Standard name
Human Ortholog
Description Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.98 – 0.94 0.96 0.98 0.96 0.94 0.89 0.87 0.84 0.83 0.77 0.98 1.0 0.99 0.95 0.98 0.96 0.95 0.96 0.85 0.95 0.93 0.91
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.11 0.12 0.08 0.15 0.21 0.26 0.35 0.35 0.38 0.47 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud 0 – 1 3 3 3 17 13 2 13 3 12 1 1 10 0 0 0 0 0 0 0 2 4
Bud Neck 0 – 0 0 0 1 1 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 0 – 0 0 0 0 1 1 3 0 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 – 2 1 4 3 5 1 6 6 3 5 1 3 2 5 5 7 0 0 0 0 0 0
Cytoplasm 285 – 119 196 325 333 530 586 266 327 192 280 181 303 365 131 91 130 277 46 129 84 236 255
Endoplasmic Reticulum 3 – 0 0 0 0 0 1 1 0 0 2 2 1 0 9 6 5 2 0 0 0 0 3
Endosome 0 – 0 0 1 1 2 2 0 1 0 0 0 0 0 1 0 0 1 0 0 0 1 2
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0
Mitochondria 2 – 14 24 25 53 116 171 107 135 88 171 0 0 5 6 1 2 2 0 9 0 1 3
Nucleus 0 – 0 1 0 1 2 2 2 0 1 0 1 3 2 1 0 0 1 0 0 0 0 0
Nuclear Periphery 1 – 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 1 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 2 0 2 1 3 2 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 – 0 0 0 0 2 5 0 1 0 1 0 0 0 5 0 3 1 1 9 2 5 5
Unique Cell Count 290 126 204 331 346 565 658 305 389 230 363 185 304 368 138 93 136 293 48 152 88 255 280
Labelled Cell Count 294 137 226 359 395 678 787 390 486 292 475 187 311 384 159 104 148 293 48 152 88 255 280


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 – 3.7 3.6 3.7 3.5 3.2 3.3 3.0 3.1 3.2 2.9 5.5 5.4 4.7 7.0 6.7 7.2 5.2 5.2 5.9
Std Deviation (1e-4) 0.6 – 0.4 0.6 0.9 0.9 0.7 1.0 0.8 0.9 0.9 0.8 1.0 0.9 1.4 7.9 1.4 1.6 1.0 1.1 1.7
Intensity Change (Log2) – – – -0.01 0.02 -0.08 -0.19 -0.14 -0.28 -0.26 -0.22 -0.36 0.59 0.55 0.36 0.93 0.87 0.97 0.49 0.49 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 2.2 0.9 -0.3 -1.8 -2.2 -3.0 -3.0 -4.3 1.6 3.7 3.3 0.2 1.3 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.6 0 0 -2.1 -2.9 -3.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5883 1.683 1.0951 0.4744 0.5175 1.205 2.8285 2.4825 2.4007 1.7657 2.4303 2.6699 0.0133 0.3988 0.4719 0.3286 -0.0867 0.4478
Actin 0.0005 0.0004 0.0009 0.0007 0.0003 0.0004 0.0664 0.0023 0.0021 0.0001 0.0144 0.016 0.0257 0.0019 0.0188 0.0001 0.001 0.0027
Bud 0 0.0002 0.0002 0.0002 0.0001 0 0.0013 0.0003 0.002 0.0002 0.001 0.0006 0.0001 0.0099 0.0082 0.0002 0.0049 0.0022
Bud Neck 0.0001 0.0001 0.0004 0.0004 0.0003 0.0025 0.0054 0.0002 0.0004 0.0001 0.0009 0.0012 0.0003 0.0001 0.0008 0.0001 0.0015 0.0003
Bud Periphery 0.0001 0.0006 0.0003 0.0002 0.0001 0.0001 0.002 0.0003 0.0017 0.0001 0.0013 0.0008 0.0002 0.0018 0.0114 0.0001 0.0024 0.0014
Bud Site 0.0002 0.001 0.0013 0.0008 0.0002 0.0001 0.0082 0.0114 0.0073 0.0001 0.004 0.001 0.0004 0.0059 0.0036 0.0001 0.0097 0
Cell Periphery 0.0006 0.0013 0.0009 0.0005 0.0002 0.0002 0.0006 0.0003 0.0003 0.0001 0.0003 0.0001 0.0005 0.0003 0.0007 0.0001 0.0003 0.0002
Cytoplasm 0.6069 0.7212 0.7238 0.757 0.648 0.8487 0.4489 0.791 0.7403 0.8793 0.6437 0.812 0.5085 0.5952 0.4642 0.6661 0.6931 0.6435
Cytoplasmic Foci 0.0038 0.006 0.0092 0.0094 0.0059 0.0059 0.0332 0.0042 0.0181 0.0075 0.0298 0.0108 0.0051 0.0115 0.0217 0.0059 0.0259 0.0073
Eisosomes 0.0004 0.0002 0.0001 0.0002 0 0.0001 0.0015 0.0001 0 0 0.0002 0.0001 0.0002 0 0.0005 0 0 0.0001
Endoplasmic Reticulum 0.0063 0.0043 0.0056 0.0029 0.0035 0.002 0.0146 0.0019 0.0023 0.003 0.0023 0.0033 0.0063 0.0015 0.0032 0.0009 0.0022 0.002
Endosome 0.0022 0.0032 0.0073 0.0115 0.0054 0.0052 0.0431 0.0031 0.0049 0.0101 0.0535 0.0066 0.0053 0.0028 0.0077 0.0027 0.0133 0.0015
Golgi 0.0002 0.0006 0.0004 0.0004 0.0002 0.0002 0.013 0.0037 0.0035 0.0003 0.0227 0.0056 0.0018 0.0046 0.0018 0.0001 0.0019 0.0002
Lipid Particles 0.0005 0.0003 0.0005 0.0003 0.0002 0.0002 0.0186 0.0006 0.0045 0.0001 0.0429 0.0095 0.0011 0.0005 0.0023 0.0001 0.0018 0.0003
Mitochondria 0.0001 0.0169 0.0002 0.0003 0.0001 0.0003 0.0118 0.009 0.0006 0.0002 0.0185 0.0157 0.0009 0.0039 0.0054 0.0001 0.0012 0.0002
None 0.3751 0.2397 0.2422 0.2078 0.3295 0.1317 0.2719 0.1681 0.2027 0.0916 0.1155 0.0921 0.4369 0.3417 0.4374 0.3208 0.2232 0.3354
Nuclear Periphery 0.0006 0.0003 0.0009 0.0006 0.0007 0.0003 0.0123 0.0003 0.0007 0.0007 0.0035 0.0023 0.0033 0.0003 0.001 0.0003 0.001 0.0004
Nucleolus 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0011 0.0001 0.0002 0 0.0004 0.0004 0.0001 0.0005 0.0003 0 0.0018 0
Nucleus 0.0005 0.0006 0.0011 0.001 0.0011 0.0006 0.0089 0.0005 0.0012 0.0009 0.0014 0.001 0.0005 0.0016 0.001 0.0004 0.0036 0.0004
Peroxisomes 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0195 0.001 0.004 0 0.0183 0.017 0.0004 0.0113 0.0045 0 0.0013 0.0006
Punctate Nuclear 0.0003 0.0002 0.0006 0.0003 0.0005 0.0002 0.0105 0.0002 0.0011 0.0002 0.0008 0.0024 0.0004 0.003 0.0027 0.0001 0.0056 0.0003
Vacuole 0.0013 0.0026 0.0037 0.0045 0.0034 0.0011 0.0056 0.0013 0.0019 0.0045 0.0186 0.001 0.0017 0.0015 0.0026 0.0017 0.004 0.0009
Vacuole Periphery 0.0001 0.0002 0.0003 0.0006 0.0003 0.0001 0.0016 0.0002 0.0002 0.0008 0.0061 0.0003 0.0005 0.0001 0.0004 0.0002 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.077 17.2594 17.9301 21.0318 20.0261 13.8291 21.8864 22.1824 18.9055 23.4376
Translational Efficiency 0.3403 0.4214 0.3568 0.2758 0.2834 0.4742 0.3313 0.3138 0.2791 0.2629

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
915 736 941 836 1215 1894 1558 596 2130 2630 2499 1432

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.24 682.43 922.02 907.26 736.96 755.94 930.64 866.13 701.43 735.37 927.39 890.14
Standard Deviation 94.71 96.89 137.46 104.59 79.80 94.19 108.85 103.41 95.72 100.53 120.49 106.05
Intensity Change Log 2 — 0.060861 0.494978 0.471696 — 0.036685 0.336637 0.232997 — 0.048105 0.413268 0.350180

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000091 0.000581 0.000260 0.004157 0.000166 0.001170 0.000105 0.000553 0.000134 0.001005 0.000164 0.002657
Bud Neck 0.003600 0.021009 0.001445 0.005588 0.012334 0.039216 0.001149 0.001737 0.008582 0.034121 0.001260 0.003985
Bud Site 0.005568 0.025391 0.008823 0.039184 0.006869 0.041943 0.003175 0.016955 0.006310 0.037311 0.005302 0.029933
Cell Periphery 0.000170 0.000101 0.000100 0.000175 0.000149 0.000129 0.000084 0.000159 0.000158 0.000121 0.000090 0.000168
Cytoplasm 0.697920* 0.482449* 0.740714* 0.521778* 0.552809* 0.446214* 0.678344* 0.639545* 0.615145* 0.456354* 0.701830* 0.570793*
Cytoplasmic Foci 0.127939 0.229502* 0.030023 0.050502 0.240819* 0.239177* 0.028768 0.046560 0.192328* 0.236469* 0.029240 0.048862
Eisosomes 0.000068 0.000088 0.000022 0.000047 0.000211 0.000202 0.000036 0.000042 0.000149 0.000170 0.000031 0.000045
Endoplasmic Reticulum 0.000987 0.000672 0.001568 0.001561 0.000669 0.000546 0.000862 0.000869 0.000806 0.000581 0.001128 0.001273
Endosome 0.004847 0.053560 0.003311 0.010654 0.020758 0.048231 0.003963 0.006382 0.013923 0.049722 0.003717 0.008876
Golgi 0.001367 0.006080 0.000175 0.006487 0.004104 0.012628 0.000068 0.004385 0.002929 0.010796 0.000109 0.005612
Lipid Particles 0.003224 0.004987 0.000902 0.000796 0.005708 0.003337 0.001037 0.000718 0.004641 0.003799 0.000986 0.000764
Mitochondria 0.002222 0.002750 0.000095 0.008129 0.002261 0.007214 0.000157 0.000291 0.002244 0.005965 0.000134 0.004867
Mitotic Spindle 0.000149 0.002007 0.001108 0.079889 0.000915 0.011399 0.005762 0.003733 0.000586 0.008771 0.004009 0.048193
None 0.022833 0.001825 0.002947 0.002532 0.000960 0.000814 0.004419 0.001831 0.010356 0.001097 0.003865 0.002240
Nuclear Periphery 0.000163 0.000206 0.000397 0.000353 0.000228 0.000135 0.000664 0.000257 0.000200 0.000155 0.000563 0.000313
Nuclear Periphery Foci 0.001116 0.000999 0.004800 0.001326 0.000458 0.000305 0.001820 0.001470 0.000741 0.000499 0.002942 0.001386
Nucleolus 0.000845 0.001390 0.000134 0.000153 0.000409 0.000640 0.000203 0.000120 0.000596 0.000850 0.000177 0.000139
Nucleus 0.087251 0.031930 0.110453 0.043665 0.045841 0.016521 0.117169 0.048825 0.063630 0.020833 0.114640 0.045812
Peroxisomes 0.000750 0.009325 0.000236 0.003624 0.002862 0.010222 0.000216 0.000414 0.001955 0.009971 0.000223 0.002288
Vacuole 0.037000 0.122019 0.092251 0.217792* 0.100229 0.116273 0.151891 0.224764* 0.073067 0.117881 0.129434 0.220694*
Vacuole Periphery 0.001890 0.003129 0.000236 0.001607 0.001242 0.003685 0.000108 0.000391 0.001520 0.003530 0.000156 0.001101

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.24 -6.11 -1.71 -0.80 -1.34 -2.42 1.56 -1.55 2.01 -1.92 -2.89 -3.28 -1.86 0.60 -1.67
Bud Neck -8.05 2.65 0.91 8.81 -3.63 -12.79 11.93 9.80 18.88 -0.88 -15.87 11.43 9.10 20.88 -3.38
Bud Site -7.71 -1.51 -3.93 3.13 -2.84 -14.87 4.29 -1.54 11.60 -3.73 -16.56 1.32 -4.22 10.70 -5.08
Cell Periphery 6.59 5.51 5.87 1.59 2.10 1.93 9.67 2.67 1.71 -0.58 4.53 10.96 5.88 2.88 0.50
Cytoplasm 17.19 2.08 21.63 3.46 18.62 11.04 -3.03 2.94 -4.83 5.14 20.80 -0.36 15.93 -0.91 16.06
Cytoplasmic Foci -12.17 20.79 20.25 27.27 -0.37 0.23 39.20 35.42 39.62 -4.06 -8.04 42.21 39.30 49.07 -3.52
Eisosomes -3.29 9.50 6.73 10.11 -4.20 0.28 11.39 11.50 5.48 1.03 -0.88 12.85 12.52 6.11 -1.19
Endoplasmic Reticulum 1.94 -3.85 0.67 -0.93 4.19 0.78 -5.48 -1.25 -1.73 3.67 1.78 -6.17 -0.24 -1.78 4.93
Endosome -12.66 2.81 1.79 12.87 -0.07 -12.35 14.41 12.40 22.17 0.69 -19.15 14.24 12.11 25.83 0.36
Golgi -4.93 9.02 0.00 3.76 -1.49 -10.27 10.66 3.29 10.57 -1.52 -12.35 12.53 2.63 11.46 -2.12
Lipid Particles -1.97 6.58 6.94 4.66 0.65 3.29 6.91 7.26 8.06 2.77 1.63 8.74 9.12 8.81 2.47
Mitochondria -0.65 3.83 0.40 0.85 -1.66 -4.29 2.93 3.00 7.67 0.93 -4.52 4.46 1.54 5.44 -1.59
Mitotic Spindle -1.87 -4.73 -6.44 -5.86 -6.17 -5.85 -3.18 -2.86 2.35 -0.47 -6.23 -3.77 -6.88 -3.31 -5.56
None 7.40 7.19 7.12 -1.92 -0.51 0.36 -6.99 -4.02 -3.36 4.52 7.37 5.66 6.42 -4.61 3.15
Nuclear Periphery -1.45 -13.28 -15.39 -13.34 -6.69 1.10 -9.72 -4.03 -9.73 10.69 0.88 -13.29 -10.67 -16.08 2.02
Nuclear Periphery Foci 0.67 -10.88 -1.70 -2.07 10.46 1.60 -13.32 -8.98 -9.39 2.88 2.87 -15.08 -8.38 -10.20 10.66
Nucleolus -2.07 10.78 10.05 4.52 -2.90 -1.59 5.09 7.24 2.86 4.82 -1.96 11.32 11.96 4.37 1.71
Nucleus 12.03 -6.67 4.07 -8.38 10.62 15.49 -23.13 -7.17 -15.07 12.49 19.95 -20.96 -2.37 -18.86 17.22
Peroxisomes -7.15 4.32 -0.54 6.88 -2.55 -10.14 7.84 7.63 15.79 -1.54 -13.30 8.65 5.22 15.96 -2.78
Vacuole -15.22 -15.42 -33.95 -23.66 -21.44 -3.32 -17.42 -20.08 -18.85 -9.29 -12.51 -23.19 -37.24 -31.15 -20.58
Vacuole Periphery -2.29 4.16 3.59 7.49 -1.42 -5.58 10.28 9.04 8.30 -1.11 -5.43 7.47 6.25 9.88 -2.02
Standard name
Human Ortholog
Description Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sak1

Sak1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sak1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available