Standard name
Description Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.22 0.23 0.19 0.11 0.1 0 0 – 0 0 0 0.23 0.25 0.13 0.05 0.15 0.13 0.15 0.14 0.26 0.17 0.18 0.15
Bud 0 0.06 0.15 0.09 0.11 0.11 0.1 0.09 – 0.08 0.1 0.09 0.11 0.09 0 0 0 0 0.06 0.11 0 0.18 0.21 0.16
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0.12 0.06 0.15 0.12 0.1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 0.35 0.52 0.42 0.59 0.65 0.68 0.7 0.74 – 0.74 0.78 0.75 0.46 0.47 0.59 0.44 0.42 0.38 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.24 0.27 0.22 0.13 0.06 0.08 0 0 – 0 0 0 0.27 0.27 0.26 0 0 0 0.27 0.24 0.23 0.07 0.08 0.12
Endosome 0.06 0 0.05 0 0.1 0 0.05 0 – 0 0 0 0 0.05 0.06 0.05 0.06 0 0.06 0 0.1 0.06 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0.08 0 0 0.09 0.15 0.14 – 0.13 0.23 0.22 0 0 0 0.07 0.07 0 0 0 0.06 0.06 0.06 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.07 0 0.07 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0 0 0 0.07 0 0.05 0.05 – 0.08 0 0.05 0 0 0.07 0.44 0.41 0.4 0.28 0.24 0.17 0.2 0.23 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 54 30 74 64 52 68 30 11 – 19 17 14 30 38 22 4 12 7 47 25 104 21 47 35
Bud 10 8 47 32 53 75 87 74 – 62 61 65 14 14 2 0 3 0 17 20 14 22 53 35
Bud Neck 0 0 0 1 2 1 3 0 – 3 1 1 0 0 0 0 0 0 28 21 22 18 31 22
Bud Site 7 6 9 11 7 14 10 5 – 1 4 3 1 1 0 0 0 0 – – – – – –
Cell Periphery 4 1 3 3 2 1 7 8 – 11 7 5 6 12 8 0 0 1 1 0 4 0 0 0
Cytoplasm 83 72 133 202 320 452 586 617 – 595 452 551 60 72 101 32 34 21 6 10 7 3 5 5
Endoplasmic Reticulum 57 37 70 45 29 51 28 19 – 20 5 12 36 41 44 3 2 2 84 43 93 8 20 28
Endosome 14 2 17 6 49 28 39 24 – 35 5 8 1 7 11 4 5 1 18 6 40 7 14 14
Golgi 0 0 0 1 0 0 0 0 – 0 0 0 0 1 1 2 0 3 10 1 13 1 1 4
Mitochondria 1 2 26 11 13 62 124 121 – 107 133 163 1 0 0 5 6 2 4 3 24 8 14 8
Nucleus 1 0 0 2 3 3 4 10 – 5 2 3 0 0 0 0 0 0 0 0 0 0 2 1
Nuclear Periphery 0 0 1 2 1 5 2 4 – 4 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 1 1 0 1 0 3 10 – 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 18 10 11 24 23 9 14 4 – 3 3 2 1 2 0 0 1 0 1 0 3 3 2 1
SpindlePole 3 2 7 8 13 13 19 13 – 10 2 9 1 2 1 0 1 0 2 2 0 0 0 1
Vac/Vac Membrane 44 5 11 8 32 30 40 42 – 61 23 37 2 4 12 32 33 22 88 42 70 25 60 64
Unique Cell Count 240 139 318 342 492 667 835 836 800 583 733 131 154 172 73 81 55 315 181 407 125 258 232
Labelled Cell Count 296 176 410 420 600 812 996 962 939 715 874 153 194 202 82 97 59 315 181 407 125 258 232


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 8.6 6.0 6.0 5.8 5.5 4.6 4.9 – 4.5 4.3 4.3 7.6 8.1 8.5 10.9 10.2 10.7 8.7 9.0 8.8
Std Deviation (1e-4) 1.1 1.5 1.0 1.4 1.2 1.5 1.1 1.2 – 1.3 0.9 0.9 1.1 1.4 1.7 2.4 2.4 2.4 1.4 1.7 2.1
Intensity Change (Log2) – – – 0.01 -0.03 -0.11 -0.38 -0.3 – -0.39 -0.47 -0.48 0.34 0.44 0.51 0.86 0.78 0.84 0.55 0.59 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -4.9 -5.5 0 0 – 0 0 0 -0.1 0.3 -2.8 -3.4 -1.7 -1.8
Bud -2.1 -1.7 -1.6 0 0 – 0 0 0 -1.2 -1.7 -4.8 -3.5 -2.7 -3.0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0.3 -1.4 -0.7 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 4.4 6.5 7.8 8.9 10.2 – 10.3 10.7 10.4 0.8 1.0 3.6 0.3 0 -0.5
Endoplasmic Reticulum -3.0 -6.8 -6.4 0 0 – 0 0 0 1.2 1.1 0.9 -3.5 -4.1 -3.2
Endosome -2.5 2.3 -0.8 0 0 – 0 0 0 -2.3 -0.4 0.5 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -2.8 -3.6 0.6 0 0 – 0 0 0 -3.0 -3.7 -3.9 -0.4 -0.2 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 2.0 0.8 -2.2 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0.4 -0.3 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -0.9 1.9 0.8 1.0 1.1 – 2.6 0.4 1.1 0 0 1.8 9.9 9.6 0