Standard name | |
---|---|
Human Ortholog | |
Description | Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 54 | 30 | 74 | 64 | 52 | 68 | 30 | 11 | – | 19 | 17 | 14 | 30 | 38 | 22 | 4 | 12 | 7 | 47 | 25 | 104 | 21 | 47 | 35 |
Bud | 10 | 8 | 47 | 32 | 53 | 75 | 87 | 74 | – | 62 | 61 | 65 | 14 | 14 | 2 | 0 | 3 | 0 | 17 | 20 | 14 | 22 | 53 | 35 |
Bud Neck | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 0 | – | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 28 | 21 | 22 | 18 | 31 | 22 |
Bud Site | 7 | 6 | 9 | 11 | 7 | 14 | 10 | 5 | – | 1 | 4 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 3 | 3 | 2 | 1 | 7 | 8 | – | 11 | 7 | 5 | 6 | 12 | 8 | 0 | 0 | 1 | 1 | 0 | 4 | 0 | 0 | 0 |
Cytoplasm | 83 | 72 | 133 | 202 | 320 | 452 | 586 | 617 | – | 595 | 452 | 551 | 60 | 72 | 101 | 32 | 34 | 21 | 6 | 10 | 7 | 3 | 5 | 5 |
Endoplasmic Reticulum | 57 | 37 | 70 | 45 | 29 | 51 | 28 | 19 | – | 20 | 5 | 12 | 36 | 41 | 44 | 3 | 2 | 2 | 84 | 43 | 93 | 8 | 20 | 28 |
Endosome | 14 | 2 | 17 | 6 | 49 | 28 | 39 | 24 | – | 35 | 5 | 8 | 1 | 7 | 11 | 4 | 5 | 1 | 18 | 6 | 40 | 7 | 14 | 14 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 3 | 10 | 1 | 13 | 1 | 1 | 4 |
Mitochondria | 1 | 2 | 26 | 11 | 13 | 62 | 124 | 121 | – | 107 | 133 | 163 | 1 | 0 | 0 | 5 | 6 | 2 | 4 | 3 | 24 | 8 | 14 | 8 |
Nucleus | 1 | 0 | 0 | 2 | 3 | 3 | 4 | 10 | – | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 1 | 5 | 2 | 4 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 10 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 18 | 10 | 11 | 24 | 23 | 9 | 14 | 4 | – | 3 | 3 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 3 | 2 | 1 |
SpindlePole | 3 | 2 | 7 | 8 | 13 | 13 | 19 | 13 | – | 10 | 2 | 9 | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 44 | 5 | 11 | 8 | 32 | 30 | 40 | 42 | – | 61 | 23 | 37 | 2 | 4 | 12 | 32 | 33 | 22 | 88 | 42 | 70 | 25 | 60 | 64 |
Unique Cell Count | 240 | 139 | 318 | 342 | 492 | 667 | 835 | 836 | 800 | 583 | 733 | 131 | 154 | 172 | 73 | 81 | 55 | 315 | 181 | 407 | 125 | 258 | 232 | |
Labelled Cell Count | 296 | 176 | 410 | 420 | 600 | 812 | 996 | 962 | 939 | 715 | 874 | 153 | 194 | 202 | 82 | 97 | 59 | 315 | 181 | 407 | 125 | 258 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.2 | 8.6 | 6.0 | 6.0 | 5.8 | 5.5 | 4.6 | 4.9 | – | 4.5 | 4.3 | 4.3 | 7.6 | 8.1 | 8.5 | 10.9 | 10.2 | 10.7 | 8.7 | 9.0 | 8.8 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.0 | 1.4 | 1.2 | 1.5 | 1.1 | 1.2 | – | 1.3 | 0.9 | 0.9 | 1.1 | 1.4 | 1.7 | 2.4 | 2.4 | 2.4 | 1.4 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.03 | -0.11 | -0.38 | -0.3 | – | -0.39 | -0.47 | -0.48 | 0.34 | 0.44 | 0.51 | 0.86 | 0.78 | 0.84 | 0.55 | 0.59 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -4.9 | -5.5 | 0 | 0 | – | 0 | 0 | 0 | -0.1 | 0.3 | -2.8 | -3.4 | -1.7 | -1.8 |
Bud | -2.1 | -1.7 | -1.6 | 0 | 0 | – | 0 | 0 | 0 | -1.2 | -1.7 | -4.8 | -3.5 | -2.7 | -3.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.3 | -1.4 | -0.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.4 | 6.5 | 7.8 | 8.9 | 10.2 | – | 10.3 | 10.7 | 10.4 | 0.8 | 1.0 | 3.6 | 0.3 | 0 | -0.5 |
Endoplasmic Reticulum | -3.0 | -6.8 | -6.4 | 0 | 0 | – | 0 | 0 | 0 | 1.2 | 1.1 | 0.9 | -3.5 | -4.1 | -3.2 |
Endosome | -2.5 | 2.3 | -0.8 | 0 | 0 | – | 0 | 0 | 0 | -2.3 | -0.4 | 0.5 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.8 | -3.6 | 0.6 | 0 | 0 | – | 0 | 0 | 0 | -3.0 | -3.7 | -3.9 | -0.4 | -0.2 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2.0 | 0.8 | -2.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.1 | 0.4 | -0.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.9 | 1.9 | 0.8 | 1.0 | 1.1 | – | 2.6 | 0.4 | 1.1 | 0 | 0 | 1.8 | 9.9 | 9.6 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0525 | 4.2213 | 4.1998 | 3.6234 | 3.4248 | 3.9813 | 4.8054 | 6.4902 | 6.1237 | 5.6107 | 6.0095 | 5.9961 | 2.7454 | 4.0586 | 3.8538 | 3.2662 | 3.3877 | 3.786 |
Actin | 0.072 | 0.0424 | 0.0692 | 0.0571 | 0.0694 | 0.0313 | 0.0813 | 0.0221 | 0.0714 | 0.0356 | 0.0302 | 0.0639 | 0.0542 | 0.0229 | 0.0518 | 0.0396 | 0.0088 | 0.0193 |
Bud | 0.0269 | 0.0265 | 0.1371 | 0.0522 | 0.0291 | 0.0086 | 0.0203 | 0.0188 | 0.0687 | 0.0105 | 0.0036 | 0.0037 | 0.0303 | 0.0165 | 0.1022 | 0.0216 | 0.0185 | 0.0025 |
Bud Neck | 0.0396 | 0.0088 | 0.0075 | 0.0117 | 0.0103 | 0.514 | 0.0332 | 0.0165 | 0.0097 | 0.003 | 0.007 | 0.5015 | 0.0315 | 0.0138 | 0.0069 | 0.0093 | 0.0343 | 0.5666 |
Bud Periphery | 0.057 | 0.0487 | 0.3031 | 0.1738 | 0.076 | 0.0186 | 0.0609 | 0.0346 | 0.205 | 0.1057 | 0.0128 | 0.008 | 0.0771 | 0.021 | 0.204 | 0.1 | 0.0486 | 0.0061 |
Bud Site | 0.1616 | 0.2373 | 0.0665 | 0.0273 | 0.0375 | 0.0083 | 0.1246 | 0.2839 | 0.0563 | 0.0026 | 0.0078 | 0.0073 | 0.1017 | 0.2627 | 0.0593 | 0.0115 | 0.0042 | 0.0067 |
Cell Periphery | 0.013 | 0.0045 | 0.0049 | 0.0051 | 0.0115 | 0.0053 | 0.0363 | 0.0087 | 0.0073 | 0.0076 | 0.013 | 0.0054 | 0.0187 | 0.003 | 0.0048 | 0.0047 | 0.0034 | 0.0045 |
Cytoplasm | 0.1658 | 0.2754 | 0.1279 | 0.1329 | 0.2074 | 0.1303 | 0.2165 | 0.156 | 0.1351 | 0.2668 | 0.222 | 0.1094 | 0.1888 | 0.149 | 0.1039 | 0.1326 | 0.1828 | 0.0834 |
Cytoplasmic Foci | 0.0797 | 0.0525 | 0.0367 | 0.0408 | 0.0894 | 0.0583 | 0.0704 | 0.0647 | 0.0473 | 0.0481 | 0.0826 | 0.044 | 0.07 | 0.0572 | 0.0404 | 0.0373 | 0.0778 | 0.0457 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0012 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0155 | 0.0188 | 0.0064 | 0.0126 | 0.0106 | 0.0114 | 0.026 | 0.0292 | 0.0156 | 0.014 | 0.0203 | 0.0138 | 0.0218 | 0.0142 | 0.0111 | 0.026 | 0.0191 | 0.0096 |
Endosome | 0.1517 | 0.1487 | 0.1125 | 0.2486 | 0.2046 | 0.1039 | 0.1356 | 0.1886 | 0.1837 | 0.2443 | 0.3457 | 0.1221 | 0.1933 | 0.2633 | 0.2228 | 0.3484 | 0.3297 | 0.1298 |
Golgi | 0.0591 | 0.0389 | 0.0434 | 0.0744 | 0.0925 | 0.0428 | 0.0504 | 0.0518 | 0.069 | 0.1681 | 0.1178 | 0.0445 | 0.0544 | 0.0466 | 0.057 | 0.0572 | 0.0978 | 0.052 |
Lipid Particles | 0.0329 | 0.0112 | 0.0055 | 0.0077 | 0.0111 | 0.0125 | 0.0345 | 0.0169 | 0.0096 | 0.0068 | 0.0225 | 0.0087 | 0.0202 | 0.006 | 0.0049 | 0.0286 | 0.0172 | 0.0059 |
Mitochondria | 0.0272 | 0.0071 | 0.0127 | 0.0396 | 0.0311 | 0.0163 | 0.0112 | 0.0066 | 0.0228 | 0.0056 | 0.0086 | 0.0064 | 0.0092 | 0.0106 | 0.0172 | 0.0121 | 0.011 | 0.0069 |
None | 0.008 | 0.0123 | 0.0066 | 0.0031 | 0.0121 | 0.003 | 0.0158 | 0.0162 | 0.0034 | 0.002 | 0.0022 | 0.012 | 0.0078 | 0.0047 | 0.0058 | 0.0058 | 0.0045 | 0.007 |
Nuclear Periphery | 0.0092 | 0.0029 | 0.0022 | 0.0029 | 0.003 | 0.0007 | 0.0036 | 0.003 | 0.0042 | 0.0019 | 0.0021 | 0.0006 | 0.0037 | 0.0026 | 0.0027 | 0.0052 | 0.0093 | 0.0013 |
Nucleolus | 0.0014 | 0.0008 | 0.0011 | 0.0006 | 0.006 | 0.0005 | 0.0013 | 0.0025 | 0.0008 | 0.0003 | 0.0002 | 0.0008 | 0.0014 | 0.0012 | 0.0011 | 0.0004 | 0.0005 | 0.0005 |
Nucleus | 0.0039 | 0.0029 | 0.0032 | 0.0025 | 0.0219 | 0.0016 | 0.0022 | 0.0121 | 0.0019 | 0.001 | 0.0012 | 0.0011 | 0.0025 | 0.0027 | 0.0026 | 0.0018 | 0.0022 | 0.0013 |
Peroxisomes | 0.0144 | 0.0021 | 0.0057 | 0.0018 | 0.0071 | 0.0047 | 0.0093 | 0.0018 | 0.0119 | 0.0011 | 0.0037 | 0.0027 | 0.0032 | 0.002 | 0.0022 | 0.0095 | 0.0077 | 0.0032 |
Punctate Nuclear | 0.0034 | 0.0016 | 0.002 | 0.0005 | 0.004 | 0.0024 | 0.0044 | 0.0047 | 0.001 | 0.0003 | 0.0004 | 0.0004 | 0.0024 | 0.0012 | 0.0008 | 0.0019 | 0.0023 | 0.0017 |
Vacuole | 0.0509 | 0.0511 | 0.0384 | 0.088 | 0.0592 | 0.0219 | 0.0559 | 0.0564 | 0.0617 | 0.0664 | 0.0875 | 0.0383 | 0.1006 | 0.0895 | 0.082 | 0.1272 | 0.1044 | 0.0403 |
Vacuole Periphery | 0.0062 | 0.0052 | 0.0072 | 0.0164 | 0.0055 | 0.0034 | 0.0052 | 0.0048 | 0.0121 | 0.0082 | 0.0085 | 0.005 | 0.0067 | 0.009 | 0.0161 | 0.0189 | 0.0156 | 0.0054 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.4728 | 33.0579 | 18.5635 | 28.3296 | 41.467 | 28.9683 | 22.8102 | 24.3886 | 19.8917 | 48.1946 |
Translational Efficiency | 1.4383 | 1.1306 | 1.3043 | 0.852 | 1.1742 | 2.1978 | 1.5949 | 0.8982 | 1.0371 | 1.0158 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1298 | 2238 | 326 | 957 | 1878 | 1329 | 277 | 1506 | 3176 | 3567 | 603 | 2463 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 787.64 | 947.37 | 1054.28 | 1156.76 | 840.63 | 871.19 | 1168.66 | 1243.69 | 818.97 | 918.99 | 1106.82 | 1209.91 |
Standard Deviation | 96.51 | 128.09 | 120.54 | 174.57 | 109.91 | 115.72 | 119.97 | 189.88 | 107.84 | 129.00 | 133.10 | 188.90 |
Intensity Change Log 2 | — | 0.266392 | 0.420650 | 0.554481 | — | 0.051516 | 0.475312 | 0.565084 | — | 0.159457 | 0.449129 | 0.559965 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015255 | 0.032130 | 0.003077 | 0.055029 | 0.033038 | 0.020279 | 0.032057 | 0.041405 | 0.025770 | 0.027714 | 0.016390 | 0.046699 |
Bud Neck | 0.157892 | 0.101106 | 0.014120 | 0.048921 | 0.165953 | 0.139996 | 0.058915 | 0.061843 | 0.162659 | 0.115596 | 0.034697 | 0.056822 |
Bud Site | 0.155629* | 0.226763* | 0.048573 | 0.364800* | 0.181107* | 0.217089* | 0.295771* | 0.408651* | 0.170695* | 0.223159* | 0.162128* | 0.391613* |
Cell Periphery | 0.011861 | 0.011064 | 0.000489 | 0.074716 | 0.031094 | 0.030885 | 0.018313 | 0.098868 | 0.023234 | 0.018449 | 0.008677 | 0.089484 |
Cytoplasm | 0.259527* | 0.173446* | 0.047779 | 0.057795 | 0.243391* | 0.168717* | 0.038355 | 0.069045 | 0.249985* | 0.171684* | 0.043450 | 0.064674 |
Cytoplasmic Foci | 0.118796 | 0.119269 | 0.088515 | 0.030718 | 0.062822 | 0.070850 | 0.052594 | 0.031029 | 0.085698 | 0.101229 | 0.072014 | 0.030909 |
Eisosomes | 0.007715 | 0.004420 | 0.000128 | 0.003350 | 0.013280 | 0.007596 | 0.000679 | 0.003384 | 0.011005 | 0.005603 | 0.000381 | 0.003371 |
Endoplasmic Reticulum | 0.024345 | 0.036520 | 0.044433 | 0.044075 | 0.029789 | 0.052911 | 0.070592 | 0.039950 | 0.027564 | 0.042627 | 0.056450 | 0.041553 |
Endosome | 0.062481 | 0.085604 | 0.148413 | 0.060812 | 0.060950 | 0.077560 | 0.098657 | 0.053488 | 0.061576 | 0.082607 | 0.125556 | 0.056334 |
Golgi | 0.037454 | 0.060684 | 0.008780 | 0.020314 | 0.040422 | 0.045560 | 0.021036 | 0.025561 | 0.039209 | 0.055049 | 0.014410 | 0.023522 |
Lipid Particles | 0.025355 | 0.028205 | 0.024702 | 0.016040 | 0.029963 | 0.025404 | 0.015034 | 0.024235 | 0.028080 | 0.027161 | 0.020261 | 0.021051 |
Mitochondria | 0.013890 | 0.021475 | 0.000904 | 0.003389 | 0.015974 | 0.014668 | 0.000871 | 0.006059 | 0.015122 | 0.018939 | 0.000889 | 0.005022 |
Mitotic Spindle | 0.000301 | 0.000245 | 0.005215 | 0.002116 | 0.000085 | 0.000793 | 0.000749 | 0.000887 | 0.000173 | 0.000449 | 0.003164 | 0.001364 |
None | 0.000580 | 0.000211 | 0.000141 | 0.000063 | 0.000120 | 0.000139 | 0.000012 | 0.000163 | 0.000308 | 0.000184 | 0.000081 | 0.000124 |
Nuclear Periphery | 0.000130 | 0.000063 | 0.005654 | 0.000098 | 0.000055 | 0.000107 | 0.000117 | 0.000082 | 0.000086 | 0.000079 | 0.003111 | 0.000088 |
Nuclear Periphery Foci | 0.000848 | 0.000387 | 0.018899 | 0.001240 | 0.000305 | 0.000545 | 0.002275 | 0.000691 | 0.000527 | 0.000446 | 0.011263 | 0.000904 |
Nucleolus | 0.000573 | 0.000193 | 0.003835 | 0.000129 | 0.000159 | 0.000141 | 0.000200 | 0.000264 | 0.000328 | 0.000173 | 0.002165 | 0.000212 |
Nucleus | 0.007190 | 0.002272 | 0.036965 | 0.001130 | 0.003053 | 0.002308 | 0.001074 | 0.001461 | 0.004744 | 0.002285 | 0.020477 | 0.001332 |
Peroxisomes | 0.018495 | 0.017117 | 0.005443 | 0.003759 | 0.024298 | 0.019934 | 0.006834 | 0.005491 | 0.021926 | 0.018166 | 0.006082 | 0.004818 |
Vacuole | 0.073568 | 0.064226 | 0.465419* | 0.188925 | 0.056467 | 0.090575 | 0.270457* | 0.110977 | 0.063456 | 0.074043 | 0.375860* | 0.141264 |
Vacuole Periphery | 0.008115 | 0.014602 | 0.028516 | 0.022581 | 0.007674 | 0.013940 | 0.015408 | 0.016464 | 0.007855 | 0.014355 | 0.022495 | 0.018841 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.97 | 6.84 | -7.93 | -4.40 | -10.59 | 4.92 | 0.16 | -2.42 | -6.67 | -1.45 | -1.01 | 3.02 | -7.30 | -6.63 | -8.07 |
Bud Neck | 7.35 | 20.10 | 14.98 | 10.39 | -8.32 | 3.10 | 11.83 | 16.56 | 11.40 | -0.39 | 8.58 | 22.64 | 22.21 | 14.59 | -5.13 |
Bud Site | -8.73 | 11.30 | -17.00 | -11.38 | -24.36* | -3.94 | -5.77 | -22.41 | -16.75 | -5.36 | -8.79 | 0.68 | -28.17 | -20.79 | -17.92 |
Cell Periphery | 0.61 | 11.26 | -11.41 | -11.63 | -13.75 | 0.08 | 3.90 | -12.68 | -12.35 | -13.92 | 3.25 | 8.38 | -16.93 | -18.36 | -20.32 |
Cytoplasm | 11.65 | 27.08 | 28.25 | 22.10 | -1.75 | 10.24 | 29.70 | 29.26 | 16.38 | -5.66 | 15.50 | 40.01 | 40.44 | 27.36 | -5.37 |
Cytoplasmic Foci | -0.07 | 4.33 | 19.00 | 23.20 | 9.41 | -2.13 | 1.54 | 10.63 | 10.98 | 3.42 | -5.01 | 2.84 | 20.78 | 26.15 | 9.24 |
Eisosomes | 5.16 | 13.22 | 6.06 | 2.10 | -7.16 | 5.57 | 15.70 | 10.98 | 5.49 | -6.04 | 8.93 | 20.04 | 12.48 | 5.22 | -9.29 |
Endoplasmic Reticulum | -4.35 | -3.08 | -4.73 | -1.76 | 0.00 | -6.20 | -4.71 | -3.17 | 3.05 | 3.44 | -6.85 | -5.42 | -5.49 | 0.41 | 2.64 |
Endosome | -6.27 | -10.08 | 0.39 | 6.03 | 10.04 | -4.37 | -4.86 | 2.13 | 6.05 | 5.77 | -8.04 | -10.84 | 1.94 | 9.36 | 11.57 |
Golgi | -7.76 | 11.96 | 6.21 | 13.66 | -4.90 | -1.59 | 5.67 | 5.56 | 6.10 | -1.31 | -7.20 | 12.11 | 8.04 | 14.21 | -4.40 |
Lipid Particles | -1.48 | 0.17 | 4.12 | 5.44 | 2.83 | 2.03 | 3.44 | 2.23 | 0.44 | -1.99 | 0.63 | 3.03 | 3.90 | 3.37 | -0.26 |
Mitochondria | -4.98 | 11.37 | 8.11 | 14.79 | -3.57 | 1.01 | 17.95 | 8.98 | 6.97 | -6.87 | -3.80 | 20.80 | 11.96 | 15.31 | -7.68 |
Mitotic Spindle | 0.71 | -2.25 | -2.23 | -2.32 | 1.37 | -2.04 | -2.96 | -3.89 | -0.24 | -0.47 | -2.00 | -2.51 | -3.51 | -2.53 | 1.49 |
None | 1.91 | 2.29 | 2.73 | 3.29 | 2.03 | -0.38 | 6.34 | -0.59 | -0.32 | -1.92 | 1.48 | 2.82 | 1.95 | 0.94 | -0.86 |
Nuclear Periphery | 2.69 | -4.80 | 1.42 | -2.49 | 4.83 | -6.42 | -3.77 | -4.17 | 2.48 | 1.99 | 0.58 | -4.79 | -0.23 | -1.00 | 4.79 |
Nuclear Periphery Foci | 1.68 | -6.82 | -1.05 | -8.58 | 6.74 | -5.39 | -7.66 | -7.14 | -2.26 | 6.04 | 0.81 | -7.30 | -2.66 | -8.39 | 7.08 |
Nucleolus | 1.60 | -3.22 | 1.86 | 1.54 | 3.81 | 0.99 | -0.34 | -0.79 | -0.92 | -0.35 | 1.57 | -3.36 | 0.99 | -0.44 | 3.62 |
Nucleus | 9.30 | -5.51 | 11.40 | 8.04 | 6.66 | 3.34 | 6.61 | 4.63 | 2.54 | -1.33 | 9.71 | -5.24 | 11.42 | 4.80 | 6.36 |
Peroxisomes | 1.07 | 10.20 | 13.33 | 14.90 | 2.22 | 2.34 | 12.33 | 15.32 | 9.38 | 1.43 | 3.45 | 16.26 | 19.93 | 16.46 | 2.14 |
Vacuole | 2.11 | -25.98 | -13.22 | -14.78 | 16.36 | -6.61 | -12.67 | -10.22 | -3.25 | 9.14 | -3.35 | -26.27 | -16.31 | -13.74 | 18.66 |
Vacuole Periphery | -6.60 | -5.50 | -7.03 | -3.91 | 1.28 | -5.79 | -3.89 | -7.27 | -1.68 | -0.51 | -9.59 | -6.65 | -10.06 | -3.85 | 1.35 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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