Standard name
Description Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.1 0.18 0 0 0 0 0.05 0.06 0.07 0 0 0.08 0.1 0.29 0.24 0.44 0.57 0.36 0 0 0 0 0 0
Cytoplasm 0.97 0.88 1.0 1.0 0.98 0.98 0.97 0.96 0.92 0.94 0.94 0.91 0.92 0.81 0.8 0.73 0.61 0.77 0.96 0.96 0.98 0.95 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 18 47 3 0 3 4 6 9 8 5 5 15 14 33 35 92 88 50 2 4 0 0 0 2
Cytoplasm 167 232 107 88 100 124 120 153 101 116 133 162 123 92 114 154 95 106 197 326 114 119 190 179
Endoplasmic Reticulum 0 1 0 0 0 0 2 2 5 4 5 7 0 0 0 1 6 7 0 0 0 1 4 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 3 1
Unique Cell Count 173 265 107 88 102 127 124 160 110 123 142 178 134 114 143 210 155 137 206 339 117 126 208 191
Labelled Cell Count 185 281 110 88 103 129 129 164 116 127 144 186 137 125 149 247 190 163 206 339 117 126 208 191


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 456.2 470.6 330.6 291.3 284.5 227.7 206.3 175.4 158.2 128.5 130.5 135.3 485.3 505.9 487.9 627.9 637.1 589.7 375.9 402.8 423.1
Std Deviation (1e-4) 91.5 88.5 80.2 94.2 77.9 72.6 63.4 60.6 50.9 47.0 42.0 39.5 86.4 132.5 108.3 119.5 121.8 127.3 99.2 113.1 134.5
Intensity Change (Log2) – – – -0.18 -0.22 -0.54 -0.68 -0.91 -1.06 -1.36 -1.34 -1.29 0.55 0.61 0.56 0.93 0.95 0.83 0.19 0.29 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.5 0 0 1.9 2.3 5.3 4.7 7.5 9.0 6.3
Cytoplasm 0 -1.5 -1.6 -1.9 -2.2 -3.0 -2.5 -2.7 -3.2 -3.0 -4.8 -5.0 -5.9 -7.3 -5.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 530.3302 807.6401 594.5139 550.3847 618.4806 652.8472 445.16 984.2512 563.7692 401.5141 447.7044 736.0263 688.4178 987.9898 729.2842 807.5749 670.1007 756.4286
Actin 0.013 0.0002 0.0091 0.0166 0.0003 0.0006 0.0017 0.0002 0.004 0.0016 0.0456 0.0009 0.0008 0.0002 0.0004 0.0002 0.0004 0.0008
Bud 0.0008 0.0008 0.0049 0.0004 0.0002 0.0008 0.0036 0.0002 0.0031 0.0053 0.0394 0.0004 0.0042 0.0009 0.0026 0.0004 0.0008 0.001
Bud Neck 0.0006 0.0002 0.0013 0.0006 0.0016 0.0008 0.0008 0.0001 0.0005 0.0011 0.0007 0.0008 0.0004 0.0001 0.0003 0.0003 0.001 0.0006
Bud Periphery 0.0005 0.0002 0.003 0.0002 0 0.0001 0.0018 0 0.0044 0.0021 0.0289 0.0001 0.0017 0.0002 0.001 0.0002 0.0002 0.0002
Bud Site 0.0007 0.0006 0.0029 0.002 0 0.0001 0.0043 0.0001 0.0016 0.0063 0.0182 0 0.0011 0.0001 0.0005 0.0002 0.0002 0.0001
Cell Periphery 0.0042 0.0033 0.0017 0.0001 0.0005 0.0004 0.0062 0.0003 0.004 0.0125 0.0037 0.0003 0.0029 0.0005 0.0014 0.0007 0.0024 0.0004
Cytoplasm 0.7424 0.9424 0.8098 0.7246 0.9732 0.8615 0.5912 0.98 0.6954 0.6543 0.5514 0.9397 0.8279 0.9706 0.8337 0.8742 0.8069 0.9559
Cytoplasmic Foci 0.0302 0.0001 0.0043 0.0158 0.0002 0.0007 0.0328 0.0002 0.0148 0.0517 0.0201 0.0006 0.0219 0.0001 0.0143 0.0038 0.0307 0.001
Eisosomes 0.0017 0 0.0011 0.0001 0 0.0003 0.0008 0 0.0053 0.0054 0.0035 0.0001 0.0009 0 0.0006 0.0006 0.0002 0.0001
Endoplasmic Reticulum 0.0072 0.0001 0.0045 0.0001 0.0002 0.0001 0.0022 0 0.0021 0.0015 0.0017 0.0001 0.0009 0.0001 0.0005 0.0002 0.0005 0.0003
Endosome 0.0063 0 0.0161 0.004 0 0.0001 0.0116 0 0.005 0.0002 0.0007 0.0002 0.0054 0 0.0048 0 0.0001 0.0001
Golgi 0.0055 0 0.0033 0.0082 0 0 0.0008 0 0.001 0.0002 0.0007 0 0.0006 0 0.0002 0 0 0
Lipid Particles 0.0059 0 0.0046 0.0274 0 0.0003 0.006 0 0.0052 0.0056 0.0013 0 0.0025 0 0.001 0.0003 0.0013 0.0002
Mitochondria 0.0155 0.0024 0.0114 0.0053 0.0003 0.0032 0.0284 0.0014 0.0515 0.0308 0.2104 0.0034 0.0221 0.001 0.014 0.0117 0.0081 0.0016
None 0.0192 0.0006 0.0229 0.0004 0.0002 0.0029 0.0288 0.0006 0.0215 0.1255 0.0376 0.0023 0.0205 0.0007 0.0311 0.0609 0.0131 0.0017
Nuclear Periphery 0.0136 0.0025 0.0141 0.0052 0.006 0.0098 0.0159 0.002 0.0132 0.0041 0.0014 0.0079 0.0086 0.0019 0.0041 0.0035 0.0094 0.0064
Nucleolus 0.0507 0.0032 0.0089 0.0002 0.0002 0.0302 0.111 0.0005 0.0493 0.0382 0.0053 0.0038 0.0283 0.0005 0.0489 0.0101 0.0183 0.0013
Nucleus 0.0656 0.0394 0.0536 0.0683 0.0161 0.0823 0.0904 0.0138 0.0362 0.0297 0.0126 0.036 0.0388 0.0213 0.0276 0.0299 0.0979 0.0261
Peroxisomes 0.0019 0.0001 0.0012 0.119 0 0.0001 0.0015 0 0.0015 0.0022 0.0092 0.0001 0.0011 0 0.0006 0.0003 0.0005 0.0001
Punctate Nuclear 0.007 0 0.0024 0.001 0.0001 0.0018 0.0079 0.0001 0.0368 0.0034 0.0007 0.0005 0.0042 0.0001 0.0077 0.0009 0.0023 0.0004
Vacuole 0.0052 0.0032 0.011 0.0004 0.0007 0.0018 0.0415 0.0002 0.0238 0.0154 0.0045 0.0012 0.0036 0.0013 0.0031 0.001 0.0039 0.0011
Vacuole Periphery 0.0024 0.0007 0.0079 0.0004 0.0004 0.0021 0.0107 0.0002 0.0198 0.0028 0.0023 0.0015 0.0015 0.0004 0.0017 0.0004 0.0017 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 493.036 221.7634 228.0939 405.3018 268.5502 339.3579 119.2547 144.9092 301.8031 252.1417
Translational Efficiency 1.5684 1.4905 1.4591 1.4629 1.6271 1.762 1.4347 1.0996 1.186 1.2546

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘