Standard name
Description Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.94 0.8 0.71 0.58 0.69 0.51 0.54 0.99 0.98 0.98 0.95 0.95 0.94 0.87 0.88 0.52 0.77 0.83 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.07 0 0 0 0 0 0
Endosome 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.06 0 0 0.47 0.53 0.72 0.54 0.68 0.63 0 0 0 0 0 0 0.05 0 0.25 0.08 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 1 3 0 3 1
Bud 1 0 0 0 1 4 1 8 9 8 0 1 1 0 0 1 3 1 1 0 7 2
Bud Neck 1 0 3 0 0 3 0 1 0 0 0 0 0 0 1 3 1 0 0 0 3 4
Bud Site 0 0 0 0 0 1 0 0 3 2 0 0 0 0 0 0
Cell Periphery 1 0 2 0 0 2 1 1 2 2 0 1 0 6 3 10 0 0 2 0 2 1
Cytoplasm 306 106 71 16 122 119 108 143 97 113 264 178 191 260 266 343 281 107 45 109 276 180
Endoplasmic Reticulum 2 1 1 0 0 0 0 0 0 0 1 1 2 16 27 25 0 0 1 1 0 0
Endosome 1 0 0 1 0 0 0 0 0 0 0 0 0 1 2 4 4 0 1 2 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 1 0 2 2 0
Mitochondria 15 6 3 0 71 89 134 112 129 132 1 2 1 6 11 12 15 3 21 11 18 11
Nucleus 0 0 0 0 0 2 1 2 0 1 0 3 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 2 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 1 3 2 14 0 1 0 3 0 0 4 1 0 0 6 2
Vac/Vac Membrane 1 0 0 0 1 0 1 2 1 0 0 0 0 4 7 8 3 2 2 3 3 8
Unique Cell Count 313 109 73 17 152 168 185 207 190 208 266 181 194 274 280 366 325 122 87 143 332 220
Labelled Cell Count 328 115 80 17 196 222 247 272 245 273 266 188 196 301 320 412 325 122 87 143 332 220


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.8 3.7 4.0 3.1 2.8 2.4 2.7 2.4 2.4 4.3 4.4 4.5 5.7 5.2 5.6 4.4 4.4 4.4
Std Deviation (1e-4) 0.6 0.9 2.7 2.0 2.2 0.8 0.8 1.1 0.9 0.4 1.1 1.4 1.8 2.1 1.7 2.2 2.2 1.6 1.6
Intensity Change (Log2) 0.1 -0.29 -0.43 -0.67 -0.5 -0.63 -0.65 0.19 0.23 0.26 0.61 0.46 0.58 0.24 0.24 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gna1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available