Standard name
Description Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; two functional forms of Hac1p are produced; translation initiation is repressed under non-stress conditions; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.18 0.35 0.72 0.72 0.66 0.68 0.59 0.6 0.51 0.53 0.17 0.32 0.43 0.35 0.27 0.33 0.11 0.13 0.22 0.17 0.12 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.1 0 0 0 0 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.4 0.18 0.43 0.35 0.45 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.51 0.39 0.46 0.17 0.13 0.12 0.1 0.11 0.15 0.14 0.15 0.64 0.36 0.19 0.24 0.26 0.08 0.64 0.59 0.44 0.47 0.33 0.33
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.16
Vac/Vac Membrane 0.42 0.59 0.23 0.14 0.17 0 0.13 0.07 0 0 0 0.25 0.4 0.48 0.49 0.58 0.62 0.09 0.17 0.19 0.14 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud 0 1 0 0 1 4 1 3 3 4 4 0 0 0 0 0 0 8 7 3 9 21 10
Bud Neck 0 0 2 1 0 0 6 1 2 3 0 0 0 0 0 0 0 1 2 0 2 4 3
Bud Site 0 0 0 0 0 2 0 2 3 2 5 0 0 0 0 0 0
Cell Periphery 0 0 2 0 0 0 2 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 41 44 74 146 175 154 218 227 223 159 198 47 104 168 80 52 64 25 34 60 39 43 31
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 0 10 5 6 2 2 0 1 2 2
Endosome 3 5 3 2 3 0 2 2 1 1 0 6 14 16 12 10 19 5 2 2 7 28 20
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 1 3 2
Mitochondria 7 7 3 9 9 95 58 166 132 141 201 1 2 0 1 2 6 1 2 10 6 7 5
Nucleus 106 97 99 34 32 28 31 44 54 44 55 179 114 72 55 50 16 154 162 121 111 118 98
Nuclear Periphery 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 2 1 0 2 1 4 4 1 6 6 14 11
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 3 1 1 2 3 5 2 2 3 0 1 1 3 2 6 6 7 9 14 42 46
Vac/Vac Membrane 87 146 48 29 42 9 41 27 14 11 13 70 127 187 112 112 121 22 45 53 34 62 51
Unique Cell Count 209 246 213 202 243 235 321 388 372 310 376 279 321 387 228 193 195 240 273 275 238 361 297
Labelled Cell Count 245 300 236 223 263 295 364 479 436 368 479 306 363 444 277 235 242 240 273 275 238 361 297


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 5.8 5.3 5.6 3.8 4.6 3.8 3.9 4.0 3.8 7.3 7.1 6.9 7.8 8.0 7.4 5.8 6.0 6.5
Std Deviation (1e-4) 1.1 1.1 1.1 1.2 1.5 1.2 1.4 1.2 1.0 1.5 1.1 1.6 1.6 1.8 2.2 2.1 1.6 1.6 1.7 2.0
Intensity Change (Log2) -0.12 -0.05 -0.59 -0.34 -0.59 -0.56 -0.55 -0.62 0.33 0.3 0.25 0.42 0.47 0.35 -0.0 0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.7 8.0 6.5 7.5 5.6 5.9 3.7 4.2 -4.6 -0.6 2.1 0.1 -1.7 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.9 1.8 2.2 2.2 3.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 1.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -6.5 -7.8 -8.1 -9.7 -9.7 -8.5 -8.1 -8.5 3.9 -2.5 -7.2 -4.9 -4.3 -8.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 -1.4 -5.9 -3.0 -5.5 -7.1 -6.7 -7.3 0.7 4.1 6.2 5.8 7.3 8.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hac1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available