Standard name
Human Ortholog
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0.05 0 0.1 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.95 0.98 0.91 0.88 0.88 0.74 0.79 0.76 0.72 0.71 0.92 0.95 0.97 0.95 0.95 0.95 0.9 0.98 0.94 0.79 0.86 0.85
Endoplasmic Reticulum 0 0 0.07 0 0.08 0.1 0.1 0.19 0.15 0.11 0.09 0.12 0.06 0 0 0 0.06 0 0 0 0 0.11 0.1 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.11 0.22 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 3 10 13 9 10 11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 2 1 0 0 0 3 0 0 0 0 0 0 0 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 5 6 7 15 13 16 10 13 7 23 7 8 6 4 8 3 0 0 0 0 0 0
Cytoplasm 81 160 177 237 267 297 241 272 253 217 139 159 216 314 360 103 106 81 77 159 182 115 269 250
Endoplasmic Reticulum 3 3 13 6 24 35 27 69 48 32 17 26 15 12 9 3 7 1 1 0 4 15 31 27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 8 2 1 1 2 0 0 0 0 1 0 0 0 0 0 1 0
Mitochondria 1 0 0 0 0 2 1 10 21 31 42 21 0 0 1 1 0 1 0 0 0 2 1 1
Nucleus 0 0 0 0 0 2 1 0 1 4 2 3 0 0 0 0 0 0 3 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 2 1 2 21 0 7 4 9 1 2 0 4 1 2 0 1 5 5 7 8
Unique Cell Count 85 163 187 242 294 336 275 368 321 287 194 223 234 330 372 108 112 85 86 163 194 146 315 295
Labelled Cell Count 85 167 195 250 300 355 290 407 348 314 223 259 239 336 376 115 123 88 86 163 194 146 315 295


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.3 31.8 24.0 22.3 21.2 18.1 16.3 15.3 14.5 12.9 12.3 13.1 30.9 31.4 31.2 33.1 35.2 33.0 27.0 28.0 30.1
Std Deviation (1e-4) 6.9 5.6 4.3 3.7 3.3 2.8 2.9 3.0 2.6 2.4 2.4 2.6 6.5 5.8 5.4 7.4 9.0 7.5 6.0 5.9 7.9
Intensity Change (Log2) – – – -0.11 -0.18 -0.41 -0.56 -0.65 -0.73 -0.89 -0.97 -0.87 0.37 0.39 0.38 0.47 0.55 0.46 0.17 0.22 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.0 1.1 1.0 0.3 1.0 0.5 3.1 0.2 0 0 0 0 0
Cytoplasm 1.8 -1.5 -2.4 -2.5 -5.9 -4.8 -5.4 -6.0 -6.1 -1.0 0.2 1.2 0.3 0 0.2
Endoplasmic Reticulum -2.2 0.5 1.3 1.1 3.7 2.7 1.5 0.7 1.6 -0.2 -1.7 -2.6 -1.5 -0.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 4.6 6.7 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.6883 27.0441 20.0037 21.3908 18.7886 20.3828 22.6926 33.4393 30.3142 25.2982 26.3264 30.2521 22.1459 33.8871 28.5361 23.9028 27.7314 28.0521
Actin 0.0411 0.001 0.0402 0.0118 0.0246 0.0149 0.0224 0.0006 0.0073 0.0011 0.0256 0.0009 0.0191 0.0016 0.0079 0.0111 0.0003 0.0017
Bud 0.0025 0.0008 0.002 0.0014 0.005 0.0018 0.0019 0.0003 0.0008 0.0015 0.0011 0.0014 0.0014 0.0002 0.001 0.0019 0.0003 0.0007
Bud Neck 0.0036 0.0003 0.0017 0.0014 0.0008 0.0011 0.0008 0.0002 0.0005 0.001 0.0027 0.001 0.0007 0.0002 0.0004 0.0008 0.0006 0.0011
Bud Periphery 0.0023 0.0003 0.0042 0.0004 0.0022 0.0016 0.0027 0.0002 0.0003 0.0007 0.0012 0.0011 0.0013 0.0001 0.0005 0.0006 0 0.0004
Bud Site 0.0245 0.0008 0.0097 0.0012 0.0014 0.0008 0.0019 0.0002 0.0004 0.003 0.0022 0.0002 0.0019 0.0001 0.0011 0.0012 0.0001 0.0002
Cell Periphery 0.0019 0.001 0.0025 0.0003 0.0004 0.0006 0.001 0.0004 0.0002 0.0007 0.0004 0.0006 0.0007 0.0001 0.0002 0.0002 0.0001 0.0002
Cytoplasm 0.564 0.9479 0.7284 0.6703 0.5988 0.67 0.7303 0.9569 0.9089 0.6885 0.734 0.8573 0.7515 0.9736 0.9033 0.7913 0.8781 0.8668
Cytoplasmic Foci 0.0387 0.0028 0.0143 0.023 0.038 0.0115 0.0248 0.0034 0.0064 0.0218 0.0278 0.0134 0.0193 0.0023 0.0172 0.0217 0.0108 0.0243
Eisosomes 0.0007 0 0.0014 0 0.0001 0.0003 0.0002 0 0 0.0003 0.0002 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0575 0.0213 0.0326 0.066 0.0532 0.0777 0.0309 0.0155 0.0111 0.0117 0.0271 0.0314 0.052 0.0097 0.0189 0.0043 0.0125 0.0199
Endosome 0.0797 0.0063 0.0213 0.0591 0.0781 0.0622 0.047 0.0085 0.0221 0.0794 0.0883 0.0473 0.048 0.0039 0.0185 0.0213 0.0256 0.0465
Golgi 0.0123 0.0015 0.0067 0.0043 0.0119 0.0114 0.0103 0.0012 0.0052 0.0058 0.0458 0.0109 0.0097 0.0034 0.003 0.0052 0.003 0.007
Lipid Particles 0.0194 0.0004 0.0214 0.0016 0.0338 0.0018 0.0236 0.0007 0.0004 0.0181 0.0057 0.0003 0.0055 0 0.0022 0.0075 0.0494 0.0036
Mitochondria 0.0216 0.0008 0.0391 0.0043 0.023 0.0707 0.0222 0.0004 0.0062 0.0279 0.0207 0.0093 0.0219 0.0008 0.0014 0.0041 0.0017 0.0102
None 0.0111 0.0003 0.0035 0.0004 0.0027 0.0037 0.0024 0.0001 0.0002 0.0086 0.0005 0.0007 0.008 0.0002 0.0011 0.0019 0.0002 0.0029
Nuclear Periphery 0.0266 0.0022 0.0055 0.0049 0.0357 0.037 0.0077 0.0007 0.0011 0.0053 0.0015 0.0015 0.0135 0.0008 0.0017 0.0018 0.0021 0.0011
Nucleolus 0.0027 0 0.0017 0.0001 0.0017 0.0002 0.0011 0 0 0.0011 0.0001 0.0001 0.0008 0 0.0001 0.0002 0 0.0002
Nucleus 0.0125 0.0012 0.0024 0.0022 0.0041 0.0044 0.017 0.0005 0.0007 0.0023 0.0009 0.001 0.0058 0.0005 0.001 0.0024 0.001 0.0009
Peroxisomes 0.0085 0 0.0094 0.0263 0.0033 0.0013 0.0061 0 0.0002 0.0042 0.0014 0.0001 0.0027 0 0.0021 0.0407 0.0009 0.0008
Punctate Nuclear 0.005 0 0.0018 0.0002 0.0273 0.0033 0.0016 0 0 0.0005 0.0003 0.0001 0.0024 0 0.0022 0.0008 0.0001 0.0003
Vacuole 0.0479 0.0096 0.0387 0.0907 0.0341 0.0136 0.0372 0.0082 0.0205 0.0862 0.0087 0.0148 0.0244 0.0021 0.0128 0.0584 0.01 0.007
Vacuole Periphery 0.0159 0.0016 0.0112 0.0301 0.0197 0.0101 0.0071 0.0019 0.0072 0.0302 0.0038 0.0065 0.0092 0.0004 0.0035 0.0226 0.0032 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.0527 95.6248 89.1022 99.4383 86.128 111.7927 95.472 94.9052 86.4029 90.6555
Translational Efficiency 1.0159 1.2746 1.1573 1.0323 1.072 0.9553 1.007 0.896 0.9613 0.9332

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1539 1167 326 1213 1383 1317 147 1847 2922 2484 473 3060

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2044.18 2308.96 3398.76 2950.25 1966.09 2338.84 3257.09 3066.57 2007.22 2324.80 3354.73 3020.46
Standard Deviation 358.90 493.25 545.12 585.51 357.95 420.36 490.42 587.62 360.56 456.30 532.78 589.54
Intensity Change Log 2 — 0.175721 0.733486 0.529315 — 0.250464 0.728254 0.641297 — 0.212849 0.730924 0.585302

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000129 0.000170 0.000348 0.000310 0.000151 0.000226 0.000481 0.000254 0.000139 0.000200 0.000389 0.000276
Bud Neck 0.000154 0.000572 0.006924 0.001996 0.000277 0.000349 0.007105 0.000782 0.000212 0.000454 0.006980 0.001263
Bud Site 0.005452 0.008425 0.011261 0.016483 0.008242 0.005905 0.016308 0.014831 0.006773 0.007089 0.012830 0.015486
Cell Periphery 0.001535 0.003443 0.033793 0.019556 0.002820 0.001503 0.024134 0.025163 0.002143 0.002414 0.030791 0.022941
Cytoplasm 0.851974* 0.805903* 0.371076* 0.562372* 0.792515* 0.887561* 0.466561* 0.790744* 0.823831* 0.849198* 0.400751* 0.700216*
Cytoplasmic Foci 0.025281 0.024144 0.026874 0.007546 0.038547 0.019902 0.028912 0.004407 0.031560 0.021895 0.027508 0.005651
Eisosomes 0.000001 0.000002 0.000009 0.000004 0.000001 0.000002 0.000009 0.000003 0.000001 0.000002 0.000009 0.000003
Endoplasmic Reticulum 0.001729 0.002800 0.000718 0.000447 0.003778 0.001203 0.000711 0.000850 0.002699 0.001953 0.000716 0.000690
Endosome 0.001553 0.003203 0.002900 0.002531 0.003223 0.002157 0.004611 0.000837 0.002343 0.002648 0.003432 0.001508
Golgi 0.043132 0.071436 0.092287 0.021581 0.065820 0.037834 0.090800 0.015961 0.053870 0.053620 0.091825 0.018189
Lipid Particles 0.001788 0.006767 0.029852 0.020992 0.005370 0.002881 0.037712 0.018439 0.003483 0.004707 0.032294 0.019451
Mitochondria 0.000161 0.000328 0.000154 0.000420 0.000162 0.000193 0.000087 0.000337 0.000161 0.000257 0.000133 0.000370
Mitotic Spindle 0.001049 0.003411 0.005779 0.003662 0.001435 0.001216 0.009508 0.000373 0.001232 0.002247 0.006938 0.001677
None 0.000759 0.000780 0.001146 0.000406 0.000400 0.000690 0.000714 0.000526 0.000589 0.000732 0.001012 0.000478
Nuclear Periphery 0.000062 0.000118 0.002201 0.000294 0.000109 0.000048 0.001106 0.000110 0.000084 0.000081 0.001861 0.000183
Nuclear Periphery Foci 0.004350 0.008455 0.027161 0.004602 0.005936 0.003551 0.025428 0.003303 0.005101 0.005855 0.026622 0.003818
Nucleolus 0.000044 0.000029 0.000158 0.000272 0.000034 0.000020 0.000105 0.000118 0.000039 0.000024 0.000142 0.000179
Nucleus 0.001560 0.002393 0.015932 0.005327 0.001424 0.002293 0.017129 0.003306 0.001496 0.002340 0.016304 0.004107
Peroxisomes 0.000030 0.000036 0.000035 0.000199 0.000044 0.000039 0.000051 0.000213 0.000037 0.000038 0.000040 0.000208
Vacuole 0.011014 0.024186 0.274170* 0.293327* 0.018565 0.014556 0.219329* 0.101307 0.014588 0.019080 0.257127* 0.177425
Vacuole Periphery 0.048244 0.033400 0.097222 0.037674 0.051147 0.017871 0.049198 0.018135 0.049618 0.025167 0.082297 0.025880

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.57 -2.90 -1.53 -1.42 0.67 -0.55 -4.66 -2.64 -0.36 3.21 -0.75 -5.02 -2.49 -1.11 2.43
Bud Neck -5.99 -9.47 -12.77 -9.87 6.59 -1.45 -6.14 -8.35 -6.25 5.72 -5.66 -11.26 -15.24 -11.28 9.43
Bud Site -3.22 -3.31 -7.24 -4.97 -1.87 2.53 -3.34 -5.30 -6.64 1.10 -0.50 -4.38 -9.00 -8.15 -0.82
Cell Periphery -5.93 -11.26 -10.19 -8.78 4.95 3.81 -5.69 -13.83 -14.58 -0.43 -1.20 -12.45 -17.28 -16.86 3.22
Cytoplasm 4.68 25.57 25.92 19.71 -9.44 -10.64 11.54 0.53 11.92 -11.43 -3.75 26.85 17.31 20.25 -18.50
Cytoplasmic Foci 0.54 -1.65 10.89 11.04 7.40 8.71 1.29 18.86 12.92 7.33 6.56 -0.00 21.54 17.24 10.53
Eisosomes -4.72 -8.50 -7.51 -5.15 5.45 -0.42 -6.20 -4.55 -1.48 4.41 -1.24 -10.31 -8.01 -2.95 6.94
Endoplasmic Reticulum -1.64 5.07 6.85 3.80 3.15 4.20 5.66 5.36 1.10 -0.90 1.73 7.18 7.17 3.72 0.19
Endosome -5.83 -6.13 -3.46 2.12 1.71 3.39 -2.51 9.74 5.20 7.04 -1.52 -4.72 4.99 5.63 8.19
Golgi -6.33 -8.94 8.78 12.83 14.74 7.44 -2.22 15.99 8.24 8.39 0.07 -7.59 17.79 15.16 17.12
Lipid Particles -7.11 -11.41 -11.99 -7.96 3.64 2.76 -6.53 -8.73 -11.26 3.77 -2.30 -12.54 -14.78 -13.44 5.45
Mitochondria -0.79 -0.16 -1.48 -0.21 -1.47 -0.29 3.09 -2.20 -1.12 -3.05 -0.83 0.65 -2.52 -0.74 -2.79
Mitotic Spindle -2.52 -7.25 -1.93 0.05 2.17 1.11 -2.69 6.67 5.49 3.02 -2.26 -5.63 -0.70 1.06 4.92
None -0.23 -3.65 4.79 4.95 7.66 -1.81 -3.72 -1.58 0.83 1.51 -1.50 -5.78 1.67 2.39 6.20
Nuclear Periphery -4.66 -11.65 -2.34 -1.77 9.33 2.15 -8.13 -0.12 -8.77 8.30 0.19 -13.18 -2.40 -2.59 12.08
Nuclear Periphery Foci -5.78 -13.81 -0.52 5.56 13.72 4.64 -7.07 5.33 0.48 8.08 -1.78 -15.19 3.30 5.30 16.25
Nucleolus 0.78 -5.35 -7.60 -10.58 -4.44 1.02 -4.01 -4.56 -16.63 -0.52 1.26 -7.07 -8.47 -15.41 -2.55
Nucleus -2.65 -9.08 -9.56 -8.48 5.84 -1.62 -5.37 -4.30 -4.65 4.77 -2.82 -10.40 -9.97 -9.55 8.27
Peroxisomes -0.91 -1.25 -6.32 -6.11 -6.01 0.49 -1.30 -8.73 -8.36 -7.96 -0.16 -1.31 -10.84 -10.46 -10.36
Vacuole -6.68 -20.22 -36.61 -34.25 -1.42 2.28 -11.32 -21.65 -22.30 6.58 -3.55 -22.93 -38.94 -37.13 6.99
Vacuole Periphery 3.91 -5.95 3.90 -0.23 8.30 9.83 0.70 10.36 -0.00 4.16 9.64 -5.01 10.65 0.49 10.22
Standard name
Human Ortholog
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Gcn20

Gcn20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcn20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available