Standard name
Human Ortholog
Description Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.96 0.93 0.86 0.83 0.76 0.75 0.64 0.65 0.63 0.98 0.98 0.99 0.98 0.97 0.96 0.96 0.92 0.94 0.89 0.85 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.15 0.07 0.3 0.33 0.4 0.52 0.61 0.58 0.53 0 0 0 0 0 0 0 0 0 0 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 0 0 1 0 1 0 0 0 0 3 1 1 0 1 0 0 0 1
Bud 0 7 1 3 2 1 4 6 25 12 20 20 2 2 0 4 0 3 1 5 1 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0
Cell Periphery 8 2 6 4 6 7 10 7 7 5 12 18 8 8 4 17 17 16 0 0 0 0 0 0
Cytoplasm 416 407 255 273 331 423 535 455 397 313 408 439 148 148 103 650 604 415 407 392 234 128 168 167
Endoplasmic Reticulum 0 2 1 0 3 4 2 1 2 2 0 11 0 1 1 22 21 23 1 1 4 1 4 1
Endosome 0 0 0 0 4 5 4 12 0 2 0 2 0 0 0 3 5 3 0 3 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 1 1 0
Mitochondria 3 7 8 44 26 150 214 239 274 298 364 371 0 0 0 27 15 8 3 5 1 3 10 6
Nucleus 0 0 0 1 0 2 1 1 4 0 0 2 2 2 1 1 2 4 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 1 0 2 3 4 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 2 0 2 2 0 0 0 1 0 0 0 1 0 0 1 1
Vac/Vac Membrane 6 2 0 2 7 10 12 14 8 7 5 9 0 0 0 5 4 15 3 6 0 1 5 6
Unique Cell Count 424 415 257 284 357 492 648 598 532 488 629 697 151 151 104 663 624 433 424 426 248 144 198 193
Labelled Cell Count 433 427 272 327 380 605 785 739 724 642 813 880 160 161 109 734 670 488 424 426 248 144 198 193


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.3 6.3 6.3 5.9 4.9 4.4 4.4 4.2 3.6 3.8 3.8 6.2 6.2 6.1 6.4 7.0 8.1 7.0 6.7 7.4
Std Deviation (1e-4) 1.3 1.0 1.7 1.7 1.7 1.8 1.4 1.4 1.3 1.1 1.4 0.9 0.8 0.8 0.8 1.3 1.4 2.0 1.5 1.9 2.1
Intensity Change (Log2) -0.01 -0.09 -0.36 -0.53 -0.53 -0.58 -0.83 -0.73 -0.75 -0.03 -0.03 -0.05 0.01 0.14 0.37 0.14 0.09 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.6 0 0 0 0 0 0 0 1.6 1.6 0 0.2 0.3 1.0
Cytoplasm -2.3 -3.8 -5.9 -6.8 -8.3 -8.5 -10.7 -10.7 -11.2 -1.1 -1.1 -0.2 -1.3 -2.1 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.6 2.6 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.9 2.2 8.7 0 0 0 0 0 0 0 0 0 0.7 -0.6 -1.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.979 12.752 9.5809 9.409 9.2588 10.6715 8.7526 14.6424 12.3162 10.1785 11.0297 12.296 11.7018 12.6377 13.0528 10.6882 9.7067 12.3428
Actin 0.0133 0.0019 0.0027 0.0035 0.0068 0.003 0.042 0.001 0.0272 0.0178 0.0651 0.0014 0.0001 0.0119 0.0073 0.0003 0.0012 0.0012
Bud 0.0007 0.0043 0.0053 0.0015 0.0017 0.001 0.0016 0.0007 0.0024 0.0057 0.0018 0.0004 0 0.0076 0.0009 0.0006 0.0002 0.0002
Bud Neck 0.001 0.0003 0.0005 0.0055 0.0009 0.0009 0.001 0.0006 0.0005 0.0028 0.0005 0.016 0 0.0017 0.0005 0.0003 0.0004 0.0007
Bud Periphery 0.0006 0.0041 0.0038 0.0018 0.0043 0.0006 0.0022 0.0002 0.0022 0.0256 0.0019 0.0003 0 0.0022 0.0003 0.0002 0.0001 0.0001
Bud Site 0.0014 0.0032 0.004 0.0025 0.0009 0.0001 0.0078 0.0015 0.0142 0.0132 0.0015 0.0001 0 0.1053 0.0019 0.0003 0.0003 0.0001
Cell Periphery 0.0005 0.0002 0.0003 0.0013 0.0002 0.0001 0.0011 0.0007 0.0005 0.0051 0.0003 0.0003 0 0.001 0.0002 0.0001 0 0.0001
Cytoplasm 0.7918 0.9488 0.8632 0.7657 0.8444 0.8949 0.5901 0.9425 0.8127 0.7974 0.8093 0.8982 0.9951 0.8247 0.7776 0.9382 0.6998 0.9183
Cytoplasmic Foci 0.028 0.0075 0.0181 0.053 0.0191 0.0128 0.0423 0.0094 0.0162 0.0126 0.0128 0.0082 0.0013 0.0105 0.0209 0.0086 0.0207 0.0094
Eisosomes 0.0004 0 0.0001 0 0 0 0.0005 0 0.0002 0.0006 0.0001 0 0 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0364 0.0087 0.0097 0.0147 0.0175 0.0333 0.0252 0.0125 0.0234 0.0045 0.0042 0.0276 0.0008 0.0137 0.0093 0.0037 0.0027 0.0345
Endosome 0.0477 0.0113 0.0409 0.0622 0.0431 0.034 0.1182 0.0182 0.0228 0.0705 0.0573 0.0196 0.0007 0.0086 0.0753 0.0304 0.0315 0.0238
Golgi 0.0087 0.0011 0.0056 0.005 0.0036 0.0038 0.0169 0.0033 0.0159 0.0021 0.0172 0.0035 0 0.0028 0.0085 0.0006 0.0083 0.0023
Lipid Particles 0.0047 0 0.0019 0.0066 0.0033 0.0013 0.0142 0.0002 0.002 0.0001 0.0019 0.0001 0 0.0004 0.0014 0.0001 0.136 0
Mitochondria 0.0083 0.0006 0.0151 0.001 0.0026 0.0036 0.0311 0.0012 0.0337 0.0005 0.0062 0.0163 0 0.0005 0.0423 0.0002 0.0186 0.0031
None 0.0076 0.0019 0.0076 0.0024 0.0011 0.0012 0.0103 0.0027 0.0041 0.0042 0.0033 0.0009 0.0008 0.0023 0.0016 0.002 0.0003 0.0011
Nuclear Periphery 0.0059 0.0007 0.0019 0.0019 0.0056 0.0019 0.0369 0.0011 0.0052 0.0017 0.0028 0.0013 0.0002 0.002 0.0025 0.0014 0.0012 0.001
Nucleolus 0.0004 0 0.0016 0.0003 0.0031 0 0.006 0 0.0001 0.0001 0.0001 0 0 0.0001 0.0047 0 0.0002 0
Nucleus 0.004 0.001 0.0018 0.0015 0.0128 0.0009 0.0167 0.0008 0.0013 0.0015 0.0011 0.0008 0.0003 0.0012 0.0074 0.0013 0.0012 0.0008
Peroxisomes 0.011 0.0001 0.001 0.0138 0.019 0.0009 0.003 0.0001 0.0047 0.0001 0.0011 0.0001 0 0.0006 0.0009 0 0.0684 0
Punctate Nuclear 0.0098 0.0001 0.0016 0.002 0.0011 0.0004 0.0105 0.0001 0.0007 0.0002 0.0004 0.0001 0 0.0005 0.0267 0.0002 0.0004 0.0001
Vacuole 0.0147 0.0036 0.0105 0.0476 0.0062 0.0036 0.0168 0.0027 0.0073 0.0265 0.0073 0.0038 0.0005 0.0019 0.0072 0.0091 0.006 0.0021
Vacuole Periphery 0.0029 0.0006 0.0028 0.0063 0.0027 0.0017 0.0057 0.0006 0.0029 0.0075 0.0038 0.001 0 0.0004 0.0026 0.0024 0.0024 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.5905 33.6409 32.028 47.1096 30.1496 41.5144 35.6187 33.4614 36.1202 31.0677
Translational Efficiency 0.746 0.7703 0.8368 0.6876 0.5615 0.9083 0.5922 0.4775 0.6133 0.5186

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide
Localization
Cell Percentages cytoplasm (78%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Met10

Met10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available