Standard name
Description Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0 0.08 0.06 0 0.05 0 0 0.05 0.07 0.11 0.14 0.19 0.22 0.24 0.1 0 0 0 0.06 0.09
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0.05 0.05 0.1 0.2 0.21 0.25 0.16 0.08 0 0 0 0 0
Cytoplasm 0.09 0.12 0.13 0.14 0.09 0.09 0.08 0.08 0.06 0.1 0.08 0.14 0.2 0.16 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0.75 0.59 0.56 0.68 0.55 0.47 0.38 0.33 0.36 0.83 0.75 0.65 0 0 0.06 0.62 0.81 0.82 0.78 0.75 0.68
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.26 0.28 0.23 0 0 0 0 0 0
Mitochondria 0.21 0.17 0.14 0.18 0.32 0.28 0.32 0.46 0.5 0 0 0 0.1 0.07 0.11 0.07 0.06 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.09 0.1 0.05 0.06 0.11 0.11 0.1 0.05 0.09 0 0 0 0 0.05 0.07 0 0.05 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.18 0.22 0.08 0.21 0.25 0.23 0.26 0.22 0.07 0.14 0.06 0.2 0.19 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 19 7 9 11 14 7 17 12 25 34 38 36 29 1 0 3 2 10 18
Bud 1 0 3 4 2 5 11 7 4 2 3 4 5 4 3 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0
Cell Periphery 0 7 9 0 8 6 8 3 18 9 21 47 43 41 19 0 0 0 1 1 7
Cytoplasm 5 16 32 16 27 21 25 22 20 19 17 32 40 27 22 0 0 0 0 6 5
Endoplasmic Reticulum 43 79 135 76 165 113 120 94 125 151 164 152 9 2 7 6 26 65 46 138 147
Endosome 0 2 8 0 2 2 11 0 5 2 1 2 3 0 0 0 0 0 0 1 4
Golgi 0 1 0 0 0 1 2 1 1 0 2 4 52 47 27 0 0 0 1 4 8
Mitochondria 12 23 34 20 95 67 99 132 176 2 1 4 19 11 13 0 1 1 2 5 6
Nucleus 0 0 2 1 3 2 1 2 1 0 0 1 1 1 1 0 0 0 0 0 1
Nuclear Periphery 5 14 11 7 33 26 30 15 32 2 2 0 5 9 8 0 1 3 2 3 0
Nucleolus 0 1 0 0 2 2 2 4 4 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 1 0 0 0 1 2 0 1 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 0 0 0 0 0 2 3 0 0 0 0 0 0 0
Vac/Vac Membrane 1 24 54 9 62 60 72 74 78 13 30 14 40 31 17 0 0 2 0 3 8
Unique Cell Count 57 134 241 112 298 243 314 287 351 183 220 235 200 167 119 11 33 80 59 184 218
Labelled Cell Count 72 173 307 141 408 316 400 362 482 213 267 296 257 213 147 11 33 80 59 184 218


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 8.5 8.6 7.9 7.5 7.6 7.4 7.4 7.3 10.0 10.1 9.8 13.4 14.0 13.8 12.1 12.7
Std Deviation (1e-4) 0.8 1.3 1.6 1.5 1.5 1.3 1.5 1.3 1.4 1.3 1.4 1.6 2.8 2.9 3.0 2.4 2.5
Intensity Change (Log2) 0.22 0.24 0.12 0.05 0.07 0.03 0.03 0.0 0.46 0.48 0.43 0.88 0.95 0.93 0.73 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0.6 1.1 1.8 2.2 2.5
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.7 3.8 4.1 3.2
Cytoplasm 0.6 0.9 1.0 0.1 0 0 0 0 0.4 0 1.0 2.0 1.4 1.7
Endoplasmic Reticulum -2.2 -2.7 -1.0 -2.8 -3.9 -5.2 -6.0 -5.7 1.2 -0.1 -1.5 -11.8 -12.1 -9.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 4.3 4.5 3.9
Mitochondria -0.6 -1.3 -0.5 1.6 1.0 1.6 3.5 4.1 0 0 0 -2.4 -3.1 -1.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.4 0 0 0.5 0.4 0.2 0 0.1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 3.6 0 3.4 3.9 3.7 4.0 3.6 0 2.5 0 3.3 3.1 2.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available