Standard name | |
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Human Ortholog | |
Description | Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 7 | 1 | 3 | 2 | 1 | 4 | 6 | 25 | 12 | 20 | 20 | 2 | 2 | 0 | 4 | 0 | 3 | 1 | 5 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 2 | 6 | 4 | 6 | 7 | 10 | 7 | 7 | 5 | 12 | 18 | 8 | 8 | 4 | 17 | 17 | 16 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 416 | 407 | 255 | 273 | 331 | 423 | 535 | 455 | 397 | 313 | 408 | 439 | 148 | 148 | 103 | 650 | 604 | 415 | 407 | 392 | 234 | 128 | 168 | 167 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 3 | 4 | 2 | 1 | 2 | 2 | 0 | 11 | 0 | 1 | 1 | 22 | 21 | 23 | 1 | 1 | 4 | 1 | 4 | 1 |
Endosome | 0 | 0 | 0 | 0 | 4 | 5 | 4 | 12 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 3 | 5 | 3 | 0 | 3 | 0 | 1 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 |
Mitochondria | 3 | 7 | 8 | 44 | 26 | 150 | 214 | 239 | 274 | 298 | 364 | 371 | 0 | 0 | 0 | 27 | 15 | 8 | 3 | 5 | 1 | 3 | 10 | 6 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 4 | 0 | 0 | 2 | 2 | 2 | 1 | 1 | 2 | 4 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 4 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 6 | 2 | 0 | 2 | 7 | 10 | 12 | 14 | 8 | 7 | 5 | 9 | 0 | 0 | 0 | 5 | 4 | 15 | 3 | 6 | 0 | 1 | 5 | 6 |
Unique Cell Count | 424 | 415 | 257 | 284 | 357 | 492 | 648 | 598 | 532 | 488 | 629 | 697 | 151 | 151 | 104 | 663 | 624 | 433 | 424 | 426 | 248 | 144 | 198 | 193 |
Labelled Cell Count | 433 | 427 | 272 | 327 | 380 | 605 | 785 | 739 | 724 | 642 | 813 | 880 | 160 | 161 | 109 | 734 | 670 | 488 | 424 | 426 | 248 | 144 | 198 | 193 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 5.3 | 6.3 | 6.3 | 5.9 | 4.9 | 4.4 | 4.4 | 4.2 | 3.6 | 3.8 | 3.8 | 6.2 | 6.2 | 6.1 | 6.4 | 7.0 | 8.1 | 7.0 | 6.7 | 7.4 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.7 | 1.7 | 1.7 | 1.8 | 1.4 | 1.4 | 1.3 | 1.1 | 1.4 | 0.9 | 0.8 | 0.8 | 0.8 | 1.3 | 1.4 | 2.0 | 1.5 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.09 | -0.36 | -0.53 | -0.53 | -0.58 | -0.83 | -0.73 | -0.75 | -0.03 | -0.03 | -0.05 | 0.01 | 0.14 | 0.37 | 0.14 | 0.09 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.6 | 0 | 0.2 | 0.3 | 1.0 |
Cytoplasm | -2.3 | -3.8 | -5.9 | -6.8 | -8.3 | -8.5 | -10.7 | -10.7 | -11.2 | -1.1 | -1.1 | -0.2 | -1.3 | -2.1 | -2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.6 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.9 | 2.2 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | -0.6 | -1.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.979 | 12.752 | 9.5809 | 9.409 | 9.2588 | 10.6715 | 8.7526 | 14.6424 | 12.3162 | 10.1785 | 11.0297 | 12.296 | 11.7018 | 12.6377 | 13.0528 | 10.6882 | 9.7067 | 12.3428 |
Actin | 0.0133 | 0.0019 | 0.0027 | 0.0035 | 0.0068 | 0.003 | 0.042 | 0.001 | 0.0272 | 0.0178 | 0.0651 | 0.0014 | 0.0001 | 0.0119 | 0.0073 | 0.0003 | 0.0012 | 0.0012 |
Bud | 0.0007 | 0.0043 | 0.0053 | 0.0015 | 0.0017 | 0.001 | 0.0016 | 0.0007 | 0.0024 | 0.0057 | 0.0018 | 0.0004 | 0 | 0.0076 | 0.0009 | 0.0006 | 0.0002 | 0.0002 |
Bud Neck | 0.001 | 0.0003 | 0.0005 | 0.0055 | 0.0009 | 0.0009 | 0.001 | 0.0006 | 0.0005 | 0.0028 | 0.0005 | 0.016 | 0 | 0.0017 | 0.0005 | 0.0003 | 0.0004 | 0.0007 |
Bud Periphery | 0.0006 | 0.0041 | 0.0038 | 0.0018 | 0.0043 | 0.0006 | 0.0022 | 0.0002 | 0.0022 | 0.0256 | 0.0019 | 0.0003 | 0 | 0.0022 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0014 | 0.0032 | 0.004 | 0.0025 | 0.0009 | 0.0001 | 0.0078 | 0.0015 | 0.0142 | 0.0132 | 0.0015 | 0.0001 | 0 | 0.1053 | 0.0019 | 0.0003 | 0.0003 | 0.0001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0001 | 0.0011 | 0.0007 | 0.0005 | 0.0051 | 0.0003 | 0.0003 | 0 | 0.001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.7918 | 0.9488 | 0.8632 | 0.7657 | 0.8444 | 0.8949 | 0.5901 | 0.9425 | 0.8127 | 0.7974 | 0.8093 | 0.8982 | 0.9951 | 0.8247 | 0.7776 | 0.9382 | 0.6998 | 0.9183 |
Cytoplasmic Foci | 0.028 | 0.0075 | 0.0181 | 0.053 | 0.0191 | 0.0128 | 0.0423 | 0.0094 | 0.0162 | 0.0126 | 0.0128 | 0.0082 | 0.0013 | 0.0105 | 0.0209 | 0.0086 | 0.0207 | 0.0094 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0364 | 0.0087 | 0.0097 | 0.0147 | 0.0175 | 0.0333 | 0.0252 | 0.0125 | 0.0234 | 0.0045 | 0.0042 | 0.0276 | 0.0008 | 0.0137 | 0.0093 | 0.0037 | 0.0027 | 0.0345 |
Endosome | 0.0477 | 0.0113 | 0.0409 | 0.0622 | 0.0431 | 0.034 | 0.1182 | 0.0182 | 0.0228 | 0.0705 | 0.0573 | 0.0196 | 0.0007 | 0.0086 | 0.0753 | 0.0304 | 0.0315 | 0.0238 |
Golgi | 0.0087 | 0.0011 | 0.0056 | 0.005 | 0.0036 | 0.0038 | 0.0169 | 0.0033 | 0.0159 | 0.0021 | 0.0172 | 0.0035 | 0 | 0.0028 | 0.0085 | 0.0006 | 0.0083 | 0.0023 |
Lipid Particles | 0.0047 | 0 | 0.0019 | 0.0066 | 0.0033 | 0.0013 | 0.0142 | 0.0002 | 0.002 | 0.0001 | 0.0019 | 0.0001 | 0 | 0.0004 | 0.0014 | 0.0001 | 0.136 | 0 |
Mitochondria | 0.0083 | 0.0006 | 0.0151 | 0.001 | 0.0026 | 0.0036 | 0.0311 | 0.0012 | 0.0337 | 0.0005 | 0.0062 | 0.0163 | 0 | 0.0005 | 0.0423 | 0.0002 | 0.0186 | 0.0031 |
None | 0.0076 | 0.0019 | 0.0076 | 0.0024 | 0.0011 | 0.0012 | 0.0103 | 0.0027 | 0.0041 | 0.0042 | 0.0033 | 0.0009 | 0.0008 | 0.0023 | 0.0016 | 0.002 | 0.0003 | 0.0011 |
Nuclear Periphery | 0.0059 | 0.0007 | 0.0019 | 0.0019 | 0.0056 | 0.0019 | 0.0369 | 0.0011 | 0.0052 | 0.0017 | 0.0028 | 0.0013 | 0.0002 | 0.002 | 0.0025 | 0.0014 | 0.0012 | 0.001 |
Nucleolus | 0.0004 | 0 | 0.0016 | 0.0003 | 0.0031 | 0 | 0.006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0047 | 0 | 0.0002 | 0 |
Nucleus | 0.004 | 0.001 | 0.0018 | 0.0015 | 0.0128 | 0.0009 | 0.0167 | 0.0008 | 0.0013 | 0.0015 | 0.0011 | 0.0008 | 0.0003 | 0.0012 | 0.0074 | 0.0013 | 0.0012 | 0.0008 |
Peroxisomes | 0.011 | 0.0001 | 0.001 | 0.0138 | 0.019 | 0.0009 | 0.003 | 0.0001 | 0.0047 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0006 | 0.0009 | 0 | 0.0684 | 0 |
Punctate Nuclear | 0.0098 | 0.0001 | 0.0016 | 0.002 | 0.0011 | 0.0004 | 0.0105 | 0.0001 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0267 | 0.0002 | 0.0004 | 0.0001 |
Vacuole | 0.0147 | 0.0036 | 0.0105 | 0.0476 | 0.0062 | 0.0036 | 0.0168 | 0.0027 | 0.0073 | 0.0265 | 0.0073 | 0.0038 | 0.0005 | 0.0019 | 0.0072 | 0.0091 | 0.006 | 0.0021 |
Vacuole Periphery | 0.0029 | 0.0006 | 0.0028 | 0.0063 | 0.0027 | 0.0017 | 0.0057 | 0.0006 | 0.0029 | 0.0075 | 0.0038 | 0.001 | 0 | 0.0004 | 0.0026 | 0.0024 | 0.0024 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.5905 | 33.6409 | 32.028 | 47.1096 | 30.1496 | 41.5144 | 35.6187 | 33.4614 | 36.1202 | 31.0677 |
Translational Efficiency | 0.746 | 0.7703 | 0.8368 | 0.6876 | 0.5615 | 0.9083 | 0.5922 | 0.4775 | 0.6133 | 0.5186 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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