Standard name
Description Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.10 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.88 0.76 0.82 0.84 0.83 0.75 0.68 0.62 0.51 0.66 0.69 0.65 0.57 0.74 0.79 0.67 0.57 0.63 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.49 0.32 0.53 0.34 0.20 0.32 0.35 0.68 0.62 0.74 0.66 0.50 0.53 0.34 0.22 0.16 0.24 0.35 0.25 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 0 1 3 2 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 0 2 5 1 10 0 3 0 2 0 3 14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Endosome 0 0 1 1 1 0 0 0 0 0 2 0 0 2 1 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0
Mitochondria 0 1 0 0 1 1 2 0 0 10 4 0 0 0 0 0 0 0 0 0
Nucleus 131 136 39 116 168 199 200 62 81 49 105 11 51 73 112 128 54 12 31 60
Nuclear Periphery 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 73 50 27 48 41 78 93 62 80 72 104 8 42 43 32 26 19 7 12 22
Peroxisomes 0 0 0 1 0 1 1 0 0 0 0 0 0 3 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 1 1 0 0 1 3 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 3 4 0 3 0 1 1 0 1 0 3 2 3 3 0 0 2
Unique Cell Count 149 155 51 142 201 241 268 91 130 97 158 16 79 127 152 162 81 22 50 91
Labelled Cell Count 205 190 67 171 222 282 311 124 169 137 220 20 98 144 152 162 81 22 50 91

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.9 23.6 15.4 16.8 16.8 14.9 14.3 11.7 11.6 10.4 11.9 18.0 17.2 17.7 10.9 13.7
Std Deviation (1e-4) 4.7 5.7 4.5 4.4 4.7 3.9 4.1 4.9 3.3 2.9 4.1 6.3 5.8 6.0 4.3 4.3
Intensity Change (Log2) 0.1 0.1 -0.0 -0.1 -0.4 -0.4 -0.6 -0.4 0.2 0.2 0.2 -0.5 -0.2

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0 2.2 0.6 0.3 -1.1 -2.1 -0.2 -1.1 -0.9 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.4 -4.7 -2.8 -2.5 1.8 1.1 2.6 1.7 -0.2 0 -2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0