Standard name
Human Ortholog
Description Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.1 0.1 0.1 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.98 0.92 0.84 0.83 0.73 0.76 0.76 0.98 0.98 0.99 0.99 0.99 1.0 0.98 0.97 0.97 0.93 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 0.14 0.14 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.12 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 8 7 20 18 22 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 2 3 6 4 7 0 0 1 0 1 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 2 4 8 9 20 19 17 58 46 54 4 7 5 24 11 22 0 0 0 0 1 0
Cytoplasm 194 214 322 378 361 460 472 412 444 364 417 296 396 428 393 293 301 190 204 301 221 311 366
Endoplasmic Reticulum 1 1 1 2 6 20 64 70 86 20 42 5 10 9 13 8 10 0 1 2 7 13 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 1 6 13 9 22 56 62 0 0 0 1 0 0 0 1 0 0 4 3
Nucleus 0 0 0 0 0 1 0 3 2 2 0 0 1 0 0 0 0 0 1 1 0 2 1
Nuclear Periphery 0 0 0 0 0 0 4 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 7 6 4 36 26 9 1 1 3 1 0 0 0 0 0 2 5 5
Unique Cell Count 195 216 324 381 367 502 561 499 609 479 546 303 406 434 397 296 302 194 211 311 237 348 403
Labelled Cell Count 198 218 329 388 377 520 590 525 675 539 613 307 415 446 432 313 333 194 211 311 237 348 403


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 58.2 55.8 33.9 31.7 26.1 21.9 19.5 19.4 17.3 16.5 16.3 35.4 36.0 37.4 58.7 59.2 61.2 44.9 46.1 45.1
Std Deviation (1e-4) 8.9 10.6 6.6 5.7 4.9 4.4 4.4 4.5 4.1 4.1 4.0 8.0 7.2 7.0 13.1 13.8 14.4 9.2 11.9 12.2
Intensity Change (Log2) -0.1 -0.37 -0.63 -0.8 -0.81 -0.97 -1.03 -1.06 0.06 0.09 0.14 0.79 0.81 0.85 0.41 0.44 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.3 3.5 3.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 1.2 2.3 1.9 1.9 4.8 4.8 4.9 0 0 0 3.3 2.0 3.8
Cytoplasm -0.3 -1.2 -4.8 -7.2 -7.6 -10.0 -9.2 -9.2 -1.8 -1.9 -1.0 -0.6 -0.6 0.5
Endoplasmic Reticulum 0 0 3.3 6.1 6.8 6.9 3.4 4.9 0 0 0 2.9 0 2.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.3 0 3.1 6.2 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 4.5 4.3 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 41.2731 51.6152 45.1906 43.0472 42.3359 45.6532 42.9803 53.1603 46.187 42.2843 46.8844 47.5873 34.9273 47.0027 41.1432 40.9954 27.9443 42.808
Actin 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0004 0.0002 0.0003 0.0001 0.0002 0.0002 0.002 0.0002 0.0003 0 0.0096 0.0047
Bud 0.0004 0.0001 0 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0002 0.0003 0.0001 0.0007 0.0001 0.0003 0.0001 0.0045 0.0001
Bud Neck 0.0001 0.0001 0.0002 0.0002 0.0004 0.0005 0.0002 0.0001 0.0002 0.0002 0.0002 0.0005 0.0002 0.0001 0.0002 0.0001 0.0011 0.0003
Bud Periphery 0.0003 0 0 0 0 0 0.0002 0 0.0001 0 0.0002 0 0.0004 0 0.0003 0 0.0043 0.0001
Bud Site 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0012 0.0001 0.0001 0 0.0028 0.0001
Cell Periphery 0.0002 0.0001 0 0.0003 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0003 0.0005 0
Cytoplasm 0.9679 0.9949 0.9898 0.9783 0.9895 0.9873 0.9656 0.9933 0.9823 0.9919 0.9767 0.9903 0.8934 0.9841 0.9739 0.9912 0.8254 0.9122
Cytoplasmic Foci 0.0008 0.0004 0.0006 0.0007 0.0012 0.0007 0.0024 0.0005 0.0016 0.001 0.002 0.0023 0.0214 0.0019 0.0009 0.0005 0.0249 0.0042
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0067 0.0023 0.0052 0.002 0.0035 0.0072 0.012 0.0037 0.0063 0.0006 0.0069 0.0028 0.0106 0.0004 0.0054 0.0008 0.002 0.0022
Endosome 0.0012 0.0003 0.0006 0.0012 0.0014 0.0008 0.0045 0.0004 0.0012 0.0012 0.0043 0.0017 0.0179 0.0019 0.0013 0.0008 0.0281 0.0124
Golgi 0.0001 0 0 0 0.0001 0 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0046 0.0003 0.0001 0 0.0187 0.0098
Lipid Particles 0.0001 0 0 0.0001 0 0 0.0002 0 0.0003 0 0 0 0.0059 0.0003 0.0001 0.0002 0.0024 0.0056
Mitochondria 0.0011 0 0.0001 0 0.0001 0 0.0004 0.0001 0.0029 0 0.0008 0.0001 0.0028 0.0014 0.0002 0 0.0492 0.0052
None 0.0007 0.0001 0.0001 0.0002 0.0001 0.0001 0.0019 0.0001 0.001 0.0002 0.0007 0.0002 0.0047 0.0004 0.0043 0.0001 0.0013 0.0002
Nuclear Periphery 0.0048 0.0003 0.0007 0.0011 0.0004 0.001 0.0029 0.0002 0.0009 0.0009 0.0005 0.0004 0.0076 0.0009 0.0015 0.001 0.0008 0.0358
Nucleolus 0.0007 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.001 0.0002 0.0002 0 0.0003 0
Nucleus 0.0058 0.0005 0.0009 0.0013 0.0009 0.001 0.0021 0.0004 0.0007 0.001 0.0011 0.0006 0.0047 0.0058 0.0023 0.0007 0.0015 0.0018
Peroxisomes 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0024 0.0001 0 0 0.0016 0.0004
Punctate Nuclear 0.001 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.002 0.0001 0.0006 0 0.0005 0.0002
Vacuole 0.0059 0.0006 0.0014 0.0133 0.002 0.001 0.0044 0.0005 0.0011 0.0019 0.0045 0.0006 0.0132 0.001 0.0067 0.0034 0.0178 0.0007
Vacuole Periphery 0.0019 0.0001 0.0001 0.0009 0.0003 0.0002 0.0013 0 0.0004 0.0004 0.0011 0.0002 0.0029 0.0006 0.0014 0.0005 0.0028 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.8058 69.8259 80.4876 79.6456 78.6591 78.8251 81.7394 93.9139 82.8307 69.2661
Translational Efficiency 1.5013 1.3539 1.0908 1.2255 1.0729 1.2233 1.3366 1.1819 1.1081 1.0992

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency