Standard name
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0.08 0.16 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.05 0.08 0
Bud 0 0 0 0.05 0.06 0.05 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.09 0.15 0.12 0.2 0.1 0 0 0 0 0 0 0 0.2 0.09 0 0 0 0 0 0 0 0.05 0.06 0.05
Cytoplasm 0.64 0.63 0.65 0.5 0.75 0.88 0.91 0.93 0.93 0.9 0.9 0.88 0.69 0.87 0.99 0.93 0.95 0.91 0.09 0.12 0.06 0.14 0.21 0.27
Endoplasmic Reticulum 0.23 0.31 0.26 0.36 0.15 0.06 0 0 0 0 0 0 0.23 0.09 0 0 0.06 0 0.73 0.65 0.7 0.56 0.44 0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.07 0.11 0.16 0.18 0.16 0 0 0 0 0 0 0 0 0 0.09 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0.05 0.05 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 14 36 38 25 14 8 2 5 1 0 2 3 3 1 5 2 5 18 17 36 9 20 9
Bud 7 2 8 11 16 22 25 20 25 28 12 19 1 1 0 4 1 2 4 4 4 2 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 6 4 0 11 9 10 2 6 8
Bud Site 0 0 0 0 0 2 0 1 0 1 0 1 0 0 0 0 0 0
Cell Periphery 33 36 52 47 27 21 12 7 4 2 6 7 28 27 12 9 13 15 4 9 22 8 15 14
Cytoplasm 234 153 294 118 208 428 518 725 409 453 334 424 94 251 333 779 509 398 38 34 28 26 52 85
Endoplasmic Reticulum 83 76 116 84 41 31 14 3 3 0 3 5 31 27 3 31 32 17 293 191 358 107 111 126
Endosome 5 0 5 1 7 3 3 7 2 3 2 1 1 0 0 8 4 1 0 5 1 3 1 2
Golgi 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 7 4 3 1 1 1 1
Mitochondria 3 1 2 2 2 21 30 54 48 79 66 79 0 0 0 26 7 5 5 4 17 16 13 16
Nucleus 0 0 0 0 1 0 1 4 0 1 3 0 0 1 0 7 3 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 5 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
SpindlePole 2 0 4 1 0 1 2 3 2 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 26 0 7 4 9 9 16 11 6 13 4 12 0 1 1 43 19 19 19 12 23 9 19 41
Unique Cell Count 363 244 451 234 278 485 567 779 441 504 373 481 137 287 337 838 536 436 404 296 512 191 250 314
Labelled Cell Count 416 283 524 309 337 552 630 842 504 583 431 553 158 311 350 921 595 472 404 296 512 191 250 314


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 8.6 7.0 8.4 6.9 5.1 4.5 4.4 4.0 3.7 3.8 3.8 8.3 6.7 6.1 5.7 6.2 7.2 8.3 7.9 7.9
Std Deviation (1e-4) 2.7 2.4 1.9 2.1 1.9 1.0 1.1 1.1 0.7 0.9 0.9 0.6 1.8 1.2 1.0 1.4 1.5 2.1 2.2 1.9 2.0
Intensity Change (Log2) 0.26 -0.03 -0.46 -0.66 -0.66 -0.82 -0.92 -0.89 -0.88 0.24 -0.08 -0.21 -0.29 -0.17 0.04 0.24 0.16 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.3 0.5 -3.5 0 0 0 0 0 0 -2.4 -4.1 -5.0 -7.2 -6.2 -4.8
Bud 2.2 2.9 2.4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.0 -0.8 -4.1 0 0 0 0 0 0 2.7 -0.9 -4.1 -8.4 -5.7 -4.6
Cytoplasm -3.7 2.7 8.4 10.3 12.5 10.1 9.2 8.2 8.3 0.7 6.7 11.6 12.7 12.0 9.4
Endoplasmic Reticulum 2.8 -3.5 -8.1 0 0 0 0 0 0 -0.7 -5.5 -9.6 -11.9 -8.7 -9.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 0 0 0 0 0 0 0 0 0 3.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.5 0.4 1.4 -0.2 -0.2 1.1 0 1.0 0 0 0 3.2 1.9 2.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vtc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available