Standard name
Human Ortholog
Description Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0.09 0 0.08 0.06 0 0.05 – 0 0 0.05 0.07 0.11 0.14 0.19 0.22 0.24 0.1 0 0 0 0.06 0.09
Bud – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0.05 0 0 0 0 – 0 0 0.05 0.05 0.1 0.2 0.21 0.25 0.16 0.08 0 0 0 0 0
Cytoplasm – – 0.09 0.12 0.13 0.14 0.09 0.09 – 0.08 0.08 0.06 0.1 0.08 0.14 0.2 0.16 0.18 0 0 0 0 0 0
Endoplasmic Reticulum – – 0.75 0.59 0.56 0.68 0.55 0.47 – 0.38 0.33 0.36 0.83 0.75 0.65 0 0 0.06 0.62 0.81 0.82 0.78 0.75 0.68
Endosome – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0.26 0.28 0.23 0 0 0 0 0 0
Mitochondria – – 0.21 0.17 0.14 0.18 0.32 0.28 – 0.32 0.46 0.5 0 0 0 0.1 0.07 0.11 0.07 0.06 0 0 0 0
Nucleus – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – – 0.09 0.1 0.05 0.06 0.11 0.11 – 0.1 0.05 0.09 0 0 0 0 0.05 0.07 0 0.05 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 0 0.18 0.22 0.08 0.21 0.25 – 0.23 0.26 0.22 0.07 0.14 0.06 0.2 0.19 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 5 6 19 7 9 11 – 14 7 17 12 25 34 38 36 29 1 0 3 2 10 18
Bud – – 1 0 3 4 2 5 – 11 7 4 2 3 4 5 4 3 0 0 0 0 2 1
Bud Neck – – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site – – 0 0 0 0 0 0 – 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 7 9 0 8 6 – 8 3 18 9 21 47 43 41 19 0 0 0 1 1 7
Cytoplasm – – 5 16 32 16 27 21 – 25 22 20 19 17 32 40 27 22 0 0 0 0 6 5
Endoplasmic Reticulum – – 43 79 135 76 165 113 – 120 94 125 151 164 152 9 2 7 6 26 65 46 138 147
Endosome – – 0 2 8 0 2 2 – 11 0 5 2 1 2 3 0 0 0 0 0 0 1 4
Golgi – – 0 1 0 0 0 1 – 2 1 1 0 2 4 52 47 27 0 0 0 1 4 8
Mitochondria – – 12 23 34 20 95 67 – 99 132 176 2 1 4 19 11 13 0 1 1 2 5 6
Nucleus – – 0 0 2 1 3 2 – 1 2 1 0 0 1 1 1 1 0 0 0 0 0 1
Nuclear Periphery – – 5 14 11 7 33 26 – 30 15 32 2 2 0 5 9 8 0 1 3 2 3 0
Nucleolus – – 0 1 0 0 2 2 – 2 4 4 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0 1 0 0 – 1 0 0 0 1 2 0 1 1 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 – 2 0 0 0 0 0 2 3 0 0 0 0 0 0 0
Vac/Vac Membrane – – 1 24 54 9 62 60 – 72 74 78 13 30 14 40 31 17 0 0 2 0 3 8
Unique Cell Count 57 134 241 112 298 243 314 287 351 183 220 235 200 167 119 11 33 80 59 184 218
Labelled Cell Count 72 173 307 141 408 316 400 362 482 213 267 296 257 213 147 11 33 80 59 184 218


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 7.3 8.5 8.6 7.9 7.5 7.6 – 7.4 7.4 7.3 10.0 10.1 9.8 13.4 14.0 13.8 – 12.1 12.7
Std Deviation (1e-4) – – 0.8 1.3 1.6 1.5 1.5 1.3 – 1.5 1.3 1.4 1.3 1.4 1.6 2.8 2.9 3.0 – 2.4 2.5
Intensity Change (Log2) – – – 0.22 0.24 0.12 0.05 0.07 – 0.03 0.03 0.0 0.46 0.48 0.43 0.88 0.95 0.93 – 0.73 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0.6 1.1 1.8 2.2 2.5
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 3.7 3.8 4.1 3.2
Cytoplasm 0.6 0.9 1.0 0.1 0 – 0 0 0 0.4 0 1.0 2.0 1.4 1.7
Endoplasmic Reticulum -2.2 -2.7 -1.0 -2.8 -3.9 – -5.2 -6.0 -5.7 1.2 -0.1 -1.5 -11.8 -12.1 -9.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 4.3 4.5 3.9
Mitochondria -0.6 -1.3 -0.5 1.6 1.0 – 1.6 3.5 4.1 0 0 0 -2.4 -3.1 -1.8
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.4 0 0 0.5 0.4 – 0.2 0 0.1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 3.0 3.6 0 3.4 3.9 – 3.7 4.0 3.6 0 2.5 0 3.3 3.1 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.642 11.1904 9.9783 9.9299 8.8132 9.8811 10.6504 12.6851 11.7336 11.0117 10.4666 11.9566 9.2289 10.4267 9.7157 9.1784 8.3527 10.1024
Actin 0.0711 0.0592 0.0592 0.0542 0.0228 0.027 0.0513 0.0218 0.0231 0.0017 0.0326 0.0055 0.065 0.0416 0.03 0.0212 0.0028 0.0323
Bud 0.0016 0.0003 0.0005 0.0002 0.0003 0.0002 0.0004 0.0002 0.0003 0.0001 0.0004 0.0001 0.0004 0.0006 0.0003 0.0001 0.0002 0.0001
Bud Neck 0.0052 0.003 0.004 0.0149 0.0008 0.001 0.0013 0.0005 0.001 0.0004 0.0005 0.0015 0.0016 0.0029 0.0015 0.003 0.0003 0.0021
Bud Periphery 0.0044 0.0008 0.0008 0.0004 0.0005 0.0007 0.0016 0.0003 0.0007 0.0005 0.0005 0.0003 0.0014 0.0021 0.0006 0.0003 0.0003 0.0005
Bud Site 0.0103 0.0058 0.0056 0.0031 0.0006 0.0002 0.0025 0.0007 0.0009 0.0009 0.0015 0 0.0014 0.0023 0.004 0.0002 0.0013 0.0002
Cell Periphery 0.0215 0.0186 0.0188 0.0036 0.0016 0.0073 0.0108 0.0162 0.01 0.0024 0.0011 0.0063 0.0116 0.0164 0.008 0.0011 0.0005 0.008
Cytoplasm 0.0248 0.0174 0.0444 0.0044 0.0036 0.0035 0.0296 0.0276 0.0026 0.001 0.0019 0.0038 0.0061 0.0075 0.0135 0.0016 0.001 0.0044
Cytoplasmic Foci 0.0377 0.0074 0.0147 0.0121 0.0078 0.0063 0.0254 0.0026 0.0064 0.0007 0.0102 0.003 0.0182 0.0076 0.0066 0.0027 0.0184 0.0027
Eisosomes 0.005 0.0053 0.006 0.001 0.0005 0.0024 0.0088 0.0054 0.0112 0.0009 0.0002 0.0014 0.012 0.0061 0.0139 0.0002 0.0001 0.0042
Endoplasmic Reticulum 0.5067 0.7102 0.6658 0.817 0.6712 0.7634 0.6685 0.8701 0.8263 0.8891 0.792 0.8734 0.6255 0.7289 0.8092 0.8678 0.7851 0.8463
Endosome 0.0422 0.0145 0.0206 0.0079 0.0256 0.0237 0.0287 0.0051 0.0116 0.0177 0.0213 0.0105 0.0356 0.0207 0.0128 0.0156 0.018 0.0103
Golgi 0.0542 0.0576 0.0884 0.0161 0.069 0.0388 0.0641 0.0214 0.0325 0.0133 0.0298 0.026 0.0904 0.0554 0.0379 0.0253 0.0089 0.0503
Lipid Particles 0.0921 0.0729 0.0347 0.0067 0.0437 0.0502 0.0279 0.0168 0.0389 0.0068 0.0197 0.0359 0.0784 0.071 0.0337 0.0286 0.0214 0.0103
Mitochondria 0.0056 0.007 0.0034 0.0006 0.0336 0.0207 0.0323 0.0004 0.0133 0.0019 0.0212 0.0015 0.0105 0.0049 0.0044 0.0021 0.0069 0.0013
None 0.0032 0.0005 0.0021 0.0068 0.0017 0.001 0.0082 0.0003 0.0003 0.0004 0.0003 0.0002 0.007 0.0076 0.0054 0.0002 0.0007 0.0004
Nuclear Periphery 0.0675 0.0136 0.0116 0.0345 0.0724 0.0418 0.0137 0.0077 0.0084 0.021 0.0488 0.0257 0.0207 0.0129 0.0085 0.0229 0.099 0.0206
Nucleolus 0.0041 0.0001 0.0039 0.0016 0.0023 0.0003 0.0004 0 0 0.0011 0.0001 0 0.0003 0.0002 0.0004 0.0001 0.0003 0
Nucleus 0.0042 0.0004 0.0029 0.0013 0.008 0.0027 0.0013 0.0002 0.0002 0.0014 0.0008 0.0004 0.0012 0.0009 0.0006 0.0006 0.003 0.0004
Peroxisomes 0.0101 0.0009 0.004 0.0027 0.0086 0.0013 0.0109 0.0001 0.0085 0.0002 0.0112 0.0001 0.0029 0.0005 0.0034 0.0003 0.0198 0.0009
Punctate Nuclear 0.015 0.0009 0.0048 0.0068 0.0015 0.0009 0.0056 0.0001 0.0003 0.0001 0.0003 0.0001 0.0037 0.0026 0.0021 0.0002 0.0061 0.0009
Vacuole 0.0091 0.0026 0.0029 0.0021 0.0095 0.0036 0.0044 0.002 0.0022 0.0331 0.0028 0.0022 0.0038 0.0055 0.0018 0.0036 0.0024 0.0022
Vacuole Periphery 0.0044 0.0009 0.0011 0.002 0.0143 0.0029 0.0024 0.0006 0.0013 0.0052 0.0028 0.0019 0.0024 0.0019 0.0012 0.0023 0.0037 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 200.4143 240.2195 234.0196 188.2817 201.4535 190.5507 244.921 209.7224 189.7857 181.4945
Translational Efficiency 2.0498 2.0579 1.8279 1.991 2.132 2.3202 1.7816 1.8905 2.0287 1.9477

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Scs2

Scs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available