Standard name
Description Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.69 0.64 0.74 0.82 0.92 0.84 0.79 0.81 0.8 0.72 0.77 0.89 0.91 0.93 0.94 0.96 0.97 0.38 0.27 0.4 0.23 0.33 0.23
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.07 0.07 0.05 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.54 0.37 0.46 0.33 0.37
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.41 0.43 0.23 0.14 0 0.06 0.08 0.07 0.06 0.07 0.1 0.18 0.13 0.07 0.1 0.13 0 0.05 0.11 0.17 0.15 0.19 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 5 3 3 4 12 11 0 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 2 2 0 1 0 0 2 1 0 0 0 4 12 7 17 9 13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 2 2 4 4 5 13 8 0 1 0 5 1 1 0 0 0 0 0 0
Cytoplasm 118 184 294 195 315 351 455 353 287 342 245 256 271 346 400 456 262 335 70 92 252 57 80 66
Endoplasmic Reticulum 5 1 3 6 19 16 37 30 17 21 18 10 1 9 12 23 8 15 0 1 4 4 5 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 2 3
Golgi 0 4 13 12 16 1 6 20 5 6 14 6 3 2 1 4 3 6 0 0 0 0 0 0
Mitochondria 0 1 0 1 0 0 0 4 8 20 18 14 1 0 0 0 0 0 0 0 0 0 0 0
Nucleus 5 4 4 5 4 2 15 12 8 14 13 11 3 2 0 1 1 0 94 186 237 116 79 108
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 5 2 5 3
Nucleolus 1 4 1 0 1 1 1 3 7 3 2 2 0 0 0 0 0 0 0 1 1 1 2 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 1 1 3
Vac/Vac Membrane 26 109 198 60 54 15 32 35 26 27 25 34 54 51 32 48 36 13 9 39 106 37 45 60
Unique Cell Count 144 268 456 262 384 382 540 449 354 426 342 333 305 382 432 485 273 346 186 349 637 250 241 291
Labelled Cell Count 155 307 513 280 411 390 554 466 367 442 361 352 333 413 447 537 311 370 186 349 637 250 241 291


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 65.0 50.9 38.0 35.4 36.5 35.6 32.7 31.4 26.9 27.0 24.6 25.3 58.4 58.3 51.0 86.8 92.1 94.4 53.0 58.2 59.6
Std Deviation (1e-4) 12.3 13.6 8.0 7.7 8.1 7.4 7.2 7.6 6.8 6.9 6.4 7.1 15.1 15.1 14.0 30.1 32.7 29.9 15.7 15.7 19.0
Intensity Change (Log2) -0.1 -0.06 -0.1 -0.22 -0.28 -0.5 -0.49 -0.63 -0.59 0.62 0.62 0.42 1.19 1.28 1.31 0.48 0.61 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 4.2 0 0 0 0 0 0 0
Cytoplasm 2.8 5.7 9.4 7.2 4.7 5.2 5.2 2.1 3.7 7.5 8.9 10.1 11.3 9.7 11.0
Endoplasmic Reticulum 0 3.9 3.4 5.0 4.8 3.8 3.9 4.0 2.6 0 2.1 2.5 3.8 0 3.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.2 1.0 -2.9 -2.0 1.3 -1.4 -1.5 1.0 -0.9 -1.8 -2.5 -3.1 -2.3 -1.6 -1.0
Mitochondria 0 0 0 0 0 0 4.7 5.0 4.4 0 0 0 0 0 0
Nucleus 0 0 0 2.2 2.0 1.6 2.5 2.8 2.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.5 -9.2 -13.1 -14.0 -12.3 -11.4 -12.6 -11.2 -10.1 -7.4 -9.5 -12.2 -11.7 -8.5 -12.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uba1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available