Standard name
Description Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.23 0.05 0 0.05 0.05 0.05 0 0 0 0 – 0 0.12 0.15 0.11 0.12 0 0 0.07 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0 0 0 0.06 0 0 0.16 0.12 0.25 0.15 – 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.93 0.93 0.73 0.92 0.95 0.88 0.88 0.88 0.8 0.85 0.8 0.82 – 0.96 0.88 0.74 0.75 0.73 0.86 0.87 0.73 0.84 0.71 0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.26 0.05 0 0 0 0.05 0.05 0.05 0.18 0.14 0.27 0.17 – 0 0 0.05 0.09 0.09 0.09 0.06 0 0.09 0.16 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.09 0 0 0 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 1 2 1 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 1 1 1 2 4 4 1 – 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 11 6 8 9 12 19 19 10 12 3 13 – 1 16 20 21 27 0 1 3 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 1 0 0 – 0 0 1 6 4 0 0 0 0 0 0
Endosome 0 0 0 2 1 2 1 1 0 0 0 0 – 0 0 3 4 3 0 1 0 0 0 1
Golgi 0 0 0 0 1 1 0 0 0 0 0 0 – 0 0 1 2 1 0 0 0 0 0 0
Mitochondria 23 3 0 3 2 16 17 17 48 44 61 53 – 0 1 8 7 6 0 1 0 0 0 0
Nucleus 111 196 19 143 300 220 336 336 245 314 197 296 – 24 118 97 143 160 100 195 36 35 69 127
Nuclear Periphery 0 0 0 0 0 0 3 1 1 3 4 2 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 31 11 0 3 6 13 20 20 55 50 65 62 – 0 3 7 17 19 10 13 1 3 15 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 1 4 7 2 5 5 8 2 5 6 – 0 4 5 3 3 0 0 0 0 1 0
Vac/Vac Membrane 1 2 1 1 4 3 6 4 0 1 1 4 – 0 1 2 7 19 4 8 1 0 6 4
Unique Cell Count 120 210 26 155 317 249 380 380 306 368 245 360 25 134 131 190 218 116 225 50 42 99 159
Labelled Cell Count 168 225 27 164 330 270 408 404 371 433 341 437 25 144 144 210 243 116 225 50 42 99 159


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.9 8.4 6.7 7.3 6.1 6.5 6.5 5.3 5.6 5.3 5.6 – 6.9 6.9 9.4 9.4 8.6 – 5.6 7.3
Std Deviation (1e-4) 0.8 1.1 2.4 1.6 1.6 1.6 1.7 1.7 1.1 1.3 1.2 1.3 – 1.4 1.7 2.4 2.6 2.1 – 0.7 1.7
Intensity Change (Log2) – – – -0.33 -0.2 -0.46 -0.37 -0.37 -0.66 -0.58 -0.66 -0.59 – -0.29 -0.29 0.15 0.15 0.02 – -0.6 -0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.9 0 – 0 0 0 0 0
Nucleus 3.0 4.1 2.2 2.3 2.3 0.8 1.7 0.9 1.2 – 2.3 2.0 0.1 0.2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3.0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6777 1.2517 1.2896 0.9155 0.9462 1.1453 2.2387 2.2177 1.9239 1.7338 1.531 2.0905 1.2267 1.0995 0.56 0.9209 0.5993 0.8904
Actin 0.0317 0 0.0094 0.038 0.01 0.0004 0.0116 0 0.0203 0 0.0129 0.0145 0.0381 0 0.0174 0.0316 0 0.0054
Bud 0.0003 0 0.0003 0.0016 0.0006 0.0001 0.0011 0 0.001 0 0.0003 0.0002 0.0006 0.0001 0.0001 0.0001 0 0.0002
Bud Neck 0.0021 0.0001 0.0004 0.002 0.0002 0.0011 0.0012 0.0001 0.0005 0.0004 0.0007 0.0012 0.0026 0.0001 0.0005 0.0008 0.0003 0.001
Bud Periphery 0.0009 0 0.0007 0.0027 0.0007 0.0003 0.0021 0 0.0017 0 0.0007 0.0003 0.0013 0.0001 0.0002 0.0001 0 0.0003
Bud Site 0.0013 0.0001 0.0007 0.0118 0.0006 0.0002 0.0057 0 0.0034 0 0.0008 0.0003 0.0026 0.0002 0.0015 0.0003 0.0001 0.0003
Cell Periphery 0.0002 0 0.0001 0.0006 0.0001 0.0001 0.0005 0 0.0003 0 0.0001 0.0001 0.0003 0 0.0001 0 0 0
Cytoplasm 0.0068 0.0013 0.0002 0.0006 0.0024 0.0004 0.005 0 0.0036 0.0001 0.0002 0.0003 0.0032 0.0005 0.0008 0.0003 0.0001 0.0007
Cytoplasmic Foci 0.0215 0.0001 0.0014 0.0158 0.0063 0.0009 0.0354 0 0.0088 0 0.0098 0.004 0.0207 0.0003 0.0088 0.0129 0 0.0015
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0002 0.0001 0.0003 0 0.0003 0.0004 0 0
Endoplasmic Reticulum 0.0069 0.0001 0.0003 0.0003 0.0039 0.0006 0.0026 0 0.0013 0 0.0001 0.0003 0.0029 0 0.0003 0.0002 0 0.0013
Endosome 0.0234 0.0002 0.0025 0.0211 0.0454 0.0055 0.0307 0 0.0069 0 0.0033 0.003 0.0219 0 0.0031 0.0021 0 0.0089
Golgi 0.0087 0 0.002 0.0219 0.0165 0.0013 0.0092 0 0.0033 0 0.0052 0.0047 0.0056 0 0.0024 0.0031 0 0.0016
Lipid Particles 0.033 0 0.0015 0.0121 0.0026 0.0021 0.0281 0 0.0023 0 0.009 0.011 0.0123 0.0001 0.0033 0.0037 0 0.001
Mitochondria 0.0182 0.0001 0.0117 0.0394 0.043 0.0027 0.0144 0.0001 0.0138 0.0002 0.0656 0.0026 0.0076 0.0002 0.0022 0.0005 0.0002 0.0017
None 0.0207 0.0006 0.0002 0.0002 0.0008 0.0023 0.0208 0 0.0004 0 0.0004 0.0004 0.0058 0.0001 0.0018 0.0012 0 0.0005
Nuclear Periphery 0.0118 0.0014 0.0044 0.0007 0.0031 0.0023 0.0062 0.0003 0.0056 0.0011 0.001 0.0139 0.0077 0.0018 0.0063 0.0005 0.0009 0.0052
Nucleolus 0.0522 0.0238 0.0389 0.0201 0.057 0.1504 0.0337 0.0267 0.0404 0.0738 0.2156 0.0955 0.0406 0.039 0.0287 0.0347 0.0793 0.0853
Nucleus 0.7335 0.9688 0.9153 0.7684 0.7991 0.8197 0.7492 0.9722 0.8662 0.922 0.6372 0.8344 0.8019 0.9467 0.9104 0.8924 0.9166 0.8797
Peroxisomes 0.0125 0 0.0039 0.0253 0.0034 0.0002 0.0213 0 0.009 0 0.0303 0.0073 0.0059 0.0001 0.006 0.0126 0 0.0004
Punctate Nuclear 0.0095 0.0032 0.0007 0.0016 0.0019 0.0085 0.0032 0.0005 0.0011 0.0022 0.0059 0.0045 0.0116 0.0105 0.0053 0.0024 0.0022 0.0041
Vacuole 0.0023 0.0002 0.0032 0.0087 0.0009 0.0005 0.0135 0 0.0073 0.0001 0.0003 0.0005 0.0045 0.0001 0.0002 0.0001 0.0001 0.0004
Vacuole Periphery 0.0021 0 0.002 0.0069 0.0015 0.0005 0.0043 0 0.0027 0.0001 0.0006 0.0009 0.0017 0 0.0003 0.0001 0 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1361 8.4306 12.9027 14.5361 9.1706 3.5346 13.4522 16.3607 14.828 16.1071
Translational Efficiency 1.6622 2.2409 1.4133 1.1634 1.7508 4.0016 1.2918 1.2834 1.3086 1.1789

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘