Standard name
Description Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.45 0.56 0.59 0.65 0.65 0.76 0.72 0.82 0.78 0.35 0.37 0.48 0 0 0 0 0 0
Endoplasmic Reticulum 0.46 0.45 0.44 0.42 0.34 0.29 0.14 0.15 0.14 0.13 0.62 0.58 0.39 0.66 0.62 0.65 0.67 0.65 0.58
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.2 0.06 0 0 0.09 0.1 0.18 0.09 0.14 0.08 0.13 0.15 0.19 0.35 0.28 0.29 0.3 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 2 0 1 1 0 0 0 0 1 3 2 0 0 0 0 1
Bud 0 0 0 0 1 1 1 1 1 0 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 3 2 3 0 1 0 0 0 2 6 7 0 0 0 0 0 0
Cytoplasm 74 30 79 108 115 145 185 92 113 111 46 60 109 0 1 2 2 1 3
Endoplasmic Reticulum 69 30 61 77 60 64 35 19 19 19 83 94 88 53 114 76 76 129 119
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 2
Golgi 0 1 0 0 0 0 1 1 0 1 1 3 3 1 0 1 0 0 4
Mitochondria 0 0 0 0 0 2 0 0 1 0 0 0 0 1 1 0 1 0 1
Nucleus 0 0 0 0 0 2 0 0 0 0 0 1 0 3 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 0 1 1 0 1 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 19 13 8 5 7 20 24 23 13 20 10 21 33 14 64 32 33 60 70
Unique Cell Count 149 66 140 183 178 222 242 128 138 142 133 162 225 80 183 118 115 200 208
Labelled Cell Count 162 75 155 194 186 237 248 137 148 151 143 187 244 80 183 118 115 200 208


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.1 19.0 19.9 19.1 17.9 17.6 17.6 15.6 17.5 16.5 23.2 23.8 21.7 24.3 25.6 25.7
Std Deviation (1e-4) 4.4 4.0 4.1 3.9 3.9 4.5 4.3 4.4 4.4 4.5 5.3 5.6 4.5 5.9 5.4 5.8
Intensity Change (Log2) 0.07 0.01 -0.08 -0.11 -0.11 -0.28 -0.12 -0.2 0.29 0.33 0.2 0.36 0.43 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 1.9 2.7 2.9 4.9 3.6 5.3 4.7 -1.5 -1.2 0.4
Endoplasmic Reticulum -0.3 -0.5 -1.7 -2.5 -5.5 -4.6 -5.0 -5.1 2.3 1.7 -0.9
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.1 0 -4.0 -2.4 -2.2 -0.3 -2.1 -1.0 -2.5 -1.3 -1.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available