Standard name | |
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Description | Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 7 | 1 | 3 | 2 | 1 | 4 | 6 | 25 | 12 | 20 | 20 | 2 | 2 | 0 | 4 | 0 | 3 | 1 | 5 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 2 | 6 | 4 | 6 | 7 | 10 | 7 | 7 | 5 | 12 | 18 | 8 | 8 | 4 | 17 | 17 | 16 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 416 | 407 | 255 | 273 | 331 | 423 | 535 | 455 | 397 | 313 | 408 | 439 | 148 | 148 | 103 | 650 | 604 | 415 | 407 | 392 | 234 | 128 | 168 | 167 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 3 | 4 | 2 | 1 | 2 | 2 | 0 | 11 | 0 | 1 | 1 | 22 | 21 | 23 | 1 | 1 | 4 | 1 | 4 | 1 |
Endosome | 0 | 0 | 0 | 0 | 4 | 5 | 4 | 12 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 3 | 5 | 3 | 0 | 3 | 0 | 1 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 |
Mitochondria | 3 | 7 | 8 | 44 | 26 | 150 | 214 | 239 | 274 | 298 | 364 | 371 | 0 | 0 | 0 | 27 | 15 | 8 | 3 | 5 | 1 | 3 | 10 | 6 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 4 | 0 | 0 | 2 | 2 | 2 | 1 | 1 | 2 | 4 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 4 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 6 | 2 | 0 | 2 | 7 | 10 | 12 | 14 | 8 | 7 | 5 | 9 | 0 | 0 | 0 | 5 | 4 | 15 | 3 | 6 | 0 | 1 | 5 | 6 |
Unique Cell Count | 424 | 415 | 257 | 284 | 357 | 492 | 648 | 598 | 532 | 488 | 629 | 697 | 151 | 151 | 104 | 663 | 624 | 433 | 424 | 426 | 248 | 144 | 198 | 193 |
Labelled Cell Count | 433 | 427 | 272 | 327 | 380 | 605 | 785 | 739 | 724 | 642 | 813 | 880 | 160 | 161 | 109 | 734 | 670 | 488 | 424 | 426 | 248 | 144 | 198 | 193 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 6.5 | 5.3 | 6.3 | 6.3 | 5.9 | 4.9 | 4.4 | 4.4 | 4.2 | 3.6 | 3.8 | 3.8 | 6.2 | 6.2 | 6.1 | 6.4 | 7.0 | 8.1 | 7.0 | 6.7 | 7.4 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.7 | 1.7 | 1.7 | 1.8 | 1.4 | 1.4 | 1.3 | 1.1 | 1.4 | 0.9 | 0.8 | 0.8 | 0.8 | 1.3 | 1.4 | 2.0 | 1.5 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.09 | -0.36 | -0.53 | -0.53 | -0.58 | -0.83 | -0.73 | -0.75 | -0.03 | -0.03 | -0.05 | 0.01 | 0.14 | 0.37 | 0.14 | 0.09 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.6 | 0 | 0.2 | 0.3 | 1.0 |
Cytoplasm | -2.3 | -3.8 | -5.9 | -6.8 | -8.3 | -8.5 | -10.7 | -10.7 | -11.2 | -1.1 | -1.1 | -0.2 | -1.3 | -2.1 | -2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.6 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.9 | 2.2 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | -0.6 | -1.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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