Standard name
Description AAA-peroxin; heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis; mutations in human PEX1 can lead to severe peroxisomal disorders and early death

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.2 0.1 0.11 0.13 0.08 0.05 0 0.06 0 0 0 0.16 0.21 0.17 0 0 0 0.05 0.15 0.06 0.1 0.21 0.17
Bud 0.05 0.06 0.14 0.14 0.25 0.19 0.12 0.12 0.14 0.12 0.1 0.1 0.13 0.15 0.06 0.06 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.06 0.06 0 0.06 0.08 0 0.05 0.06 0 0 0 0 0.05 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.14 0.05 0 0 0.1 0.07 0.06 0.09 0 0 0 0.1 0.23 0.24 0.18 0.19 0.16 0.06 0.11 0.18 0.18 0.16 0.08
Golgi 0.11 0.06 0 0 0 0 0 0 0 0 0 0.13 0 0 0 0.05 0.05 0 0.06 0 0 0 0
Mitochondria 0.52 0.69 0.84 0.82 0.48 0.7 0.65 0.57 0.84 0.83 0.89 0.5 0.14 0.36 0.09 0.1 0.09 0 0.06 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Peroxisomes 0.53 0.36 0.27 0.33 0.53 0.28 0.3 0.27 0.15 0.12 0.12 0.49 0.54 0.31 0.51 0.54 0.38 0.79 0.54 0.59 0.5 0.47 0.64
SpindlePole 0.09 0.06 0.08 0.06 0.16 0.11 0.09 0.14 0.08 0.05 0.05 0.07 0.22 0.13 0.38 0.39 0.45 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 35 18 15 32 28 19 18 38 12 12 12 21 56 61 17 5 25 9 31 8 12 50 64
Bud 9 11 18 35 85 75 62 74 48 41 40 13 34 51 31 22 38 1 3 2 3 3 1
Bud Neck 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 6 2 0 3 3 2 5 3
Bud Site 4 2 8 14 10 22 39 22 17 21 11 4 3 11 24 11 31
Cell Periphery 1 5 1 2 1 1 5 5 2 1 2 1 8 4 2 0 2 0 0 0 0 0 0
Cytoplasm 1 6 2 9 12 13 26 29 11 13 9 5 14 25 21 15 30 0 1 1 1 1 0
Endoplasmic Reticulum 1 2 3 6 3 5 6 16 9 7 10 0 1 9 4 3 8 0 0 0 0 0 1
Endosome 25 9 0 5 35 27 30 56 6 2 1 13 62 85 93 65 111 12 22 25 23 39 31
Golgi 20 10 2 0 0 0 2 1 0 0 1 17 10 11 22 19 36 7 12 5 4 3 13
Mitochondria 91 124 110 199 164 276 328 346 288 276 372 66 38 126 48 35 62 3 12 3 6 8 13
Nucleus 0 0 0 0 0 2 2 1 1 2 4 0 0 1 3 3 8 0 0 0 0 0 0
Nuclear Periphery 5 6 0 1 1 5 12 3 3 10 6 3 1 0 11 4 14 0 0 0 0 0 1
Nucleolus 4 8 3 2 6 9 8 21 2 1 6 1 4 5 28 17 27 0 0 0 0 0 0
Peroxisomes 93 65 36 81 184 112 151 165 50 41 49 65 145 109 260 190 272 151 112 85 63 115 243
SpindlePole 16 10 11 14 55 43 45 84 27 18 19 9 60 46 195 138 317 4 4 5 5 10 5
Vac/Vac Membrane 1 5 0 0 2 0 2 4 0 0 4 1 2 4 10 6 10 0 1 1 0 2 0
Unique Cell Count 175 181 131 243 345 396 507 605 344 331 419 133 268 349 514 350 708 192 207 145 126 245 383
Labelled Cell Count 306 281 209 401 587 610 736 865 476 445 546 219 438 548 769 539 993 192 207 145 126 245 383


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 6.6 4.9 4.8 5.3 4.9 4.7 4.7 4.2 4.1 3.8 6.6 6.9 6.0 7.2 8.0 8.2 6.9 6.3 7.0
Std Deviation (1e-4) 1.1 0.9 0.7 1.0 1.2 1.2 1.1 1.1 0.9 0.8 0.8 1.0 1.1 1.0 1.5 1.9 1.8 1.7 1.3 1.2
Intensity Change (Log2) -0.03 0.11 0.0 -0.07 -0.07 -0.23 -0.28 -0.38 0.43 0.48 0.29 0.55 0.69 0.73 0.49 0.35 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 2.6 1.4 0 0 -1.0 -0.3 0.2 -3.0 -2.6 -3.5
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 -0.2 0 0 -1.2 0 -1.5 -0.7 -1.5 -0.9
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 3.8 3.1 0 0 3.7 6.0 6.2 5.3 5.3 4.9
Golgi 0 0 0 0 3.5 0 0 0 1.9 1.8
Mitochondria -7.2 -3.2 0 0 -5.9 -13.6 -9.3 -17.7 -15.7 -19.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 1.5 1.3 0
Peroxisomes 5.1 0.2 0 0 3.6 5.0 0.8 4.7 5.2 2.4
SpindlePole 2.1 0.8 0 0 -0.5 3.4 1.4 6.5 6.6 7.8
Vac
Cortical Patches -1.1 -2.7 0 0 1.0 2.3 1.6 -3.8 -4.9 -3.9
Cytoplasm 0 0 1.8 1.7 0 1.8 2.4 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available