Standard name
Description Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.10 0.06 0 0 0.05 0 0.05 0.12 0.10 0.11 0.11 0.05 0.07 0.16 0.24 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0.10 0.05 0 0 0 0 0 0 0 0 0.36 0.18 0.08 0.08 0.10 0.05 0.05 0 0.13 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.05 0 0 0 0.07 0 0.06
Golgi 0.08 0 0.20 0.09 0.05 0 0 0 0 0 0 0 0.26 0.17 0.05 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.09 0.20 0.05 0 0.05 0.05 0.06 0.06 0.10 0.11 0.13 0.10 0 0 0 0 0.07 0.08 0 0 0 0.06 0.07 0.07
Nucleus 0.21 0.15 0.10 0.19 0.13 0.19 0.25 0.22 0.15 0.19 0.11 0.14 0.05 0.12 0.15 0.16 0.34 0.24 0 0 0 0 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Nucleolus 0.10 0 0 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.62 0.50 0.66 0.68 0.80 0.71 0.70 0.76 0.74 0.71 0.76 0.76 0.38 0.50 0.46 0.52 0.68 0.71 0.78 0.85 0.78 0.71 0.71 0.66
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 4 4 2 4 2 0 2 0 2 2 17 22 22 0 0 0 0 0 6 0 0 0
Bud 0 0 0 1 0 1 1 2 0 1 0 0 2 1 3 0 0 0 2 0 3 1 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 0 1 0 0 0 0 1 0 7 1 8 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 15 9 8 25 29 43 55 46 54 55 11 25 50 6 5 4 2 0 0 0 0 1
Endoplasmic Reticulum 7 0 27 14 4 6 3 3 3 0 5 1 75 65 24 2 6 2 9 2 40 0 0 1
Endosome 1 0 1 2 3 14 1 2 1 1 1 2 2 11 22 2 3 2 3 0 1 22 12 29
Golgi 11 0 53 23 15 1 7 3 5 1 0 2 53 62 16 0 0 2 4 0 4 10 6 17
Mitochondria 13 4 12 6 14 23 39 48 46 49 60 48 0 2 1 0 4 3 6 0 9 20 37 35
Nucleus 30 3 27 49 38 91 172 186 72 87 54 71 10 44 45 4 20 9 6 2 0 8 30 16
Nuclear Periphery 3 0 2 0 0 6 9 6 4 8 5 6 0 1 2 1 0 0 0 0 2 7 22 35
Nucleolus 14 0 11 24 10 22 14 26 8 8 17 17 1 3 6 0 0 0 1 0 0 23 35 28
Peroxisomes 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 1 1 0 0 0 0 0 0 0 0 9 12 0 0 0 1 0 0 1 1 2
Vac/Vac Membrane 90 10 171 178 230 333 482 642 344 328 360 381 78 180 141 13 40 27 148 53 249 243 366 338
Unique Cell Count 145 20 261 263 288 467 692 840 467 460 473 500 206 363 305 25 59 38 190 63 319 343 520 516
Labelled Cell Count 177 20 323 312 326 529 759 961 540 529 559 585 256 426 352 28 78 49 190 63 319 343 520 516

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Vacuole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.0 15.0 28.0 32.7 39.5 34.2 35.0 33.0 33.8 32.4 29.4 31.9 23.0 15.5 11.6 6.1 6.6 8.1 32.5 30.7 32.1
Std Deviation (1e-4) 8.9 7.1 10.6 11.3 17.5 18.0 18.5 17.8 16.8 17.5 15.6 16.5 7.4 4.9 4.2 1.2 1.6 2.1 10.9 10.9 11.6
Intensity Change (Log2) 0.23 0.5 0.29 0.33 0.24 0.27 0.21 0.07 0.19 -0.28 -0.85 -1.27 -2.19 -2.07 -1.79 0.22 0.13 0.2

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.5 2.8 3.2 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 -1.7 0 0 0.3 0 0 0 0 0.4 0.6 0 0 0
Endoplasmic Reticulum -2.1 -4.5 -5.6 -7.8 -8.7 -6.3 -7.0 -5.9 -7.1 6.8 2.6 -1.0 0 0 0
Endosome 0 0 2.4 0 0 0 0 0 0 0 2.4 4.1 0 0 0
Golgi -3.8 -5.4 -9.9 -10.9 -12.8 -9.2 -9.8 -10.2 -10.1 1.4 -1.0 -5.5 0 -3.8 -2.2
Mitochondria -1.5 0.1 0.2 0.6 0.7 2.5 2.8 3.5 2.4 -3.1 -3.4 -3.4 0 0 0
Nucleus 0 2.2 5.0 6.3 5.7 3.7 6.1 4.1 4.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 -0.5 0.3 -1.9 -0.9 -2.0 -2.0 -0.4 -0.6 -2.5 -2.8 -1.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole 3.3 7.5 3.6 2.6 2.5 -0.5 -1.3 -0.8 -1.9 -2.1 0.9 0.6 0.8 3.7 3.7