Standard name | |
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Human Ortholog | |
Description | Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 100 | 54 | 48 | 40 | 21 | 37 | 17 | 14 | 15 | – | 9 | 6 | 59 | 62 | 36 | 25 | 41 | 69 | 76 | 48 | 42 | 32 | 67 | 72 |
Bud | 51 | 30 | 44 | 62 | 104 | 80 | 97 | 82 | 53 | – | 57 | 65 | 34 | 50 | 70 | 13 | 29 | 33 | 96 | 88 | 48 | 43 | 69 | 52 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 15 | 21 | 14 | 14 | 15 | 13 |
Bud Site | 8 | 7 | 15 | 21 | 19 | 24 | 25 | 32 | 10 | – | 5 | 14 | 10 | 8 | 4 | 2 | 1 | 4 | – | – | – | – | – | – |
Cell Periphery | 20 | 7 | 9 | 16 | 7 | 6 | 17 | 13 | 11 | – | 23 | 23 | 17 | 24 | 25 | 3 | 6 | 32 | 4 | 1 | 2 | 1 | 4 | 9 |
Cytoplasm | 64 | 71 | 59 | 105 | 94 | 142 | 243 | 290 | 141 | – | 106 | 140 | 63 | 138 | 192 | 56 | 92 | 142 | 32 | 42 | 21 | 17 | 24 | 20 |
Endoplasmic Reticulum | 28 | 27 | 13 | 9 | 8 | 28 | 18 | 15 | 19 | – | 7 | 12 | 13 | 15 | 6 | 7 | 12 | 13 | 30 | 10 | 12 | 11 | 27 | 35 |
Endosome | 9 | 3 | 1 | 1 | 26 | 4 | 20 | 36 | 1 | – | 1 | 1 | 3 | 5 | 3 | 9 | 5 | 13 | 11 | 10 | 7 | 9 | 14 | 7 |
Golgi | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 3 | 0 | 2 | 10 | 28 | 13 | 5 | 1 | 1 | 5 | 9 |
Mitochondria | 33 | 50 | 63 | 137 | 155 | 269 | 363 | 428 | 311 | – | 307 | 420 | 22 | 22 | 81 | 12 | 10 | 33 | 32 | 35 | 43 | 26 | 45 | 49 |
Nucleus | 0 | 8 | 0 | 0 | 10 | 7 | 6 | 15 | 9 | – | 6 | 8 | 3 | 2 | 0 | 1 | 2 | 7 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 1 | 0 | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 3 | 1 | 3 | 7 | 1 | 9 | 10 | 3 | – | 0 | 5 | 1 | 0 | 2 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4 | 6 | 5 | 7 | 24 | 6 | 15 | 16 | 6 | – | 2 | 7 | 7 | 5 | 6 | 1 | 6 | 6 | 0 | 0 | 1 | 0 | 1 | 3 |
SpindlePole | 3 | 0 | 3 | 1 | 12 | 2 | 22 | 29 | 1 | – | 1 | 5 | 0 | 2 | 0 | 1 | 2 | 13 | 1 | 4 | 4 | 2 | 3 | 0 |
Vac/Vac Membrane | 11 | 8 | 4 | 3 | 13 | 17 | 35 | 50 | 10 | – | 10 | 11 | 2 | 2 | 2 | 9 | 14 | 32 | 5 | 31 | 10 | 10 | 15 | 8 |
Unique Cell Count | 245 | 212 | 185 | 274 | 364 | 459 | 646 | 766 | 423 | 392 | 530 | 171 | 260 | 310 | 107 | 177 | 333 | 324 | 309 | 213 | 174 | 300 | 285 | |
Labelled Cell Count | 342 | 278 | 265 | 405 | 503 | 623 | 891 | 1032 | 590 | 537 | 717 | 236 | 338 | 427 | 141 | 232 | 430 | 324 | 309 | 213 | 174 | 300 | 285 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 7.5 | 5.4 | 5.3 | 5.2 | 4.8 | 4.5 | 4.7 | 4.1 | – | 3.9 | 3.6 | 7.7 | 7.7 | 6.6 | 8.6 | 9.6 | 10.2 | 7.5 | 7.1 | 8.2 |
Std Deviation (1e-4) | 1.9 | 1.5 | 0.9 | 1.1 | 1.3 | 0.9 | 1.0 | 1.4 | 1.3 | – | 0.8 | 1.1 | 1.5 | 1.8 | 1.6 | 2.1 | 2.3 | 2.4 | 1.2 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.04 | -0.17 | -0.26 | -0.19 | -0.37 | – | -0.47 | -0.57 | 0.52 | 0.51 | 0.3 | 0.69 | 0.84 | 0.93 | 0.48 | 0.41 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.0 | -6.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.8 | -0.5 | -4.1 | -0.5 | -0.6 | -1.4 |
Bud | -0.3 | 1.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.9 | -1.2 | -0.3 | -2.4 | -1.8 | -4.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -0.2 | -1.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.8 | -2.4 | -3.8 | -2.2 | -3.5 | -4.0 |
Cell Periphery | 0.5 | -1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.8 | 1.7 | 1.4 | 0 | -0.7 | 1.9 |
Cytoplasm | 1.4 | -1.5 | -0.2 | 1.4 | 1.5 | 0.3 | – | -1.2 | -1.4 | 1.0 | 4.4 | 6.5 | 3.4 | 3.9 | 2.4 |
Endoplasmic Reticulum | -1.8 | -2.8 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.2 | -0.5 | -2.9 | -0.2 | -0.1 | -1.6 |
Endosome | 0 | 3.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 |
Mitochondria | 3.4 | 1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -4.7 | -6.8 | -1.9 | -4.3 | -6.7 | -6.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.7 | 0 | 0 | 0 | 0.4 | 0 |
SpindlePole | 0 | 1.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 |
Vacuole | 0 | 0.9 | 1.0 | 1.8 | 2.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8468 | 5.3492 | 5.016 | 4.6237 | 4.7284 | 5.3108 | 5.0765 | 4.9959 | 5.0275 | 5.1842 | 4.791 | 5.1915 | 2.3857 | 2.4533 | 2.8398 | 2.9148 | 3.2668 | 2.7155 |
Actin | 0.1218 | 0.0103 | 0.0284 | 0.0184 | 0.0703 | 0.0637 | 0.1147 | 0.0261 | 0.036 | 0.0365 | 0.1407 | 0.0684 | 0.0516 | 0.0478 | 0.0414 | 0.0338 | 0.0977 | 0.0481 |
Bud | 0.0577 | 0.0404 | 0.3146 | 0.1643 | 0.1252 | 0.0153 | 0.046 | 0.0514 | 0.3451 | 0.209 | 0.159 | 0.0214 | 0.0255 | 0.0644 | 0.2622 | 0.1105 | 0.1572 | 0.0245 |
Bud Neck | 0.0203 | 0.0221 | 0.0086 | 0.026 | 0.0266 | 0.4519 | 0.066 | 0.0099 | 0.0043 | 0.0515 | 0.054 | 0.4572 | 0.026 | 0.0053 | 0.0218 | 0.0038 | 0.0012 | 0.2739 |
Bud Periphery | 0.1332 | 0.0571 | 0.4654 | 0.5973 | 0.4256 | 0.0503 | 0.05 | 0.0351 | 0.3732 | 0.5385 | 0.3193 | 0.0436 | 0.0879 | 0.0496 | 0.3909 | 0.421 | 0.4123 | 0.0899 |
Bud Site | 0.1485 | 0.5272 | 0.1154 | 0.0254 | 0.0543 | 0.037 | 0.1465 | 0.4675 | 0.0708 | 0.0237 | 0.0135 | 0.0202 | 0.1087 | 0.389 | 0.1235 | 0.0192 | 0.0112 | 0.017 |
Cell Periphery | 0.0234 | 0.0086 | 0.0041 | 0.0061 | 0.0045 | 0.0183 | 0.0053 | 0.005 | 0.0027 | 0.003 | 0.0021 | 0.0026 | 0.0135 | 0.0047 | 0.004 | 0.0026 | 0.0023 | 0.0114 |
Cytoplasm | 0.1857 | 0.2314 | 0.0347 | 0.0639 | 0.0846 | 0.1643 | 0.2317 | 0.3327 | 0.095 | 0.0604 | 0.1306 | 0.1923 | 0.4084 | 0.2663 | 0.0643 | 0.2323 | 0.106 | 0.3403 |
Cytoplasmic Foci | 0.0522 | 0.0256 | 0.0025 | 0.0119 | 0.04 | 0.0503 | 0.0717 | 0.0144 | 0.0112 | 0.004 | 0.0322 | 0.0512 | 0.0512 | 0.0404 | 0.0124 | 0.0268 | 0.0967 | 0.0521 |
Eisosomes | 0.0019 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0009 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0373 | 0.0017 | 0.001 | 0.0027 | 0.0028 | 0.0077 | 0.02 | 0.0055 | 0.0016 | 0.0011 | 0.0118 | 0.0043 | 0.0287 | 0.0032 | 0.0013 | 0.0036 | 0.0021 | 0.0088 |
Endosome | 0.053 | 0.0164 | 0.0027 | 0.0128 | 0.0436 | 0.0472 | 0.0576 | 0.0108 | 0.0094 | 0.004 | 0.0233 | 0.0387 | 0.0213 | 0.0204 | 0.0264 | 0.0452 | 0.0038 | 0.0257 |
Golgi | 0.0374 | 0.0106 | 0.0017 | 0.0069 | 0.0199 | 0.0326 | 0.0571 | 0.0041 | 0.0102 | 0.0031 | 0.0262 | 0.0359 | 0.0182 | 0.009 | 0.0219 | 0.0123 | 0.0031 | 0.016 |
Lipid Particles | 0.022 | 0.0007 | 0.0018 | 0.011 | 0.0194 | 0.0026 | 0.0171 | 0.0006 | 0.007 | 0.0008 | 0.018 | 0.0086 | 0.014 | 0.0023 | 0.0016 | 0.0019 | 0.0015 | 0.0019 |
Mitochondria | 0.0333 | 0.0041 | 0.002 | 0.0118 | 0.0154 | 0.0226 | 0.0245 | 0.0022 | 0.0107 | 0.0425 | 0.0295 | 0.013 | 0.0031 | 0.0119 | 0.016 | 0.022 | 0.0036 | 0.0129 |
None | 0.013 | 0.0179 | 0.004 | 0.0035 | 0.0073 | 0.0024 | 0.0309 | 0.0225 | 0.0073 | 0.0014 | 0.0032 | 0.0242 | 0.1248 | 0.0685 | 0.0022 | 0.0442 | 0.0939 | 0.0657 |
Nuclear Periphery | 0.0052 | 0.0008 | 0.0005 | 0.0015 | 0.0022 | 0.0006 | 0.0051 | 0.0009 | 0.0007 | 0.001 | 0.0051 | 0.0006 | 0.0019 | 0.0011 | 0.0004 | 0.0014 | 0.0004 | 0.0007 |
Nucleolus | 0.0021 | 0.0019 | 0.0002 | 0.0008 | 0.0067 | 0.0006 | 0.0016 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0004 |
Nucleus | 0.0042 | 0.0046 | 0.0011 | 0.0021 | 0.0052 | 0.0016 | 0.0034 | 0.0017 | 0.0016 | 0.0014 | 0.0017 | 0.0012 | 0.0013 | 0.0022 | 0.0008 | 0.0018 | 0.0005 | 0.0009 |
Peroxisomes | 0.014 | 0.0015 | 0.0031 | 0.0076 | 0.0075 | 0.0086 | 0.0177 | 0.0008 | 0.0058 | 0.0007 | 0.01 | 0.0072 | 0.0033 | 0.0019 | 0.0026 | 0.0019 | 0.0041 | 0.0041 |
Punctate Nuclear | 0.0053 | 0.0013 | 0.0002 | 0.0008 | 0.0039 | 0.0006 | 0.0178 | 0.0007 | 0.0007 | 0.0002 | 0.0008 | 0.0015 | 0.001 | 0.0013 | 0.0002 | 0.0004 | 0.0006 | 0.0006 |
Vacuole | 0.0242 | 0.0144 | 0.0068 | 0.021 | 0.0293 | 0.019 | 0.0123 | 0.0067 | 0.005 | 0.0133 | 0.012 | 0.0055 | 0.0076 | 0.008 | 0.0052 | 0.0117 | 0.0012 | 0.0037 |
Vacuole Periphery | 0.0042 | 0.0012 | 0.0009 | 0.0042 | 0.0053 | 0.0022 | 0.0026 | 0.0007 | 0.0012 | 0.0031 | 0.0067 | 0.0018 | 0.0008 | 0.0012 | 0.0008 | 0.0028 | 0.0003 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.6058 | 29.9308 | 42.5967 | 39.9465 | 40.8191 | 26.7208 | 37.6029 | 44.1282 | 34.2137 | 44.7939 |
Translational Efficiency | 0.3935 | 0.4127 | 0.3371 | 0.2799 | 0.2394 | 0.4558 | 0.3217 | 0.2204 | 0.2635 | 0.2372 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
845 | 1360 | 1558 | 540 | 1291 | 1956 | 1689 | 70 | 2136 | 3316 | 3247 | 610 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 776.33 | 938.84 | 1157.30 | 996.50 | 804.18 | 879.47 | 1076.39 | 1024.92 | 793.16 | 903.82 | 1115.21 | 999.76 |
Standard Deviation | 141.28 | 153.89 | 169.73 | 146.46 | 125.69 | 134.30 | 172.87 | 175.97 | 132.78 | 145.62 | 176.07 | 150.42 |
Intensity Change Log 2 | — | 0.274209 | 0.576021 | 0.360200 | — | 0.129116 | 0.420611 | 0.349921 | — | 0.202208 | 0.499039 | 0.354979 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006717 | 0.005706 | 0.014447 | 0.003938 | 0.003674 | 0.004794 | 0.005439 | 0.013142 | 0.004878 | 0.005168 | 0.009761 | 0.004995 |
Bud Neck | 0.047246 | 0.015807 | 0.020239 | 0.010551 | 0.031986 | 0.024886 | 0.021091 | 0.020977 | 0.038023 | 0.021163 | 0.020682 | 0.011748 |
Bud Site | 0.454420* | 0.447820* | 0.347351* | 0.397359* | 0.439903* | 0.454787* | 0.479804* | 0.404993* | 0.445646* | 0.451930* | 0.416250* | 0.398235* |
Cell Periphery | 0.005472 | 0.001525 | 0.000701 | 0.000363 | 0.003258 | 0.001363 | 0.000375 | 0.000866 | 0.004134 | 0.001429 | 0.000531 | 0.000421 |
Cytoplasm | 0.185885* | 0.282155* | 0.395589* | 0.384283* | 0.260623* | 0.294939* | 0.353701* | 0.347958* | 0.231057* | 0.289696* | 0.373800* | 0.380114* |
Cytoplasmic Foci | 0.112267 | 0.112957 | 0.075934 | 0.083786 | 0.121432 | 0.092889 | 0.041674 | 0.106397 | 0.117806 | 0.101119 | 0.058113 | 0.086381 |
Eisosomes | 0.000647 | 0.000155 | 0.000082 | 0.000033 | 0.000327 | 0.000280 | 0.000039 | 0.000080 | 0.000454 | 0.000229 | 0.000059 | 0.000039 |
Endoplasmic Reticulum | 0.008253 | 0.006359 | 0.004969 | 0.001008 | 0.005528 | 0.002639 | 0.001148 | 0.000590 | 0.006606 | 0.004165 | 0.002982 | 0.000960 |
Endosome | 0.028133 | 0.024291 | 0.011424 | 0.014340 | 0.024347 | 0.021764 | 0.007710 | 0.004880 | 0.025845 | 0.022800 | 0.009492 | 0.013254 |
Golgi | 0.039047 | 0.032753 | 0.005602 | 0.005257 | 0.030827 | 0.030779 | 0.003076 | 0.002418 | 0.034079 | 0.031588 | 0.004288 | 0.004931 |
Lipid Particles | 0.003277 | 0.001110 | 0.004080 | 0.000987 | 0.003008 | 0.000894 | 0.001215 | 0.001213 | 0.003114 | 0.000983 | 0.002590 | 0.001013 |
Mitochondria | 0.047108 | 0.018091 | 0.000835 | 0.001560 | 0.031368 | 0.027204 | 0.001110 | 0.000083 | 0.037595 | 0.023466 | 0.000978 | 0.001391 |
Mitotic Spindle | 0.003404 | 0.004733 | 0.020054 | 0.009313 | 0.001447 | 0.002952 | 0.015671 | 0.002359 | 0.002221 | 0.003683 | 0.017774 | 0.008515 |
None | 0.004472 | 0.001228 | 0.000388 | 0.000740 | 0.000747 | 0.000250 | 0.000261 | 0.000054 | 0.002221 | 0.000651 | 0.000322 | 0.000661 |
Nuclear Periphery | 0.000138 | 0.000024 | 0.000248 | 0.000074 | 0.000040 | 0.000023 | 0.000096 | 0.000036 | 0.000079 | 0.000024 | 0.000169 | 0.000069 |
Nuclear Periphery Foci | 0.000873 | 0.000159 | 0.001992 | 0.000565 | 0.000207 | 0.000074 | 0.000718 | 0.001134 | 0.000471 | 0.000108 | 0.001329 | 0.000630 |
Nucleolus | 0.000526 | 0.000113 | 0.000103 | 0.000059 | 0.000338 | 0.000066 | 0.000224 | 0.000046 | 0.000412 | 0.000085 | 0.000166 | 0.000057 |
Nucleus | 0.006688 | 0.002542 | 0.019378 | 0.002476 | 0.002225 | 0.001139 | 0.005717 | 0.004420 | 0.003991 | 0.001714 | 0.012272 | 0.002699 |
Peroxisomes | 0.006787 | 0.004260 | 0.005012 | 0.006927 | 0.003774 | 0.004302 | 0.006073 | 0.013258 | 0.004966 | 0.004285 | 0.005564 | 0.007654 |
Vacuole | 0.019558 | 0.023583 | 0.070083 | 0.072073 | 0.020009 | 0.022235 | 0.053389 | 0.074742 | 0.019831 | 0.022788 | 0.061399 | 0.072379 |
Vacuole Periphery | 0.019082 | 0.014629 | 0.001490 | 0.004307 | 0.014933 | 0.011741 | 0.001469 | 0.000355 | 0.016574 | 0.012925 | 0.001479 | 0.003853 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.65 | -3.54 | 2.02 | 1.70 | 6.18 | -1.35 | -1.67 | -1.74 | -1.54 | -1.50 | -0.37 | -4.39 | 0.12 | 0.38 | 3.42 |
Bud Neck | 8.00 | 7.27 | 9.74 | 4.04 | 5.63 | 2.70 | 5.13 | 2.19 | 1.04 | 0.07 | 7.57 | 8.82 | 12.21 | 7.30 | 6.08 |
Bud Site | 0.45 | 10.83 | 4.76 | 4.81 | -3.78 | -1.22 | 1.21 | 1.85 | 2.21 | 1.48 | -0.66 | 8.49 | 5.17 | 5.80 | 0.04 |
Cell Periphery | 6.46 | 8.09 | 8.61 | 7.59 | 4.95 | 3.40 | 5.31 | 3.90 | 1.66 | -1.53 | 6.51 | 8.94 | 9.12 | 9.81 | 1.68 |
Cytoplasm | -9.14 | -19.70 | -13.84 | -7.52 | 0.25 | -3.67 | -10.76 | -2.75 | -1.75 | 0.58 | -8.28 | -20.29 | -11.96 | -7.70 | -0.34 |
Cytoplasmic Foci | -0.09 | 6.25 | 3.24 | 3.63 | -1.85 | 4.96 | 14.83 | 0.45 | -1.08 | -3.80 | 3.75 | 14.81 | 4.25 | 1.93 | -5.10 |
Eisosomes | 4.78 | 5.57 | 6.05 | 7.46 | 8.14 | 1.12 | 11.16 | 6.45 | 4.54 | -1.59 | 4.69 | 9.12 | 9.57 | 8.99 | 4.17 |
Endoplasmic Reticulum | 1.08 | 2.16 | 5.03 | 4.89 | 6.09 | 3.09 | 4.85 | 5.52 | 4.26 | 1.53 | 2.71 | 4.39 | 6.94 | 5.57 | 4.80 |
Endosome | 1.66 | 8.53 | 5.60 | 5.01 | -1.49 | 1.43 | 10.66 | 10.42 | 10.75 | 1.86 | 2.14 | 13.44 | 6.82 | 5.84 | -2.14 |
Golgi | 1.79 | 11.70 | 11.21 | 12.96 | -0.06 | 0.02 | 14.03 | 14.03 | 15.55 | 0.77 | 1.18 | 18.12 | 15.93 | 17.44 | -0.81 |
Lipid Particles | 4.42 | -2.36 | 4.46 | 0.13 | 9.59 | 3.70 | 2.50 | 0.87 | -1.79 | -0.95 | 5.38 | 0.36 | 4.45 | -1.02 | 6.36 |
Mitochondria | 6.69 | 11.50 | 11.26 | 9.87 | -1.08 | 1.50 | 13.86 | 14.35 | 15.60 | 4.77 | 5.87 | 17.67 | 17.09 | 16.96 | -0.65 |
Mitotic Spindle | -1.06 | -8.63 | -2.43 | -1.94 | 3.78 | -2.89 | -8.58 | -1.19 | 0.37 | 7.03 | -2.47 | -12.46 | -3.03 | -2.36 | 3.88 |
None | 2.22 | 3.15 | 2.80 | 0.66 | -0.57 | 3.17 | 2.99 | 4.51 | 4.56 | 6.81 | 2.66 | 3.61 | 2.51 | 0.08 | -0.66 |
Nuclear Periphery | 2.49 | -5.00 | 0.94 | -2.49 | 7.60 | 1.45 | -6.07 | -1.03 | -2.32 | 4.41 | 2.85 | -7.54 | -0.22 | -2.69 | 6.64 |
Nuclear Periphery Foci | 1.57 | -3.56 | 0.55 | -3.81 | 8.04 | 1.99 | -5.08 | -1.65 | -1.75 | -1.13 | 1.97 | -5.80 | -1.37 | -3.77 | 4.05 |
Nucleolus | 3.34 | 3.19 | 3.74 | 1.92 | 4.24 | 1.89 | 0.28 | 2.04 | 0.74 | 1.72 | 3.28 | 1.64 | 3.50 | 1.02 | 2.03 |
Nucleus | 1.98 | -9.20 | 1.79 | -0.78 | 15.30 | 2.83 | -8.54 | -2.62 | -3.61 | 3.23 | 2.62 | -12.80 | 0.53 | -3.98 | 16.25 |
Peroxisomes | 3.57 | 2.98 | 0.29 | -2.36 | -1.98 | -1.38 | -3.08 | -2.19 | -2.04 | -1.70 | 1.87 | -0.21 | -2.30 | -3.10 | -2.16 |
Vacuole | -1.77 | -19.50 | -10.17 | -9.58 | 1.81 | -1.12 | -14.96 | -4.27 | -4.16 | -1.06 | -1.99 | -24.26 | -11.08 | -10.67 | 0.05 |
Vacuole Periphery | 1.84 | 8.36 | 6.33 | 6.65 | -2.20 | 2.41 | 12.34 | 13.78 | 14.11 | 2.93 | 2.92 | 14.26 | 9.00 | 7.83 | -2.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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