Standard name
Human Ortholog
Description Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0 0 0.05 0.07 0.07
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0.09 0 0.09 0 0 0 0 0 0 0 0 0.21 0.24 0.38 0.29 0.28 0.25 0.08 0.08 0 0.08 0.1 0.09
Mitochondria 0.87 0.86 0.94 0.95 0.89 0.9 0.9 0.9 0.96 0.91 0.95 0.96 0.82 0.79 0.73 0.12 0.1 0.09 0.82 0.8 0.87 0.7 0.67 0.67
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.07 0 0.06 0.1 0.11 0.08 0.1 0.1 0.1 0.09 0 0 0 0.07 0.08 0.14 0 0 0 0 0 0
Nucleolus 0.08 0 0.05 0 0.07 0.11 0.12 0.1 0.09 0.08 0.08 0.07 0 0 0 0.08 0.07 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.12 0.12 0 0 0 0.06 0.05 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.13 0 0 0.09 0.07 0 0.05 0 0.07 0 0 0.14 0.13 0.14 0.21 0.18 0.21 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 7 6 3 1 1 3 1 1 3 0 4 14 14 11 7 16 15 6 23 10 22 26 35
Bud 2 4 17 13 20 26 24 18 26 20 27 28 5 1 1 2 8 6 1 2 2 4 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 1 1 2 0 1 4 4 1 5 3 6 4 0 0 0 0 0 1 – – – – – –
Cell Periphery 9 20 12 15 3 4 7 2 8 2 8 8 10 7 14 14 17 20 0 3 0 0 0 1
Cytoplasm 2 6 8 5 9 10 12 9 5 7 5 2 8 10 7 18 9 8 0 0 0 0 0 0
Endoplasmic Reticulum 10 9 4 0 10 1 3 4 6 5 6 3 1 5 5 11 7 4 1 2 0 3 0 2
Endosome 3 2 5 1 13 2 6 6 1 7 2 0 5 2 4 4 5 8 6 12 7 10 7 6
Golgi 24 59 10 43 15 2 1 2 1 6 2 1 100 123 217 67 50 45 38 60 23 37 39 44
Mitochondria 401 535 653 476 633 651 888 810 886 845 868 964 383 413 412 27 17 17 401 574 601 313 264 327
Nucleus 1 1 1 0 2 3 7 4 1 5 2 2 1 1 0 6 2 5 1 2 1 1 1 1
Nuclear Periphery 24 15 47 11 42 69 113 71 90 89 87 92 13 21 16 16 15 25 3 3 2 1 2 2
Nucleolus 37 21 37 19 53 76 114 93 86 73 71 73 12 12 8 19 12 11 3 2 2 3 2 4
Peroxisomes 4 6 22 8 6 3 5 10 2 17 4 4 14 22 18 24 21 22 10 8 25 26 21 36
SpindlePole 0 1 2 3 1 3 4 6 7 8 3 9 1 2 1 9 5 7 0 2 2 0 1 0
Vac/Vac Membrane 59 79 20 13 63 47 32 42 34 61 27 17 65 68 82 49 32 38 14 17 2 21 17 15
Unique Cell Count 460 623 698 503 715 722 983 902 927 924 910 999 468 520 567 232 178 181 492 720 689 451 393 486
Labelled Cell Count 583 766 846 610 872 902 1223 1079 1159 1151 1118 1211 632 701 796 273 216 232 492 720 689 451 393 486


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.7 18.3 10.5 11.2 9.6 9.0 8.3 8.6 8.2 7.9 8.2 7.8 16.0 15.5 15.4 13.0 13.6 12.9 17.8 17.7 18.7
Std Deviation (1e-4) 4.0 5.1 2.4 2.3 2.3 2.0 2.2 2.0 2.1 2.2 2.1 2.0 3.2 3.3 3.1 4.2 4.6 3.7 4.2 4.6 4.4
Intensity Change (Log2) – – – 0.09 -0.13 -0.24 -0.35 -0.29 -0.36 -0.42 -0.36 -0.43 0.6 0.55 0.55 0.31 0.37 0.29 0.76 0.75 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.7 2.5 1.7 0 0 0
Bud 0.2 0.4 1.3 0 -0.6 0.5 -0.4 0.6 0.5 -1.7 -3.2 -3.4 0 1.5 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 -2.4 -2.1 -1.9 -3.2 -1.5 -3.2 -1.5 -1.7 0.5 -0.5 0.9 3.5 5.2 6.0
Cytoplasm -0.3 0.2 0.4 0.1 -0.3 -1.4 -0.8 -1.3 0 0.8 1.1 0.1 5.3 0 0
Endoplasmic Reticulum 0 1.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.8 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Golgi 5.9 0.9 -2.4 0 -2.8 0 -1.6 -2.8 0 11.4 12.3 17.0 13.1 12.6 11.6
Mitochondria 0.8 -3.3 -2.3 -2.4 -2.7 1.8 -1.6 1.6 2.8 -6.2 -7.4 -10.1 -24.4 -23.6 -23.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.6 -0.7 1.9 3.3 0.9 2.1 2.1 2.0 1.8 -3.0 -2.0 -3.2 0.1 0.8 3.1
Nucleolus -1.2 1.6 3.6 4.4 3.6 3.0 2.1 2.0 1.7 -2.3 -2.6 -3.7 1.6 0.7 0.4
Peroxisomes -1.7 -3.1 -3.9 -4.2 -2.9 -4.9 -1.7 -4.3 -4.5 -0.2 1.0 0 4.4 4.8 4.9
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 4.8 3.2 0.5 1.8 0.9 3.4 0.1 -1.6 7.1 6.8 7.5 9.2 7.6 8.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1162 11.6719 10.4798 8.9476 9.4384 10.4952 13.9232 15.4333 15.0012 13.708 14.42 14.9764 12.168 13.6395 12.8857 12.6158 12.2225 12.5929
Actin 0.0057 0.0069 0.0042 0.0031 0.0135 0.0023 0.0115 0.0009 0.0018 0.0001 0.0001 0.0036 0.0037 0.0026 0.0044 0.0006 0.0086 0.0004
Bud 0.0007 0.0009 0.0001 0.0002 0.0005 0.0001 0.0001 0.0011 0.0012 0.0002 0 0 0.0002 0.0003 0.0001 0.0003 0.0007 0
Bud Neck 0.0014 0.0004 0 0.0001 0.0004 0.0001 0.0003 0 0.0001 0 0 0 0.0003 0.0001 0.0001 0 0.0002 0
Bud Periphery 0.001 0.002 0.0004 0.0013 0.0007 0.0002 0.0002 0.0028 0.0034 0.0021 0 0 0.0005 0.0004 0.0001 0.0019 0.0022 0
Bud Site 0.0021 0.0065 0.0001 0.0004 0.0017 0.0001 0.0005 0.0005 0.0007 0 0 0 0.0028 0.0038 0.0002 0 0.0004 0
Cell Periphery 0.0004 0.002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002 0.0005 0.0001 0 0 0.0003 0.0003 0.0001 0.0006 0.0004 0
Cytoplasm 0.0009 0.0002 0.0001 0.0002 0.0013 0.0001 0.0005 0 0.0001 0 0 0 0.0008 0 0.0001 0 0.0007 0
Cytoplasmic Foci 0.0139 0.0027 0.0006 0.0104 0.0264 0.0009 0.0027 0.0006 0.0005 0 0 0.0001 0.0033 0.0009 0.0031 0 0.0023 0.0001
Eisosomes 0.0003 0.0005 0.0002 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0006 0.0002 0.0002 0.0002 0.001 0.0004 0.0006 0 0.0002 0 0 0 0.0008 0.0001 0.0003 0.0001 0.0014 0.0001
Endosome 0.0221 0.0127 0.006 0.0232 0.0152 0.0051 0.006 0.0034 0.0013 0.0001 0.0001 0.0009 0.0064 0.0031 0.0075 0.0005 0.0211 0.0008
Golgi 0.0174 0.0242 0.0162 0.016 0.0187 0.0139 0.0102 0.0285 0.0014 0.0003 0.0006 0.0064 0.0154 0.0119 0.0172 0.0003 0.0428 0.0071
Lipid Particles 0.0281 0.0056 0.0014 0.0202 0.0154 0.0061 0.0179 0.0101 0.001 0.0001 0.0001 0.0012 0.0228 0.0086 0.0048 0.0002 0.0093 0.0011
Mitochondria 0.8449 0.8876 0.958 0.9037 0.8683 0.9581 0.9264 0.9339 0.9826 0.9949 0.9984 0.9849 0.9107 0.9505 0.9453 0.9665 0.855 0.9848
None 0.0009 0.0001 0 0.0002 0.0009 0.0001 0.0007 0 0.0001 0 0 0 0.001 0 0.0002 0 0.0097 0
Nuclear Periphery 0.0035 0.0002 0.0001 0.0012 0.0034 0.0009 0.0042 0 0.0014 0.0001 0 0.0001 0.0106 0.0001 0.0018 0.0001 0.01 0
Nucleolus 0.0032 0.0021 0.0001 0.003 0.0007 0.0001 0.0003 0.0003 0.0001 0 0 0 0.002 0.0012 0.0006 0.0001 0.0019 0
Nucleus 0.0018 0.0008 0.0001 0.0008 0.0007 0.0002 0.0006 0.0001 0.0001 0 0 0 0.0027 0.0002 0.0005 0.0001 0.0021 0
Peroxisomes 0.0198 0.0067 0.0013 0.0049 0.0146 0.0022 0.0098 0.0013 0.0016 0.0002 0.0002 0.0011 0.0089 0.0062 0.0086 0.0004 0.0024 0.0037
Punctate Nuclear 0.0068 0.0001 0 0.0023 0.0022 0.0001 0.0014 0 0.0006 0 0 0 0.0021 0 0.001 0 0.008 0
Vacuole 0.0087 0.0203 0.0037 0.0025 0.0038 0.0012 0.0007 0.0052 0.0006 0.0002 0 0.0002 0.0013 0.0015 0.0006 0.0124 0.0058 0.0001
Vacuole Periphery 0.016 0.0172 0.0069 0.0058 0.0102 0.0076 0.005 0.0108 0.0005 0.0014 0.0003 0.0012 0.0032 0.0079 0.0032 0.0158 0.0145 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.7203 28.5551 35.1134 51.2875 46.1488 33.7497 44.5455 40.9221 44.9017 61.3797
Translational Efficiency 0.7564 0.8239 0.8707 0.6332 0.6347 1.0802 0.5723 0.719 0.7619 0.4738

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘