Standard name
Human Ortholog
Description Ubiquitin-specific protease involved in transport and osmotic response; negatively regulates Ras/PKA signaling; interacts with Bre5p to coregulate anterograde, retrograde transport between ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; role in ribophagy; cleaves ubiquitin fusions but not polyubiquitin; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0.07 0 0.07 0 0.05 0 0 0.06 0.06 0 0 0 0 0.09 0 0 0 0 0 0 0
Cytoplasm 0.97 0.95 0.92 0.87 0.87 0.78 0.7 0.66 0.66 0.58 0.55 0.55 0.97 0.95 0.95 0.88 0.92 0.93 0.93 0.95 0.88 0.71 0.61 0.54
Endoplasmic Reticulum 0 0.05 0.08 0.11 0.1 0.17 0.17 0.23 0.18 0.17 0.14 0.15 0 0 0 0 0 0 0 0 0.05 0.17 0.24 0.26
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.12 0 0.16 0.23 0.32 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0.1 0 0 0 0.11 0 0.05 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 2 10 12 14 7 11 17 18 0 0 1 2 2 0 0 0 0 1 3 1
Bud 0 0 1 0 0 0 3 2 2 1 2 1 0 0 0 0 0 0 0 4 1 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4 19 22 17 14 24 15 18 10 12 17 14 6 8 11 17 31 12 0 0 0 0 0 0
Cytoplasm 296 498 439 218 278 269 247 230 153 159 151 136 271 290 327 369 314 342 277 501 438 147 245 155
Endoplasmic Reticulum 8 26 37 27 33 59 60 79 42 46 39 38 5 13 15 13 12 12 9 7 25 35 97 73
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 1 0 0 1 2 6
Golgi 0 0 1 1 1 3 5 8 2 1 0 4 0 0 1 8 2 1 0 0 3 2 12 10
Mitochondria 0 1 0 1 2 15 42 15 37 63 87 57 2 1 0 5 0 2 0 2 6 3 9 4
Nucleus 0 0 2 0 1 0 3 5 5 3 2 3 2 1 2 1 5 4 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 1 0 0 0 2 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 2 2 3 1 6 5 13 16 11 15 8 25 0 0 1 48 13 17 2 9 15 8 17 21
Unique Cell Count 305 522 476 250 318 346 353 351 231 272 273 246 280 305 343 420 342 369 300 531 497 207 401 285
Labelled Cell Count 310 547 506 266 338 385 400 389 270 311 324 297 286 313 358 464 380 391 300 531 497 207 401 285


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.8 17.1 13.5 12.5 13.5 11.8 11.7 12.0 11.0 10.2 9.2 10.0 15.3 16.3 16.4 16.4 18.0 17.1 14.1 15.1 16.0
Std Deviation (1e-4) 3.0 2.8 1.8 1.7 1.6 1.9 1.9 1.8 2.0 1.8 1.9 1.9 2.4 2.3 2.6 4.0 3.7 3.8 2.8 2.5 3.0
Intensity Change (Log2) -0.11 0.0 -0.19 -0.2 -0.17 -0.29 -0.4 -0.55 -0.43 0.19 0.27 0.29 0.28 0.42 0.35 0.07 0.17 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.7 4.0 0 0 5.2 5.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.2 -0.1 1.4 -0.3 0.3 -0.2 -0.1 1.0 0.6 -1.7 -1.4 -1.0 -0.4 2.5 -1.0
Cytoplasm -2.2 -2.2 -5.9 -8.4 -9.7 -8.8 -11.1 -11.9 -11.7 2.5 1.6 1.8 -2.2 -0.2 0.2
Endoplasmic Reticulum 1.4 1.3 4.1 4.1 6.0 4.1 3.8 2.8 3.2 -3.5 -2.0 -2.0 -3.0 -2.5 -2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 7.7 4.6 9.0 11.0 13.1 10.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.2 3.7 0 4.2 0 6.3 0 0 0 7.0 3.2 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.1607 15.734 13.4232 11.5687 11.1686 13.6283 11.7596 16.1865 14.7565 12.2784 12.6342 14.516 10.8645 14.3946 13.2516 13.2177 11.5316 13.0438
Actin 0.0094 0.0023 0.0055 0.0235 0.0199 0.0073 0.0148 0.0006 0.0016 0.0229 0.0032 0.0014 0.0193 0.0043 0.0104 0.0323 0.0298 0.0055
Bud 0.001 0.0006 0.0014 0.0015 0.0014 0.001 0.0025 0.0033 0.0014 0.0029 0.0006 0.001 0.0098 0.0119 0.0145 0.0117 0.0072 0.005
Bud Neck 0.0036 0.0006 0.001 0.0014 0.0015 0.0035 0.0018 0.0004 0.0007 0.0014 0.0006 0.0011 0.0011 0.0007 0.0011 0.0012 0.001 0.002
Bud Periphery 0.0012 0.0004 0.0013 0.0012 0.0018 0.001 0.0022 0.0008 0.0006 0.0036 0.0004 0.001 0.0057 0.0038 0.006 0.0069 0.0028 0.0018
Bud Site 0.0042 0.0016 0.0043 0.0021 0.0139 0.001 0.0062 0.0068 0.0011 0.0078 0.0006 0.0002 0.0093 0.0415 0.0158 0.0082 0.0014 0.0033
Cell Periphery 0.002 0.0012 0.0013 0.0006 0.0005 0.0005 0.0012 0.0003 0.0004 0.0013 0.0001 0.0003 0.0015 0.001 0.0009 0.0007 0.0004 0.0004
Cytoplasm 0.6016 0.918 0.7614 0.6415 0.549 0.7196 0.7055 0.9028 0.863 0.6056 0.6994 0.7974 0.7169 0.8475 0.8207 0.7941 0.6896 0.8001
Cytoplasmic Foci 0.023 0.0096 0.0215 0.0255 0.0341 0.033 0.0183 0.0135 0.0198 0.0238 0.0357 0.0218 0.0305 0.0299 0.0221 0.0359 0.0362 0.0305
Eisosomes 0.0003 0 0.0005 0.0002 0.0003 0 0.0004 0 0 0.0002 0.0001 0 0.0002 0 0 0.0002 0.0002 0
Endoplasmic Reticulum 0.1374 0.0226 0.0343 0.0416 0.0606 0.078 0.0771 0.0155 0.0248 0.0271 0.0119 0.0444 0.0352 0.0108 0.0163 0.0061 0.007 0.0346
Endosome 0.1034 0.0242 0.078 0.0848 0.1462 0.0847 0.0821 0.0339 0.0508 0.1564 0.1682 0.0825 0.0714 0.0247 0.0468 0.0475 0.1492 0.0752
Golgi 0.0188 0.0076 0.0116 0.0389 0.0277 0.0185 0.0173 0.006 0.0085 0.0256 0.0205 0.0189 0.0105 0.0031 0.0069 0.0097 0.0116 0.01
Lipid Particles 0.0088 0.0002 0.0071 0.0078 0.03 0.0006 0.004 0.0001 0.0003 0.0048 0.004 0.0001 0.0046 0.0009 0.0007 0.0147 0.0024 0.0008
Mitochondria 0.0172 0.0024 0.0126 0.0414 0.0324 0.0269 0.0274 0.0026 0.0041 0.0542 0.0133 0.0177 0.0188 0.002 0.005 0.0016 0.0021 0.0086
None 0.0036 0.0005 0.0026 0.0006 0.0007 0.0011 0.0019 0.0005 0.0008 0.0015 0.002 0.0007 0.0109 0.0035 0.003 0.0064 0.0066 0.0022
Nuclear Periphery 0.0116 0.0011 0.004 0.0044 0.007 0.0035 0.0069 0.0008 0.0013 0.0032 0.0152 0.001 0.0076 0.001 0.0022 0.0017 0.003 0.0041
Nucleolus 0.001 0 0.0008 0.0001 0.0004 0.0009 0.0003 0 0 0.0003 0.0003 0 0.0038 0.0005 0.0028 0.0002 0.0003 0.0003
Nucleus 0.0059 0.0009 0.0075 0.0015 0.0026 0.0021 0.0028 0.0008 0.0009 0.0019 0.0026 0.0007 0.0079 0.0031 0.0041 0.0017 0.0033 0.0029
Peroxisomes 0.0018 0.0001 0.0037 0.0286 0.0384 0.0002 0.0014 0.0001 0.0002 0.0064 0.0009 0.0001 0.0013 0.0005 0.0005 0.0092 0.0029 0.0004
Punctate Nuclear 0.0056 0.0001 0.0005 0.0003 0.0011 0.0006 0.0007 0.0001 0.0001 0.0016 0.0007 0 0.0038 0.0009 0.0008 0.0027 0.0026 0.0028
Vacuole 0.0319 0.0047 0.0318 0.0417 0.019 0.0114 0.0195 0.0091 0.016 0.0331 0.0124 0.006 0.0253 0.007 0.0164 0.0056 0.0332 0.0069
Vacuole Periphery 0.0066 0.0012 0.0072 0.0109 0.0112 0.0045 0.0059 0.0019 0.0037 0.0145 0.0073 0.0037 0.0045 0.0014 0.003 0.0017 0.0073 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.4864 33.8515 25.6538 42.8157 43.8742 30.5996 49.1562 51.9775 38.1641 50.9308
Translational Efficiency 1.3224 1.6973 1.6755 0.8687 1.0396 1.4217 1.1567 0.8779 1.0431 0.9098

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1744 1738 474 1938 2381 2187 592 1129 4125 3925 1066 3067

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1267.36 1495.68 1853.05 1587.81 1334.52 1377.30 1597.42 1478.10 1306.13 1429.72 1711.09 1547.42
Standard Deviation 186.04 241.15 241.96 211.76 212.27 206.83 204.03 248.49 204.31 230.31 255.51 232.09
Intensity Change Log 2 0.238975 0.548075 0.325212 0.045522 0.259423 0.147423 0.142994 0.407238 0.236761

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000219 0.000183 0.000366 0.000254 0.000091 0.000108 0.000428 0.000449 0.000145 0.000141 0.000400 0.000326
Bud Neck 0.000158 0.000220 0.003168 0.000901 0.000120 0.000150 0.002026 0.001162 0.000136 0.000181 0.002534 0.000997
Bud Site 0.021109 0.016186 0.021168 0.020373 0.013299 0.013243 0.024843 0.042052 0.016601 0.014546 0.023209 0.028353
Cell Periphery 0.000565 0.000569 0.001679 0.001954 0.000590 0.000361 0.001025 0.001364 0.000579 0.000453 0.001315 0.001737
Cytoplasm 0.677910* 0.760681* 0.647127* 0.797180* 0.730529* 0.742739* 0.732085* 0.694988* 0.708282* 0.750684* 0.694308* 0.759562*
Cytoplasmic Foci 0.197320* 0.122394 0.047997 0.033270 0.143116 0.149014 0.062726 0.080244 0.166033 0.137226 0.056176 0.050562
Eisosomes 0.000003 0.000003 0.000003 0.000003 0.000002 0.000002 0.000004 0.000004 0.000003 0.000003 0.000004 0.000004
Endoplasmic Reticulum 0.009229 0.006962 0.002732 0.001163 0.006860 0.003940 0.004907 0.001916 0.007861 0.005278 0.003940 0.001440
Endosome 0.005499 0.005439 0.014358 0.004992 0.004247 0.006303 0.014456 0.011548 0.004776 0.005921 0.014413 0.007405
Golgi 0.032728 0.037431 0.044701 0.019795 0.029185 0.035667 0.025520 0.033976 0.030683 0.036448 0.034049 0.025015
Lipid Particles 0.000654 0.001111 0.005566 0.006596 0.000779 0.000673 0.007489 0.006997 0.000726 0.000867 0.006634 0.006744
Mitochondria 0.000811 0.000495 0.000089 0.000264 0.000963 0.000879 0.000042 0.000185 0.000899 0.000709 0.000063 0.000235
Mitotic Spindle 0.001386 0.003025 0.025112 0.001589 0.000971 0.001721 0.005578 0.002815 0.001147 0.002298 0.014264 0.002040
None 0.000286 0.000528 0.000359 0.000111 0.001052 0.000250 0.000245 0.000107 0.000728 0.000373 0.000296 0.000109
Nuclear Periphery 0.000025 0.000042 0.001030 0.000024 0.000030 0.000032 0.000145 0.000030 0.000028 0.000036 0.000538 0.000026
Nuclear Periphery Foci 0.000792 0.001618 0.016857 0.001443 0.000756 0.000781 0.007333 0.001940 0.000772 0.001151 0.011568 0.001626
Nucleolus 0.000017 0.000025 0.000089 0.000108 0.000022 0.000025 0.000053 0.000061 0.000020 0.000025 0.000069 0.000091
Nucleus 0.000648 0.000758 0.004329 0.001912 0.001091 0.001047 0.002968 0.001571 0.000903 0.000919 0.003573 0.001787
Peroxisomes 0.000120 0.000118 0.000144 0.000459 0.000108 0.000128 0.000249 0.000700 0.000113 0.000124 0.000202 0.000548
Vacuole 0.010119 0.013236 0.108356 0.088398 0.011936 0.015019 0.092967 0.094294 0.011168 0.014230 0.099810 0.090569
Vacuole Periphery 0.040403 0.028975 0.054770 0.019210 0.054254 0.027916 0.014911 0.023595 0.048398 0.028385 0.032635 0.020824

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.59 -1.78 -0.74 -2.69 1.62 -1.40 -8.04 -7.66 -7.19 -0.29 0.14 -5.85 -5.95 -8.06 1.75
Bud Neck -2.80 -7.75 -11.24 -10.16 5.84 -2.30 -8.91 -12.53 -12.19 3.66 -3.68 -11.30 -16.63 -15.72 7.10
Bud Site 4.02 -0.22 0.32 -3.57 0.39 0.05 -5.32 -10.65 -10.78 -5.02 2.81 -4.13 -9.27 -11.20 -2.51
Cell Periphery -0.09 -4.27 -5.07 -5.03 -0.68 2.41 -3.47 -4.14 -5.11 -1.53 2.18 -5.42 -6.17 -6.73 -1.79
Cytoplasm -8.67 2.41 -13.12 -4.06 -10.26 -1.51 0.24 3.92 5.11 2.49 -6.87 1.96 -7.65 -0.99 -6.73
Cytoplasmic Foci 11.71 25.90 31.09 21.39 5.00 -1.12 16.66 13.61 14.17 -3.48 7.06 29.58 34.50 27.62 2.53
Eisosomes 1.08 0.31 -0.17 -1.23 -0.45 -0.03 -5.78 -6.29 -5.95 0.31 0.81 -4.19 -4.76 -4.47 0.66
Endoplasmic Reticulum 1.83 6.10 9.75 6.18 2.20 4.23 1.50 8.24 4.18 2.41 3.87 4.51 13.26 7.85 3.30
Endosome 0.12 -7.15 1.11 0.89 7.40 -5.71 -8.94 -11.13 -7.44 2.45 -4.23 -11.48 -7.41 -3.75 8.04
Golgi -2.58 -3.49 8.38 9.88 7.64 -4.12 1.85 -1.78 1.32 -2.85 -4.84 -1.47 5.25 8.86 4.53
Lipid Particles -4.33 -7.12 -12.47 -11.34 -1.02 1.23 -9.10 -8.50 -8.65 0.52 -2.10 -11.54 -14.88 -14.49 -0.01
Mitochondria 2.46 6.41 4.52 2.95 -3.36 0.32 13.98 9.60 2.80 -2.86 1.25 13.70 9.49 3.34 -4.61
Mitotic Spindle -2.32 -7.08 -0.64 1.68 6.95 -3.52 -5.27 -3.10 -2.02 2.34 -3.44 -8.26 -2.79 0.11 7.43
None -1.21 -1.98 7.95 2.08 7.03 2.41 2.42 2.84 6.05 2.93 1.68 2.22 3.22 2.95 6.33
Nuclear Periphery -1.75 -3.02 -0.14 1.77 3.02 -0.56 -3.93 -1.11 -0.19 3.83 -1.78 -3.44 -0.28 1.70 3.44
Nuclear Periphery Foci -3.78 -8.81 -5.49 0.70 8.42 -0.24 -7.78 -8.29 -7.63 6.28 -3.35 -11.41 -9.52 -3.81 10.44
Nucleolus -1.15 -5.29 -16.60 -10.54 -0.62 -1.11 -5.08 -10.42 -9.15 -1.34 -1.46 -6.99 -20.43 -15.17 -2.21
Nucleus -2.22 -4.23 -10.75 -9.84 2.86 0.17 -6.13 -3.14 -3.83 4.17 -0.16 -6.16 -7.57 -8.52 4.04
Peroxisomes 0.10 -1.52 -10.76 -10.89 -9.06 -1.68 -6.08 -8.18 -7.92 -5.90 -1.15 -5.88 -13.14 -12.90 -9.59
Vacuole -2.40 -12.94 -24.30 -23.07 2.61 -2.94 -12.17 -19.05 -18.26 -0.44 -3.74 -17.70 -30.90 -29.45 1.64
Vacuole Periphery 4.31 -2.74 9.60 4.98 7.79 9.91 13.23 11.36 2.52 -3.32 10.49 5.95 15.62 5.87 4.78