Standard name
Human Ortholog
Description Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.06 0 0 0.05 0 0.05 0.12 0.1 0.11 0.11 0.05 0.07 0.16 0.24 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0.1 0.05 0 0 0 0 0 0 0 0 0.36 0.18 0.08 0.08 0.1 0.05 0.05 0 0.13 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.05 0 0 0 0.07 0 0.06
Golgi 0.08 0 0.2 0.09 0.05 0 0 0 0 0 0 0 0.26 0.17 0.05 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.09 0.2 0.05 0 0.05 0.05 0.06 0.06 0.1 0.11 0.13 0.1 0 0 0 0 0.07 0.08 0 0 0 0.06 0.07 0.07
Nucleus 0.21 0.15 0.1 0.19 0.13 0.19 0.25 0.22 0.15 0.19 0.11 0.14 0.05 0.12 0.15 0.16 0.34 0.24 0 0 0 0 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Nucleolus 0.1 0 0 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.62 0.5 0.66 0.68 0.8 0.71 0.7 0.76 0.74 0.71 0.76 0.76 0.38 0.5 0.46 0.52 0.68 0.71 0.78 0.85 0.78 0.71 0.71 0.66
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 4 4 2 4 2 0 2 0 2 2 17 22 22 0 0 0 0 0 6 0 0 0
Bud 0 0 0 1 0 1 1 2 0 1 0 0 2 1 3 0 0 0 2 0 3 1 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 1 0 0 0 0 1 0 7 1 8 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 15 9 8 25 29 43 55 46 54 55 11 25 50 6 5 4 2 0 0 0 0 1
Endoplasmic Reticulum 7 0 27 14 4 6 3 3 3 0 5 1 75 65 24 2 6 2 9 2 40 0 0 1
Endosome 1 0 1 2 3 14 1 2 1 1 1 2 2 11 22 2 3 2 3 0 1 22 12 29
Golgi 11 0 53 23 15 1 7 3 5 1 0 2 53 62 16 0 0 2 4 0 4 10 6 17
Mitochondria 13 4 12 6 14 23 39 48 46 49 60 48 0 2 1 0 4 3 6 0 9 20 37 35
Nucleus 30 3 27 49 38 91 172 186 72 87 54 71 10 44 45 4 20 9 6 2 0 8 30 16
Nuclear Periphery 3 0 2 0 0 6 9 6 4 8 5 6 0 1 2 1 0 0 0 0 2 7 22 35
Nucleolus 14 0 11 24 10 22 14 26 8 8 17 17 1 3 6 0 0 0 1 0 0 23 35 28
Peroxisomes 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 1 1 0 0 0 0 0 0 0 0 9 12 0 0 0 1 0 0 1 1 2
Vac/Vac Membrane 90 10 171 178 230 333 482 642 344 328 360 381 78 180 141 13 40 27 148 53 249 243 366 338
Unique Cell Count 145 20 261 263 288 467 692 840 467 460 473 500 206 363 305 25 59 38 190 63 319 343 520 516
Labelled Cell Count 177 20 323 312 326 529 759 961 540 529 559 585 256 426 352 28 78 49 190 63 319 343 520 516


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.0 15.0 28.0 32.7 39.5 34.2 35.0 33.0 33.8 32.4 29.4 31.9 23.0 15.5 11.6 6.1 6.6 8.1 32.5 30.7 32.1
Std Deviation (1e-4) 8.9 7.1 10.6 11.3 17.5 18.0 18.5 17.8 16.8 17.5 15.6 16.5 7.4 4.9 4.2 1.2 1.6 2.1 10.9 10.9 11.6
Intensity Change (Log2) – – – 0.23 0.5 0.29 0.33 0.24 0.27 0.21 0.07 0.19 -0.28 -0.85 -1.27 -2.19 -2.07 -1.79 0.22 0.13 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.5 2.8 3.2 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.7 -0.2 -1.0 -0.4 2.6 2.0 2.5 2.4 -0.2 0.6 4.0 0 0 0
Endoplasmic Reticulum -2.1 -4.5 -5.6 -7.8 -8.7 -6.3 -7.0 -5.9 -7.1 6.8 2.6 -1.0 0 0 0
Endosome 0 0 2.4 0 0 0 0 0 0 0 2.4 4.1 0 0 0
Golgi -3.8 -5.4 -9.9 -10.9 -12.8 -9.2 -9.8 -10.2 -10.1 1.4 -1.0 -5.5 0 -3.8 -2.2
Mitochondria -1.5 0.1 0.2 0.6 0.7 2.5 2.8 3.5 2.4 -3.1 -3.4 -3.4 0 0 0
Nucleus 2.7 1.0 3.2 4.9 4.2 1.9 3.0 0.4 1.5 -2.2 0.7 1.6 0 4.6 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 -0.5 0.3 -1.9 -0.9 -2.0 -2.0 -0.4 -0.6 -2.5 -2.8 -1.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole 0.5 3.8 1.6 1.2 3.5 2.3 1.6 3.1 3.1 -5.9 -4.0 -4.6 -1.3 0.3 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.6783 31.8271 28.0587 29.1503 27.9609 29.7961 32.9923 34.4411 27.9314 27.2646 24.2402 30.0409 31.1079 35.7416 33.828 27.8262 37.1343 30.5432
Actin 0.0062 0.0001 0.0069 0 0 0.0002 0.0197 0.0001 0.0003 0.0001 0.0321 0.0001 0.0059 0.0006 0.006 0 0 0.0019
Bud 0.0003 0.0002 0.0003 0.0003 0.0003 0.0003 0.0006 0.0005 0.0006 0.0002 0.0016 0.0003 0.0008 0.0006 0.0006 0.0002 0.0003 0.0013
Bud Neck 0.001 0.0005 0.001 0.0008 0.0005 0.0005 0.0019 0.0006 0.0015 0.0004 0.0014 0.0007 0.0008 0.0023 0.0014 0.0007 0.0016 0.0014
Bud Periphery 0.0012 0.0009 0.0013 0.0012 0.0012 0.0014 0.0022 0.0017 0.0029 0.0011 0.004 0.0022 0.0025 0.0026 0.0026 0.0008 0.0011 0.0076
Bud Site 0.0008 0.0005 0.0011 0.0007 0.0004 0.0003 0.0034 0.0021 0.005 0.0004 0.0114 0.0004 0.0018 0.0025 0.0017 0.0003 0.0038 0.0012
Cell Periphery 0.2053 0.1547 0.1531 0.1071 0.116 0.1441 0.2695 0.2434 0.218 0.1968 0.1528 0.2094 0.1875 0.169 0.1646 0.1425 0.2132 0.1689
Cytoplasm 0.0019 0.0006 0.0049 0.0005 0.001 0.0007 0.0046 0.0009 0.0016 0.0004 0.0013 0.0006 0.0036 0.0005 0.0004 0.0003 0.0004 0.0018
Cytoplasmic Foci 0.0028 0.0005 0.0007 0.0004 0.0003 0.0005 0.0079 0.0004 0.0042 0.0009 0.006 0.0013 0.0102 0.0016 0.0048 0.0009 0.0018 0.0016
Eisosomes 0.0003 0 0.0002 0 0 0 0.0004 0.0001 0.0002 0 0.0002 0 0.0003 0.0002 0.0003 0 0 0
Endoplasmic Reticulum 0.0021 0.0024 0.0134 0.0004 0.0008 0.0028 0.0051 0.007 0.0041 0.0013 0.0022 0.0029 0.0062 0.0028 0.0012 0.0004 0.0004 0.0022
Endosome 0.0103 0.0077 0.0103 0.0058 0.0091 0.0139 0.0123 0.0102 0.01 0.0095 0.0348 0.0125 0.0365 0.0221 0.0093 0.0151 0.0179 0.0235
Golgi 0.0011 0.0008 0.0023 0.0003 0.0002 0.0034 0.0034 0.0007 0.0013 0.0009 0.0037 0.0047 0.0157 0.0059 0.0048 0.0006 0.0021 0.0081
Lipid Particles 0.033 0.0123 0.0206 0.0329 0.0065 0.016 0.0538 0.0191 0.0375 0.0681 0.0511 0.0191 0.0683 0.0256 0.08 0.0694 0.083 0.0276
Mitochondria 0.0055 0.0019 0.0142 0.0008 0.0003 0.0043 0.016 0.0115 0.0189 0.0025 0.0215 0.007 0.0702 0.018 0.0557 0.0007 0.0015 0.0184
None 0.0013 0 0.0003 0 0 0 0.0024 0.0001 0.0036 0 0.0002 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.0024 0.0028 0.0031 0.0004 0.0006 0.0016 0.0051 0.0023 0.0023 0.0007 0.0011 0.0014 0.0021 0.0016 0.0008 0.0004 0.0004 0.0013
Nucleolus 0.0295 0.009 0.0048 0.0099 0.0044 0.0041 0.0077 0.0035 0.017 0.0051 0.0195 0.0116 0.0151 0.0185 0.0126 0.0111 0.0316 0.0097
Nucleus 0.0096 0.0031 0.0025 0.0026 0.0019 0.002 0.0025 0.0017 0.0034 0.0014 0.0033 0.0026 0.0043 0.0037 0.003 0.0025 0.0033 0.0029
Peroxisomes 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001 0.0033 0.0001 0.0009 0.0001 0.0024 0.0002 0.0035 0.0013 0.0057 0.0002 0.0005 0.0004
Punctate Nuclear 0.0008 0 0.0002 0 0 0 0.0027 0 0.0007 0 0.0004 0 0.0015 0 0 0 0 0.0001
Vacuole 0.5819 0.6791 0.6291 0.6952 0.7642 0.6602 0.4948 0.5852 0.5546 0.5996 0.583 0.6179 0.4351 0.5516 0.4746 0.6248 0.5552 0.5984
Vacuole Periphery 0.1023 0.1228 0.1293 0.1404 0.0921 0.1437 0.0806 0.1088 0.1113 0.1104 0.0659 0.1052 0.1276 0.1689 0.1698 0.1288 0.0815 0.1218

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.0232 39.5768 140.0614 225.0721 91.0691 77.4338 69.6434 111.6684 145.7249 115.1523
Translational Efficiency 0.547 0.4934 0.8881 0.5523 0.7701 0.5996 0.4755 0.6262 0.759 0.678

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells
Localization
Cell Percentages vacuole (31%), ER (19%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ste2

Ste2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available