Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.98 0.98 0.88 0.91 0.79 0.74 0.76 0.82 0.78 0.99 0.99 0.99 0.97 0.93 0.93 0.94 0.86 0.94 0.89 0.88 0.88
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0.09 0.09 0.06 0 0.07 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.11 0.09 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.07 0.06 0.08 0.08 0.06 0.05 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 2 1 3 0 0 1 0 0 0 0 1 0 0 0 1
Bud 1 3 0 0 0 0 0 2 0 2 0 1 1 0 0 0 0 0 4 27 0 10 15 24
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 2 0 0 0 1 2 2 4 3 13 4 5 9 1 9 5 0 0 0 0 3 0
Cytoplasm 247 384 59 51 41 74 117 146 74 96 93 130 290 404 471 180 187 138 223 342 62 255 310 359
Endoplasmic Reticulum 3 5 0 0 0 4 0 17 9 7 4 11 2 3 5 6 17 5 3 6 0 5 4 5
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Golgi 0 0 0 1 0 0 0 3 2 1 2 3 0 0 1 1 8 2 0 1 0 0 0 0
Mitochondria 0 0 0 0 1 0 6 3 11 11 10 11 0 0 0 5 2 0 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 0 2 1 1 3 1 0 0 2 0 1 0 0 2 0 3 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 1 0 2 6 8 15 8 8 6 9 0 1 0 2 5 7 1 8 0 4 6 4
Unique Cell Count 250 393 59 52 42 84 129 185 100 127 113 166 292 407 477 185 202 148 239 399 66 286 352 408
Labelled Cell Count 252 400 62 52 44 84 133 190 108 134 122 183 299 413 490 195 229 157 239 399 66 286 352 408


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 115.4 61.3 69.3 81.3 29.8 39.6 17.5 27.5 21.2 21.2 21.5 22.9 79.9 68.9 66.1 93.9 115.5 132.9 74.8 78.1 81.1
Std Deviation (1e-4) 42.1 36.0 41.7 36.5 15.9 20.1 9.1 12.8 10.3 10.3 10.3 10.4 38.2 36.9 34.7 50.5 63.8 63.0 41.1 43.0 43.3
Intensity Change (Log2) – – – 0.23 -1.22 -0.81 -1.98 -1.33 -1.71 -1.71 -1.69 -1.6 0.2 -0.01 -0.07 0.44 0.74 0.94 0.11 0.17 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.2 -2.7 -2.4 -3.8 -4.3 -4.2 -3.4 -3.9 -0.6 -0.7 -0.9 -1.3 -2.2 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 109.7584 128.7963 126.2251 109.158 88.8385 113.2454 111.0613 130.2648 125.1382 110.0053 94.6784 130.0419 102.47 126.2017 110.4482 110.1682 100.6615 115.6958
Actin 0.0031 0.0006 0.0005 0.0003 0.0068 0.0034 0.0038 0.0003 0.0021 0.0009 0.0062 0.0011 0.0086 0.0002 0.0035 0.0009 0.049 0.0005
Bud 0.0014 0.0008 0.0017 0.002 0.0051 0.0017 0.0046 0.0004 0.0021 0.0036 0.0062 0.0004 0.0017 0.0003 0.0011 0.0011 0.0016 0.0004
Bud Neck 0.0011 0.0008 0.0009 0.0008 0.001 0.0028 0.0014 0.0005 0.0012 0.0009 0.0017 0.004 0.0011 0.0004 0.0012 0.0009 0.0005 0.0009
Bud Periphery 0.0018 0.0007 0.0009 0.0011 0.0045 0.0017 0.0041 0.0002 0.0014 0.0024 0.0053 0.0001 0.0029 0.0001 0.0009 0.0008 0.0006 0.0002
Bud Site 0.0014 0.0006 0.0006 0.0004 0.0033 0.0019 0.0024 0.0004 0.0017 0.001 0.0019 0.0001 0.001 0.0003 0.0007 0.0005 0.0009 0.0001
Cell Periphery 0.0256 0.0147 0.0207 0.0055 0.0045 0.041 0.0384 0.0078 0.0182 0.0288 0.0125 0.0004 0.0346 0.0067 0.0156 0.0435 0.0018 0.0062
Cytoplasm 0.6432 0.9046 0.7663 0.5789 0.7214 0.7136 0.6732 0.9522 0.8073 0.6205 0.6809 0.9066 0.6681 0.9547 0.8247 0.626 0.704 0.8911
Cytoplasmic Foci 0.0045 0.0013 0.0015 0.0036 0.0187 0.0167 0.012 0.0008 0.0039 0.003 0.0123 0.0063 0.0153 0.0007 0.0074 0.01 0.0066 0.0024
Eisosomes 0.0003 0 0 0.0004 0.0002 0.0002 0.0002 0 0.0003 0.0005 0.0001 0 0.0003 0 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.076 0.0261 0.0081 0.0122 0.0019 0.0559 0.0461 0.0087 0.0151 0.0041 0.0057 0.0121 0.0414 0.0079 0.009 0.0028 0.0119 0.0071
Endosome 0.0119 0.003 0.0042 0.0066 0.0373 0.0318 0.0096 0.0014 0.0033 0.0044 0.0451 0.0439 0.0132 0.001 0.0124 0.0371 0.0512 0.0041
Golgi 0.0029 0.0011 0.0003 0.0006 0.0177 0.0057 0.0017 0.0002 0.0004 0.0003 0.0068 0.0036 0.0051 0.0002 0.0036 0.0266 0.0121 0.0003
Lipid Particles 0.0038 0.0021 0.0008 0.0023 0.0238 0.0076 0.0041 0.0005 0.0018 0.0037 0.0035 0.0005 0.013 0.0003 0.0071 0.0073 0.0041 0.0015
Mitochondria 0.0084 0.0003 0.0004 0.0294 0.02 0.0038 0.0063 0.0001 0.0023 0.012 0.0135 0.0015 0.0114 0.0001 0.0062 0.0035 0.0023 0.0007
None 0.0041 0.0001 0.0003 0.0052 0.0047 0.0028 0.0039 0.0002 0.0062 0.009 0.0044 0.0002 0.0057 0.0001 0.0113 0.0002 0.0011 0.0005
Nuclear Periphery 0.0368 0.0048 0.0134 0.0057 0.0063 0.0136 0.0322 0.002 0.0082 0.0102 0.0085 0.0041 0.0319 0.0024 0.0061 0.0082 0.0141 0.0249
Nucleolus 0.0026 0.0001 0.0005 0.0041 0.0266 0.0036 0.0031 0.0003 0.002 0.0061 0.0066 0 0.0026 0.0001 0.0064 0.0005 0.0004 0.0007
Nucleus 0.0096 0.0014 0.006 0.006 0.0104 0.006 0.0082 0.0016 0.0058 0.0104 0.0135 0.002 0.0098 0.0013 0.0052 0.005 0.0049 0.0084
Peroxisomes 0.0012 0 0.0001 0.0012 0.0121 0.0033 0.0006 0 0.0003 0.0005 0.0013 0.0001 0.0036 0 0.0042 0.0027 0.0016 0
Punctate Nuclear 0.0007 0 0.0001 0.0001 0.0034 0.0014 0.0012 0 0.0002 0.0002 0.0012 0.0001 0.0036 0 0.0006 0.0002 0.0007 0.0002
Vacuole 0.1238 0.0327 0.1451 0.2542 0.0502 0.066 0.1196 0.0203 0.099 0.2011 0.1298 0.0085 0.1025 0.0217 0.0575 0.1809 0.0978 0.039
Vacuole Periphery 0.0358 0.004 0.0274 0.0794 0.0204 0.0154 0.0231 0.002 0.0172 0.0761 0.0332 0.0044 0.0225 0.0015 0.0151 0.0414 0.0326 0.0108

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2118.825 2320.8851 2539.2836 2307.9416 2723.7732 2098.9117 2111.0023 2164.1235 2360.1266 2004.1865
Translational Efficiency 1.3798 1.2902 1.2771 1.1533 1.1848 1.2778 1.3346 1.3156 1.1342 1.3231

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2317 845 220 1772 1287 1567 1923 1671 3604 2412 2143 3443

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 6350.58 7774.78 9237.55 6455.99 6317.91 7441.30 7314.55 6935.65 6338.91 7558.13 7511.96 6688.78
Standard Deviation 1971.48 1684.21 1720.55 2295.13 1527.33 1560.23 2205.53 2166.22 1825.39 1612.62 2238.20 2246.32
Intensity Change Log 2 — 0.291913 0.540622 0.023750 — 0.236107 0.211322 0.134584 — 0.264352 0.385770 0.080088

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000107 0.000125 0.000122 0.000346 0.000088 0.000129 0.000131 0.000501 0.000100 0.000128 0.000130 0.000422
Bud Neck 0.000438 0.000830 0.006021 0.002605 0.000820 0.000581 0.001724 0.001297 0.000574 0.000668 0.002165 0.001970
Bud Site 0.006118 0.006813 0.004544 0.017694 0.002518 0.004986 0.005980 0.011110 0.004833 0.005626 0.005833 0.014499
Cell Periphery 0.020022 0.046859 0.036928 0.017190 0.044151 0.035471 0.020724 0.062065 0.028638 0.039461 0.022388 0.038969
Cytoplasm 0.673199* 0.478415* 0.136865* 0.616430* 0.493549* 0.571084* 0.614672* 0.602505* 0.609046* 0.538619* 0.565621* 0.609672*
Cytoplasmic Foci 0.010671 0.011959 0.011294 0.009043 0.007638 0.009780 0.006283 0.007995 0.009588 0.010543 0.006797 0.008535
Eisosomes 0.000002 0.000003 0.000010 0.000005 0.000003 0.000003 0.000003 0.000005 0.000002 0.000003 0.000003 0.000005
Endoplasmic Reticulum 0.001847 0.000939 0.000263 0.000548 0.001418 0.001231 0.000573 0.000541 0.001694 0.001129 0.000541 0.000544
Endosome 0.000902 0.001060 0.001065 0.001733 0.000606 0.000944 0.000736 0.000744 0.000796 0.000984 0.000770 0.001253
Golgi 0.052822 0.091171 0.049348 0.016380 0.070134 0.076021 0.020194 0.022777 0.059004 0.081328 0.023187 0.019485
Lipid Particles 0.021403 0.029120 0.023494 0.016618 0.024960 0.027793 0.014151 0.037559 0.022674 0.028258 0.015110 0.026782
Mitochondria 0.000458 0.000245 0.000104 0.001014 0.000257 0.000236 0.000343 0.000378 0.000386 0.000240 0.000319 0.000706
Mitotic Spindle 0.000811 0.001346 0.001494 0.002055 0.000546 0.001287 0.001485 0.001630 0.000716 0.001307 0.001486 0.001849
None 0.003498 0.001903 0.000612 0.003166 0.001760 0.001559 0.001632 0.001056 0.002877 0.001680 0.001527 0.002142
Nuclear Periphery 0.000507 0.001069 0.003360 0.000364 0.001307 0.000711 0.000673 0.000559 0.000793 0.000837 0.000949 0.000459
Nuclear Periphery Foci 0.009718 0.014092 0.015311 0.005914 0.013963 0.012576 0.007413 0.006660 0.011234 0.013107 0.008224 0.006276
Nucleolus 0.000089 0.000088 0.000178 0.000305 0.000123 0.000093 0.000173 0.000116 0.000101 0.000091 0.000173 0.000214
Nucleus 0.005350 0.004634 0.010825 0.013466 0.007849 0.005395 0.019295 0.012279 0.006242 0.005129 0.018425 0.012890
Peroxisomes 0.000052 0.000031 0.000023 0.000228 0.000025 0.000038 0.000044 0.000112 0.000042 0.000035 0.000042 0.000171
Vacuole 0.106447 0.182887 0.558335* 0.233105* 0.186625 0.154006 0.232731* 0.198796 0.135079 0.164124 0.266157* 0.216454*
Vacuole Periphery 0.085540 0.126409* 0.139804* 0.041791 0.141661* 0.096075 0.051040 0.031312 0.105581* 0.106702* 0.060152 0.036705

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.30 -0.97 -3.22 -3.19 -2.93 -2.74 -4.80 -2.66 -2.40 -2.15 -1.18 -3.00 -3.82 -3.62 -3.18
Bud Neck -2.51 -5.21 -8.96 -7.63 3.06 0.99 -5.36 -2.78 -7.48 4.04 -0.57 -8.73 -9.57 -10.07 2.22
Bud Site -0.60 0.57 -8.96 -7.38 -7.96 -3.31 -6.47 -8.97 -6.23 -3.88 -0.96 -3.03 -11.51 -10.34 -8.56
Cell Periphery -8.88 -4.55 3.54 10.50 6.03 3.24 10.66 -3.91 -7.05 -13.64 -5.92 5.62 -3.83 1.88 -8.38
Cytoplasm 13.55 29.45 6.65 -8.29 -24.88 -5.71 -8.23 -8.12 -2.40 -0.21 7.46 5.88 0.91 -6.68 -5.12
Cytoplasmic Foci -1.11 -1.28 -0.48 0.62 0.98 -1.90 0.14 -2.55 -1.28 -2.89 -1.13 1.83 -1.79 -0.85 -3.41
Eisosomes -1.02 -5.94 -2.40 -2.03 2.59 -0.48 -2.04 -5.88 -5.27 -4.42 -0.92 -3.77 -4.15 -3.85 -2.28
Endoplasmic Reticulum 5.18 10.10 7.88 3.88 -2.90 0.91 5.12 5.28 6.75 0.47 4.15 9.76 9.64 7.52 0.10
Endosome -1.66 -1.35 -3.10 -2.43 -1.70 -5.13 -3.27 -1.32 1.86 0.67 -3.41 -0.91 -3.09 -1.77 -2.66
Golgi -9.46 1.76 19.22 20.97 9.23 -1.74 20.59 18.92 20.05 -1.72 -8.81 22.21 25.80 29.36 3.65
Lipid Particles -3.49 -1.29 2.60 5.42 3.26 -1.35 6.41 -4.96 -3.94 -10.58 -3.78 6.52 -3.00 0.60 -8.64
Mitochondria 3.70 7.13 -1.72 -2.57 -3.03 0.40 -2.51 -0.97 -1.17 0.34 3.87 0.34 -1.77 -2.83 -1.86
Mitotic Spindle -3.02 -1.41 -5.09 -4.42 -1.30 -4.32 -2.79 -3.22 -1.94 -0.97 -4.44 -2.74 -5.94 -4.74 -2.91
None 2.00 5.60 0.72 -1.21 -3.73 0.89 0.20 3.69 2.11 2.74 2.94 3.47 1.81 -0.97 -1.27
Nuclear Periphery -7.03 -7.86 1.52 7.79 8.04 4.97 4.50 6.38 3.30 3.67 -0.88 -3.36 5.80 8.20 8.61
Nuclear Periphery Foci -4.91 -4.14 5.67 8.54 6.70 1.67 8.41 10.09 9.23 1.38 -3.45 5.28 10.50 12.65 3.49
Nucleolus 0.29 -2.86 -4.95 -5.66 -2.52 1.12 -2.88 0.18 -2.05 4.93 0.73 -4.41 -4.48 -5.88 -1.05
Nucleus 1.00 -3.18 -11.77 -11.67 -2.87 2.29 -10.69 -5.72 -8.97 6.44 1.98 -12.89 -13.30 -14.62 4.30
Peroxisomes 2.97 2.15 -1.97 -2.26 -2.26 -2.62 -3.63 -5.67 -4.73 -4.24 1.53 0.05 -2.84 -3.04 -2.85
Vacuole -10.11 -24.79 -18.29 -5.53 16.85 4.22 -6.52 -1.48 -5.88 5.16 -6.00 -20.35 -15.93 -9.04 7.45
Vacuole Periphery -6.84 -4.36 15.27 16.52 9.77 8.03 18.97 23.28 18.84 7.83 -0.21 15.89 26.97 24.48 10.09
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl2a

Rpl2a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl2a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available