Standard name
Description High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.11 0.17 0.16 0.31 0.42 0.26 0.11 – 0.33 0.4 0.33 0.18 0.13 0.1 0.06 0.07 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0.07 0.05 0.07 – 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.95 0.91 0.88 0.9 0.69 0.63 0.56 0.36 – 0.65 0.69 0.66 0.95 0.92 0.93 0.86 0.85 0.87 0.95 0.91 0.94 0.9 0.89 0.89
Cytoplasm 0 0.06 0.08 0.05 0.12 0.1 0.1 0.11 – 0.11 0.06 0.06 0 0 0.05 0.08 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.07 – 0.07 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.17 0.14 0.26 0.35 0.37 0.35 – 0.16 0.35 0.32 0 0 0 0.17 0.1 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.09 – 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 27 36 87 71 163 289 161 50 – 187 226 220 53 34 26 20 22 13 5 7 6 4 4 9
Bud 4 0 4 5 22 45 30 32 – 47 23 24 3 0 0 1 0 0 5 7 7 4 3 8
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 2 1 0 1
Bud Site 0 0 0 1 5 11 6 0 – 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 467 292 444 396 361 436 339 166 – 371 397 435 284 236 252 289 253 212 530 389 554 352 236 287
Cytoplasm 10 18 42 20 65 68 59 52 – 60 36 39 12 10 14 26 28 23 2 4 4 3 2 4
Endoplasmic Reticulum 16 9 10 8 17 22 36 31 – 42 22 31 2 11 3 11 10 12 4 5 4 4 5 1
Endosome 0 0 1 0 0 0 0 0 – 0 0 0 0 2 0 1 0 2 0 1 0 1 0 0
Golgi 1 6 0 0 0 0 0 7 – 2 0 0 1 0 3 6 10 5 0 0 0 0 1 0
Mitochondria 11 17 87 60 137 243 223 159 – 92 198 213 1 0 0 58 30 26 3 6 10 15 6 7
Nucleus 0 3 2 2 3 5 5 3 – 2 0 2 1 0 0 2 2 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 8 2 8 20 41 – 27 4 32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 2 0 12 – 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 19 11 5 0 – 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0
SpindlePole 1 0 1 1 18 14 8 2 – 3 2 2 1 0 0 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 1 0 10 1 2 23 – 7 3 2 1 1 2 9 0 2 1 2 0 0 1 0
Unique Cell Count 492 322 504 440 525 691 608 460 569 572 659 300 256 271 338 296 245 556 428 593 392 264 324
Labelled Cell Count 540 382 679 572 822 1155 894 578 844 912 1001 359 294 300 425 357 298 556 428 593 392 264 324


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.5 25.7 22.7 22.5 17.0 15.7 16.5 18.4 – 20.6 22.3 21.6 32.6 36.2 39.6 26.5 26.6 27.9 23.9 24.1 23.4
Std Deviation (1e-4) 6.0 8.3 6.1 5.6 6.5 5.6 5.4 5.1 – 6.0 7.4 6.6 7.6 9.1 10.2 9.3 9.5 10.0 6.3 7.0 6.6
Intensity Change (Log2) – – – -0.02 -0.42 -0.53 -0.46 -0.31 – -0.15 -0.03 -0.08 0.52 0.67 0.8 0.22 0.22 0.3 0.07 0.08 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.5 5.2 9.0 3.7 -2.8 – 5.8 8.0 6.2 0.1 -1.4 -2.9 -4.8 -3.9 -4.5
Bud 0 3.5 4.9 4.0 5.0 – 5.7 3.4 3.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 -7.5 -9.7 -11.8 -16.7 – -8.8 -7.4 -8.7 3.1 1.7 2.1 -1.1 -1.1 -0.6
Cytoplasm -2.3 2.1 0.9 0.8 1.6 – 1.2 -1.3 -1.6 -2.4 -2.3 -1.6 -0.3 0.5 0.5
Endoplasmic Reticulum -0.2 1.3 1.3 3.3 3.7 – 4.1 1.8 2.5 0 1.8 0 1.2 1.2 2.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -1.5 3.4 6.8 7.2 6.2 – -0.5 6.4 5.8 -7.4 -7.1 -7.3 0 -2.8 -2.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 4.1 6.8 – 5.0 0 5.0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.6 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 4.3 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.8 2.8 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 0 0 4.8 – 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ftr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available