Standard name
Description GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0 0.1 0.07 0 0.06 0.06 0.09 0.05 0.08 0.1 0.08 0 0.09 0.1 0 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.94 0.94 0.93 0.87 0.87 0.9 0.9 0.83 0.99 1.0 0.98 0.96 0.99 0.91 0.98 0.93 0.91 0.95 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0.06 0.05 0.12 0.1 0.07 0.07 0.11 0 0 0 0.07 0 0 0 0 0.05 0 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 1 3 3 5 2 10 0 0 0 0 1 0 0 0 2 0 2 2
Bud 1 0 3 1 1 0 4 4 3 3 3 2 2 2 0 1 1 0 0 2 0 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 13 1 34 16 9 20 29 29 20 29 47 22 8 30 14 9 10 0 0 1 0 1 1
Cytoplasm 268 128 330 219 321 321 442 280 398 340 389 279 200 330 132 213 117 269 119 325 191 458 419
Endoplasmic Reticulum 4 0 12 8 19 17 60 32 30 25 51 5 1 3 9 7 4 2 0 18 6 27 22
Endosome 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 0 6
Golgi 0 0 1 1 1 0 1 0 2 0 2 0 0 0 1 0 4 0 0 0 0 0 0
Mitochondria 0 2 0 0 0 0 4 1 5 3 9 0 0 0 2 2 1 0 1 0 0 0 2
Nucleus 0 0 0 1 1 1 0 2 2 1 1 0 0 1 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 2 0 0 1 2 0 0 2 0 0 0 0 0 0 1 0 1 2 1
Unique Cell Count 273 130 342 232 340 344 507 322 443 379 471 283 200 336 138 215 128 276 128 358 203 499 471
Labelled Cell Count 287 131 383 246 354 360 543 352 468 404 512 310 211 366 158 234 140 276 128 358 203 499 471


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.7 23.8 29.4 29.3 28.6 26.6 25.5 24.9 22.1 21.7 24.3 26.0 24.9 30.0 19.6 22.8 27.3 28.6 31.4 32.8
Std Deviation (1e-4) 4.5 6.7 5.1 4.9 5.4 4.4 5.3 5.3 4.7 4.5 5.5 4.5 5.3 5.5 4.6 6.2 7.9 4.7 5.6 7.1
Intensity Change (Log2) -0.0 -0.04 -0.14 -0.21 -0.24 -0.41 -0.44 -0.27 -0.18 -0.24 0.03 -0.58 -0.37 -0.1 -0.04 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 1.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -3.9 -2.0 -2.3 -0.4 -3.0 -1.1 0 -0.9 -2.5 -0.5 0.1 -2.5 -0.7
Cytoplasm -1.2 -1.3 -1.9 -4.6 -4.5 -3.6 -3.5 -6.1 1.7 2.7 1.4 -0.4 1.9 -2.3
Endoplasmic Reticulum 0 1.3 0.9 4.3 3.3 2.0 1.9 3.9 -1.3 0 -2.3 1.5 -0.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gdi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available