Standard name | |
---|---|
Description | Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; two functional forms of Hac1p are produced; translation initiation is repressed under non-stress conditions; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 4 | 1 | – | 3 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 7 | 3 | 9 | 21 | 10 |
Bud Neck | 0 | 0 | 2 | 1 | 0 | 0 | 6 | – | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 2 | 3 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 41 | 44 | 74 | 146 | 175 | 154 | 218 | – | 227 | 223 | 159 | 198 | 47 | 104 | 168 | 80 | 52 | 64 | 25 | 34 | 60 | 39 | 43 | 31 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 5 | 6 | 2 | 2 | 0 | 1 | 2 | 2 |
Endosome | 3 | 5 | 3 | 2 | 3 | 0 | 2 | – | 2 | 1 | 1 | 0 | 6 | 14 | 16 | 12 | 10 | 19 | 5 | 2 | 2 | 7 | 28 | 20 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 2 |
Mitochondria | 7 | 7 | 3 | 9 | 9 | 95 | 58 | – | 166 | 132 | 141 | 201 | 1 | 2 | 0 | 1 | 2 | 6 | 1 | 2 | 10 | 6 | 7 | 5 |
Nucleus | 106 | 97 | 99 | 34 | 32 | 28 | 31 | – | 44 | 54 | 44 | 55 | 179 | 114 | 72 | 55 | 50 | 16 | 154 | 162 | 121 | 111 | 118 | 98 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 4 | 4 | 1 | 6 | 6 | 14 | 11 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 3 | 1 | 1 | 2 | 3 | – | 5 | 2 | 2 | 3 | 0 | 1 | 1 | 3 | 2 | 6 | 6 | 7 | 9 | 14 | 42 | 46 |
Vac/Vac Membrane | 87 | 146 | 48 | 29 | 42 | 9 | 41 | – | 27 | 14 | 11 | 13 | 70 | 127 | 187 | 112 | 112 | 121 | 22 | 45 | 53 | 34 | 62 | 51 |
Unique Cell Count | 209 | 246 | 213 | 202 | 243 | 235 | 321 | 388 | 372 | 310 | 376 | 279 | 321 | 387 | 228 | 193 | 195 | 240 | 273 | 275 | 238 | 361 | 297 | |
Labelled Cell Count | 245 | 300 | 236 | 223 | 263 | 295 | 364 | 479 | 436 | 368 | 479 | 306 | 363 | 444 | 277 | 235 | 242 | 240 | 273 | 275 | 238 | 361 | 297 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.8 | 5.8 | 5.3 | 5.6 | 3.8 | 4.6 | – | 3.8 | 3.9 | 4.0 | 3.8 | 7.3 | 7.1 | 6.9 | 7.8 | 8.0 | 7.4 | 5.8 | 6.0 | 6.5 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.1 | 1.2 | 1.5 | 1.2 | 1.4 | – | 1.2 | 1.0 | 1.5 | 1.1 | 1.6 | 1.6 | 1.8 | 2.2 | 2.1 | 1.6 | 1.6 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.05 | -0.59 | -0.34 | – | -0.59 | -0.56 | -0.55 | -0.62 | 0.33 | 0.3 | 0.25 | 0.42 | 0.47 | 0.35 | -0.0 | 0.05 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7.7 | 8.0 | 6.5 | 7.5 | – | 5.6 | 5.9 | 3.7 | 4.2 | -4.6 | -0.6 | 2.1 | 0.1 | -1.7 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.9 | 1.8 | 2.2 | 2.2 | 3.7 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | 1.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -6.5 | -7.8 | -8.1 | -9.7 | – | -9.7 | -8.5 | -8.1 | -8.5 | 3.9 | -2.5 | -7.2 | -4.9 | -4.3 | -8.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.1 | -1.4 | -5.9 | -3.0 | – | -5.5 | -7.1 | -6.7 | -7.3 | 0.7 | 4.1 | 6.2 | 5.8 | 7.3 | 8.1 |