Standard name
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.16 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.1 0.09 0.11 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.05 0.08 0.12 0 0.05 0 0.06 0 0 0 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0.06 0.05 0 0.05 0 0 0.07 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0
Golgi 0.43 0.73 0.42 0.17 0.22 0.06 0 0 0 0 0 0 0.76 0.67 0.68 0.76 0.89 0.88 0.85 0.78 0.79 0.89 0.89 0.81
Mitochondria 0.83 0.36 0.81 0.85 0.72 0.91 0.91 0.91 0.9 0.9 0.93 0.95 0.56 0.55 0.43 0.1 0.11 0.08 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.12 0.06 0.08 0.12 0 0.05 0.08 0 0.05 0 0 0.11 0.13 0.13 0.19 0.16 0.16 0 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 54 7 0 1 5 0 4 6 2 2 3 15 14 4 31 23 20 2 6 5 3 8 14
Bud 3 0 1 1 0 0 0 4 3 3 3 6 1 4 3 0 1 1 0 1 2 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 0 0 2 0 0 0 1 0 0
Cell Periphery 0 1 0 0 0 0 0 2 3 2 0 5 0 1 2 2 2 2 0 0 0 0 0 0
Cytoplasm 2 25 8 8 14 8 9 6 12 6 7 4 6 9 16 15 16 10 1 4 1 0 1 0
Endoplasmic Reticulum 4 41 9 5 1 10 8 10 13 3 6 10 22 13 15 6 7 9 1 3 1 1 2 4
Endosome 0 0 1 0 1 0 1 0 0 0 0 0 3 3 2 10 4 0 7 7 10 4 4 8
Golgi 60 242 65 17 25 11 6 12 7 8 5 5 266 194 237 237 223 168 134 275 137 190 276 218
Mitochondria 115 119 126 84 81 172 175 253 166 176 172 220 195 158 150 30 27 16 4 11 8 5 7 6
Nucleus 0 0 0 0 1 1 0 1 0 3 0 0 1 0 2 2 3 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 1 1 3 3 3 2 4 3 0 0 0 2 2 0 0 0 0 1 0 0
Nucleolus 3 3 3 4 2 4 6 12 6 6 5 5 4 0 3 7 4 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 24 39 9 8 13 8 9 21 4 10 5 4 38 38 47 60 39 31 5 38 2 3 5 6
Unique Cell Count 138 333 155 99 112 189 193 277 185 196 185 231 348 289 349 313 251 190 158 354 174 214 311 268
Labelled Cell Count 219 524 229 127 140 221 217 328 226 222 209 267 551 434 481 403 351 258 158 354 174 214 311 268


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.7 20.1 13.0 11.2 11.6 11.2 9.3 9.4 9.6 9.4 9.2 9.0 16.8 16.1 16.4 17.2 22.3 23.2 14.6 14.6 14.7
Std Deviation (1e-4) 2.4 3.5 3.0 2.9 2.7 2.7 2.1 2.3 2.2 2.1 2.1 2.2 3.1 3.6 3.6 5.0 6.2 5.8 3.0 2.3 2.6
Intensity Change (Log2) -0.22 -0.17 -0.22 -0.48 -0.47 -0.44 -0.47 -0.5 -0.54 0.37 0.31 0.33 0.4 0.78 0.83 0.17 0.16 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 -0.9 0 0 -0.6 0 0 0 -0.1 0.2 0 2.0 1.7 2.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 2.2 -0.4 -0.2 -1.7 0.5 -1.0 -0.6 0 0 -1.1 -0.3 -0.2 0.5 0
Endoplasmic Reticulum -0.3 0 -0.2 -0.7 -1.1 0.5 -2.2 -1.1 -0.7 0.2 -0.6 -0.7 0 -1.5 -0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -4.1 -3.3 -8.0 -8.9 -9.8 -8.6 -8.7 -8.9 -9.9 7.5 5.1 5.5 7.2 10.1 9.2
Mitochondria 0.7 -1.7 2.6 2.5 3.1 2.2 2.3 3.3 4.4 -5.4 -5.6 -8.0 -15.5 -14.2 -13.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 1.7 -0.7 -0.5 0.7 -1.7 -0.3 -1.4 -2.2 1.8 2.4 2.5 3.8 3.0 3.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glo3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available