| Standard name | |
|---|---|
| Description | Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 1 | 7 | 0 | 1 | 3 |
| Bud | 2 | 0 | 0 | 3 | 4 | 4 | 17 | 9 | 9 | 9 | 10 | 2 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 13 |
| Bud Neck | 0 | 0 | 0 | 2 | 3 | 2 | 3 | 1 | 3 | 1 | 0 | 1 | 1 | 5 | 0 | – | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 7 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 8 | 1 | – | 6 | 1 | 0 | 0 | 4 | 0 | 0 | 2 |
| Cytoplasm | 101 | 124 | 252 | 213 | 300 | 284 | 340 | 347 | 258 | 252 | 139 | 174 | 253 | 421 | 450 | – | 130 | 89 | 82 | 105 | 144 | 108 | 224 | 313 |
| Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | – | 7 | 7 | 4 | 0 | 2 | 0 | 2 | 1 |
| Endosome | 0 | 0 | 1 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | – | 2 | 4 | 2 | 3 | 5 | 0 | 1 | 1 |
| Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
| Mitochondria | 38 | 8 | 2 | 27 | 20 | 112 | 200 | 139 | 137 | 191 | 172 | 183 | 0 | 1 | 1 | – | 3 | 3 | 29 | 13 | 55 | 2 | 6 | 9 |
| Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
| Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 1 | 0 | 3 | 2 | 3 | 2 | 7 | 27 | 11 | 10 | 6 | 9 | 0 | 0 | 0 | – | 1 | 3 | 3 | 2 | 5 | 0 | 1 | 8 |
| Vac/Vac Membrane | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 8 | 0 | 2 | 4 | 4 | 0 | 0 | 0 | – | 8 | 5 | 4 | 4 | 6 | 1 | 3 | 2 |
| Unique Cell Count | 124 | 127 | 253 | 228 | 315 | 328 | 463 | 448 | 345 | 382 | 252 | 304 | 257 | 423 | 455 | 141 | 101 | 140 | 136 | 253 | 120 | 248 | 374 | |
| Labelled Cell Count | 143 | 132 | 262 | 253 | 340 | 407 | 575 | 543 | 421 | 470 | 335 | 383 | 260 | 437 | 460 | 160 | 112 | 140 | 136 | 253 | 120 | 248 | 374 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.1 | 3.9 | 4.1 | 3.4 | 3.7 | 3.0 | 3.0 | 3.0 | 2.8 | 2.7 | 2.5 | 2.5 | 4.7 | 4.7 | 4.8 | 4.8 | 5.1 | 5.8 | 4.2 | 4.8 | 5.4 |
| Std Deviation (1e-4) | 0.6 | 0.9 | 4.5 | 1.5 | 1.7 | 1.2 | 1.6 | 1.8 | 1.4 | 1.2 | 1.3 | 1.2 | 1.0 | 1.2 | 1.2 | 1.3 | 1.5 | 2.6 | 1.3 | 1.2 | 1.4 |
| Intensity Change (Log2) | – | – | – | -0.25 | -0.15 | -0.45 | -0.46 | -0.43 | -0.55 | -0.59 | -0.69 | -0.7 | 0.22 | 0.2 | 0.22 | 0.23 | 0.33 | 0.51 | 0.04 | 0.24 | 0.4 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Rad51-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
