Standard name
Human Ortholog
Description GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.92 0.81 0.86 0.82 0.85 0.85 0.82 0.82 0.83 0.8 0.88 0.88 0.97 0.79 0.78 0.13 0.28 0.08 0.17 0.27 0.2
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.28 0.21 0.38 0.23 0.21 0.24
Endosome 0.06 0 0.09 0.08 0.07 0.06 0 0 0 0 0 0 0 0 0 0.06 0.06 0.08 0.09 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.12 0.18 0.28 0.3 0.33 0.05 0.05 0 0 0 0.09 0.06 0.13 0.14 0.09 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0.07 0 0.08 0 0 0 0 0 0 0 0 0 0.14 0.17 0.41 0.3 0.24 0.36 0.36 0.42
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 5 2 2 1 1 2 1 1 0 0 0 2 0 0 1 4 7 0 1 5
Bud 0 4 4 5 3 10 9 25 27 22 28 0 0 0 0 0 0 2 1 0 5 4
Bud Neck 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 5 2 1 3 1
Bud Site 0 0 0 0 1 1 2 0 0 1 1 0 0 0 0 0
Cell Periphery 0 0 0 2 3 3 1 1 5 4 3 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 290 212 240 197 252 385 483 614 518 412 480 38 38 170 34 14 52 77 27 35 108 82
Endoplasmic Reticulum 19 7 12 7 3 4 7 4 2 1 1 1 1 3 1 1 111 57 132 47 83 100
Endosome 20 4 28 18 22 27 17 26 6 1 9 1 1 3 1 1 22 22 30 12 11 10
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 12 1 2 2
Mitochondria 2 5 6 5 9 53 70 137 176 148 198 2 2 0 1 0 36 15 44 29 36 24
Nucleus 0 0 0 0 3 0 1 5 2 1 3 1 1 0 2 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 2 2 0 0 0 0 0 0 0 2 0 3 1 0 1
Nucleolus 0 0 0 0 3 0 0 3 3 1 1 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 2 0
SpindlePole 2 0 7 1 4 4 5 7 4 5 4 0 0 2 0 0 0 1 0 0 3 0
Vac/Vac Membrane 22 7 20 6 24 19 17 15 16 14 24 0 0 2 6 3 162 83 82 75 144 176
Unique Cell Count 341 231 298 228 307 455 565 749 631 499 599 43 43 176 43 18 398 278 352 210 407 416
Labelled Cell Count 360 241 323 244 330 507 614 842 763 611 752 43 43 182 45 20 398 278 352 210 407 416


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.6 5.2 5.3 5.2 4.2 4.1 4.1 3.4 3.5 3.4 5.4 5.4 6.6 4.8 5.5 6.4 6.7 6.8 7.0
Std Deviation (1e-4) 0.7 1.1 1.5 1.4 1.8 1.2 1.1 1.3 1.1 0.8 0.9 1.0 1.0 1.5 1.0 1.4 1.3 1.2 1.5 1.3
Intensity Change (Log2) 0.02 0.02 -0.29 -0.34 -0.34 -0.6 -0.55 -0.63 0.05 0.05 0.34 -0.1 0.08 0.3 0.36 0.39 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 0.5 1.5 1.9 0.5 0.6 0.7 -0.1 1.2 1.2 4.9 -0.2 -0.3
Endoplasmic Reticulum -0.6 -2.4 0 0 0 0 0 0 0 0 -1.4 0 0
Endosome -0.6 -1.0 0 0 0 0 0 0 0 0 -3.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.7 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 -1.0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 0.5 -1.5 -2.6 -3.8 -3.1 -2.6 -1.8 0 0 -2.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3322 1.6508 1.4507 1.5757 1.9094 1.39 1.8994 2.7634 2.553 2.2454 2.4187 2.3644 2.5651 2.0997 2.3453 1.8154 2.1117 2.0801
Actin 0.0258 0.001 0.0083 0.0789 0.0526 0.0063 0.0346 0.0008 0.0142 0.003 0.0155 0.0085 0.0023 0.0003 0.0296 0.0149 0.0011 0.0021
Bud 0.0007 0.0004 0.0005 0.0007 0.0008 0.0001 0.0006 0.0004 0.0004 0.0003 0.0001 0.0002 0.0001 0 0.0003 0.0002 0.0003 0.0001
Bud Neck 0.0036 0.0002 0.0019 0.0023 0.0018 0.0007 0.0018 0.0002 0.0009 0.0018 0.0007 0.0016 0.0004 0.0002 0.0003 0.0003 0.0012 0.0006
Bud Periphery 0.0012 0.0006 0.0007 0.0009 0.001 0.0002 0.0006 0.0004 0.0005 0.0006 0.0001 0.0003 0.0001 0 0.0002 0.0002 0.0002 0.0001
Bud Site 0.0054 0.0018 0.0018 0.0012 0.0038 0.0002 0.0041 0.0022 0.0055 0.0008 0.0004 0.0005 0.0003 0.0003 0.0008 0.0003 0.001 0.0001
Cell Periphery 0.0009 0.0005 0.0003 0.0002 0.0003 0.0001 0.0005 0.0006 0.0006 0.0004 0.0001 0.0002 0.0002 0.0002 0.0003 0.0004 0.0001 0.0002
Cytoplasm 0.314 0.4714 0.4837 0.2948 0.4133 0.5002 0.2395 0.4741 0.3599 0.2562 0.2449 0.4055 0.1376 0.2858 0.3843 0.4408 0.3872 0.4231
Cytoplasmic Foci 0.0802 0.0695 0.0875 0.1156 0.1067 0.0743 0.1454 0.1272 0.118 0.1446 0.1811 0.1284 0.0243 0.0448 0.0794 0.0817 0.1232 0.0835
Eisosomes 0.0005 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0406 0.0529 0.0411 0.0343 0.0202 0.0371 0.0563 0.031 0.0548 0.0117 0.0076 0.0495 0.1061 0.077 0.0451 0.0707 0.0306 0.073
Endosome 0.2131 0.1382 0.175 0.2686 0.1955 0.1406 0.3256 0.1461 0.2195 0.3761 0.3689 0.2026 0.2891 0.2272 0.1901 0.1207 0.2392 0.1382
Golgi 0.0176 0.0076 0.0141 0.0432 0.0344 0.0056 0.0466 0.0144 0.0204 0.0222 0.032 0.0135 0.0102 0.0082 0.0285 0.0244 0.0077 0.0085
Lipid Particles 0.0316 0.0085 0.0107 0.0124 0.0328 0.0118 0.0406 0.0122 0.0225 0.0169 0.0125 0.0187 0.0318 0.0132 0.0216 0.0061 0.0533 0.0079
Mitochondria 0.0093 0.0028 0.0047 0.0113 0.0087 0.005 0.0097 0.0084 0.0159 0.0196 0.0027 0.0052 0.0025 0.0006 0.0049 0.0027 0.0013 0.002
None 0.1718 0.1906 0.1288 0.0683 0.0876 0.1833 0.05 0.1551 0.1286 0.0944 0.0792 0.1339 0.16 0.3112 0.1744 0.1752 0.1113 0.2393
Nuclear Periphery 0.0295 0.0124 0.0085 0.0159 0.006 0.0065 0.0076 0.0028 0.0052 0.0024 0.0014 0.0048 0.0741 0.0078 0.0025 0.0156 0.0053 0.0042
Nucleolus 0.0019 0.0007 0.0005 0.0003 0.0007 0.0005 0.0011 0.0002 0.0003 0.0003 0.0002 0.0002 0.0064 0.0001 0.0001 0.0001 0.0004 0.0001
Nucleus 0.0127 0.0047 0.004 0.0033 0.0021 0.0034 0.0017 0.002 0.0015 0.0012 0.0019 0.0013 0.0875 0.001 0.0008 0.0023 0.0021 0.0016
Peroxisomes 0.0042 0.0009 0.0067 0.0171 0.008 0.0022 0.0139 0.0039 0.0117 0.0054 0.0332 0.0101 0.0007 0.0003 0.0241 0.0033 0.0066 0.0014
Punctate Nuclear 0.0083 0.0037 0.005 0.006 0.0084 0.0066 0.0058 0.0014 0.0027 0.0023 0.0014 0.0024 0.0417 0.0007 0.0014 0.0007 0.002 0.0019
Vacuole 0.0215 0.0283 0.0136 0.0202 0.0121 0.0129 0.0116 0.015 0.0145 0.0328 0.0139 0.0105 0.0203 0.0194 0.0088 0.0354 0.022 0.0106
Vacuole Periphery 0.0058 0.0034 0.0026 0.0042 0.0031 0.0023 0.0022 0.0015 0.0024 0.0068 0.0022 0.0022 0.0038 0.0015 0.0024 0.0039 0.0042 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6046 8.938 9.4749 9.1913 14.5839 14.9894 12.2648 10.4563 8.1303 17.1051
Translational Efficiency 0.5373 0.611 0.4186 0.3536 0.3883 0.5906 0.4765 0.3406 0.4945 0.3803

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
479 524 215 81 836 1391 2172 201 1315 1915 2387 282

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 824.84 851.95 971.33 1008.36 582.48 816.71 842.66 905.79 670.76 826.35 854.25 935.25
Standard Deviation 87.33 128.43 95.79 124.57 71.76 110.07 106.87 104.09 140.19 116.45 112.14 119.72
Intensity Change Log 2 0.046654 0.235847 0.289825 0.487615 0.532742 0.636968 0.245740 0.366216 0.443753

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000658 0.002577 0.001911 0.000457 0.000760 0.000843 0.000533 0.000294 0.000723 0.001318 0.000657 0.000341
Bud Neck 0.005106 0.008536 0.004137 0.002684 0.025352 0.008839 0.009618 0.002288 0.017977 0.008756 0.009125 0.002402
Bud Site 0.008772 0.007981 0.014052 0.005060 0.006033 0.006931 0.015718 0.008635 0.007030 0.007218 0.015568 0.007608
Cell Periphery 0.000238 0.000157 0.000449 0.000306 0.000321 0.000183 0.000187 0.000256 0.000291 0.000176 0.000210 0.000270
Cytoplasm 0.330854* 0.137495* 0.331053* 0.268363* 0.222504* 0.144671* 0.456775* 0.293442* 0.261971* 0.142707* 0.445451* 0.286239*
Cytoplasmic Foci 0.132348 0.367389* 0.129960 0.066686 0.387451* 0.277681* 0.115062 0.141766 0.294527* 0.302228* 0.116404 0.120201
Eisosomes 0.000162 0.000375 0.000139 0.000155 0.001284 0.000279 0.000119 0.000187 0.000875 0.000306 0.000121 0.000178
Endoplasmic Reticulum 0.012396 0.028449 0.026872 0.013034 0.006270 0.031832 0.028623 0.012480 0.008501 0.030907 0.028465 0.012640
Endosome 0.035679 0.115913 0.036744 0.010946 0.078712 0.139862 0.020104 0.028885 0.063037 0.133309 0.021603 0.023732
Golgi 0.003090 0.019176 0.001317 0.000116 0.054582 0.028556 0.001147 0.000475 0.035826 0.025990 0.001163 0.000372
Lipid Particles 0.026621 0.052170 0.020920 0.010017 0.092822 0.067570 0.037168 0.021430 0.068708 0.063356 0.035704 0.018152
Mitochondria 0.004939 0.002898 0.000391 0.000077 0.019587 0.009525 0.001431 0.000216 0.014252 0.007712 0.001337 0.000176
Mitotic Spindle 0.006265 0.006180 0.002808 0.000177 0.000323 0.005543 0.011064 0.003598 0.002487 0.005717 0.010321 0.002615
None 0.003368 0.000197 0.000867 0.000532 0.003486 0.000687 0.001853 0.002484 0.003443 0.000553 0.001765 0.001923
Nuclear Periphery 0.007574 0.000721 0.003999 0.002622 0.000406 0.002659 0.012535 0.004081 0.003017 0.002129 0.011766 0.003662
Nuclear Periphery Foci 0.010376 0.003650 0.071126 0.022138 0.000506 0.002005 0.019850 0.023362 0.004101 0.002455 0.024469 0.023011
Nucleolus 0.000922 0.000459 0.000744 0.000180 0.001232 0.000906 0.001508 0.001177 0.001119 0.000784 0.001439 0.000891
Nucleus 0.109160 0.009262 0.121463 0.060319 0.026872 0.020508 0.151408* 0.095832 0.056846 0.017431 0.148710* 0.085631
Peroxisomes 0.005529 0.023020 0.003397 0.000643 0.021434 0.011644 0.002637 0.001266 0.015641 0.014757 0.002705 0.001087
Vacuole 0.291830* 0.209648* 0.226738* 0.535356* 0.045696 0.229471* 0.111989 0.357419* 0.135352 0.224047* 0.122324 0.408529*
Vacuole Periphery 0.004115 0.003746 0.000912 0.000131 0.004367 0.009805 0.000670 0.000425 0.004275 0.008147 0.000692 0.000341

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.76 -2.56 0.52 3.03 3.02 -0.40 1.19 3.85 2.45 0.96 -2.40 0.32 3.08 3.88 1.44
Bud Neck -2.30 0.98 2.16 3.93 1.15 8.02 7.25 11.58 10.93 10.16 6.54 5.76 11.60 10.82 9.86
Bud Site 0.08 -1.36 1.16 1.67 2.18 -0.73 -4.89 -0.73 -0.42 1.76 -0.37 -4.45 -0.34 -0.16 2.53
Cell Periphery 2.47 -5.80 -0.96 -2.72 3.78 3.58 5.62 2.96 -1.49 -2.97 3.89 4.34 1.82 -2.46 -2.22
Cytoplasm 13.06 0.02 3.28 -3.05 3.06 8.82 -23.91 -3.91 -8.53 8.63 15.13 -20.69 -0.97 -8.76 10.48
Cytoplasmic Foci -21.23 0.80 9.76 29.26 6.45 12.54 34.83 24.10 15.79 -0.73 -1.06 25.99 21.54 25.20 2.16
Eisosomes -2.06 1.48 1.09 2.23 0.05 11.80 13.92 13.23 5.34 -3.75 9.07 13.54 12.74 4.50 -3.50
Endoplasmic Reticulum -3.60 -4.07 1.01 3.89 4.28 -9.56 -10.84 -2.03 6.52 6.07 -9.76 -10.59 -1.12 7.62 7.35
Endosome -13.01 -0.08 7.28 20.58 6.31 -10.82 14.68 12.18 25.08 -2.39 -16.19 13.75 13.03 30.97 0.65
Golgi -7.58 1.61 2.93 9.76 2.67 6.84 16.47 16.69 13.83 3.19 3.68 15.70 16.08 16.26 4.14
Lipid Particles -5.22 2.03 6.98 9.87 5.60 4.87 11.56 12.79 11.02 4.52 1.47 9.53 12.98 13.87 6.36
Mitochondria 0.83 2.62 2.81 2.59 2.67 4.71 9.35 10.04 9.80 5.03 4.12 9.23 10.18 10.00 5.31
Mitotic Spindle -0.23 0.78 2.10 2.54 2.57 -4.22 -7.43 -1.76 0.99 3.32 -2.34 -4.84 -0.09 1.98 4.28
None 1.48 1.11 1.29 -2.00 1.49 2.18 1.34 0.85 -1.69 -0.47 2.59 1.50 1.30 -1.83 -0.05
Nuclear Periphery 5.33 2.66 4.04 -2.62 2.49 -4.35 -12.90 -3.14 -1.07 5.62 1.38 -8.89 -0.49 -1.42 6.84
Nuclear Periphery Foci 4.07 -8.74 -1.79 -4.16 7.32 -6.52 -16.88 -6.25 -5.80 -0.44 2.64 -14.93 -6.11 -6.89 1.54
Nucleolus 3.44 1.38 6.07 5.65 6.40 1.62 -1.33 0.75 -0.42 1.94 2.33 -1.79 1.56 0.05 2.94
Nucleus 15.25 -1.34 5.40 -6.02 5.53 2.16 -29.16 -7.31 -8.34 6.55 12.14 -21.15 -3.51 -9.72 9.41
Peroxisomes -5.90 1.35 3.34 8.48 3.38 5.80 13.29 14.62 11.22 5.72 0.55 11.94 13.96 13.82 7.12
Vacuole 6.46 3.25 -8.97 -12.15 -10.49 -27.64 -18.45 -19.24 -8.64 -14.81 -10.61 1.02 -17.70 -13.22 -18.80
Vacuole Periphery -0.03 2.06 2.61 10.56 3.28 -7.89 11.17 12.01 15.61 3.07 -5.74 6.45 7.11 17.30 4.84