Standard name
Description Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 – 0.94 0.8 – 0.71 0.58 0.69 0.51 0.54 0.99 0.98 0.98 0.95 0.95 0.94 0.87 0.88 0.52 0.77 0.83 0.82
Endoplasmic Reticulum 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0.06 0.1 0.07 0 0 0 0 0 0
Endosome 0 0 0 – 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.06 0 – 0 0.47 – 0.53 0.72 0.54 0.68 0.63 0 0 0 0 0 0 0.05 0 0.25 0.08 0.06 0.05
Nucleus 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 – 0 0 0 2 0 0 0 0 0 0 0 3 1 3 0 3 1
Bud 1 0 0 – 0 1 – 4 1 8 9 8 0 1 1 0 0 1 3 1 1 0 7 2
Bud Neck 1 0 3 – 0 0 – 3 0 1 0 0 0 0 0 0 1 3 1 0 0 0 3 4
Bud Site 0 0 0 – 0 0 – 1 0 0 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 – 0 0 – 2 1 1 2 2 0 1 0 6 3 10 0 0 2 0 2 1
Cytoplasm 306 106 71 – 16 122 – 119 108 143 97 113 264 178 191 260 266 343 281 107 45 109 276 180
Endoplasmic Reticulum 2 1 1 – 0 0 – 0 0 0 0 0 1 1 2 16 27 25 0 0 1 1 0 0
Endosome 1 0 0 – 1 0 – 0 0 0 0 0 0 0 0 1 2 4 4 0 1 2 2 3
Golgi 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 1 3 0 1 0 2 2 0
Mitochondria 15 6 3 – 0 71 – 89 134 112 129 132 1 2 1 6 11 12 15 3 21 11 18 11
Nucleus 0 0 0 – 0 0 – 2 1 2 0 1 0 3 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 – 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 – 0 0 – 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 – 0 0 0 0 0 0 1 0 3 0 2 1 0 0 0 0 0
SpindlePole 0 0 0 – 0 1 – 2 1 3 2 14 0 1 0 3 0 0 4 1 0 0 6 2
Vac/Vac Membrane 1 0 0 – 0 1 – 0 1 2 1 0 0 0 0 4 7 8 3 2 2 3 3 8
Unique Cell Count 313 109 73 17 152 168 185 207 190 208 266 181 194 274 280 366 325 122 87 143 332 220
Labelled Cell Count 328 115 80 17 196 222 247 272 245 273 266 188 196 301 320 412 325 122 87 143 332 220


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.8 3.7 – 4.0 3.1 – 2.8 2.4 2.7 2.4 2.4 4.3 4.4 4.5 5.7 5.2 5.6 4.4 4.4 4.4
Std Deviation (1e-4) 0.6 0.9 2.7 – 2.0 2.2 – 0.8 0.8 1.1 0.9 0.4 1.1 1.4 1.8 2.1 1.7 2.2 2.2 1.6 1.6
Intensity Change (Log2) – – – – 0.1 -0.29 – -0.43 -0.67 -0.5 -0.63 -0.65 0.19 0.23 0.26 0.61 0.46 0.58 0.24 0.24 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9804 -0.662 -0.9029 -1.091 -0.3673 -0.787 -0.7595 -1.1772 -1.1222 -1.2946 -1.1236 -1.1067 0.7965 -0.1002 0.2331 0.0994 1.3691 0.1239
Actin 0.0092 0.0003 0.0095 0.0103 0.0092 0.0004 0.0534 0.0002 0.0007 0.0002 0.0004 0.0101 0.0081 0.0006 0.0041 0.003 0.0009 0
Bud 0.0003 0.003 0.001 0.0001 0.0006 0.0009 0.0012 0.0041 0.0012 0.0069 0.0001 0.0004 0.0373 0.0072 0.0009 0 0.0003 0
Bud Neck 0.0003 0.0009 0.0002 0.0014 0.0016 0.0002 0.0048 0.0003 0.0001 0 0.0001 0.0006 0.0059 0.0001 0.0002 0 0.0045 0
Bud Periphery 0.0006 0.0031 0.0016 0.0001 0.0014 0.0004 0.0016 0.0012 0.0004 0.005 0.0002 0.0004 0.0134 0.0067 0.001 0 0.0004 0
Bud Site 0.0006 0.0064 0.002 0.0007 0.0047 0.0001 0.0088 0.0115 0.0012 0.0004 0.0001 0.0014 0.0254 0.0029 0.0063 0.0001 0.0078 0
Cell Periphery 0.0004 0.0004 0.0001 0.0001 0.001 0.0001 0.0004 0.0003 0.0001 0.0001 0.0001 0.0001 0.0009 0.0002 0.0001 0 0.0003 0
Cytoplasm 0.0115 0.05 0.035 0.0158 0.0203 0.0633 0.0197 0.0184 0.0234 0.0191 0.0227 0.0358 0.0142 0.0224 0.0231 0.0717 0.0087 0.0372
Cytoplasmic Foci 0.0059 0.016 0.0161 0.0038 0.0375 0.0044 0.0335 0.0033 0.0044 0.003 0.0034 0.0107 0.0668 0.0015 0.014 0.0058 0.0647 0.0165
Eisosomes 0.0007 0.0002 0.0003 0.0002 0.0004 0.0001 0.0005 0.0005 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.001 0.0009 0.0009 0.0002 0.0075 0.0008 0.0019 0.0003 0.0014 0.0004 0.0043 0.0003 0.0018 0.0008 0.0007 0.0153 0.0002 0.0004
Endosome 0.0057 0.0044 0.0033 0.0011 0.0362 0.0039 0.018 0.0002 0.006 0.0004 0.0108 0.0048 0.0285 0.0001 0.0103 0.0059 0.038 0.0151
Golgi 0.0011 0.0003 0.0021 0.0032 0.0363 0.0006 0.0091 0 0.0016 0 0.0006 0.0035 0.01 0 0.0029 0.0016 0.0276 0.0004
Lipid Particles 0.0074 0.0018 0.0034 0.0061 0.0219 0.0002 0.0168 0.0003 0.0007 0.0002 0.0004 0.0064 0.0361 0.0001 0.0047 0.0005 0.0788 0.0001
Mitochondria 0.0062 0.0039 0.0059 0.018 0.0445 0.0019 0.0082 0.0005 0.0011 0.0011 0.0017 0.0009 0.0308 0.0007 0.0042 0.0002 0.1172 0.0001
None 0.9203 0.8873 0.9084 0.9215 0.7211 0.9149 0.7888 0.9515 0.9335 0.9092 0.8402 0.9078 0.6334 0.9522 0.9192 0.894 0.5909 0.9292
Nuclear Periphery 0.0064 0.0011 0.0005 0.0001 0.009 0.0003 0.0025 0.0004 0.0014 0.0008 0.0305 0.0003 0.0011 0.0004 0.001 0.0006 0.0001 0.0001
Nucleolus 0.0089 0.0025 0.0002 0.0001 0.0021 0.0002 0.0008 0.0016 0.0009 0.0018 0.0011 0.0005 0.0026 0.0003 0.0006 0 0.0113 0
Nucleus 0.0042 0.0024 0.0004 0.0003 0.0078 0.0009 0.0046 0.0025 0.0097 0.0479 0.071 0.001 0.0018 0.0006 0.0007 0.0002 0.001 0.0001
Peroxisomes 0.002 0.0032 0.0069 0.0161 0.0185 0.0001 0.0187 0.0001 0.0076 0.0002 0.0001 0.0066 0.0703 0.0001 0.0026 0.0001 0.0391 0.0001
Punctate Nuclear 0.0039 0.0021 0.0007 0.0005 0.0129 0.0009 0.0034 0.0021 0.0033 0.0024 0.0004 0.0076 0.0085 0.0026 0.0016 0.0002 0.0038 0.0001
Vacuole 0.0023 0.0087 0.0011 0.0002 0.0035 0.0034 0.0024 0.0005 0.0008 0.0004 0.0061 0.0005 0.0022 0.0002 0.0013 0.0003 0.0033 0.0004
Vacuole Periphery 0.0008 0.0014 0.0004 0.0001 0.0018 0.002 0.0009 0.0001 0.0002 0.0002 0.0056 0.0001 0.0005 0 0.0003 0.0001 0.0012 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.332 53.4287 28.9724 38.9296 50.6675 22.9749 43.6329 45.5763 36.6007 38.4973
Translational Efficiency 1.805 1.7304 2.0223 1.5162 1.1763 2.1288 1.8063 1.698 1.635 1.4213

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘