Standard name
Human Ortholog
Description Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm – 0.98 1.0 0.97 0.98 0.91 0.84 0.77 0.73 0.75 0.75 0.73 0.97 0.96 0.97 0.98 0.93 0.97 1.0 0.95 0.95 0.84 0.83 0.76
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0.07 0.12 0.07 0.16 0.09 0.18 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0.06 0.14 0.12 0.14 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 1 5 1 2 0 0 0 0 0 0 0 0 0 0 2 3
Bud – 0 0 0 1 10 8 9 11 6 4 12 1 1 2 0 0 0 0 0 0 0 5 12
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 2 10 9 13 11 19 39 26 31 0 5 6 5 8 8 0 0 0 0 0 0
Cytoplasm – 159 51 260 567 341 487 367 330 515 305 403 112 233 285 209 136 152 7 151 61 62 172 227
Endoplasmic Reticulum – 1 0 1 3 7 13 2 13 31 11 18 1 3 5 6 7 6 0 0 2 4 3 7
Endosome – 0 0 1 0 0 0 0 0 0 1 0 0 4 1 0 0 1 0 0 0 1 5 6
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 1 0 0 0 2
Mitochondria – 2 0 2 11 26 67 35 71 61 74 114 0 0 0 2 0 1 0 1 0 1 1 4
Nucleus – 2 0 2 1 0 8 6 5 8 3 6 1 0 1 2 4 1 0 0 0 0 1 2
Nuclear Periphery – 0 0 1 0 0 1 2 3 1 4 3 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus – 0 0 1 0 0 0 3 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5
Vac/Vac Membrane – 1 0 3 4 9 35 68 56 94 42 44 2 5 3 1 4 5 0 1 0 0 7 12
Unique Cell Count 163 51 267 580 374 578 477 451 683 409 554 116 242 295 214 146 156 8 159 65 75 208 298
Labelled Cell Count 166 51 273 597 402 632 503 511 760 473 633 117 251 304 225 161 174 8 159 65 75 208 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 9.3 7.1 7.1 8.8 7.9 7.8 8.4 8.8 9.3 9.0 9.7 8.6 9.2 9.4 14.3 13.9 13.8 12.6 10.4 11.3
Std Deviation (1e-4) – 2.3 1.5 1.7 1.8 1.6 2.2 2.5 3.4 3.6 3.9 4.3 1.9 4.4 1.8 3.2 3.0 3.0 7.0 2.8 5.6
Intensity Change (Log2) – – – -0.02 0.3 0.14 0.12 0.24 0.3 0.38 0.34 0.44 0.26 0.36 0.4 1.0 0.96 0.95 0.82 0.55 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -1.1 -2.2 -3.1 -3.9 -4.2 -4.0 -4.1 -4.3 -1.3 -1.4 -1.3 -1.1 -1.9 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.6 0 3.1 0 3.3 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.9 2.7 2.8 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6363 9.4432 9.8378 9.3791 8.9195 9.5801 7.3921 8.6469 7.3056 6.0233 5.9766 6.8051 7.6683 9.0742 8.5351 8.7652 7.2197 7.8362
Actin 0.0469 0.0005 0.0151 0.0047 0.0512 0.0029 0.0035 0.0008 0.0237 0.0063 0.0011 0.0004 0.0005 0.0003 0.0019 0.0001 0.0003 0.0003
Bud 0.0004 0.0004 0.0003 0.0003 0.0003 0.0003 0.0002 0.0001 0.0004 0.0001 0 0 0 0.0001 0.0006 0.0001 0.0001 0.0001
Bud Neck 0.0023 0.0002 0.0007 0.0016 0.0014 0.0014 0.0003 0.0002 0.0003 0.002 0.0004 0.0008 0.0001 0.0001 0.0003 0.0002 0.0004 0.0013
Bud Periphery 0.0005 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0 0.0002 0.0001 0 0 0 0 0.0003 0 0 0.0001
Bud Site 0.0019 0.0065 0.0012 0.0007 0.0013 0.0001 0.0012 0.0002 0.0005 0.0005 0.0001 0 0.0001 0.0003 0.0014 0 0 0
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0 0 0 0 0.0001 0 0 0
Cytoplasm 0.7964 0.9622 0.9145 0.8886 0.8375 0.9353 0.7887 0.9523 0.9202 0.9159 0.9271 0.9729 0.9627 0.9924 0.9715 0.9883 0.9928 0.9893
Cytoplasmic Foci 0.0222 0.0017 0.0076 0.0045 0.0116 0.0018 0.0269 0.0125 0.0025 0.0127 0.004 0.0016 0.0093 0.0007 0.0021 0.0008 0.0007 0.0014
Eisosomes 0.0003 0 0.0001 0.0001 0.0002 0 0.0005 0 0.0002 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0112 0.0064 0.0097 0.0061 0.0063 0.0164 0.0069 0.0005 0.0045 0.0041 0.0028 0.0027 0.0057 0.0013 0.0016 0.0026 0.0014 0.0019
Endosome 0.0182 0.0007 0.0109 0.0101 0.0243 0.0027 0.0039 0.0003 0.0037 0.0038 0.003 0.0005 0.0018 0.0001 0.0004 0.0005 0.0002 0.0004
Golgi 0.004 0.0001 0.0029 0.0047 0.0074 0.0039 0.003 0.001 0.0024 0.0011 0.0002 0.0001 0.0002 0 0.0001 0 0 0
Lipid Particles 0.0043 0 0.0057 0.0058 0.011 0.0006 0.01 0.0007 0.0009 0.0035 0.0006 0 0.0002 0 0.0001 0 0 0
Mitochondria 0.0014 0.0001 0.0019 0.0216 0.0061 0.0049 0.0018 0.0001 0.002 0.0004 0.0001 0 0 0 0.0001 0 0 0
None 0.0269 0.016 0.0076 0.0023 0.0044 0.0161 0.0929 0.0015 0.0317 0.0373 0.0533 0.0191 0.0147 0.0028 0.0158 0.0026 0.0016 0.0016
Nuclear Periphery 0.0216 0.0013 0.0067 0.0188 0.0081 0.0028 0.0014 0.0002 0.0024 0.001 0.0019 0.0003 0.0015 0.0003 0.0006 0.001 0.0005 0.0005
Nucleolus 0.001 0 0.0001 0.0004 0.0005 0 0.0206 0 0.0002 0.0005 0.0001 0 0 0 0 0 0 0
Nucleus 0.0227 0.0027 0.0038 0.0155 0.0063 0.0083 0.009 0.0008 0.0017 0.0014 0.0019 0.0012 0.002 0.0011 0.0023 0.0031 0.0017 0.0025
Peroxisomes 0.0056 0 0.0074 0.0076 0.0113 0.0003 0.0255 0.0286 0.0003 0.0024 0.0001 0 0.0002 0 0.0001 0 0 0
Punctate Nuclear 0.0092 0.0004 0.0017 0.0035 0.0083 0.0003 0.0025 0.0002 0.0014 0.0062 0.0028 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0002
Vacuole 0.0018 0.0006 0.0012 0.0019 0.0012 0.0014 0.0004 0 0.0003 0.0004 0.0003 0.0002 0.0003 0.0001 0.0004 0.0004 0.0001 0.0002
Vacuole Periphery 0.001 0.0001 0.0006 0.0008 0.0008 0.0003 0.0003 0 0.0003 0.0001 0.0001 0 0 0 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.7969 55.5392 115.3949 77.6974 90.2188 139.0911 61.2096 62.9161 102.0862 60.3801
Translational Efficiency 0.6766 0.7942 0.4294 1.5352 0.9935 0.3332 0.7472 0.9951 0.6126 0.8193

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘