Standard name
Description Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.98 0.98 0.99 0.95 0.89 0.93 0.83 0.85 0.74 0.79 1.0 0.98 0.99 0.98 0.97 0.95 0.84 0.86 0.74 0.88 0.8 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.05 0.16 0.09 0.26 0.21 0.48 0.22 0 0 0 0 0 0 0 0 0.1 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 1 3 3
Bud 0 1 0 0 1 0 5 2 5 0 7 4 0 0 0 0 0 1 1 0 1 0 3 2
Bud Neck 2 1 5 4 13 7 3 17 5 7 2 11 1 3 0 0 0 0 0 2 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 3 3 1 0 0 1 1 2 1 5 6 0 1 3 4 4 0 0 3 0 1 1
Cytoplasm 158 135 175 202 285 256 313 227 249 209 192 206 137 206 175 122 170 130 131 128 118 85 144 148
Endoplasmic Reticulum 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 9 13 7 0 0 0 0 0 2
Endosome 0 0 0 0 0 0 2 0 1 0 1 6 0 2 1 0 2 0 1 2 2 0 2 1
Golgi 0 1 0 1 1 0 0 0 0 0 0 0 0 2 0 1 0 0 1 1 0 0 0 0
Mitochondria 20 5 0 1 5 13 55 21 78 51 124 58 0 0 0 1 3 5 4 5 16 3 8 7
Nucleus 0 0 0 1 1 0 0 0 4 0 3 5 0 1 0 3 2 1 0 0 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 3 2 2 2 0 6 5 1 6 0 0 0 0 0 0 3 1 2 0 3 4
Vac/Vac Membrane 1 1 0 2 0 2 3 0 0 1 0 2 0 0 1 0 4 5 2 3 1 0 2 3
Unique Cell Count 169 142 178 207 288 269 351 245 300 247 259 260 137 211 177 125 176 137 158 150 161 97 181 186
Labelled Cell Count 183 146 185 217 309 281 384 268 349 275 332 306 144 215 179 139 198 153 158 150 161 97 181 186


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.0 4.6 4.7 4.5 3.8 3.6 3.9 3.4 3.4 3.3 3.5 4.8 5.3 5.5 6.0 6.0 5.6 4.7 5.2 5.4
Std Deviation (1e-4) 0.6 1.2 1.7 2.5 1.8 2.1 2.1 1.4 1.8 1.2 2.2 1.4 1.4 2.3 2.7 1.5 1.7 1.4 2.0 2.6 2.0
Intensity Change (Log2) 0.03 -0.02 -0.27 -0.33 -0.24 -0.43 -0.44 -0.46 -0.39 0.06 0.22 0.26 0.4 0.39 0.3 0.03 0.2 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cak1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available