Standard name
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0.05 0 0.1 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.95 0.98 0.91 0.88 0.88 0.74 0.79 0.76 0.72 0.71 0.92 0.95 0.97 0.95 0.95 0.95 0.9 0.98 0.94 0.79 0.86 0.85
Endoplasmic Reticulum 0 0 0.07 0 0.08 0.1 0.1 0.19 0.15 0.11 0.09 0.12 0.06 0 0 0 0.06 0 0 0 0 0.11 0.1 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.11 0.22 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 3 10 13 9 10 11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 2 1 0 0 0 3 0 0 0 0 0 0 0 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 5 6 7 15 13 16 10 13 7 23 7 8 6 4 8 3 0 0 0 0 0 0
Cytoplasm 81 160 177 237 267 297 241 272 253 217 139 159 216 314 360 103 106 81 77 159 182 115 269 250
Endoplasmic Reticulum 3 3 13 6 24 35 27 69 48 32 17 26 15 12 9 3 7 1 1 0 4 15 31 27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 8 2 1 1 2 0 0 0 0 1 0 0 0 0 0 1 0
Mitochondria 1 0 0 0 0 2 1 10 21 31 42 21 0 0 1 1 0 1 0 0 0 2 1 1
Nucleus 0 0 0 0 0 2 1 0 1 4 2 3 0 0 0 0 0 0 3 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 2 1 2 21 0 7 4 9 1 2 0 4 1 2 0 1 5 5 7 8
Unique Cell Count 85 163 187 242 294 336 275 368 321 287 194 223 234 330 372 108 112 85 86 163 194 146 315 295
Labelled Cell Count 85 167 195 250 300 355 290 407 348 314 223 259 239 336 376 115 123 88 86 163 194 146 315 295


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.3 31.8 24.0 22.3 21.2 18.1 16.3 15.3 14.5 12.9 12.3 13.1 30.9 31.4 31.2 33.1 35.2 33.0 27.0 28.0 30.1
Std Deviation (1e-4) 6.9 5.6 4.3 3.7 3.3 2.8 2.9 3.0 2.6 2.4 2.4 2.6 6.5 5.8 5.4 7.4 9.0 7.5 6.0 5.9 7.9
Intensity Change (Log2) -0.11 -0.18 -0.41 -0.56 -0.65 -0.73 -0.89 -0.97 -0.87 0.37 0.39 0.38 0.47 0.55 0.46 0.17 0.22 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.0 1.1 1.0 0.3 1.0 0.5 3.1 0.2 0 0 0 0 0
Cytoplasm 1.8 -1.5 -2.4 -2.5 -5.9 -4.8 -5.4 -6.0 -6.1 -1.0 0.2 1.2 0.3 0 0.2
Endoplasmic Reticulum -2.2 0.5 1.3 1.1 3.7 2.7 1.5 0.7 1.6 -0.2 -1.7 -2.6 -1.5 -0.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 4.6 6.7 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0