Standard name
Description DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.13 0.18 0.14 0.18 0.13 0.1 0.11 0.08 0.07 0.05 0.13 0.22 0.24 0.05 0.13 0.06 0.07 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.06 0 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.37 0.45 0.24 0.45 0.48 0.55 0.65 0.71 0.72 0.07 0.08 0.2 0.05 0 0 0.05 0 0 0.06
Nucleus 0.67 0.69 0.58 0.6 0.59 0.59 0.57 0.52 0.49 0.52 0.47 0.8 0.59 0.53 0.67 0.45 0.54 0.4 0.54 0.4 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.1 0.1 0.13 0.17 0.16 0.16 0.06 0 0.05 0.05 0.13 0.11 0.11 0.15 0.12 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.11 0.07 0.11 0.16
Vac/Vac Membrane 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.14 0.06 0.09 0.13 0.12 0.16 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 3 1 4 12 8 9 9 0 0 0 0 1 8 4 1 4 2
Bud Neck 0 0 0 1 0 1 2 3 1 1 1 0 0 0 0 0 1 1 2 1 3
Bud Site 0 0 0 0 2 9 9 13 14 15 13 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 33 37 45 29 50 44 48 62 30 24 19 24 49 59 1 26 20 22 6 7 7
Endoplasmic Reticulum 0 4 1 0 0 0 0 1 0 0 0 0 0 2 0 0 0 1 0 0 1
Endosome 9 7 4 1 10 2 4 4 1 1 0 3 6 2 1 11 15 19 5 17 19
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 1 1 0
Mitochondria 3 40 92 93 67 150 226 325 243 254 289 13 17 49 1 7 6 13 2 7 14
Nucleus 112 195 145 124 163 196 268 305 181 187 188 152 133 130 14 93 178 122 95 92 59
Nuclear Periphery 0 0 1 1 2 0 7 8 2 6 8 1 0 1 0 2 3 2 0 2 2
Nucleolus 6 8 4 5 13 32 46 78 64 56 66 11 5 12 1 27 34 34 26 26 30
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 6 2 13 1 9 5 21 16 8 5 4 3 4 3 0 16 22 32 11 24 36
Vac/Vac Membrane 8 22 5 4 9 8 15 9 9 1 9 3 27 15 3 12 28 38 20 36 37
Unique Cell Count 168 282 252 206 276 333 474 586 372 359 403 191 225 243 21 207 328 305 175 229 227
Labelled Cell Count 177 316 310 259 328 448 650 836 561 559 606 210 241 273 21 207 328 305 175 229 227


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.7 4.1 3.8 4.6 4.2 4.1 3.9 3.5 3.6 3.6 5.1 4.7 4.1 7.4 4.7 4.7 5.4
Std Deviation (1e-4) 0.9 0.9 1.2 1.0 1.6 1.4 1.4 1.3 1.3 1.3 1.4 1.1 1.0 1.1 1.5 1.0 1.3 1.6
Intensity Change (Log2) -0.1 0.15 0.03 -0.02 -0.08 -0.22 -0.18 -0.18 0.3 0.18 0.0 0.86 0.2 0.21 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole