Standard name
Description Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.96 0.93 0.86 0.83 0.76 0.75 0.64 0.65 0.63 0.98 0.98 0.99 0.98 0.97 0.96 0.96 0.92 0.94 0.89 0.85 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.15 0.07 0.3 0.33 0.4 0.52 0.61 0.58 0.53 0 0 0 0 0 0 0 0 0 0 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 0 0 1 0 1 0 0 0 0 3 1 1 0 1 0 0 0 1
Bud 0 7 1 3 2 1 4 6 25 12 20 20 2 2 0 4 0 3 1 5 1 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 2 6 4 6 7 10 7 7 5 12 18 8 8 4 17 17 16 0 0 0 0 0 0
Cytoplasm 416 407 255 273 331 423 535 455 397 313 408 439 148 148 103 650 604 415 407 392 234 128 168 167
Endoplasmic Reticulum 0 2 1 0 3 4 2 1 2 2 0 11 0 1 1 22 21 23 1 1 4 1 4 1
Endosome 0 0 0 0 4 5 4 12 0 2 0 2 0 0 0 3 5 3 0 3 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 1 1 0
Mitochondria 3 7 8 44 26 150 214 239 274 298 364 371 0 0 0 27 15 8 3 5 1 3 10 6
Nucleus 0 0 0 1 0 2 1 1 4 0 0 2 2 2 1 1 2 4 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 1 0 2 3 4 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 2 0 2 2 0 0 0 1 0 0 0 1 0 0 1 1
Vac/Vac Membrane 6 2 0 2 7 10 12 14 8 7 5 9 0 0 0 5 4 15 3 6 0 1 5 6
Unique Cell Count 424 415 257 284 357 492 648 598 532 488 629 697 151 151 104 663 624 433 424 426 248 144 198 193
Labelled Cell Count 433 427 272 327 380 605 785 739 724 642 813 880 160 161 109 734 670 488 424 426 248 144 198 193


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.3 6.3 6.3 5.9 4.9 4.4 4.4 4.2 3.6 3.8 3.8 6.2 6.2 6.1 6.4 7.0 8.1 7.0 6.7 7.4
Std Deviation (1e-4) 1.3 1.0 1.7 1.7 1.7 1.8 1.4 1.4 1.3 1.1 1.4 0.9 0.8 0.8 0.8 1.3 1.4 2.0 1.5 1.9 2.1
Intensity Change (Log2) – – – -0.01 -0.09 -0.36 -0.53 -0.53 -0.58 -0.83 -0.73 -0.75 -0.03 -0.03 -0.05 0.01 0.14 0.37 0.14 0.09 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.6 0 0 0 0 0 0 0 1.6 1.6 0 0.2 0.3 1.0
Cytoplasm -2.3 -3.8 -5.9 -6.8 -8.3 -8.5 -10.7 -10.7 -11.2 -1.1 -1.1 -0.2 -1.3 -2.1 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.6 2.6 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.9 2.2 8.7 0 0 0 0 0 0 0 0 0 0.7 -0.6 -1.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available