Standard name | |
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Description | High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 27 | 36 | 87 | 71 | 163 | 289 | 161 | 50 | – | 187 | 226 | 220 | 53 | 34 | 26 | 20 | 22 | 13 | 5 | 7 | 6 | 4 | 4 | 9 |
Bud | 4 | 0 | 4 | 5 | 22 | 45 | 30 | 32 | – | 47 | 23 | 24 | 3 | 0 | 0 | 1 | 0 | 0 | 5 | 7 | 7 | 4 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 5 | 11 | 6 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 467 | 292 | 444 | 396 | 361 | 436 | 339 | 166 | – | 371 | 397 | 435 | 284 | 236 | 252 | 289 | 253 | 212 | 530 | 389 | 554 | 352 | 236 | 287 |
Cytoplasm | 10 | 18 | 42 | 20 | 65 | 68 | 59 | 52 | – | 60 | 36 | 39 | 12 | 10 | 14 | 26 | 28 | 23 | 2 | 4 | 4 | 3 | 2 | 4 |
Endoplasmic Reticulum | 16 | 9 | 10 | 8 | 17 | 22 | 36 | 31 | – | 42 | 22 | 31 | 2 | 11 | 3 | 11 | 10 | 12 | 4 | 5 | 4 | 4 | 5 | 1 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 |
Golgi | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 7 | – | 2 | 0 | 0 | 1 | 0 | 3 | 6 | 10 | 5 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 11 | 17 | 87 | 60 | 137 | 243 | 223 | 159 | – | 92 | 198 | 213 | 1 | 0 | 0 | 58 | 30 | 26 | 3 | 6 | 10 | 15 | 6 | 7 |
Nucleus | 0 | 3 | 2 | 2 | 3 | 5 | 5 | 3 | – | 2 | 0 | 2 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 8 | 2 | 8 | 20 | 41 | – | 27 | 4 | 32 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 12 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 19 | 11 | 5 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 1 | 18 | 14 | 8 | 2 | – | 3 | 2 | 2 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 1 | 0 | 10 | 1 | 2 | 23 | – | 7 | 3 | 2 | 1 | 1 | 2 | 9 | 0 | 2 | 1 | 2 | 0 | 0 | 1 | 0 |
Unique Cell Count | 492 | 322 | 504 | 440 | 525 | 691 | 608 | 460 | 569 | 572 | 659 | 300 | 256 | 271 | 338 | 296 | 245 | 556 | 428 | 593 | 392 | 264 | 324 | |
Labelled Cell Count | 540 | 382 | 679 | 572 | 822 | 1155 | 894 | 578 | 844 | 912 | 1001 | 359 | 294 | 300 | 425 | 357 | 298 | 556 | 428 | 593 | 392 | 264 | 324 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 24.5 | 25.7 | 22.7 | 22.5 | 17.0 | 15.7 | 16.5 | 18.4 | – | 20.6 | 22.3 | 21.6 | 32.6 | 36.2 | 39.6 | 26.5 | 26.6 | 27.9 | 23.9 | 24.1 | 23.4 |
Std Deviation (1e-4) | 6.0 | 8.3 | 6.1 | 5.6 | 6.5 | 5.6 | 5.4 | 5.1 | – | 6.0 | 7.4 | 6.6 | 7.6 | 9.1 | 10.2 | 9.3 | 9.5 | 10.0 | 6.3 | 7.0 | 6.6 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.42 | -0.53 | -0.46 | -0.31 | – | -0.15 | -0.03 | -0.08 | 0.52 | 0.67 | 0.8 | 0.22 | 0.22 | 0.3 | 0.07 | 0.08 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | -0.5 | 5.2 | 9.0 | 3.7 | -2.8 | – | 5.8 | 8.0 | 6.2 | 0.1 | -1.4 | -2.9 | -4.8 | -3.9 | -4.5 |
Bud | 0 | 3.5 | 4.9 | 4.0 | 5.0 | – | 5.7 | 3.4 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | -7.5 | -9.7 | -11.8 | -16.7 | – | -8.8 | -7.4 | -8.7 | 3.1 | 1.7 | 2.1 | -1.1 | -1.1 | -0.6 |
Cytoplasm | -2.3 | 2.1 | 0.9 | 0.8 | 1.6 | – | 1.2 | -1.3 | -1.6 | -2.4 | -2.3 | -1.6 | -0.3 | 0.5 | 0.5 |
Endoplasmic Reticulum | -0.2 | 1.3 | 1.3 | 3.3 | 3.7 | – | 4.1 | 1.8 | 2.5 | 0 | 1.8 | 0 | 1.2 | 1.2 | 2.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.5 | 3.4 | 6.8 | 7.2 | 6.2 | – | -0.5 | 6.4 | 5.8 | -7.4 | -7.1 | -7.3 | 0 | -2.8 | -2.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 4.1 | 6.8 | – | 5.0 | 0 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3.6 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 4.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.8 | 2.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.7 | 0 | 0 | 4.8 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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