Standard name
Description Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.98 0.98 0.88 0.91 0.79 0.74 0.76 0.82 0.78 0.99 0.99 0.99 0.97 0.93 0.93 0.94 0.86 0.94 0.89 0.88 0.88
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0.09 0.09 0.06 0 0.07 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.11 0.09 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.07 0.06 0.08 0.08 0.06 0.05 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 2 1 3 0 0 1 0 0 0 0 1 0 0 0 1
Bud 1 3 0 0 0 0 0 2 0 2 0 1 1 0 0 0 0 0 4 27 0 10 15 24
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 2 0 0 0 1 2 2 4 3 13 4 5 9 1 9 5 0 0 0 0 3 0
Cytoplasm 247 384 59 51 41 74 117 146 74 96 93 130 290 404 471 180 187 138 223 342 62 255 310 359
Endoplasmic Reticulum 3 5 0 0 0 4 0 17 9 7 4 11 2 3 5 6 17 5 3 6 0 5 4 5
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Golgi 0 0 0 1 0 0 0 3 2 1 2 3 0 0 1 1 8 2 0 1 0 0 0 0
Mitochondria 0 0 0 0 1 0 6 3 11 11 10 11 0 0 0 5 2 0 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 0 2 1 1 3 1 0 0 2 0 1 0 0 2 0 3 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 1 0 2 6 8 15 8 8 6 9 0 1 0 2 5 7 1 8 0 4 6 4
Unique Cell Count 250 393 59 52 42 84 129 185 100 127 113 166 292 407 477 185 202 148 239 399 66 286 352 408
Labelled Cell Count 252 400 62 52 44 84 133 190 108 134 122 183 299 413 490 195 229 157 239 399 66 286 352 408


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 115.4 61.3 69.3 81.3 29.8 39.6 17.5 27.5 21.2 21.2 21.5 22.9 79.9 68.9 66.1 93.9 115.5 132.9 74.8 78.1 81.1
Std Deviation (1e-4) 42.1 36.0 41.7 36.5 15.9 20.1 9.1 12.8 10.3 10.3 10.3 10.4 38.2 36.9 34.7 50.5 63.8 63.0 41.1 43.0 43.3
Intensity Change (Log2) – – – 0.23 -1.22 -0.81 -1.98 -1.33 -1.71 -1.71 -1.69 -1.6 0.2 -0.01 -0.07 0.44 0.74 0.94 0.11 0.17 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.2 -2.7 -2.4 -3.8 -4.3 -4.2 -3.4 -3.9 -0.6 -0.7 -0.9 -1.3 -2.2 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0