Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; two functional forms of Hac1p are produced; translation initiation is repressed under non-stress conditions; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.18 0.35 0.72 0.72 0.66 0.68 0.59 0.6 0.51 0.53 0.17 0.32 0.43 0.35 0.27 0.33 0.11 0.13 0.22 0.17 0.12 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.1 0 0 0 0 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.4 0.18 0.43 0.35 0.45 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.51 0.39 0.46 0.17 0.13 0.12 0.1 0.11 0.15 0.14 0.15 0.64 0.36 0.19 0.24 0.26 0.08 0.64 0.59 0.44 0.47 0.33 0.33
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.16
Vac/Vac Membrane 0.42 0.59 0.23 0.14 0.17 0 0.13 0.07 0 0 0 0.25 0.4 0.48 0.49 0.58 0.62 0.09 0.17 0.19 0.14 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud 0 1 0 0 1 4 1 3 3 4 4 0 0 0 0 0 0 8 7 3 9 21 10
Bud Neck 0 0 2 1 0 0 6 1 2 3 0 0 0 0 0 0 0 1 2 0 2 4 3
Bud Site 0 0 0 0 0 2 0 2 3 2 5 0 0 0 0 0 0
Cell Periphery 0 0 2 0 0 0 2 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 41 44 74 146 175 154 218 227 223 159 198 47 104 168 80 52 64 25 34 60 39 43 31
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 0 10 5 6 2 2 0 1 2 2
Endosome 3 5 3 2 3 0 2 2 1 1 0 6 14 16 12 10 19 5 2 2 7 28 20
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 1 3 2
Mitochondria 7 7 3 9 9 95 58 166 132 141 201 1 2 0 1 2 6 1 2 10 6 7 5
Nucleus 106 97 99 34 32 28 31 44 54 44 55 179 114 72 55 50 16 154 162 121 111 118 98
Nuclear Periphery 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 2 1 0 2 1 4 4 1 6 6 14 11
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 3 1 1 2 3 5 2 2 3 0 1 1 3 2 6 6 7 9 14 42 46
Vac/Vac Membrane 87 146 48 29 42 9 41 27 14 11 13 70 127 187 112 112 121 22 45 53 34 62 51
Unique Cell Count 209 246 213 202 243 235 321 388 372 310 376 279 321 387 228 193 195 240 273 275 238 361 297
Labelled Cell Count 245 300 236 223 263 295 364 479 436 368 479 306 363 444 277 235 242 240 273 275 238 361 297


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 5.8 5.3 5.6 3.8 4.6 3.8 3.9 4.0 3.8 7.3 7.1 6.9 7.8 8.0 7.4 5.8 6.0 6.5
Std Deviation (1e-4) 1.1 1.1 1.1 1.2 1.5 1.2 1.4 1.2 1.0 1.5 1.1 1.6 1.6 1.8 2.2 2.1 1.6 1.6 1.7 2.0
Intensity Change (Log2) -0.12 -0.05 -0.59 -0.34 -0.59 -0.56 -0.55 -0.62 0.33 0.3 0.25 0.42 0.47 0.35 -0.0 0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.7 8.0 6.5 7.5 5.6 5.9 3.7 4.2 -4.6 -0.6 2.1 0.1 -1.7 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.9 1.8 2.2 2.2 3.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 1.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -6.5 -7.8 -8.1 -9.7 -9.7 -8.5 -8.1 -8.5 3.9 -2.5 -7.2 -4.9 -4.3 -8.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 -1.4 -5.9 -3.0 -5.5 -7.1 -6.7 -7.3 0.7 4.1 6.2 5.8 7.3 8.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2219 3.0638 2.1479 1.6295 2.2223 2.093 4.2809 4.0348 3.7433 3.1076 3.4226 3.6803 6.5515 7.3769 6.1805 6.2785 6.0001 6.7201
Actin 0.01 0.0001 0.0229 0.0001 0.0007 0.0001 0.0364 0.0004 0.0063 0.0243 0.043 0.0001 0.0007 0.0001 0.0007 0.0002 0.0002 0.0005
Bud 0.0002 0.0006 0.0003 0 0.0002 0.0001 0.0006 0.0003 0.0003 0.0002 0.0003 0.0002 0.0003 0.0001 0.0001 0.0002 0 0.0001
Bud Neck 0.0008 0.0005 0.0008 0.0005 0.0013 0.0034 0.018 0.0006 0.0009 0.0007 0.0011 0.0025 0.0003 0.0003 0.0003 0.0006 0.0004 0.0026
Bud Periphery 0.0002 0.0004 0.0002 0 0.0001 0 0.0008 0.0002 0.0005 0.0002 0.0003 0.0001 0.001 0 0.0001 0.0001 0 0.0001
Bud Site 0.0006 0.0007 0.0009 0.0001 0.0004 0.0001 0.0044 0.0041 0.0005 0.0008 0.0003 0.0003 0.0002 0.0004 0.0003 0.0022 0.0001 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0003 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.083 0.1684 0.1747 0.0908 0.5238 0.0441 0.0565 0.1039 0.0869 0.0224 0.0931 0.0461 0.0218 0.0692 0.0864 0.0455 0.0589 0.0332
Cytoplasmic Foci 0.0207 0.0004 0.006 0.0003 0.0103 0.0003 0.0139 0.0012 0.0033 0.013 0.0046 0.0002 0.0001 0.0002 0.0008 0.0008 0.0025 0.0008
Eisosomes 0.0004 0 0.0001 0 0 0 0.0002 0 0.0001 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0085 0.003 0.0041 0.0005 0.0068 0.0008 0.0081 0.0037 0.002 0.0012 0.0039 0.0012 0.0007 0.0007 0.0019 0.0005 0.0038 0.0016
Endosome 0.039 0.0012 0.0089 0.0004 0.0091 0.0007 0.0297 0.0033 0.005 0.0084 0.0064 0.0006 0.0006 0.0005 0.0014 0.0007 0.0087 0.0022
Golgi 0.0071 0 0.0043 0 0.0003 0 0.0079 0.0003 0.0022 0.0083 0.0048 0 0 0 0.0004 0.0001 0.0001 0.0001
Lipid Particles 0.0096 0 0.0027 0 0.0001 0 0.0081 0.0002 0.0011 0.0147 0.0601 0 0.0001 0 0.0002 0.0001 0.0006 0.0001
Mitochondria 0.0039 0.0003 0.0011 0.0002 0.0005 0.0002 0.003 0.0006 0.0078 0.002 0.0033 0.0003 0.0098 0.0002 0.0068 0.0002 0.0003 0.0004
None 0.029 0.0022 0.0055 0.0011 0.0075 0.001 0.0052 0.0039 0.0024 0.0014 0.0009 0.0006 0.013 0.0009 0.0136 0.0189 0.0164 0.0031
Nuclear Periphery 0.0441 0.0169 0.024 0.0074 0.0169 0.0105 0.0203 0.0322 0.0189 0.0102 0.0248 0.0082 0.0068 0.0073 0.0108 0.0077 0.0104 0.0114
Nucleolus 0.0021 0.0018 0.0015 0.0006 0.0005 0.0024 0.0029 0.0014 0.0012 0.0009 0.001 0.0019 0.0027 0.002 0.0009 0.0017 0.0009 0.0024
Nucleus 0.7099 0.7955 0.7228 0.8943 0.4072 0.9298 0.7631 0.8358 0.8499 0.8816 0.6895 0.933 0.9377 0.9152 0.8702 0.9085 0.8911 0.9303
Peroxisomes 0.0039 0 0.0054 0 0.0002 0 0.0045 0.0001 0.0025 0.0021 0.0546 0 0.0001 0 0.0005 0.0001 0.0001 0
Punctate Nuclear 0.023 0.0046 0.0115 0.0032 0.0127 0.0051 0.0113 0.0055 0.0066 0.0063 0.0061 0.0034 0.0026 0.002 0.004 0.0112 0.0035 0.0101
Vacuole 0.0026 0.0026 0.0017 0.0004 0.001 0.0012 0.0035 0.0021 0.0013 0.0007 0.0013 0.0012 0.0008 0.0008 0.0005 0.0004 0.0015 0.0009
Vacuole Periphery 0.0013 0.0004 0.0005 0.0001 0.0003 0.0002 0.0012 0.0004 0.0004 0.0003 0.0005 0.0002 0.0007 0.0001 0.0002 0.0001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1121.8715 962.9893 1030.0155 831.1393 802.1005 1408.8688 1218.4878 1086.672 1202.34 968.0537
Translational Efficiency 0.0098 0.0111 0.0114 0.0135 0.0211 0.0166 0.0124 0.0191 0.0139 0.0204

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1275 431 201 1905 2254 2134 390 1490 3529 2565 591 3395

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 776.50 862.99 1063.30 1162.97 735.87 863.72 1145.07 1321.63 750.55 863.60 1117.26 1232.60
Standard Deviation 92.13 106.80 135.57 178.78 87.12 112.27 193.73 193.91 91.08 111.37 180.32 201.59
Intensity Change Log 2 0.152358 0.453491 0.582756 0.231113 0.637913 0.844796 0.191215 0.546171 0.716202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000027 0.000684 0.001609 0.001113 0.000028 0.000204 0.000961 0.001213 0.000028 0.000285 0.001181 0.001157
Bud Neck 0.003947 0.006235 0.004404 0.016907 0.002284 0.006552 0.004292 0.023818 0.002885 0.006499 0.004330 0.019940
Bud Site 0.001666 0.003081 0.004848 0.006036 0.000629 0.004254 0.002029 0.014680 0.001003 0.004057 0.002988 0.009830
Cell Periphery 0.000057 0.000127 0.000175 0.000163 0.000057 0.000156 0.000171 0.000146 0.000057 0.000151 0.000172 0.000155
Cytoplasm 0.235191* 0.145807* 0.055376 0.067311 0.287949* 0.148942* 0.062653 0.092177 0.268888* 0.148415* 0.060178 0.078224
Cytoplasmic Foci 0.015755 0.021053 0.001689 0.002370 0.019741 0.008083 0.000412 0.004409 0.018301 0.010262 0.000846 0.003265
Eisosomes 0.000014 0.000036 0.000056 0.000022 0.000015 0.000016 0.000036 0.000017 0.000014 0.000019 0.000043 0.000020
Endoplasmic Reticulum 0.000723 0.006514 0.017278 0.004049 0.000691 0.002123 0.010009 0.002248 0.000702 0.002860 0.012481 0.003259
Endosome 0.000444 0.001370 0.001418 0.006814 0.000668 0.001275 0.000808 0.004680 0.000587 0.001291 0.001016 0.005878
Golgi 0.000171 0.000566 0.000121 0.002513 0.000286 0.000585 0.000079 0.007170 0.000244 0.000582 0.000093 0.004557
Lipid Particles 0.001134 0.002281 0.001174 0.000319 0.000881 0.000739 0.000703 0.000308 0.000973 0.000998 0.000863 0.000314
Mitochondria 0.003021 0.001668 0.001084 0.005318 0.001513 0.002440 0.000900 0.004615 0.002058 0.002310 0.000963 0.005009
Mitotic Spindle 0.002027 0.000306 0.002806 0.046556 0.001507 0.004513 0.001153 0.090167 0.001695 0.003806 0.001715 0.065696
None 0.003385 0.004610 0.002798 0.016961 0.004508 0.009447 0.008071 0.013010 0.004102 0.008634 0.006278 0.015227
Nuclear Periphery 0.001305 0.001295 0.001547 0.002555 0.000848 0.001896 0.001541 0.002793 0.001013 0.001795 0.001543 0.002659
Nuclear Periphery Foci 0.000104 0.001325 0.001411 0.001242 0.000193 0.000631 0.000845 0.000541 0.000161 0.000748 0.001037 0.000934
Nucleolus 0.001400 0.002007 0.000625 0.001370 0.001002 0.001977 0.000791 0.003502 0.001146 0.001982 0.000734 0.002306
Nucleus 0.725010* 0.787564* 0.857770* 0.803250* 0.671598* 0.798134* 0.878134* 0.714719* 0.690896* 0.796358* 0.871208* 0.764395*
Peroxisomes 0.000641 0.002338 0.000217 0.000439 0.000916 0.000611 0.000120 0.000769 0.000817 0.000901 0.000153 0.000584
Vacuole 0.003734 0.010848 0.043422 0.013257 0.004570 0.006936 0.026192 0.015296 0.004268 0.007593 0.032052 0.014152
Vacuole Periphery 0.000244 0.000283 0.000170 0.001434 0.000116 0.000485 0.000103 0.003720 0.000162 0.000451 0.000125 0.002437

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.99 -9.87 -10.60 -0.01 4.48 -2.59 -12.73 -11.54 -7.41 1.36 -3.16 -15.84 -15.49 -5.71 4.17
Bud Neck -1.29 -2.50 -9.39 -3.63 -6.90 -5.13 -8.35 -15.33 -11.57 -12.60 -4.72 -7.60 -18.38 -11.65 -14.23
Bud Site -1.27 -1.48 -4.89 -0.99 0.52 -4.95 -4.97 -10.23 -5.45 -7.19 -4.70 -2.85 -12.13 -4.10 -2.78
Cell Periphery -5.93 -7.80 -10.97 0.55 3.79 -4.04 -7.81 -13.53 -0.10 0.25 -4.68 -10.87 -17.22 0.52 2.90
Cytoplasm 7.83 20.65 26.49 9.27 -1.36 19.56 35.97 35.80 15.07 -4.89 20.48 41.13 45.52 19.58 -4.06
Cytoplasmic Foci -1.44 9.02 9.70 5.63 1.05 7.20 14.64 13.66 7.29 -3.14 5.58 17.21 16.92 9.53 -1.22
Eisosomes -5.06 -8.83 -8.77 2.39 6.63 -1.34 -9.63 -10.68 -8.25 5.67 -4.45 -12.98 -13.59 -5.25 8.66
Endoplasmic Reticulum -8.96 -10.79 -17.95 2.95 8.29 -7.07 -14.55 -18.64 -5.08 10.83 -10.74 -17.48 -24.27 -4.85 12.77
Endosome -5.11 -5.81 -2.67 -0.86 -0.15 -2.89 -2.82 -3.19 -0.54 -1.37 -4.16 -5.82 -3.41 -1.09 -1.05
Golgi -1.29 0.24 -2.22 -0.35 -2.52 -0.92 1.67 -1.77 -1.34 -2.19 -1.40 1.66 -2.40 -1.28 -3.05
Lipid Particles -2.12 -1.28 4.32 3.44 5.39 0.81 0.42 1.52 0.37 0.97 -0.53 -0.60 5.01 3.82 5.39
Mitochondria 1.18 1.91 -0.08 -1.68 -4.02 -1.50 0.98 -3.48 -1.99 -7.92 -0.47 2.08 -2.41 -2.12 -8.20
Mitotic Spindle 1.81 -1.52 -9.22 -10.09 -2.87 -2.69 -0.87 -12.47 -11.56 -12.03 -2.34 -1.72 -15.50 -14.11 -11.01
None -1.58 2.14 -6.91 -5.35 -7.83 -4.36 -0.87 -9.66 -2.64 -7.33 -4.83 -0.14 -10.56 -2.90 -9.16
Nuclear Periphery -0.16 -5.21 -7.34 -5.77 -2.40 -2.57 -10.37 -12.17 -4.45 -6.01 -2.32 -11.45 -14.48 -4.10 -5.76
Nuclear Periphery Foci -4.81 -5.07 -3.29 0.46 1.26 -2.19 -5.70 -4.78 -2.27 -0.98 -3.35 -7.62 -5.26 -1.75 0.22
Nucleolus -0.89 1.59 -0.68 0.33 -4.17 -3.01 1.53 -6.42 -4.46 -6.94 -2.70 2.17 -5.90 -3.08 -7.99
Nucleus -4.19 -6.38 -12.32 -3.97 -0.28 -15.00 -18.01 -7.65 5.33 10.25 -14.76 -17.87 -16.22 -0.40 6.30
Peroxisomes -1.73 1.83 2.12 2.22 1.44 0.76 2.57 1.97 2.96 -2.56 -0.51 3.07 2.73 3.81 -1.74
Vacuole -5.84 -6.89 -12.18 -6.21 4.22 -3.32 -7.37 -13.59 -12.29 1.21 -5.38 -10.00 -18.07 -14.69 4.10
Vacuole Periphery -0.51 0.58 -1.31 -1.15 -2.09 -3.78 -0.07 -2.50 -1.12 -2.50 -3.38 0.56 -3.08 -0.87 -3.29