Standard name
Human Ortholog
Description Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.14 0.14 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.06 0 0.14 0.11 0.18 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.9 0.94 0.94 0.94 0.87 0.89 0.85 0.83 0.76 0.68 0.74 0.93 0.89 0.84 0.75 0.73 0.78 0.83 0.91 0.91 0.82 0.66 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.31 0.14 0.07 0.09 0.07 0.19 0.18 0.18 0.33 0.34 0.36 0.37 0.3 0.19 0.27 0.13 0.14 0.14 0.11 0.06 0 0.14 0.24 0.23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 1 0 0 2 0 5 4 0 1 5 0 0 0 0 1 0 0 0 1 1 3 2
Bud Neck 0 0 0 0 1 0 1 0 0 2 2 7 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 2 2 7 8 4 17 14 10 10 1 0 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 4 9 12 9 15 20 8 19 16 8 7 19 36 32 19 15 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 2 2 2 0 0 0 0 0 0
Endosome 0 0 2 0 1 0 0 0 0 0 0 0 0 2 1 4 1 0 3 0 2 0 3 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 2
Mitochondria 7 5 4 7 3 43 33 25 69 66 113 64 5 9 5 3 4 3 1 0 3 0 2 1
Nucleus 241 157 269 308 429 424 511 519 417 453 423 420 254 338 387 170 102 135 225 160 280 203 250 283
Nuclear Periphery 0 0 1 0 0 1 8 5 4 8 10 3 0 0 1 2 0 0 0 0 0 0 0 0
Nucleolus 81 24 19 28 34 91 105 107 165 205 224 211 83 72 124 29 20 25 30 10 12 35 91 100
Peroxisomes 0 0 1 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 3 1 3 3 6 0 4 1 5 0 1 3 1 1 1 0 0 0 0 1 1
Vac/Vac Membrane 1 1 1 1 1 4 1 6 1 8 7 4 0 0 2 4 3 10 6 2 6 5 26 30
Unique Cell Count 265 175 285 326 458 488 573 609 502 597 620 565 273 380 461 227 140 173 271 176 310 249 383 438
Labelled Cell Count 335 192 302 359 486 584 685 698 685 779 808 737 350 441 563 249 153 191 271 176 310 249 383 438


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.6 7.9 7.4 8.1 6.6 6.7 7.1 6.1 6.3 6.0 6.1 8.9 8.6 8.6 11.5 10.7 11.4 8.0 8.6 9.0
Std Deviation (1e-4) 2.1 2.1 1.3 1.5 1.7 1.5 1.4 1.5 1.2 1.5 1.6 1.5 2.1 2.2 2.3 3.5 3.2 2.9 1.6 1.9 1.8
Intensity Change (Log2) – – – -0.1 0.03 -0.26 -0.24 -0.16 -0.38 -0.33 -0.38 -0.38 0.17 0.12 0.12 0.55 0.44 0.53 0.02 0.13 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.1 0 1.1 1.6 0 1.6 1.1 0 1.0 2.5 3.8 5.6 5.2 3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 0 4.2 3.0 2.1 5.7 5.0 7.0 5.0 0 0.9 0 0 0 0
Nucleus 0 -0.4 -3.3 -2.5 -3.9 -4.6 -6.7 -8.6 -7.0 -0.7 -2.5 -4.3 -6.3 -6.3 -5.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 0.4 4.6 4.6 4.4 8.3 8.8 9.3 9.5 7.3 4.6 6.8 2.4 2.6 2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.148 3.673 3.6655 3.0783 2.4621 3.4599 4.9392 5.0146 4.615 4.1399 4.2069 4.2123 5.4486 5.3065 4.5819 4.025 4.3585 4.5511
Actin 0.0056 0 0.012 0.0153 0.009 0.0078 0.0028 0.0002 0.0014 0.0024 0.0295 0.0001 0.0188 0 0.0046 0.0116 0 0.0016
Bud 0.0006 0 0.0002 0.0003 0.0002 0.001 0.0002 0.0001 0.0003 0.0001 0.0022 0.0001 0.0003 0 0.0002 0.0009 0 0.0001
Bud Neck 0.0021 0 0.0009 0.0019 0.0004 0.0005 0.0003 0 0.0001 0.0001 0.0008 0.0004 0.0004 0 0.0001 0.0005 0.0003 0.0005
Bud Periphery 0.0015 0 0.0002 0.0004 0.0003 0.0031 0.0004 0.0002 0.0005 0.0002 0.0028 0.0004 0.0009 0 0.0003 0.0009 0 0.0001
Bud Site 0.0041 0 0.0028 0.0034 0.0004 0.0009 0.0008 0.0002 0.0011 0.0002 0.0047 0.0001 0.001 0 0.0027 0.003 0 0.0001
Cell Periphery 0.0007 0 0.0001 0.0003 0.0001 0.0002 0.0002 0 0.0001 0 0.0001 0.0005 0.0002 0 0.0002 0.0001 0 0.0011
Cytoplasm 0.0013 0 0.0011 0.0002 0.0028 0.0002 0.0046 0 0.0003 0.0003 0.0019 0 0.0012 0 0 0.0054 0 0.0135
Cytoplasmic Foci 0.003 0 0.0044 0.0055 0.0172 0.0012 0.0118 0 0.0117 0.0151 0.013 0 0.0118 0 0.0008 0.0086 0 0.0004
Eisosomes 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0019 0 0.001 0.0002 0.0012 0.0007 0.0026 0 0.0003 0.0007 0.0011 0 0.0008 0 0 0.015 0 0.0001
Endosome 0.0139 0 0.0074 0.0088 0.016 0.0036 0.0174 0.0002 0.0075 0.0214 0.0377 0.0001 0.0072 0 0.001 0.0276 0 0.0027
Golgi 0.0059 0 0.0077 0.0085 0.0135 0.003 0.0049 0.0001 0.0024 0.0078 0.0138 0 0.0043 0 0.0011 0.0044 0 0.0029
Lipid Particles 0.0104 0 0.005 0.0153 0.1037 0.0083 0.0123 0 0.0124 0.0174 0.0063 0.0003 0.0188 0 0.0022 0.0031 0 0.0029
Mitochondria 0.0117 0 0.004 0.0118 0.0533 0.0088 0.0071 0.01 0.009 0.0038 0.0737 0.0055 0.0092 0.0001 0.0163 0.0023 0.0002 0.0023
None 0.003 0 0.0012 0.0001 0.0009 0.0002 0.0048 0 0.0002 0.0005 0.0005 0 0.0013 0 0.0001 0.0025 0 0.0001
Nuclear Periphery 0.0122 0.0012 0.0038 0.0003 0.0052 0.0025 0.0235 0.0002 0.0032 0.0012 0.0015 0.0005 0.0034 0.0002 0.0001 0.0139 0.0005 0.0007
Nucleolus 0.0462 0.0261 0.016 0.0132 0.1468 0.1239 0.0274 0.0109 0.0142 0.0078 0.1016 0.1152 0.0248 0.0195 0.0182 0.0304 0.185 0.0755
Nucleus 0.849 0.9673 0.9251 0.9036 0.6082 0.8275 0.8378 0.9774 0.926 0.8921 0.6991 0.8749 0.8617 0.98 0.9483 0.8574 0.8129 0.8941
Peroxisomes 0.0011 0 0.0018 0.0075 0.0125 0.0005 0.0012 0 0.0039 0.0266 0.0039 0.0001 0.0102 0 0.0019 0.0031 0 0.0006
Punctate Nuclear 0.0163 0.0053 0.0044 0.0006 0.0057 0.0036 0.035 0.0002 0.0018 0.0012 0.0033 0.0002 0.0199 0.0001 0.0002 0.0058 0.0009 0.0003
Vacuole 0.0056 0 0.0004 0.0021 0.0015 0.0011 0.0028 0.0001 0.0023 0.0005 0.0015 0.0008 0.0016 0 0.0009 0.0018 0 0.0001
Vacuole Periphery 0.0037 0 0.0004 0.0005 0.001 0.0011 0.0021 0.0001 0.0012 0.0005 0.0009 0.0007 0.0019 0 0.0007 0.0015 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9416 14.2334 8.4495 16.4742 17.2031 14.322 16.4302 16.8363 15.6332 17.7483
Translational Efficiency 1.8641 1.4974 2.2739 1.06 1.2021 1.4807 1.2053 1.1482 1.3929 1.1959

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
125 179 416 23 1713 490 178 552 1838 669 594 575

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 786.85 1118.17 1386.32 1191.56 786.11 958.72 1293.51 1461.83 786.16 1001.38 1358.51 1451.02
Standard Deviation 119.11 176.91 213.62 318.27 116.16 175.39 163.51 168.30 116.37 189.44 204.40 184.53
Intensity Change Log 2 — 0.506979 0.817100 0.598691 — 0.286378 0.718488 0.894972 — 0.400943 0.768659 0.754355

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003121 0.002662 0.000970 0.004207 0.000309 0.000493 0.001395 0.000136 0.000500 0.001073 0.001097 0.000298
Bud Neck 0.004427 0.016416 0.018849 0.045220 0.009673 0.006833 0.010073 0.025906 0.009316 0.009397 0.016219 0.026679
Bud Site 0.000803 0.004578 0.001602 0.070581 0.001526 0.000988 0.001232 0.001260 0.001476 0.001949 0.001491 0.004033
Cell Periphery 0.000060 0.000165 0.000037 0.001405 0.000167 0.000042 0.000037 0.000669 0.000159 0.000075 0.000037 0.000698
Cytoplasm 0.000195 0.042326 0.000109 0.078251 0.008985 0.000161 0.000071 0.004766 0.008387 0.011443 0.000097 0.007705
Cytoplasmic Foci 0.001093 0.019481 0.000160 0.027710 0.001536 0.001054 0.000073 0.000004 0.001506 0.005984 0.000134 0.001112
Eisosomes 0.000029 0.000047 0.000096 0.000112 0.000024 0.000052 0.000109 0.000042 0.000024 0.000050 0.000100 0.000045
Endoplasmic Reticulum 0.002016 0.003232 0.000874 0.003860 0.003018 0.001162 0.001574 0.001451 0.002950 0.001716 0.001084 0.001548
Endosome 0.000211 0.004680 0.000028 0.015907 0.000405 0.000236 0.000053 0.000085 0.000392 0.001425 0.000035 0.000718
Golgi 0.000680 0.000946 0.000092 0.020189 0.000340 0.000092 0.000157 0.000030 0.000363 0.000320 0.000111 0.000837
Lipid Particles 0.001598 0.001445 0.000025 0.001752 0.000184 0.000015 0.000045 0.000005 0.000280 0.000397 0.000031 0.000075
Mitochondria 0.001334 0.002814 0.001922 0.017793 0.001470 0.000676 0.001937 0.000408 0.001461 0.001248 0.001927 0.001103
Mitotic Spindle 0.000973 0.015168 0.000354 0.025544 0.001139 0.000547 0.001009 0.000984 0.001128 0.004459 0.000550 0.001966
None 0.001934 0.001173 0.000658 0.003059 0.007367 0.001083 0.000866 0.002552 0.006997 0.001107 0.000721 0.002572
Nuclear Periphery 0.000046 0.000440 0.000078 0.001547 0.000340 0.000041 0.000110 0.000233 0.000320 0.000147 0.000088 0.000286
Nuclear Periphery Foci 0.000352 0.001663 0.000021 0.001505 0.000207 0.000044 0.000037 0.000004 0.000217 0.000477 0.000026 0.000064
Nucleolus 0.050618 0.049479 0.042538 0.102109 0.017009 0.078687 0.085718 0.000810 0.019295 0.070872 0.055477 0.004862
Nucleus 0.928933* 0.813539* 0.927462* 0.539784* 0.944871* 0.905558* 0.888102* 0.960008* 0.943787* 0.880937* 0.915667* 0.943199*
Peroxisomes 0.001243 0.003626 0.003502 0.020926 0.000757 0.001678 0.006625 0.000006 0.000790 0.002199 0.004438 0.000842
Vacuole 0.000172 0.015564 0.000460 0.012140 0.000542 0.000398 0.000589 0.000594 0.000517 0.004456 0.000499 0.001056
Vacuole Periphery 0.000163 0.000556 0.000163 0.006400 0.000132 0.000161 0.000189 0.000048 0.000134 0.000267 0.000171 0.000302

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.18 0.77 -0.09 -0.44 -1.62 -3.72 -12.30 5.89 10.18 14.67* -1.54 -3.80 1.26 2.56 12.00
Bud Neck -4.35 -9.44 -3.75 -2.42 -2.29 2.84 -1.19 -6.73 -7.81 -5.97 -0.26 -6.57 -7.33 -6.92 -4.03
Bud Site -2.36 -0.88 -1.94 -1.81 -1.91 1.47 -0.22 1.35 -0.52 1.92 -0.79 -0.99 -1.40 -1.02 -1.12
Cell Periphery -1.22 0.84 -1.18 -1.09 -1.21 4.08 4.75 -0.98 -1.32 -1.31 2.62 4.83 -1.11 -1.35 -1.44
Cytoplasm -3.63 0.89 -1.24 -0.17 -1.24 6.73 6.79 2.54 -4.08 -4.16 -0.76 6.77 0.92 1.28 -3.36
Cytoplasmic Foci -2.86 1.24 -1.34 0.11 -1.47 0.76 4.07 4.31 1.04 5.84 -2.21 4.10 1.77 2.80 -1.12
Eisosomes -1.67 -6.89 -3.57 -2.93 -0.31 -7.52 -13.54 -1.33 1.53 7.16 -8.06 -18.95 -1.67 1.17 7.05
Endoplasmic Reticulum -1.19 1.70 -0.81 0.02 -1.51 9.21 6.95 9.24 -1.57 1.74 3.63 11.73 8.05 0.81 -2.50
Endosome -2.74 1.28 -1.50 -0.80 -1.53 0.98 3.53 3.39 1.09 -1.13 -2.25 3.86 -0.32 1.80 -1.61
Golgi -0.26 0.98 -1.13 -1.11 -1.19 1.33 0.77 1.67 1.72 7.86 0.33 1.25 -0.36 -0.54 -0.92
Lipid Particles 0.10 1.01 0.14 0.06 -1.49 5.84 4.37 6.19 2.03 6.55 -0.37 2.39 2.12 1.59 -0.47
Mitochondria -1.06 -0.96 -1.49 -1.30 -1.40 1.62 -1.31 2.11 2.27 11.31 0.42 -1.32 0.94 0.65 3.40
Mitotic Spindle -1.98 0.71 -1.75 -0.64 -1.83 1.19 -0.14 0.31 -0.81 0.42 -1.57 0.70 -0.67 1.17 -1.28
None 1.17 1.90 -1.08 -1.79 -2.14 5.73 5.84 4.34 -3.87 -4.23 5.77 6.06 4.25 -4.12 -5.19
Nuclear Periphery -2.38 -2.87 -1.14 -0.79 -1.10 7.56 4.37 1.95 -7.68 -3.09 3.02 5.46 0.41 -2.16 -3.41
Nuclear Periphery Foci -0.79 1.04 -0.38 0.34 -1.26 3.99 3.92 4.97 2.06 3.45 -0.69 3.83 3.17 1.75 -0.43
Nucleolus 0.21 0.08 -1.36 -1.42 -1.39 -12.37 -8.68 15.96 15.97 10.71 -12.52 -10.76 6.28 14.82 13.23
Nucleus 4.53 0.66 3.86 2.30 3.78 5.64 6.51 -3.46 -7.94 -8.09 7.59 6.71 -0.56 -7.08 -5.72
Peroxisomes -0.79 -2.75 -1.80 -1.50 -1.49 -1.17 -6.29 2.21 2.95 7.63 -1.50 -7.33 0.59 1.89 8.22
Vacuole -3.07 -3.97 -1.57 1.17 -1.50 0.77 -0.78 0.71 -0.37 1.61 -2.85 -0.36 -0.92 2.67 -0.82
Vacuole Periphery -2.93 -0.39 -1.57 -1.43 -1.56 -0.74 -3.92 5.26 3.24 11.11 -2.89 -3.40 -0.78 0.35 -0.28
Standard name
Human Ortholog
Description Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ies1

Ies1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ies1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available