Standard name
Description Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0.13 0.17 0.18 0.09 0.14 0.07 – 0.15 0.15 0.12 0.1 0.1 0.08 0.14 0.08 0.08 0 0 0 0 0 0.06
Bud 0 0.08 0 0.06 0 0 0.05 0.05 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.15 0.32 0.26 0.41 0.29 0.22 0.18 0.14 – 0.2 0.21 0.22 0.64 0.64 0.66 0.43 0.46 0.43 0 0 0 0.1 0.13 0.13
Cytoplasm 0.24 0.34 0.13 0.14 0.2 0.4 0.4 0.48 – 0.48 0.46 0.43 0.09 0.09 0.08 0.58 0.57 0.52 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.78 0.41 0.71 0.58 0.6 0.53 0.5 0.43 – 0.4 0.36 0.46 0.45 0.45 0.51 0 0.05 0 0.92 0.75 0.87 0.82 0.77 0.73
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 24 47 51 52 28 61 32 – 59 47 51 52 53 44 40 12 15 6 15 15 8 11 18
Bud 8 23 10 17 8 8 24 25 – 15 21 12 5 5 1 3 5 3 2 2 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 2 1 1 0 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 78 95 92 124 85 72 81 65 – 79 67 92 344 344 357 122 71 78 3 9 13 24 41 40
Cytoplasm 125 99 45 43 60 132 177 227 – 187 148 181 48 48 45 167 89 94 15 24 1 1 9 7
Endoplasmic Reticulum 410 122 253 175 176 175 222 205 – 158 114 193 244 246 277 3 7 2 524 298 320 202 256 224
Endosome 0 0 0 0 0 1 0 0 – 0 1 0 0 1 0 1 1 0 0 2 0 0 2 0
Golgi 0 0 9 2 0 0 0 0 – 1 1 0 3 4 3 1 1 6 2 1 1 1 2 1
Mitochondria 0 2 0 0 0 1 2 1 – 3 3 2 0 0 0 4 2 2 0 11 2 2 2 1
Nucleus 1 0 0 0 0 1 1 2 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 4 – 2 1 1 0 0 0 0 0 0 0 3 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 0 0 1 4 – 1 0 1 1 0 2 0 0 3 6 17 3 1 2 4
Unique Cell Count 529 294 357 300 293 328 447 474 391 319 420 540 541 543 287 155 180 567 398 368 245 333 308
Labelled Cell Count 636 365 458 412 381 418 569 566 505 404 534 697 701 729 342 188 204 567 398 368 245 333 308


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.3 28.0 23.1 23.8 21.8 17.6 17.7 17.1 – 16.5 15.8 16.3 23.8 23.8 22.3 17.1 19.6 21.9 26.5 28.3 29.9
Std Deviation (1e-4) 5.0 5.3 4.3 4.3 4.2 3.6 3.5 3.3 – 3.3 3.3 3.1 4.6 4.6 4.4 3.7 4.6 5.2 6.5 6.7 8.1
Intensity Change (Log2) – – – 0.04 -0.08 -0.39 -0.38 -0.44 – -0.49 -0.55 -0.51 0.04 0.04 -0.05 -0.43 -0.24 -0.08 0.2 0.29 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.4 1.6 -1.9 0.2 -3.1 – 0.8 0.6 -0.4 -1.7 -1.6 -2.5 0.3 -1.8 -1.7
Bud 1.8 -0.1 -0.3 1.8 1.8 – 0.8 2.3 0 -2.1 -2.1 0 -1.6 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 4.2 0.9 -1.2 -2.6 -4.4 – -1.8 -1.5 -1.3 11.1 11.1 11.7 4.5 4.5 4.1
Cytoplasm 0.6 2.7 8.3 8.5 10.7 – 10.4 9.7 9.3 -1.8 -1.8 -2.1 12.2 10.6 9.9
Endoplasmic Reticulum -3.4 -2.9 -4.7 -6.1 -7.9 – -8.4 -9.2 -7.0 -7.6 -7.5 -5.9 -18.0 -13.8 -15.3
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi -1.8 0 0 0 0 – 0 0 0 0 -2.2 0 0 0 0.5
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.621 20.2328 18.8911 15.5968 17.3825 19.448 19.891 22.6832 23.6287 19.8322 19.8023 21.5955 16.9916 15.4957 16.5998 17.2505 18.3549 16.8057
Actin 0.0257 0.0015 0.0027 0.021 0.0184 0.0036 0.0391 0.0007 0.0132 0.0006 0.0285 0.0002 0.0506 0.0346 0.0333 0.0329 0.091 0.0528
Bud 0.0005 0.0002 0.0002 0.0007 0.0004 0.0001 0.0026 0 0.0001 0 0.0002 0 0.0255 0.0462 0.0645 0.012 0.0229 0.0069
Bud Neck 0.0012 0.0022 0.0014 0.007 0.0211 0.0107 0.0014 0.0018 0.0025 0.0004 0.0038 0.0009 0.0212 0.0039 0.0062 0.0139 0.0027 0.156
Bud Periphery 0.0009 0.0007 0.0007 0.0027 0.0011 0.0003 0.0054 0.0003 0.0008 0.0001 0.0008 0.0001 0.0593 0.0833 0.124 0.0549 0.0986 0.0361
Bud Site 0.0041 0.0045 0.0008 0.0021 0.0024 0.0005 0.0027 0.0011 0.0004 0 0.0006 0 0.0945 0.1416 0.0379 0.012 0.0025 0.0053
Cell Periphery 0.011 0.0381 0.0282 0.0156 0.0313 0.0049 0.0136 0.0227 0.0172 0.0062 0.0227 0.004 0.0312 0.0419 0.0358 0.0123 0.0198 0.0223
Cytoplasm 0.0492 0.0812 0.0528 0.0398 0.0873 0.0377 0.0174 0.039 0.0289 0.0032 0.0157 0.0141 0.0416 0.0281 0.0197 0.0369 0.0186 0.0332
Cytoplasmic Foci 0.0232 0.0021 0.0023 0.0056 0.0027 0.002 0.014 0.0004 0.0009 0.0002 0.0197 0.0003 0.015 0.0056 0.0048 0.0126 0.0117 0.0098
Eisosomes 0.0004 0.0004 0.0002 0.0008 0.0004 0.0001 0.0005 0.0001 0.0002 0.0006 0.0002 0 0.0031 0.0017 0.001 0.0006 0.003 0.0009
Endoplasmic Reticulum 0.7137 0.8081 0.8491 0.7968 0.7581 0.8947 0.8106 0.9077 0.9119 0.9696 0.8152 0.9712 0.5437 0.4886 0.5632 0.6624 0.6442 0.5958
Endosome 0.0431 0.0015 0.012 0.0117 0.0168 0.0096 0.0052 0.0007 0.0013 0.0032 0.0322 0.0008 0.0041 0.0031 0.0016 0.0054 0.0048 0.0046
Golgi 0.0179 0.0018 0.0063 0.0099 0.0145 0.0035 0.0041 0.0011 0.0035 0.005 0.024 0.0005 0.0051 0.0021 0.0014 0.0029 0.0089 0.0039
Lipid Particles 0.0438 0.0341 0.0195 0.0242 0.0171 0.0049 0.013 0.0136 0.0082 0.0026 0.0148 0.0026 0.0111 0.0185 0.0073 0.0086 0.0086 0.0085
Mitochondria 0.0104 0.0006 0.0013 0.0443 0.0094 0.0018 0.0016 0 0.0001 0.0007 0.0021 0 0.0138 0.017 0.0115 0.0013 0.0034 0.0047
None 0.0058 0.0023 0.003 0.0007 0.0003 0.0011 0.0052 0.0001 0.0001 0.0003 0.0008 0 0.0459 0.0464 0.0581 0.0601 0.0352 0.0358
Nuclear Periphery 0.0181 0.0067 0.0073 0.0063 0.0053 0.0106 0.0207 0.0058 0.0057 0.0047 0.008 0.0027 0.0076 0.013 0.0119 0.0287 0.0092 0.0067
Nucleolus 0.0006 0.0003 0.0002 0.0001 0.0003 0.001 0.0037 0 0 0 0.0001 0 0.0006 0.0012 0.0006 0.0007 0.0002 0.0004
Nucleus 0.0045 0.0016 0.001 0.0008 0.0008 0.0041 0.0085 0.0003 0.0003 0.0001 0.0005 0.0001 0.0016 0.0063 0.0027 0.0036 0.0025 0.0017
Peroxisomes 0.0054 0.0003 0.0001 0.0006 0.0011 0.0002 0.0029 0 0 0 0.0017 0 0.0034 0.0017 0.0002 0.0075 0.0016 0.0026
Punctate Nuclear 0.0045 0.0004 0.0003 0.0003 0.0005 0.0021 0.0218 0.0001 0.0001 0 0.0015 0 0.0017 0.0013 0.0026 0.0016 0.0006 0.0013
Vacuole 0.0139 0.0112 0.0099 0.0075 0.0098 0.0053 0.0053 0.0042 0.0046 0.0021 0.006 0.0023 0.0176 0.0128 0.0102 0.0226 0.0091 0.0095
Vacuole Periphery 0.002 0.0002 0.0008 0.0014 0.001 0.0011 0.0008 0.0002 0.0003 0.0004 0.0007 0.0001 0.0017 0.0011 0.0015 0.0066 0.0008 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 128.2 94.8439 92.2737 112.4493 102.787 108.9833 106.0189 101.9398 102.0127 130.3611
Translational Efficiency 1.4857 1.4846 1.388 1.0827 1.5423 1.7734 1.2274 1.1354 1.1866 1.1984

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘