Standard name | |
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Description | Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 29 | – | 1 | 27 | 16 | 13 | 6 | 9 | 2 | 5 | 1 | 1 | 2 | 3 | 4 | 28 | 39 | 57 | 28 | 6 | 3 | 25 | 46 | 53 |
Bud | 16 | – | 0 | 7 | 5 | 5 | 11 | 15 | 11 | 6 | 6 | 3 | 1 | 4 | 2 | 9 | 26 | 28 | 16 | 2 | 3 | 31 | 33 | 39 |
Bud Neck | 1 | – | 0 | 9 | 17 | 11 | 9 | 18 | 11 | 2 | 1 | 1 | 0 | 3 | 1 | 0 | 9 | 1 | 33 | 5 | 12 | 65 | 73 | 41 |
Bud Site | 13 | – | 0 | 3 | 9 | 3 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 3 | 5 | 7 | – | – | – | – | – | – |
Cell Periphery | 19 | – | 1 | 25 | 23 | 4 | 16 | 12 | 8 | 3 | 3 | 8 | 4 | 57 | 55 | 43 | 100 | 94 | 26 | 1 | 3 | 6 | 7 | 8 |
Cytoplasm | 135 | – | 16 | 230 | 422 | 287 | 548 | 556 | 451 | 374 | 165 | 249 | 31 | 284 | 334 | 178 | 209 | 193 | 75 | 13 | 22 | 24 | 66 | 124 |
Endoplasmic Reticulum | 3 | – | 0 | 4 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 13 | 14 | 10 | 8 | 0 | 0 | 2 | 4 | 2 |
Endosome | 2 | – | 0 | 1 | 1 | 5 | 4 | 1 | 9 | 1 | 1 | 2 | 0 | 0 | 0 | 3 | 6 | 4 | 4 | 0 | 0 | 3 | 1 | 3 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | – | 0 | 0 | 1 | 1 | 5 | 8 | 9 | 5 | 4 | 2 | 0 | 0 | 0 | 2 | 4 | 1 | 5 | 0 | 2 | 2 | 1 | 2 |
Nucleus | 0 | – | 0 | 3 | 5 | 4 | 7 | 3 | 3 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 1 | 2 | 1 | 2 | 3 | 9 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 6 | – | 0 | 2 | 2 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 9 | – | 0 | 22 | 55 | 56 | 29 | 23 | 26 | 11 | 7 | 6 | 4 | 2 | 0 | 10 | 27 | 14 | 0 | 0 | 2 | 2 | 2 | 1 |
Vac/Vac Membrane | 0 | – | 0 | 6 | 6 | 3 | 24 | 5 | 22 | 5 | 15 | 14 | 0 | 0 | 0 | 6 | 24 | 15 | 9 | 0 | 0 | 2 | 1 | 1 |
Unique Cell Count | 182 | 17 | 290 | 506 | 355 | 609 | 617 | 518 | 400 | 196 | 269 | 37 | 290 | 339 | 234 | 337 | 312 | 209 | 33 | 53 | 170 | 245 | 286 | |
Labelled Cell Count | 239 | 18 | 341 | 565 | 395 | 663 | 655 | 562 | 419 | 206 | 291 | 43 | 356 | 399 | 297 | 477 | 427 | 209 | 33 | 53 | 170 | 245 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 5.8 | – | 5.9 | 7.6 | 6.9 | 6.2 | 6.0 | 5.8 | 5.6 | 5.5 | 6.9 | 6.0 | 7.3 | 7.1 | 6.8 | 9.4 | 10.3 | 11.2 | 6.6 | 7.2 | 7.4 |
Std Deviation (1e-4) | 1.0 | – | 0.6 | 7.2 | 1.8 | 7.4 | 5.5 | 1.2 | 1.2 | 1.0 | 4.2 | 1.2 | 1.2 | 1.5 | 1.2 | 1.8 | 2.0 | 2.0 | 1.3 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.38 | 0.24 | 0.09 | 0.03 | -0.02 | -0.06 | -0.08 | 0.23 | 0.04 | 0.32 | 0.28 | 0.22 | 0.69 | 0.82 | 0.94 | 0.17 | 0.29 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.5 | -1.2 | -1.4 | -0.6 | -0.5 | -0.9 | -0.1 | -1.1 | -0.2 | -1.0 | 1.0 | 1.4 | -1.7 | -2.7 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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