2.30 | -2.70 | -1.66 | 0.75 | 2.41 |
Nucleolus | -3.73 | -2.06 | -2.54 | 2.68 | 1.42 | 1.43 | 1.27 | 1.05 | 0.11 | 0.15 | -3.17 | -1.94 | -0.22 | 2.78 | 1.75 |
Nucleus | -1.94 | 0.35 | -1.39 | 1.32 | -2.07 | -0.16 | 0.53 | 1.44 | 2.51 | 1.50 | -2.04 | 0.27 | -0.94 | 1.36 | -1.41 |
Peroxisomes | 0.90 | 8.35 | 12.84 | 10.96 | 0.93 | -9.25 | 1.10 | 2.61 | 11.80 | 1.72 | -2.48 | 9.95 | 12.52 | 16.54 | 0.92 |
Vacuole | -2.10 | -3.81 | -4.23 | -2.74 | 2.35 | 1.03 | -1.53 | -1.55 | -2.31 | -0.02 | -2.47 | -4.70 | -4.25 | -2.54 | 2.63 |
Vacuole Periphery | -0.57 | -3.04 | -5.24 | -5.25 | 0.99 | 1.01 | -0.98 | -2.87 | -3.33 | -2.34 | -0.62 | -3.64 | -5.47 | -5.46 | -0.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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