Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication; human homolog can partially complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0 0.06 0.06 0.05 0.08 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.99 0.99 0.93 0.86 0.78 0.8 0.74 0.76 0.63 0.98 0.98 0.98 0.97 0.97 0.97 0.98 0.96 0.92 0.95 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.08 0.15 0.1 0.17 0.1 0.16 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.06 0 0.13 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 0 6 13 19 26 19 21 26 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 1 0 1 2 4 2 1 2 1 3 0 0 1 0 0 0 1 0 1 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 2 0 1 0 4 10 20 17 28 23 20 32 3 5 9 6 1 3 0 0 0 0 0 0
Cytoplasm 662 288 242 220 309 379 374 349 351 274 293 250 457 454 543 106 58 108 651 273 228 210 316 356
Endoplasmic Reticulum 3 4 0 3 7 19 33 66 43 63 37 64 10 4 7 2 1 8 1 3 9 4 8 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 3 7 5 7 7 19 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 0 1 2 5 7 21 28 15 49 61 0 0 0 1 0 0 0 0 0 0 1 0
Nucleus 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 1 0 3 0 0 0 0 0 1 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 1 3 4 3 3 2 2 2 1 1 1 2 0 3 1 2 2 1 2
Unique Cell Count 667 292 243 223 313 407 433 445 439 372 387 394 467 461 553 109 60 111 663 283 248 222 338 379
Labelled Cell Count 670 294 245 224 323 424 457 485 486 407 430 463 472 464 561 116 63 120 663 283 248 222 338 379


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 124.6 147.7 61.7 83.0 61.8 45.9 39.1 35.5 32.1 29.3 26.3 26.4 131.0 123.8 113.4 223.4 216.2 235.6 139.8 145.0 139.0
Std Deviation (1e-4) 26.0 37.6 16.9 17.5 17.6 10.3 9.4 8.3 7.5 6.7 5.9 7.3 28.9 27.2 25.4 68.8 76.0 58.0 31.6 35.9 35.9
Intensity Change (Log2) 0.43 0.0 -0.43 -0.66 -0.8 -0.94 -1.08 -1.23 -1.22 1.08 1.0 0.88 1.86 1.81 1.93 1.18 1.23 1.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.3 2.9 3.5 3.2 3.3 3.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.0 2.7 3.7 3.6 3.2 4.3 0 0 0 0 0 0
Cytoplasm -1.1 -1.1 -3.9 -5.8 -7.6 -7.3 -8.6 -8.2 -10.6 -1.8 -1.3 -1.6 -1.9 -2.0 -1.9
Endoplasmic Reticulum 0 0 3.4 4.4 6.3 5.0 6.8 5.0 6.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.4 4.0 3.2 5.8 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 119.3544 149.6461 137.2452 118.8419 112.367 128.8239 109.5826 146.7863 123.973 116.8828 95.0247 136.4465 105.8056 149.2108 136.1496 125.4913 135.7692 112.4362
Actin 0.0024 0.0003 0.0039 0.0008 0.0069 0.0011 0.013 0.0031 0.0041 0.0289 0.0006 0.0018 0.0199 0.0003 0.027 0.0006 0.0015 0.0111
Bud 0.0011 0.001 0.0011 0.0015 0.0016 0.0013 0.0018 0.0004 0.0037 0.0012 0.0022 0.0013 0.0068 0.0008 0.001 0.0037 0.0072 0.0127
Bud Neck 0.0006 0.0009 0.0012 0.0004 0.0014 0.0042 0.001 0.0006 0.0009 0.0024 0.0008 0.0014 0.002 0.0004 0.0008 0.0007 0.0009 0.0019
Bud Periphery 0.0017 0.0008 0.001 0.0014 0.0013 0.001 0.0017 0.0003 0.0073 0.0007 0.0032 0.0008 0.0095 0.0004 0.0016 0.0017 0.0028 0.0297
Bud Site 0.0004 0.0009 0.0008 0.0005 0.0011 0.0003 0.001 0.0011 0.0053 0.0011 0.0004 0.0002 0.0082 0.0005 0.001 0.0009 0.002 0.0081
Cell Periphery 0.0355 0.0211 0.0228 0.0341 0.0056 0.0028 0.0239 0.0165 0.0353 0.0028 0.0205 0.0035 0.012 0.0157 0.0189 0.0175 0.0055 0.0051
Cytoplasm 0.6409 0.8786 0.7718 0.6707 0.6836 0.788 0.63 0.9205 0.7478 0.6345 0.6125 0.7773 0.6323 0.9271 0.8612 0.6431 0.8823 0.6654
Cytoplasmic Foci 0.0094 0.001 0.004 0.0044 0.0179 0.0034 0.0235 0.001 0.016 0.009 0.0076 0.0093 0.016 0.0009 0.0026 0.0055 0.0032 0.0095
Eisosomes 0.0003 0 0.0003 0.0017 0.0006 0.001 0.0007 0.0002 0.0002 0.0002 0.0012 0 0.0003 0 0.0003 0.0003 0 0.0003
Endoplasmic Reticulum 0.0448 0.0094 0.019 0.006 0.0249 0.0789 0.0511 0.009 0.0175 0.0148 0.03 0.0324 0.0511 0.0081 0.0043 0.001 0.0017 0.0159
Endosome 0.0101 0.0016 0.0088 0.0067 0.04 0.0121 0.0117 0.0017 0.0065 0.0203 0.0085 0.0432 0.0273 0.002 0.0034 0.0037 0.0074 0.0374
Golgi 0.0013 0.0002 0.0016 0.0025 0.014 0.0018 0.003 0.0004 0.0015 0.0166 0.001 0.0205 0.0074 0.0004 0.0026 0.0003 0.0003 0.0155
Lipid Particles 0.0058 0.0014 0.0045 0.0209 0.0261 0.001 0.0099 0.0011 0.0052 0.0043 0.0061 0.0009 0.0113 0.0013 0.0014 0.0064 0.0004 0.0031
Mitochondria 0.0079 0.0004 0.0102 0.0193 0.0217 0.0207 0.008 0.0002 0.0097 0.0035 0.0175 0.0137 0.0196 0.0004 0.0019 0.0532 0.0019 0.0654
None 0.0053 0.0004 0.0032 0.012 0.0066 0.0033 0.0111 0.0002 0.0056 0.001 0.0325 0.0017 0.0113 0.0002 0.0009 0.0026 0.0004 0.002
Nuclear Periphery 0.0282 0.006 0.01 0.0086 0.0175 0.0097 0.0251 0.002 0.0079 0.0066 0.0078 0.0047 0.0283 0.0025 0.0043 0.0053 0.0032 0.0221
Nucleolus 0.0139 0.0011 0.0049 0.0103 0.0078 0.0073 0.0097 0.0001 0.0053 0.0003 0.0056 0.0051 0.0063 0.0007 0.0003 0.0096 0.0011 0.0023
Nucleus 0.0106 0.004 0.0051 0.0079 0.0157 0.0049 0.0093 0.0012 0.0043 0.003 0.0085 0.0031 0.0101 0.0019 0.0028 0.0087 0.0068 0.0084
Peroxisomes 0.0005 0 0.0012 0.0054 0.0122 0.0004 0.0026 0.0001 0.0007 0.0037 0.0007 0.0003 0.0126 0 0.0021 0.0022 0.0002 0.0011
Punctate Nuclear 0.0014 0 0.0007 0.0004 0.0036 0.0003 0.0053 0 0.0008 0.0007 0.0003 0.0001 0.002 0 0.0001 0.0002 0.0001 0.0004
Vacuole 0.1486 0.0656 0.109 0.1356 0.072 0.0431 0.1288 0.0383 0.1007 0.1944 0.187 0.0485 0.083 0.0335 0.0542 0.1853 0.0611 0.0617
Vacuole Periphery 0.0292 0.0052 0.0149 0.049 0.0176 0.0134 0.0278 0.0021 0.0136 0.0501 0.0456 0.03 0.0227 0.003 0.0073 0.0476 0.0099 0.0207

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1037.5067 1197.3511 1345.9447 1305.6399 1416.7034 845.4751 1051.3456 1163.9478 1104.3645 1034.904
Translational Efficiency 1.6121 1.5716 1.4238 1.103 1.4339 2.1403 1.5953 1.3347 1.5578 1.6875

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
141 1313 233 495 1485 1154 2323 1668 1626 2467 2556 2163

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5302.42 7487.07 11247.42 9123.92 5746.66 7237.22 11301.68 9636.63 5708.14 7370.20 11296.73 9519.30
Standard Deviation 1103.30 1664.96 1963.38 2505.92 1390.49 1663.74 2182.24 2294.71 1373.67 1669.05 2163.26 2354.60
Intensity Change Log 2 0.497750 1.084871 0.783003 0.332712 0.975742 0.745805 0.414272 1.029144 0.763776

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000057 0.000160 0.000218 0.000030 0.000057 0.000066 0.000153 0.000035 0.000057 0.000075 0.000168
Bud Neck 0.000600 0.000380 0.006621 0.002797 0.000177 0.000418 0.000422 0.000500 0.000214 0.000398 0.000987 0.001026
Bud Site 0.007569 0.002748 0.003680 0.009977 0.000827 0.001917 0.002394 0.006514 0.001412 0.002359 0.002511 0.007307
Cell Periphery 0.032554 0.013277 0.044029 0.027136 0.014719 0.012567 0.013185 0.040956 0.016265 0.012945 0.015997 0.037793
Cytoplasm 0.355073* 0.732162* 0.263881* 0.492612* 0.727653* 0.733476* 0.705972* 0.678806* 0.695345* 0.732777* 0.665671* 0.636196*
Cytoplasmic Foci 0.030187 0.009803 0.023368 0.009350 0.003949 0.010405 0.005246 0.003955 0.006225 0.010084 0.006898 0.005190
Eisosomes 0.000002 0.000001 0.000012 0.000005 0.000001 0.000001 0.000001 0.000003 0.000001 0.000001 0.000002 0.000003
Endoplasmic Reticulum 0.000963 0.000811 0.000330 0.000316 0.000647 0.000412 0.000620 0.000468 0.000674 0.000625 0.000593 0.000433
Endosome 0.001883 0.000559 0.000902 0.001210 0.000324 0.000467 0.000382 0.000353 0.000459 0.000516 0.000429 0.000549
Golgi 0.199173* 0.062250 0.065556 0.034061 0.045501 0.058356 0.027918 0.021079 0.058827 0.060428 0.031349 0.024050
Lipid Particles 0.023687 0.009864 0.028859 0.020029 0.010209 0.008488 0.010566 0.018135 0.011378 0.009220 0.012234 0.018569
Mitochondria 0.000088 0.000222 0.000191 0.000424 0.000117 0.000188 0.000301 0.000337 0.000115 0.000206 0.000291 0.000357
Mitotic Spindle 0.008016 0.000826 0.002543 0.001299 0.000332 0.001178 0.000453 0.000350 0.000998 0.000991 0.000644 0.000567
None 0.000955 0.001921 0.001656 0.001773 0.002286 0.002620 0.001668 0.001459 0.002171 0.002248 0.001667 0.001531
Nuclear Periphery 0.000911 0.000619 0.005452 0.001067 0.000603 0.000653 0.000504 0.000381 0.000629 0.000635 0.000955 0.000538
Nuclear Periphery Foci 0.014520 0.011998 0.023093 0.010146 0.012120 0.012637 0.006631 0.005390 0.012328 0.012297 0.008132 0.006478
Nucleolus 0.000056 0.000066 0.000200 0.000393 0.000085 0.000091 0.000085 0.000123 0.000083 0.000078 0.000096 0.000185
Nucleus 0.002082 0.005212 0.020882 0.035108 0.006861 0.012112 0.006304 0.008255 0.006447 0.008440 0.007633 0.014400
Peroxisomes 0.000031 0.000021 0.000026 0.000069 0.000013 0.000008 0.000035 0.000087 0.000015 0.000015 0.000034 0.000083
Vacuole 0.125613 0.062941 0.370423* 0.273700* 0.084855 0.079165 0.146078 0.168429 0.088389 0.070530 0.166529 0.192520
Vacuole Periphery 0.195947* 0.084262 0.138135* 0.078311 0.088691 0.064783 0.071167 0.044266 0.097992 0.075150 0.077272 0.052057

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.55 -3.58 -3.41 -4.44 -1.23 -3.72 -5.13 -6.71 -4.72 -4.21 -4.23 -5.99 -8.10 -6.49 -5.05
Bud Neck 1.29 -6.86 -7.99 -8.67 3.76 -4.95 -6.99 -9.15 -0.68 -0.08 -3.60 -8.47 -10.80 -7.63 -0.37
Bud Site 2.42 1.68 -1.06 -3.69 -2.96 -4.13 -8.96 -8.98 -6.23 -6.12 -3.91 -5.14 -8.62 -6.92 -6.84
Cell Periphery 3.35 -1.43 1.59 -4.17 4.30 1.52 1.27 -12.11 -13.00 -13.55 2.62 0.33 -10.91 -13.85 -12.01
Cytoplasm -11.96 2.89 -3.88 13.64 -9.33 -0.20 2.80 4.38 4.37 1.94 -3.32 3.52 5.52 9.99 2.29
Cytoplasmic Foci 5.56 0.27 4.96 -1.01 4.41 -8.05 -3.62 -0.49 7.84 3.26 -5.93 -2.36 0.80 7.07 3.23
Eisosomes 2.01 -5.41 -3.68 -5.20 3.57 -2.84 -3.69 -6.64 -4.50 -5.12 -2.93 -6.27 -8.42 -6.50 -3.25
Endoplasmic Reticulum 0.59 2.86 3.07 4.11 0.26 2.38 0.40 2.17 -0.68 1.96 0.50 1.02 3.23 2.56 2.41
Endosome 4.71 3.09 1.54 -1.40 -0.41 -3.17 -2.36 -0.90 2.73 1.55 -1.46 0.42 -0.71 0.01 -0.90
Golgi 9.71 9.67 12.20 9.20 7.33 -3.83 8.02 11.17 13.28 4.99 -0.50 10.76 14.01 17.47 6.08
Lipid Particles 3.22 -1.29 1.17 -3.88 3.75 1.35 -0.49 -5.37 -6.78 -5.61 1.95 -1.02 -4.98 -7.74 -4.77
Mitochondria -4.58 -4.72 -2.61 -1.61 -1.72 -2.18 -3.91 -3.42 -1.65 -0.38 -3.79 -4.15 -4.19 -2.30 -0.91
Mitotic Spindle 1.26 0.87 1.15 -1.33 2.17 -3.35 -3.03 -0.42 3.29 2.87 0.02 0.57 0.78 2.35 0.88
None -5.56 -3.09 -2.87 0.09 -0.91 -0.80 1.64 2.14 2.13 0.44 -0.39 1.48 1.92 1.98 0.25
Nuclear Periphery 2.10 -7.68 -1.03 -2.66 7.15 -0.94 1.22 4.80 5.58 4.39 -0.20 -4.89 1.18 1.44 5.54
Nuclear Periphery Foci 1.20 -3.30 2.10 1.58 6.16 -0.53 6.90 8.36 8.74 2.95 -0.01 5.39 7.64 8.88 3.69
Nucleolus -0.77 -9.70 -4.73 -4.50 -2.62 -0.56 -0.19 -1.55 -1.64 -5.13 -0.05 -0.89 -3.92 -5.13 -4.78
Nucleus -4.22 -7.27 -9.56 -8.95 -4.68 -4.23 -0.95 -2.34 2.47 -1.67 -2.39 -2.85 -8.12 -7.06 -6.56
Peroxisomes 1.22 0.37 -2.08 -3.00 -2.43 1.48 -4.26 -9.37 -10.51 -6.04 -0.19 -4.13 -9.49 -9.79 -6.17
Vacuole 4.53 -12.15 -8.45 -16.78 5.09 0.54 -11.43 -12.99 -13.02 -2.59 3.52 -14.51 -16.89 -21.54 -3.16
Vacuole Periphery 6.18 3.55 7.09 2.83 5.99 4.89 4.20 10.80 5.45 8.93 4.98 5.10 11.62 8.11 9.05