Standard name
Human Ortholog
Description Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.69 0.64 0.74 0.82 0.92 0.84 0.79 0.81 0.8 0.72 0.77 0.89 0.91 0.93 0.94 0.96 0.97 0.38 0.27 0.4 0.23 0.33 0.23
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.07 0.07 0.05 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.54 0.37 0.46 0.33 0.37
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.41 0.43 0.23 0.14 0 0.06 0.08 0.07 0.06 0.07 0.1 0.18 0.13 0.07 0.1 0.13 0 0.05 0.11 0.17 0.15 0.19 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 5 3 3 4 12 11 0 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 2 2 0 1 0 0 2 1 0 0 0 4 12 7 17 9 13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 2 2 4 4 5 13 8 0 1 0 5 1 1 0 0 0 0 0 0
Cytoplasm 118 184 294 195 315 351 455 353 287 342 245 256 271 346 400 456 262 335 70 92 252 57 80 66
Endoplasmic Reticulum 5 1 3 6 19 16 37 30 17 21 18 10 1 9 12 23 8 15 0 1 4 4 5 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 2 3
Golgi 0 4 13 12 16 1 6 20 5 6 14 6 3 2 1 4 3 6 0 0 0 0 0 0
Mitochondria 0 1 0 1 0 0 0 4 8 20 18 14 1 0 0 0 0 0 0 0 0 0 0 0
Nucleus 5 4 4 5 4 2 15 12 8 14 13 11 3 2 0 1 1 0 94 186 237 116 79 108
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 5 2 5 3
Nucleolus 1 4 1 0 1 1 1 3 7 3 2 2 0 0 0 0 0 0 0 1 1 1 2 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 1 1 3
Vac/Vac Membrane 26 109 198 60 54 15 32 35 26 27 25 34 54 51 32 48 36 13 9 39 106 37 45 60
Unique Cell Count 144 268 456 262 384 382 540 449 354 426 342 333 305 382 432 485 273 346 186 349 637 250 241 291
Labelled Cell Count 155 307 513 280 411 390 554 466 367 442 361 352 333 413 447 537 311 370 186 349 637 250 241 291


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 65.0 50.9 38.0 35.4 36.5 35.6 32.7 31.4 26.9 27.0 24.6 25.3 58.4 58.3 51.0 86.8 92.1 94.4 53.0 58.2 59.6
Std Deviation (1e-4) 12.3 13.6 8.0 7.7 8.1 7.4 7.2 7.6 6.8 6.9 6.4 7.1 15.1 15.1 14.0 30.1 32.7 29.9 15.7 15.7 19.0
Intensity Change (Log2) -0.1 -0.06 -0.1 -0.22 -0.28 -0.5 -0.49 -0.63 -0.59 0.62 0.62 0.42 1.19 1.28 1.31 0.48 0.61 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 4.2 0 0 0 0 0 0 0
Cytoplasm 2.8 5.7 9.4 7.2 4.7 5.2 5.2 2.1 3.7 7.5 8.9 10.1 11.3 9.7 11.0
Endoplasmic Reticulum 0 3.9 3.4 5.0 4.8 3.8 3.9 4.0 2.6 0 2.1 2.5 3.8 0 3.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.2 1.0 -2.9 -2.0 1.3 -1.4 -1.5 1.0 -0.9 -1.8 -2.5 -3.1 -2.3 -1.6 -1.0
Mitochondria 0 0 0 0 0 0 4.7 5.0 4.4 0 0 0 0 0 0
Nucleus 0 0 0 2.2 2.0 1.6 2.5 2.8 2.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.5 -9.2 -13.1 -14.0 -12.3 -11.4 -12.6 -11.2 -10.1 -7.4 -9.5 -12.2 -11.7 -8.5 -12.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 48.5284 63.3357 53.0609 53.4021 58.0337 56.3421 53.3456 68.6355 62.3133 61.3164 50.5274 61.6422 49.7074 61.7944 57.3575 53.3538 50.8469 57.9987
Actin 0.0295 0.0001 0.002 0.0003 0.0001 0.004 0.0103 0.0002 0.0034 0.003 0.001 0.0001 0.0131 0.0001 0 0.0412 0.0004 0.0002
Bud 0.002 0.0011 0.0004 0.0005 0 0.0001 0.0006 0.0001 0.0008 0.001 0.0011 0.0001 0.0017 0.0001 0.0001 0.0002 0.0004 0.0009
Bud Neck 0.0031 0.0001 0.0001 0.0001 0.0003 0.0004 0.0005 0.0001 0.0002 0.0003 0.0006 0.0005 0.0005 0.0001 0.0001 0.0006 0.0002 0.0004
Bud Periphery 0.0018 0.0003 0.0003 0.0004 0 0.0002 0.0005 0 0.0006 0.001 0.0021 0.0001 0.0012 0 0 0.0002 0.0003 0.0004
Bud Site 0.0081 0.0002 0.0003 0.0002 0 0 0.0024 0.0001 0.0019 0.0028 0.0007 0 0.0011 0 0.0001 0.0006 0.0003 0.0001
Cell Periphery 0.0009 0.0001 0.0003 0.0002 0.0001 0.0001 0.0005 0.0001 0.0001 0 0.0019 0.0001 0.0006 0.0001 0.0001 0.0002 0.0006 0.0001
Cytoplasm 0.0838 0.1706 0.0428 0.0855 0.1824 0.1111 0.1307 0.2405 0.1027 0.1352 0.2152 0.1928 0.1014 0.23 0.0887 0.0696 0.1303 0.1425
Cytoplasmic Foci 0.0104 0.0009 0.0147 0.0155 0 0.0007 0.0096 0 0.0037 0.0062 0.0052 0.0011 0.0082 0.0001 0.0064 0.0058 0.0027 0.0002
Eisosomes 0.0005 0 0.0001 0 0 0.0001 0.0002 0 0 0 0.0001 0 0.0001 0 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.0016 0.0001 0.0004 0.0017 0.0001 0.0004 0.0034 0.0001 0.0009 0.0001 0.0046 0.0002 0.0023 0.0001 0.0001 0.0016 0.0009 0.0002
Endosome 0.0043 0 0.0059 0.0019 0 0.0005 0.0071 0 0.0014 0.0032 0.0178 0.0001 0.0053 0.0001 0.0001 0.0038 0.0012 0.0001
Golgi 0.003 0 0.0035 0.0004 0 0.0011 0.0028 0 0.0009 0.005 0.0015 0 0.0009 0 0 0.0025 0.0008 0
Lipid Particles 0.0116 0 0.004 0.0117 0 0.0009 0.0106 0 0.0009 0.0003 0.0042 0.0005 0.003 0 0.001 0.0134 0.0387 0
Mitochondria 0.0088 0.001 0.0126 0.0015 0.0001 0.0019 0.005 0.0005 0.0018 0.003 0.015 0.0007 0.0085 0.0003 0.001 0.0075 0.0259 0.0007
None 0.0019 0.0005 0.002 0.0045 0 0.0008 0.0098 0.0001 0.0026 0.0001 0.0056 0.0009 0.0053 0.0003 0.0022 0.0018 0.0047 0.0003
Nuclear Periphery 0.0288 0.0212 0.023 0.0264 0.0517 0.0333 0.0317 0.0176 0.0153 0.0121 0.0392 0.0263 0.0326 0.0231 0.0213 0.021 0.0359 0.0292
Nucleolus 0.0096 0.001 0.0106 0.0026 0.0013 0.0095 0.0151 0.0024 0.0016 0.0002 0.013 0.0093 0.0161 0.0026 0.0065 0.0025 0.0263 0.0011
Nucleus 0.7645 0.8015 0.8613 0.8348 0.7616 0.8294 0.7388 0.7372 0.8569 0.823 0.6231 0.764 0.7677 0.7406 0.8699 0.8194 0.7185 0.8219
Peroxisomes 0.0024 0.0001 0.0009 0.0005 0 0.0001 0.0041 0 0.0003 0.0029 0.0003 0 0.0007 0 0.0001 0.0008 0.0013 0
Punctate Nuclear 0.0054 0.0002 0.0023 0.007 0.0003 0.0024 0.0064 0.0004 0.0012 0.0003 0.0101 0.0016 0.0145 0.0006 0.0013 0.0014 0.0027 0.0005
Vacuole 0.0095 0.0008 0.0066 0.0023 0.0012 0.0013 0.007 0.0005 0.0017 0.0003 0.0237 0.001 0.0091 0.001 0.0006 0.0019 0.0037 0.0006
Vacuole Periphery 0.0086 0.0004 0.006 0.002 0.0008 0.0017 0.0032 0.0002 0.0008 0.0001 0.0139 0.0007 0.0062 0.0006 0.0006 0.0037 0.0042 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 243.2872 229.0317 258.9736 278.8406 238.668 237.4144 278.1966 294.9717 316.1156 304.8541
Translational Efficiency 1.4728 1.5528 1.4569 1.5567 1.469 1.4568 1.4022 1.2422 1.335 1.3002

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1169 1576 333 1154 1393 884 712 24 2562 2460 1045 1178

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3427.57 3604.52 6975.73 4311.24 3265.48 3601.57 5561.35 5006.72 3339.44 3603.46 6012.06 4325.41
Standard Deviation 757.89 858.96 1323.35 1066.44 620.83 797.85 921.88 1187.01 691.50 837.52 1253.57 1073.53
Intensity Change Log 2 0.072621 1.025158 0.330917 0.141331 0.768140 0.616571 0.106553 0.905466 0.477350

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000104 0.000127 0.000147 0.000477 0.000200 0.000276 0.000169 0.000367 0.000156 0.000180 0.000162 0.000475
Bud Neck 0.001622 0.003490 0.002912 0.012162 0.004694 0.005185 0.003385 0.004036 0.003292 0.004099 0.003234 0.011996
Bud Site 0.000323 0.000505 0.000534 0.006235 0.000720 0.001101 0.000586 0.001301 0.000539 0.000719 0.000569 0.006134
Cell Periphery 0.000193 0.000112 0.000220 0.000432 0.000144 0.000147 0.000177 0.000200 0.000166 0.000125 0.000191 0.000427
Cytoplasm 0.069025 0.031291 0.025866 0.021286 0.044797 0.034168 0.019673 0.004033 0.055852 0.032325 0.021647 0.020935
Cytoplasmic Foci 0.000506 0.000738 0.001851 0.004019 0.000376 0.001074 0.001245 0.002084 0.000436 0.000859 0.001438 0.003980
Eisosomes 0.000004 0.000007 0.000011 0.000026 0.000006 0.000009 0.000014 0.000078 0.000006 0.000008 0.000013 0.000027
Endoplasmic Reticulum 0.000095 0.000180 0.000103 0.001571 0.000350 0.000657 0.000145 0.000227 0.000233 0.000351 0.000131 0.001543
Endosome 0.000008 0.000169 0.000013 0.003798 0.000045 0.000199 0.000010 0.000010 0.000028 0.000180 0.000011 0.003721
Golgi 0.000023 0.000677 0.000048 0.002828 0.000058 0.000153 0.000038 0.000045 0.000042 0.000489 0.000042 0.002771
Lipid Particles 0.000009 0.000011 0.000020 0.001617 0.000033 0.000257 0.000016 0.000012 0.000022 0.000099 0.000017 0.001584
Mitochondria 0.000338 0.000527 0.000936 0.001573 0.000892 0.000970 0.000957 0.001341 0.000639 0.000686 0.000950 0.001568
Mitotic Spindle 0.000063 0.000554 0.000127 0.012730 0.000535 0.002299 0.000107 0.000195 0.000320 0.001181 0.000114 0.012475
None 0.004736 0.004127 0.004234 0.006888 0.003198 0.005783 0.003211 0.001163 0.003900 0.004722 0.003537 0.006771
Nuclear Periphery 0.000067 0.000167 0.000381 0.000685 0.000499 0.000331 0.000329 0.000170 0.000302 0.000226 0.000346 0.000674
Nuclear Periphery Foci 0.000033 0.000025 0.000017 0.003221 0.000167 0.001707 0.000017 0.000005 0.000106 0.000630 0.000017 0.003155
Nucleolus 0.000033 0.000078 0.000090 0.000700 0.000178 0.000582 0.000092 0.000285 0.000112 0.000259 0.000091 0.000691
Nucleus 0.922483* 0.956529* 0.961505* 0.916446* 0.941444* 0.943539* 0.968850* 0.982980* 0.932793* 0.951861* 0.966509* 0.917801*
Peroxisomes 0.000003 0.000011 0.000014 0.000140 0.000010 0.000174 0.000017 0.000141 0.000007 0.000070 0.000016 0.000140
Vacuole 0.000316 0.000435 0.000944 0.002673 0.001351 0.001284 0.000938 0.001291 0.000879 0.000740 0.000940 0.002645
Vacuole Periphery 0.000015 0.000242 0.000028 0.000495 0.000303 0.000107 0.000023 0.000036 0.000172 0.000193 0.000024 0.000485

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.79 -7.19 -9.88 -9.26 -6.45 -0.95 -0.86 -3.38 -2.29 -3.22 -0.63 -4.18 -7.81 -7.23 -6.27
Bud Neck -7.67 -7.55 -13.27 -9.73 -8.58 -0.01 -0.97 -1.43 -1.39 -1.22 -1.97 -4.72 -10.32 -9.05 -8.05
Bud Site -2.91 -5.83 -4.86 -4.45 -3.91 -1.16 -1.76 -1.60 -1.42 -1.42 -1.42 -4.10 -4.50 -4.20 -3.74
Cell Periphery 1.32 -2.28 -1.60 -6.20 1.10 0.57 -3.59 -1.40 -1.45 -0.86 1.71 -3.57 -3.11 -5.92 0.21
Cytoplasm 8.79 11.44 11.53 4.09 -0.32 3.17 11.15 3.88 2.48 0.27 8.82 16.24 12.69 4.97 -1.88
Cytoplasmic Foci -0.55 -6.83 -3.50 -2.40 3.81 -2.25 -6.01 -2.36 -1.68 -0.76 -1.59 -9.03 -3.67 -2.30 2.51
Eisosomes -5.79 -7.62 -20.18 -17.50 -3.25 -2.29 -3.08 -4.00 -3.83 -2.40 -4.07 -4.35 -19.02 -17.14 -1.09
Endoplasmic Reticulum -9.13 -6.17 -3.06 -2.53 -2.68 -0.97 6.46 0.47 1.06 -1.72 -1.08 2.54 -2.24 -1.57 -2.43
Endosome -1.30 -2.05 -2.47 -1.92 -2.44 -2.19 4.28 0.92 2.39 -0.79 -1.79 2.95 -2.40 -1.93 -2.44
Golgi -1.45 -6.26 -2.10 -0.08 -1.88 -1.52 -0.37 -1.12 0.50 -1.04 -1.54 -2.82 -2.02 -0.49 -1.92
Lipid Particles -0.74 -3.16 -2.10 -2.09 -1.89 -1.90 0.05 -0.68 0.80 -0.69 -1.65 -1.34 -1.97 -1.65 -1.87
Mitochondria -8.64 -13.00 -25.01 -20.31 -5.65 -0.24 -5.03 -3.46 -2.73 -2.02 -0.66 -12.40 -16.96 -12.12 -7.58
Mitotic Spindle -0.96 0.65 -4.22 -3.94 -4.44 -2.18 1.69 -0.73 1.59 -1.25 -2.33 1.44 -4.20 -3.22 -4.40
None 0.75 1.43 -4.72 -5.52 -6.88 -1.94 -0.87 0.08 1.25 0.38 -1.00 0.58 -6.84 -5.22 -7.85
Nuclear Periphery -4.67 -8.18 -13.61 -12.15 -3.56 1.93 -1.52 -0.79 -1.04 -0.62 1.81 -7.31 -9.83 -10.68 -4.89
Nuclear Periphery Foci 0.57 0.19 -1.88 -1.92 -1.90 -1.57 1.98 1.99 1.79 0.25 -1.42 1.88 -1.60 -0.36 -1.86
Nucleolus -2.92 -3.57 -8.25 -7.26 -5.82 -2.13 0.40 -1.71 0.40 -1.89 -2.11 -1.67 -6.80 -4.02 -6.34
Nucleus -6.85 -7.75 -1.33 5.07 5.97 -0.36 -7.04 -0.74 -0.61 0.74 -5.49 -10.77 0.25 4.15 7.31
Peroxisomes -1.74 -6.73 -6.23 -4.91 -3.26 -1.20 -4.16 -3.42 0.18 -2.87 -1.30 -7.40 -5.91 -0.29 -3.68
Vacuole -3.04 -8.40 -7.09 -6.79 -3.82 -0.56 -1.63 -1.33 -1.21 -1.03 -0.08 -3.77 -5.76 -5.78 -3.36
Vacuole Periphery -1.61 -6.14 -2.55 0.56 -1.95 0.77 1.10 0.95 1.02 -1.87 -0.15 0.99 0.13 0.40 -2.08