Standard name
Human Ortholog
Description Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.1 0.08 0.08 0.08 0.08 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.12 0.05 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0.05 0
Golgi 0 0.06 0.11 0 0 0 0 0 0 0 0 0 0.05 0.12 0.16 0.13 0.09 0.11 0.07 0.1 0.11 0.12 0.11 0.14
Mitochondria 0.56 0.55 0.3 0.34 0.31 0.52 0.43 0.44 0.49 0.49 0.59 0.46 0.71 0.51 0.51 0.16 0.19 0.13 0.27 0.37 0.22 0.16 0.13 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.3 0.22 0.38 0.49 0.45 0.47 0.52 0.51 0.48 0.49 0.49 0.18 0.29 0.1 0.19 0.23 0.25 0.06 0.07 0.06 0.11 0.16 0.12
Peroxisomes 0.19 0.14 0.33 0.22 0.16 0.07 0.11 0.08 0.1 0.07 0 0.07 0.11 0.05 0.22 0.39 0.37 0.25 0.18 0.11 0.21 0.19 0.14 0.2
SpindlePole 0.08 0.08 0.24 0.25 0.06 0.05 0 0.05 0.05 0.06 0.05 0.08 0 0.11 0 0.29 0.29 0.32 0.13 0.07 0.13 0.14 0.17 0.13
Vac/Vac Membrane 0 0.08 0 0 0.09 0.05 0 0 0 0 0 0 0.09 0.06 0.06 0 0.05 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 1 0 1 0 1 0 0 1 2 1 5 2 3 1 2 21 15 11 4 11 10
Bud 2 0 0 2 2 3 1 1 3 1 5 4 0 0 2 0 0 0 4 4 4 0 2 2
Bud Neck 1 2 0 1 1 1 0 0 0 3 0 5 0 0 0 2 3 0 9 13 9 6 7 12
Bud Site 4 0 0 0 0 1 1 1 0 0 0 0 1 0 0 4 2 8
Cell Periphery 0 1 1 0 0 1 1 0 0 0 0 0 2 0 2 7 1 1 0 0 0 0 0 0
Cytoplasm 2 1 3 2 8 2 10 8 5 4 5 2 5 4 3 9 3 4 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 3 6 1 0 0 0 0 0 1
Endosome 3 1 4 1 5 3 5 2 2 1 2 3 1 0 1 6 6 3 14 10 7 2 7 6
Golgi 0 8 10 1 6 2 2 2 0 1 0 1 4 10 14 35 18 20 13 19 15 6 16 25
Mitochondria 83 72 27 35 50 83 96 93 81 69 101 95 54 41 44 43 39 24 54 74 31 8 19 31
Nucleus 1 1 0 1 1 0 4 5 1 3 1 4 0 0 1 2 3 1 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 1 5 1 2 0 7 6 1 0 0 1 0 0 0 1 0 0 0 0
Nucleolus 49 40 20 39 78 72 105 111 84 67 83 101 14 23 9 50 46 47 12 14 8 5 23 21
Peroxisomes 28 19 30 23 25 11 24 18 17 10 5 15 8 4 19 102 75 47 36 22 29 9 20 37
SpindlePole 12 11 22 26 10 8 10 11 9 8 9 16 2 9 3 76 59 61 27 14 18 7 25 23
Vac/Vac Membrane 2 10 1 2 14 8 9 8 3 5 3 6 7 5 5 6 10 14 2 5 3 0 3 4
Unique Cell Count 148 132 91 103 160 159 224 212 165 141 171 207 76 80 87 263 201 190 203 201 142 54 147 184
Labelled Cell Count 188 168 118 134 200 197 274 262 207 172 223 260 101 101 105 349 272 233 203 201 142 54 147 184


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.5 26.2 21.9 27.0 30.2 25.9 26.5 28.4 22.8 23.7 22.2 23.6 29.6 30.0 26.5 23.0 25.5 24.1 28.3 31.5
Std Deviation (1e-4) 9.8 11.9 13.6 16.9 19.8 15.6 20.9 21.9 15.3 18.7 18.7 18.0 12.9 13.0 9.0 12.6 13.5 12.0 15.3 19.0
Intensity Change (Log2) 0.3 0.47 0.25 0.28 0.38 0.06 0.12 0.02 0.11 0.44 0.46 0.28 0.07 0.22 0.14 0.37 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.0 -2.3 0 0 0 0 0 0 0 -1.3 0.3 1.0 0.6 -0.5 -0.1
Mitochondria 0.6 0.3 3.5 2.2 2.3 3.0 2.9 4.5 2.6 5.3 2.9 2.8 -2.7 -1.9 -3.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.4 4.2 3.7 4.1 4.9 4.5 3.9 4.2 4.3 -0.6 1.0 -2.1 -0.6 0.2 0.5
Peroxisomes -1.7 -3.2 -5.4 -4.7 -5.3 -4.5 -5.1 -6.8 -5.7 -3.4 -4.6 -1.7 1.0 0.7 -1.4
SpindlePole 0.2 -4.1 -4.5 -5.2 -4.9 -4.4 -4.1 -4.5 -3.9 -4.0 -2.2 -4.0 0.9 0.9 1.4
Vacuole 0 2.5 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.4339 44.629 32.7955 27.7047 31.8545 32.4343 21.0652 44.5181 30.2105 24.4996 29.4591 33.5947 30.3029 55.1425 40.9336 31.2894 42.6744 43.5286
Actin 0.0058 0.0111 0.0071 0.0017 0.0001 0.0008 0.0189 0.0029 0.0008 0.0307 0.0005 0.0003 0.0116 0.0023 0.0087 0.0018 0.001 0.0018
Bud 0.0014 0.0084 0.002 0.0002 0.0001 0.0029 0.0005 0.0017 0.0073 0.0002 0.0002 0.0005 0.0009 0.0017 0.0019 0.0004 0.0076 0.0002
Bud Neck 0.0007 0.0082 0.0096 0.001 0.0264 0.0026 0.0007 0.0026 0.003 0.0011 0.002 0.0101 0.0017 0.0043 0.018 0.0015 0.0116 0.0016
Bud Periphery 0.0007 0.0033 0.0012 0.0003 0.0001 0.0046 0.0003 0.0011 0.0013 0.0003 0.0002 0.0002 0.0004 0.001 0.0008 0.0006 0.004 0.0001
Bud Site 0.0075 0.0686 0.0307 0.0015 0.0008 0.0027 0.0039 0.0132 0.0144 0.0015 0.001 0.0016 0.0124 0.0297 0.0254 0.0016 0.0266 0.001
Cell Periphery 0.0009 0.0093 0.004 0.0019 0.0006 0.0042 0.0005 0.0027 0.0016 0.0006 0.001 0.0012 0.0009 0.0029 0.0021 0.0071 0.0038 0.0014
Cytoplasm 0.0002 0.0005 0.0003 0.0001 0.0002 0.0003 0.0016 0.0001 0.0003 0 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001
Cytoplasmic Foci 0.5271 0.4243 0.5289 0.5173 0.6217 0.6408 0.2953 0.3874 0.4083 0.2703 0.4337 0.46 0.4557 0.4754 0.4578 0.1386 0.5151 0.5526
Eisosomes 0.0005 0.0008 0.0007 0.0003 0.0001 0.0003 0.0009 0.0004 0.0005 0.001 0.0005 0.001 0.0007 0.0008 0.0056 0.0012 0.0004 0.0002
Endoplasmic Reticulum 0.0002 0 0 0 0.0003 0 0.0005 0 0 0.0001 0 0 0.0001 0 0 0 0 0
Endosome 0.0011 0.0018 0.001 0.0012 0.0239 0.0004 0.0121 0.0002 0.0004 0.0018 0.0036 0.0002 0.0017 0.0006 0.0004 0.0034 0.0002 0.0008
Golgi 0.0021 0.0069 0.0063 0.0038 0.0024 0.0006 0.0088 0.0007 0.0012 0.0078 0.0046 0.0009 0.0036 0.0011 0.0012 0.0017 0.0008 0.0017
Lipid Particles 0.0876 0.0604 0.057 0.1338 0.0681 0.0526 0.1152 0.0539 0.0868 0.1512 0.1456 0.0996 0.0994 0.0616 0.0577 0.0794 0.0769 0.0853
Mitochondria 0.1928 0.1605 0.1548 0.1684 0.1548 0.1275 0.3283 0.3085 0.22 0.3318 0.2608 0.2098 0.2359 0.139 0.2054 0.4448 0.1274 0.1293
None 0.0008 0.001 0.0005 0.0002 0.0004 0.0004 0.0037 0.0002 0.0017 0.0001 0.0016 0.0002 0.0007 0.0002 0.0004 0.0005 0.0004 0.0001
Nuclear Periphery 0.0039 0.0003 0.0003 0 0.0007 0.0001 0.0012 0 0.0001 0.0001 0 0 0.0009 0.0003 0 0 0 0
Nucleolus 0.0682 0.1119 0.1069 0.0706 0.0532 0.0755 0.0539 0.1163 0.1063 0.0477 0.0786 0.11 0.0527 0.19 0.1205 0.1849 0.1005 0.0996
Nucleus 0.0017 0.006 0.0042 0.0014 0.0012 0.0023 0.0101 0.0043 0.0036 0.0011 0.0021 0.0021 0.0018 0.0099 0.0033 0.004 0.005 0.0023
Peroxisomes 0.0909 0.0585 0.0699 0.077 0.0347 0.0633 0.1271 0.0918 0.1244 0.1488 0.0568 0.0984 0.1059 0.0557 0.0835 0.0852 0.1126 0.1106
Punctate Nuclear 0.0011 0.0155 0.0025 0.0021 0.0016 0.004 0.0127 0.004 0.0095 0.0008 0.0024 0.0006 0.009 0.0059 0.0032 0.0002 0.0031 0.0083
Vacuole 0.0019 0.0178 0.0052 0.005 0.0047 0.0064 0.0012 0.0051 0.0026 0.0012 0.0024 0.0015 0.0012 0.006 0.0022 0.0258 0.002 0.0015
Vacuole Periphery 0.0031 0.0248 0.0069 0.0122 0.0039 0.0078 0.0024 0.0026 0.0057 0.0019 0.0023 0.0016 0.0024 0.0115 0.0018 0.0169 0.0004 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 138.9614 154.9938 145.8793 107.8645 116.9449 157.997 145.7672 129.4923 123.8207 127.8307
Translational Efficiency 0.9772 0.9334 0.8218 1.0664 0.9376 0.8116 0.9594 0.8176 0.843 0.8175

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency