Standard name
Description Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 – 0.06 0.09 0 0 0 0 0 0 0 0 0.05 0 0 0.12 0.12 0.18 0.13 0.19 0.07 0.15 0.19 0.19
Bud 0.09 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.08 0.06 0.06 0.18 0.14 0.14
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.15 0.24 0.39 0.3 0.14
Bud Site 0.07 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.1 – 0.06 0.09 0.05 0 0 0 0 0 0 0 0.11 0.2 0.16 0.18 0.3 0.3 0.12 0.05 0.07 0 0 0
Cytoplasm 0.74 – 0.94 0.79 0.83 0.81 0.9 0.9 0.87 0.94 0.84 0.93 0.84 0.98 0.99 0.76 0.62 0.62 0.36 0.41 0.43 0.14 0.27 0.43
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 – 0 0.08 0.11 0.16 0.05 0 0.05 0 0 0 0.11 0 0 0 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 29 – 1 27 16 13 6 9 2 5 1 1 2 3 4 28 39 57 28 6 3 25 46 53
Bud 16 – 0 7 5 5 11 15 11 6 6 3 1 4 2 9 26 28 16 2 3 31 33 39
Bud Neck 1 – 0 9 17 11 9 18 11 2 1 1 0 3 1 0 9 1 33 5 12 65 73 41
Bud Site 13 – 0 3 9 3 1 0 0 3 1 1 1 1 0 3 5 7 – – – – – –
Cell Periphery 19 – 1 25 23 4 16 12 8 3 3 8 4 57 55 43 100 94 26 1 3 6 7 8
Cytoplasm 135 – 16 230 422 287 548 556 451 374 165 249 31 284 334 178 209 193 75 13 22 24 66 124
Endoplasmic Reticulum 3 – 0 4 0 0 0 2 0 1 0 0 0 1 3 13 14 10 8 0 0 2 4 2
Endosome 2 – 0 1 1 5 4 1 9 1 1 2 0 0 0 3 6 4 4 0 0 3 1 3
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0
Mitochondria 6 – 0 0 1 1 5 8 9 5 4 2 0 0 0 2 4 1 5 0 2 2 1 2
Nucleus 0 – 0 3 5 4 7 3 3 2 0 2 0 1 0 1 7 0 0 0 0 0 0 1
Nuclear Periphery 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 1 2 1 2 3 9 1 1 2 0 0 0 1 4 0 0 0 0 0 0 0
Peroxisomes 6 – 0 2 2 2 1 0 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 9 – 0 22 55 56 29 23 26 11 7 6 4 2 0 10 27 14 0 0 2 2 2 1
Vac/Vac Membrane 0 – 0 6 6 3 24 5 22 5 15 14 0 0 0 6 24 15 9 0 0 2 1 1
Unique Cell Count 182 17 290 506 355 609 617 518 400 196 269 37 290 339 234 337 312 209 33 53 170 245 286
Labelled Cell Count 239 18 341 565 395 663 655 562 419 206 291 43 356 399 297 477 427 209 33 53 170 245 286


Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 – 5.9 7.6 6.9 6.2 6.0 5.8 5.6 5.5 6.9 6.0 7.3 7.1 6.8 9.4 10.3 11.2 6.6 7.2 7.4
Std Deviation (1e-4) 1.0 – 0.6 7.2 1.8 7.4 5.5 1.2 1.2 1.0 4.2 1.2 1.2 1.5 1.2 1.8 2.0 2.0 1.3 1.2 1.5
Intensity Change (Log2) – – – 0.38 0.24 0.09 0.03 -0.02 -0.06 -0.08 0.23 0.04 0.32 0.28 0.22 0.69 0.82 0.94 0.17 0.29 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -1.2 -1.4 -0.6 -0.5 -0.9 -0.1 -1.1 -0.2 -1.0 1.0 1.4 -1.7 -2.7 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mob2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available