Standard name | |
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Description | Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 54 | 30 | 74 | 64 | 52 | 68 | 30 | 11 | – | 19 | 17 | 14 | 30 | 38 | 22 | 4 | 12 | 7 | 47 | 25 | 104 | 21 | 47 | 35 |
Bud | 10 | 8 | 47 | 32 | 53 | 75 | 87 | 74 | – | 62 | 61 | 65 | 14 | 14 | 2 | 0 | 3 | 0 | 17 | 20 | 14 | 22 | 53 | 35 |
Bud Neck | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 0 | – | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 28 | 21 | 22 | 18 | 31 | 22 |
Bud Site | 7 | 6 | 9 | 11 | 7 | 14 | 10 | 5 | – | 1 | 4 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 3 | 3 | 2 | 1 | 7 | 8 | – | 11 | 7 | 5 | 6 | 12 | 8 | 0 | 0 | 1 | 1 | 0 | 4 | 0 | 0 | 0 |
Cytoplasm | 83 | 72 | 133 | 202 | 320 | 452 | 586 | 617 | – | 595 | 452 | 551 | 60 | 72 | 101 | 32 | 34 | 21 | 6 | 10 | 7 | 3 | 5 | 5 |
Endoplasmic Reticulum | 57 | 37 | 70 | 45 | 29 | 51 | 28 | 19 | – | 20 | 5 | 12 | 36 | 41 | 44 | 3 | 2 | 2 | 84 | 43 | 93 | 8 | 20 | 28 |
Endosome | 14 | 2 | 17 | 6 | 49 | 28 | 39 | 24 | – | 35 | 5 | 8 | 1 | 7 | 11 | 4 | 5 | 1 | 18 | 6 | 40 | 7 | 14 | 14 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 3 | 10 | 1 | 13 | 1 | 1 | 4 |
Mitochondria | 1 | 2 | 26 | 11 | 13 | 62 | 124 | 121 | – | 107 | 133 | 163 | 1 | 0 | 0 | 5 | 6 | 2 | 4 | 3 | 24 | 8 | 14 | 8 |
Nucleus | 1 | 0 | 0 | 2 | 3 | 3 | 4 | 10 | – | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 1 | 5 | 2 | 4 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 10 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 18 | 10 | 11 | 24 | 23 | 9 | 14 | 4 | – | 3 | 3 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 3 | 2 | 1 |
SpindlePole | 3 | 2 | 7 | 8 | 13 | 13 | 19 | 13 | – | 10 | 2 | 9 | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 44 | 5 | 11 | 8 | 32 | 30 | 40 | 42 | – | 61 | 23 | 37 | 2 | 4 | 12 | 32 | 33 | 22 | 88 | 42 | 70 | 25 | 60 | 64 |
Unique Cell Count | 240 | 139 | 318 | 342 | 492 | 667 | 835 | 836 | 800 | 583 | 733 | 131 | 154 | 172 | 73 | 81 | 55 | 315 | 181 | 407 | 125 | 258 | 232 | |
Labelled Cell Count | 296 | 176 | 410 | 420 | 600 | 812 | 996 | 962 | 939 | 715 | 874 | 153 | 194 | 202 | 82 | 97 | 59 | 315 | 181 | 407 | 125 | 258 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.2 | 8.6 | 6.0 | 6.0 | 5.8 | 5.5 | 4.6 | 4.9 | – | 4.5 | 4.3 | 4.3 | 7.6 | 8.1 | 8.5 | 10.9 | 10.2 | 10.7 | 8.7 | 9.0 | 8.8 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.0 | 1.4 | 1.2 | 1.5 | 1.1 | 1.2 | – | 1.3 | 0.9 | 0.9 | 1.1 | 1.4 | 1.7 | 2.4 | 2.4 | 2.4 | 1.4 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.03 | -0.11 | -0.38 | -0.3 | – | -0.39 | -0.47 | -0.48 | 0.34 | 0.44 | 0.51 | 0.86 | 0.78 | 0.84 | 0.55 | 0.59 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -4.9 | -5.5 | 0 | 0 | – | 0 | 0 | 0 | -0.1 | 0.3 | -2.8 | -3.4 | -1.7 | -1.8 |
Bud | -2.1 | -1.7 | -1.6 | 0 | 0 | – | 0 | 0 | 0 | -1.2 | -1.7 | -4.8 | -3.5 | -2.7 | -3.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.3 | -1.4 | -0.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.4 | 6.5 | 7.8 | 8.9 | 10.2 | – | 10.3 | 10.7 | 10.4 | 0.8 | 1.0 | 3.6 | 0.3 | 0 | -0.5 |
Endoplasmic Reticulum | -3.0 | -6.8 | -6.4 | 0 | 0 | – | 0 | 0 | 0 | 1.2 | 1.1 | 0.9 | -3.5 | -4.1 | -3.2 |
Endosome | -2.5 | 2.3 | -0.8 | 0 | 0 | – | 0 | 0 | 0 | -2.3 | -0.4 | 0.5 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.8 | -3.6 | 0.6 | 0 | 0 | – | 0 | 0 | 0 | -3.0 | -3.7 | -3.9 | -0.4 | -0.2 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2.0 | 0.8 | -2.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.1 | 0.4 | -0.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.9 | 1.9 | 0.8 | 1.0 | 1.1 | – | 2.6 | 0.4 | 1.1 | 0 | 0 | 1.8 | 9.9 | 9.6 | 0 |