Standard name
Description Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane and may connect Mic60p with the Mic10p-Mic12p-Mic27p subcomplex; both yeast and human Mic19p become oxidized, and oxidation may regulate MICOS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.31 0.32 0.32 0.21 0.12 0.15 – 0.13 0.15 0.15 0.18 0.34 0.41 0.31 0.27 0.24 0.26 0.16 0.15 0.11 0.19 0.27 0.25
Bud 0 0.05 0 0.09 0.05 0.05 0 – 0 0.08 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0.05 0 0 0 – 0 0 0 0 0.07 0 0 0.06 0.11 0 0 0 0 0 0 0
Cytoplasm 0.11 0.29 0.08 0.25 0.21 0.23 0.19 – 0.18 0.14 0.12 0.13 0.17 0.16 0.13 0.41 0.45 0.38 0 0.12 0 0 0 0
Endoplasmic Reticulum 0.33 0.33 0 0.19 0.2 0.17 0.12 – 0.2 0.14 0.2 0.19 0.36 0.2 0.23 0.07 0.06 0.06 0.19 0.1 0.08 0.24 0.19 0.14
Endosome 0.25 0.05 0.4 0.12 0.16 0.1 0.22 – 0.12 0.2 0.1 0.1 0.07 0.17 0.16 0 0 0.13 0.27 0.21 0.3 0.21 0.19 0.25
Golgi 0.16 0.11 0.2 0.14 0.12 0.07 0.16 – 0.05 0.1 0.09 0.14 0.23 0.19 0.36 0.18 0.1 0.18 0.12 0.08 0.15 0.12 0.15 0.17
Mitochondria 0 0 0 0.05 0 0.24 0.06 – 0.32 0.33 0.4 0.35 0 0 0 0 0.06 0.07 0.08 0.08 0.2 0.07 0.06 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.05 0.16 0.06 0.2 0.23 0.23 – 0.13 0.1 0.11 0.08 0 0.05 0 0.07 0.05 0.06 0 0.14 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 17 8 49 39 21 44 – 25 32 32 49 57 140 86 34 15 27 22 19 7 26 65 75
Bud 2 3 1 14 9 8 4 – 5 16 5 12 5 19 6 6 1 4 2 4 2 2 2 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 5 1 2 6 7
Bud Site 0 0 0 0 0 1 0 – 0 1 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 0 7 7 1 4 – 4 5 6 5 11 15 8 8 7 4 0 0 0 1 1 2
Cytoplasm 11 16 2 39 40 39 55 – 35 29 26 36 28 53 36 52 28 39 5 15 1 5 7 6
Endoplasmic Reticulum 32 18 1 30 38 29 35 – 40 29 44 52 60 69 64 9 4 6 27 12 5 32 44 40
Endosome 24 3 10 19 30 16 64 – 24 42 22 28 12 58 43 5 1 13 37 26 20 28 45 74
Golgi 15 6 5 22 23 11 46 – 10 20 20 37 38 63 99 23 6 19 17 9 10 16 36 50
Mitochondria 3 2 1 8 2 40 18 – 63 68 86 94 1 7 2 2 4 7 11 10 14 8 15 17
Nucleus 0 0 0 2 2 2 2 – 0 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 1 – 2 0 0 0 0 0 1 0 0 0 2 0 0 1 1 1
Nucleolus 2 0 0 1 0 2 2 – 4 5 3 4 2 2 0 0 0 1 0 0 0 0 0 0
Peroxisomes 1 0 1 0 0 0 1 – 0 0 1 0 0 3 4 0 0 0 1 0 1 0 4 7
SpindlePole 1 0 0 7 9 1 13 – 1 7 3 9 2 10 4 2 0 4 1 1 0 0 2 2
Vac/Vac Membrane 16 3 4 10 37 38 66 – 25 21 23 22 4 17 7 9 3 6 5 17 0 4 3 5
Unique Cell Count 96 55 25 155 189 168 289 198 208 215 268 167 338 276 128 62 103 142 126 69 136 241 298
Labelled Cell Count 124 72 33 208 236 210 355 238 275 273 351 220 456 360 152 69 131 142 126 69 136 241 298


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.0 11.9 10.2 10.5 11.1 7.9 9.9 – 8.5 8.7 8.5 8.9 13.3 11.0 13.3 13.6 14.9 13.8 10.9 11.3 11.3
Std Deviation (1e-4) 1.6 1.5 1.3 1.6 1.6 1.7 1.4 – 1.4 1.4 1.4 1.6 1.8 1.9 1.8 2.3 2.8 2.6 1.5 1.8 1.6
Intensity Change (Log2) – – – 0.04 0.13 -0.38 -0.05 – -0.26 -0.23 -0.27 -0.2 0.39 0.11 0.39 0.42 0.55 0.44 0.09 0.14 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.3 0 0 – 0 0 0 0 0.2 0.9 -0.1 -0.6 -0.7 -0.6
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0 1.7 0 – 0 0 0 0 0 0 0 3.1 3.3 2.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 3.2 0 2.2 0 0 0
Endosome 0 0 0 -2.0 – 0 -2.2 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0.3 0 1.6 0 0 0
Mitochondria 0 0 2.3 0 – 2.9 3.0 3.5 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0.7 0.8 – 0 0 0 0 0 0 0 0 0 0