| Standard name | |
|---|---|
| Description | DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
| Bud | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 12 | 8 | – | 9 | 9 | 0 | 0 | 0 | 0 | – | – | 1 | 8 | 4 | 1 | 4 | 2 |
| Bud Neck | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 1 | 2 | 1 | 3 |
| Bud Site | 0 | 0 | 0 | 0 | 2 | 9 | 9 | 13 | 14 | – | 15 | 13 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 33 | 37 | 45 | 29 | 50 | 44 | 48 | 62 | 30 | – | 24 | 19 | 24 | 49 | 59 | 1 | – | – | 26 | 20 | 22 | 6 | 7 | 7 |
| Endoplasmic Reticulum | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 1 |
| Endosome | 9 | 7 | 4 | 1 | 10 | 2 | 4 | 4 | 1 | – | 1 | 0 | 3 | 6 | 2 | 1 | – | – | 11 | 15 | 19 | 5 | 17 | 19 |
| Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 5 | 3 | 1 | 1 | 0 |
| Mitochondria | 3 | 40 | 92 | 93 | 67 | 150 | 226 | 325 | 243 | – | 254 | 289 | 13 | 17 | 49 | 1 | – | – | 7 | 6 | 13 | 2 | 7 | 14 |
| Nucleus | 112 | 195 | 145 | 124 | 163 | 196 | 268 | 305 | 181 | – | 187 | 188 | 152 | 133 | 130 | 14 | – | – | 93 | 178 | 122 | 95 | 92 | 59 |
| Nuclear Periphery | 0 | 0 | 1 | 1 | 2 | 0 | 7 | 8 | 2 | – | 6 | 8 | 1 | 0 | 1 | 0 | – | – | 2 | 3 | 2 | 0 | 2 | 2 |
| Nucleolus | 6 | 8 | 4 | 5 | 13 | 32 | 46 | 78 | 64 | – | 56 | 66 | 11 | 5 | 12 | 1 | – | – | 27 | 34 | 34 | 26 | 26 | 30 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 6 | 2 | 13 | 1 | 9 | 5 | 21 | 16 | 8 | – | 5 | 4 | 3 | 4 | 3 | 0 | – | – | 16 | 22 | 32 | 11 | 24 | 36 |
| Vac/Vac Membrane | 8 | 22 | 5 | 4 | 9 | 8 | 15 | 9 | 9 | – | 1 | 9 | 3 | 27 | 15 | 3 | – | – | 12 | 28 | 38 | 20 | 36 | 37 |
| Unique Cell Count | 168 | 282 | 252 | 206 | 276 | 333 | 474 | 586 | 372 | 359 | 403 | 191 | 225 | 243 | 21 | 207 | 328 | 305 | 175 | 229 | 227 | |||
| Labelled Cell Count | 177 | 316 | 310 | 259 | 328 | 448 | 650 | 836 | 561 | 559 | 606 | 210 | 241 | 273 | 21 | 207 | 328 | 305 | 175 | 229 | 227 | |||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 5.5 | 4.7 | 4.1 | 3.8 | 4.6 | 4.2 | 4.1 | 3.9 | 3.5 | – | 3.6 | 3.6 | 5.1 | 4.7 | 4.1 | 7.4 | – | – | 4.7 | 4.7 | 5.4 |
| Std Deviation (1e-4) | 0.9 | 0.9 | 1.2 | 1.0 | 1.6 | 1.4 | 1.4 | 1.3 | 1.3 | – | 1.3 | 1.4 | 1.1 | 1.0 | 1.1 | 1.5 | – | – | 1.0 | 1.3 | 1.6 |
| Intensity Change (Log2) | – | – | – | -0.1 | 0.15 | 0.03 | -0.02 | -0.08 | -0.22 | – | -0.18 | -0.18 | 0.3 | 0.18 | 0.0 | 0.86 | – | – | 0.2 | 0.21 | 0.39 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
