Standard name
Description Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.1 0.1 0.1 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.98 0.92 0.84 0.83 0.73 0.76 0.76 0.98 0.98 0.99 0.99 0.99 1.0 0.98 0.97 0.97 0.93 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 0.14 0.14 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.12 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 8 7 20 18 22 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 2 3 6 4 7 0 0 1 0 1 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 2 4 8 9 20 19 17 58 46 54 4 7 5 24 11 22 0 0 0 0 1 0
Cytoplasm 194 214 322 378 361 460 472 412 444 364 417 296 396 428 393 293 301 190 204 301 221 311 366
Endoplasmic Reticulum 1 1 1 2 6 20 64 70 86 20 42 5 10 9 13 8 10 0 1 2 7 13 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 1 6 13 9 22 56 62 0 0 0 1 0 0 0 1 0 0 4 3
Nucleus 0 0 0 0 0 1 0 3 2 2 0 0 1 0 0 0 0 0 1 1 0 2 1
Nuclear Periphery 0 0 0 0 0 0 4 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 7 6 4 36 26 9 1 1 3 1 0 0 0 0 0 2 5 5
Unique Cell Count 195 216 324 381 367 502 561 499 609 479 546 303 406 434 397 296 302 194 211 311 237 348 403
Labelled Cell Count 198 218 329 388 377 520 590 525 675 539 613 307 415 446 432 313 333 194 211 311 237 348 403


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 58.2 55.8 33.9 31.7 26.1 21.9 19.5 19.4 17.3 16.5 16.3 35.4 36.0 37.4 58.7 59.2 61.2 44.9 46.1 45.1
Std Deviation (1e-4) 8.9 10.6 6.6 5.7 4.9 4.4 4.4 4.5 4.1 4.1 4.0 8.0 7.2 7.0 13.1 13.8 14.4 9.2 11.9 12.2
Intensity Change (Log2) -0.1 -0.37 -0.63 -0.8 -0.81 -0.97 -1.03 -1.06 0.06 0.09 0.14 0.79 0.81 0.85 0.41 0.44 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.3 3.5 3.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 1.2 2.3 1.9 1.9 4.8 4.8 4.9 0 0 0 3.3 2.0 3.8
Cytoplasm -0.3 -1.2 -4.8 -7.2 -7.6 -10.0 -9.2 -9.2 -1.8 -1.9 -1.0 -0.6 -0.6 0.5
Endoplasmic Reticulum 0 0 3.3 6.1 6.8 6.9 3.4 4.9 0 0 0 2.9 0 2.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.3 0 3.1 6.2 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 4.5 4.3 0 0 0 0 0 0 0