TheCellVision

RSC8

Standard name	RSC8 ORF YFR037C Aliases SWH3
Human Ortholog	SMARCC2 SMARCC1
Description	Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

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Scale images

rsc8-ts21
rsc8-ts16

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0.05	0	0
Bud Neck	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Cytoplasm	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0.11	–	–	–	0	0	0	0	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0	0	–	0	0	0.1	0	0	0	0	–	–	–	0	0	0	0	0	0
Nucleus	0.88	0.88	0.76	0.82	0.84	0.83	0.75	–	0.68	0.62	0.51	0.66	0.69	0.65	0.57	–	–	–	0.74	0.79	0.67	0.57	0.63	0.67
Nuclear Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Nucleolus	0.49	0.32	0.53	0.34	0.2	0.32	0.35	–	0.68	0.62	0.74	0.66	0.5	0.53	0.34	–	–	–	0.22	0.16	0.24	0.35	0.25	0.25
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0	0	–	0	0	0	0	0.06	0	0	–	–	–	0	0	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Bud	0	0	0	0	0	1	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	–	0	0	1	0	0	0	1	–	–	–	0	0	0	0	0	0
Bud Site	0	0	0	0	0	1	1	–	0	1	3	2	0	1	0	–	–	–	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	0	0	0	0
Cytoplasm	1	2	0	2	5	1	10	–	0	3	0	2	0	3	14	–	–	–	0	0	0	0	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–	0	0	1	0	0	0
Endosome	0	0	1	1	1	0	0	–	0	0	0	2	0	0	2	–	–	–	1	1	0	0	0	1
Golgi	0	0	0	0	0	0	0	–	0	1	0	0	0	0	2	–	–	–	0	0	0	0	0	0
Mitochondria	0	1	0	0	1	1	2	–	0	0	10	4	0	0	0	–	–	–	0	0	0	0	0	0
Nucleus	131	136	39	116	168	199	200	–	62	81	49	105	11	51	73	–	–	–	112	128	54	12	31	60
Nuclear Periphery	0	0	0	0	1	0	1	–	0	1	0	1	0	0	0	–	–	–	0	0	0	0	0	0
Nucleolus	73	50	27	48	41	78	93	–	62	80	72	104	8	42	43	–	–	–	32	26	19	7	12	22
Peroxisomes	0	0	0	1	0	1	1	–	0	0	0	0	0	0	3	–	–	–	0	0	0	0	0	0
SpindlePole	0	0	0	0	1	0	0	–	0	1	1	0	0	1	3	–	–	–	0	0	0	0	0	0
Vac/Vac Membrane	0	1	0	3	4	0	3	–	0	1	1	0	1	0	3	–	–	–	2	3	3	0	0	2
Unique Cell Count	149	155	51	142	201	241	268		91	130	97	158	16	79	127				152	162	81	22	50	91
Labelled Cell Count	205	190	67	171	222	282	311		124	169	137	220	20	98	144				152	162	81	22	50	91

Yeast GFP Assignment

Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	16.9	23.6	15.4	16.8	16.8	14.9	14.3	–	11.7	11.6	10.4	11.9	18.0	17.2	17.7	–	–	–	–	10.9	13.7
Std Deviation (1e^-4)	4.7	5.7	4.5	4.4	4.7	3.9	4.1	–	4.9	3.3	2.9	4.1	6.3	5.8	6.0	–	–	–	–	4.3	4.3
Intensity Change (Log₂)	–	–	–	0.13	0.12	-0.05	-0.11	–	-0.4	-0.41	-0.57	-0.37	0.22	0.16	0.2	–	–	–	–	-0.5	-0.18

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Bud	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Bud Neck	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Bud Site	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Cell Periphery	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Cytoplasm	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Endoplasmic Reticulum	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Endosome	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Golgi	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Mitochondria	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Nucleus	0.8	1.2	1.0	-0.3	–	-1.1	-1.8	-3.1	-1.3	-0.6	-1.4	-2.4	–	–	–
Nuclear Periphery	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Nucleolus	-2.4	-4.7	-2.8	-2.5	–	1.8	1.1	2.6	1.7	-0.2	0	-2.4	–	–	–
Peroxisomes	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
SpindlePole	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–
Vacuole	0	0	0	0	–	0	0	0	0	0	0	0	–	–	–

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	15.543	14.5381	13.5536	13.0076	14.1809	13.7022	15.6357	15.0166	14.6133	13.3777	13.3496	14.1408	14.5158	14.3423	13.5206	11.9967	11.489	13.5823
Actin	0.0107	0	0.0065	0	0	0	0.0101	0	0.0137	0.0108	0.0078	0.0013	0.0009	0	0.0078	0	0.0339	0.0042
Bud	0.0004	0	0.0002	0	0.0001	0	0.0003	0	0.0001	0.0003	0.0002	0.0001	0.0002	0	0.0002	0.0001	0.0008	0.0001
Bud Neck	0.0001	0	0.0001	0	0.0002	0.0002	0.0001	0	0.0001	0	0.0003	0.0002	0.0001	0	0	0	0.0006	0.0002
Bud Periphery	0.0004	0	0.0003	0	0.0001	0.0001	0.0002	0	0.0002	0.0004	0.0003	0.0001	0.0003	0	0.0003	0.0001	0.0009	0.0004
Bud Site	0.0017	0	0.0006	0.0001	0.0001	0.0001	0.0008	0	0.0004	0.0008	0.0004	0.0001	0.0004	0	0.0006	0	0.0016	0.0001
Cell Periphery	0.0001	0	0.0002	0.0001	0.0001	0.0001	0	0	0	0	0.0002	0.0001	0.0001	0	0	0.0001	0.0001	0.0001
Cytoplasm	0.001	0	0.0004	0.0001	0	0.0001	0.0033	0	0.0012	0.0003	0.0018	0.0002	0.0007	0	0.001	0	0.0024	0
Cytoplasmic Foci	0.0194	0	0.0033	0.0043	0	0.0065	0.0064	0	0.0016	0.0138	0.0032	0.0024	0.0063	0	0.0005	0	0.01	0.0004
Eisosomes	0.0001	0	0.0001	0	0	0	0.0003	0	0.0001	0	0	0	0	0	0.0001	0	0.0001	0.0001
Endoplasmic Reticulum	0.0004	0	0.0001	0	0	0	0.0014	0	0.0008	0.0002	0.0023	0.0009	0.0001	0	0.0001	0	0.0059	0.0002
Endosome	0.0063	0	0.0006	0.0001	0	0.0002	0.0047	0	0.0022	0.0051	0.0209	0.0102	0.0005	0	0.0007	0	0.0565	0.0022
Golgi	0.0015	0	0.001	0.0001	0	0.0001	0.001	0	0.001	0.0046	0.0028	0.0031	0.0001	0	0.0014	0	0.0277	0.0023
Lipid Particles	0.0053	0	0.0019	0.0082	0.0001	0.0011	0.0045	0	0.0016	0.0097	0.0045	0.0011	0.0008	0	0.0004	0	0.0095	0.0041
Mitochondria	0.0021	0.0002	0.0212	0.0041	0.0025	0.0002	0.0013	0.0002	0.0016	0.0025	0.0064	0.0041	0.0053	0.0004	0.0097	0.0007	0.0132	0.0121
None	0.0013	0	0.001	0.0003	0	0.0001	0.0194	0	0.0006	0.0006	0.0011	0.0001	0.0009	0	0.0007	0	0.0007	0.0002
Nuclear Periphery	0.0086	0.0055	0.0011	0.0005	0.0002	0.0002	0.0115	0.0001	0.014	0.0011	0.0596	0.0023	0.0056	0	0.0013	0.0001	0.0203	0.0014
Nucleolus	0.1038	0.0568	0.0527	0.0959	0.5317	0.2599	0.0788	0.0534	0.0758	0.0263	0.552	0.2059	0.1104	0.0751	0.0423	0.0729	0.3774	0.184
Nucleus	0.8131	0.9291	0.8993	0.8821	0.4632	0.7255	0.8246	0.9462	0.8632	0.8904	0.3175	0.7539	0.8269	0.9243	0.9266	0.9251	0.4253	0.7858
Peroxisomes	0.0116	0	0.0058	0.0003	0	0	0.0037	0	0.006	0.0262	0.0015	0.0009	0.0002	0	0.0008	0	0.0034	0.0009
Punctate Nuclear	0.0112	0.008	0.0028	0.001	0.0002	0.0054	0.0268	0.0001	0.0152	0.0064	0.014	0.0114	0.0392	0	0.0054	0	0.0065	0.0003
Vacuole	0.0004	0	0.0004	0.0005	0.0004	0.0002	0.0003	0	0.0002	0.0001	0.001	0.0005	0.0004	0	0.0001	0.0004	0.0013	0.0002
Vacuole Periphery	0.0005	0.0002	0.0005	0.002	0.0011	0.0001	0.0004	0	0.0003	0.0002	0.0021	0.0012	0.0006	0	0.0001	0.0004	0.0019	0.0006

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	42.5147	52.4417	54.2173	55.8131	64.5228	54.0198	58.8326	63.1307	67.7965	58.7326
Translational Efficiency	2.5074	2.0732	1.7691	1.8975	1.756	1.7888	1.6175	1.4715	1.3097	1.5586

Hit Data

Dataset	Hit
Protein Concentration	✔
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
44	93	1891	14	113	1724	2123	36	157	1817	4014	50

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	1112.59	1486.45	1763.05	2012.55	1403.24	1352.24	1717.17	2095.08	1321.78	1359.11	1738.78	2071.97
Standard Deviation	292.49	348.68	376.69	515.68	294.72	305.90	376.19	496.46	321.76	309.65	377.12	503.28
Intensity Change Log ₂	—	0.417949	0.664151	0.855103	—	-0.053411	0.291271	0.578244	—	0.174191	0.468144	0.707272

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000953	0.000588	0.000798	0.001088	0.000635	0.000807	0.000803	0.001261	0.000724	0.000796	0.000800	0.001212
Bud Neck	0.006760	0.002683	0.009406	0.010780	0.003735	0.005748	0.013036	0.028961	0.004583	0.005591	0.011326	0.023870
Bud Site	0.000558	0.000475	0.000895	0.000878	0.000389	0.000967	0.001435	0.004980	0.000436	0.000942	0.001181	0.003831
Cell Periphery	0.000022	0.000029	0.000026	0.000068	0.000017	0.000078	0.000198	0.000238	0.000019	0.000075	0.000117	0.000190
Cytoplasm	0.000044	0.005774	0.000035	0.000700	0.000126	0.000924	0.000343	0.000639	0.000103	0.001172	0.000198	0.000656
Cytoplasmic Foci	0.000055	0.000951	0.000192	0.000170	0.000639	0.001097	0.000752	0.001695	0.000475	0.001089	0.000488	0.001268
Eisosomes	0.000050	0.000096	0.000161	0.000103	0.000097	0.000140	0.000146	0.000162	0.000084	0.000137	0.000153	0.000145
Endoplasmic Reticulum	0.000777	0.000321	0.000225	0.000498	0.000367	0.000697	0.000457	0.000435	0.000482	0.000677	0.000348	0.000453
Endosome	0.000026	0.000078	0.000008	0.000030	0.000012	0.000228	0.000253	0.000106	0.000016	0.000220	0.000138	0.000085
Golgi	0.000091	0.000079	0.000072	0.000129	0.000079	0.000146	0.000206	0.000281	0.000082	0.000143	0.000143	0.000238
Lipid Particles	0.000012	0.000037	0.000004	0.000036	0.000012	0.000273	0.000293	0.000039	0.000012	0.000261	0.000157	0.000038
Mitochondria	0.002051	0.001936	0.001967	0.003172	0.001406	0.001562	0.002044	0.004034	0.001587	0.001581	0.002008	0.003793
Mitotic Spindle	0.000224	0.004249	0.000600	0.000445	0.002389	0.004341	0.002596	0.003276	0.001782	0.004336	0.001656	0.002483
None	0.000817	0.000732	0.000675	0.001795	0.000707	0.001456	0.001253	0.001374	0.000738	0.001419	0.000981	0.001492
Nuclear Periphery	0.000233	0.000085	0.000051	0.000351	0.000103	0.000115	0.000282	0.000218	0.000140	0.000114	0.000173	0.000255
Nuclear Periphery Foci	0.000034	0.000073	0.000014	0.000029	0.000036	0.000457	0.000251	0.000042	0.000036	0.000437	0.000139	0.000038
Nucleolus	0.103379	0.162153	0.110970	0.045583	0.141117	0.117094	0.072326	0.136914	0.130541	0.119400	0.090531	0.111341
Nucleus	0.878537*	0.806315*	0.863603*	0.930226*	0.827119*	0.858660*	0.896956*	0.783716*	0.841529*	0.855981*	0.881243*	0.824739*
Peroxisomes	0.004892	0.012760	0.009877	0.002469	0.018825	0.004044	0.004445	0.029870	0.014921	0.004490	0.007004	0.022198
Vacuole	0.000189	0.000215	0.000137	0.001218	0.000532	0.000576	0.001047	0.001034	0.000436	0.000557	0.000619	0.001086
Vacuole Periphery	0.000298	0.000372	0.000282	0.000234	0.001657	0.000591	0.000878	0.000726	0.001276	0.000580	0.000597	0.000588

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	NaN	NaN	NaN	NaN	NaN	-1.42	-4.03	-3.49	-2.22	-2.43	-0.55	-1.99	-3.47	-2.83	-3.06
Bud Neck	NaN	NaN	NaN	NaN	NaN	-2.14	-10.37	-1.55	-1.45	-0.98	-1.08	-9.76	-1.82	-1.76	-1.26
Bud Site	NaN	NaN	NaN	NaN	NaN	-2.85	-5.99	-1.65	-1.42	-1.25	-2.87	-6.39	-1.82	-1.52	-1.43
Cell Periphery	NaN	NaN	NaN	NaN	NaN	-3.56	-2.75	-1.50	-1.05	-0.30	-3.65	-2.79	-1.63	-1.04	-0.72
Cytoplasm	NaN	NaN	NaN	NaN	NaN	-2.17	-0.76	-0.93	1.31	-0.64	-2.41	-0.25	-1.56	1.59	-1.53
Cytoplasmic Foci	NaN	NaN	NaN	NaN	NaN	-1.29	-0.59	-1.64	-0.76	-1.40	-1.75	-0.38	-1.65	-0.25	-1.58
Eisosomes	NaN	NaN	NaN	NaN	NaN	-2.97	-4.37	-1.74	-0.78	-0.61	-4.27	-8.59	-2.05	-0.44	0.13
Endoplasmic Reticulum	NaN	NaN	NaN	NaN	NaN	-3.20	-2.08	-0.70	1.89	0.34	-1.81	2.40	-1.14	-0.42	-1.65
Endosome	NaN	NaN	NaN	NaN	NaN	-2.75	-2.79	-1.23	1.29	1.20	-2.75	-2.62	-1.62	1.36	0.30
Golgi	NaN	NaN	NaN	NaN	NaN	-1.80	-1.63	-2.80	-1.86	-1.16	-1.83	-1.36	-3.32	-2.10	-2.25
Lipid Particles	NaN	NaN	NaN	NaN	NaN	-1.94	-1.24	-1.57	1.74	1.10	-1.95	-1.20	-1.85	1.31	0.42
Mitochondria	NaN	NaN	NaN	NaN	NaN	-0.29	-3.09	-3.59	-3.38	-2.73	0.19	-2.29	-4.07	-4.03	-3.44
Mitotic Spindle	NaN	NaN	NaN	NaN	NaN	-0.42	0.50	-0.05	0.35	-0.56	-1.02	0.71	-0.11	0.89	-0.84
None	NaN	NaN	NaN	NaN	NaN	-1.03	-0.88	-0.93	0.33	-0.33	-1.12	-0.26	-1.40	0.19	-1.56
Nuclear Periphery	NaN	NaN	NaN	NaN	NaN	0.31	-1.32	-0.82	-1.62	0.58	0.72	-0.07	-1.59	-2.41	-1.79
Nuclear Periphery Foci	NaN	NaN	NaN	NaN	NaN	-1.94	-1.03	0.63	2.10	1.31	-2.01	-0.76	0.18	2.05	0.90
Nucleolus	NaN	NaN	NaN	NaN	NaN	1.65	4.17	-0.41	-1.36	-2.73	1.09	3.03	0.23	-0.37	-1.48
Nucleus	NaN	NaN	NaN	NaN	NaN	-1.67	-3.38	1.47	2.37	3.14	-1.05	-2.46	1.15	1.76	2.42
Peroxisomes	NaN	NaN	NaN	NaN	NaN	2.03	1.90	-1.30	-2.77	-2.69	1.97	1.34	-1.19	-2.57	-2.19
Vacuole	NaN	NaN	NaN	NaN	NaN	-0.39	-1.02	-0.94	-0.80	-0.13	-0.75	-0.59	-1.59	-1.47	-1.49
Vacuole Periphery	NaN	NaN	NaN	NaN	NaN	0.70	0.53	0.55	-0.64	0.06	0.63	0.64	0.52	-0.42	-0.54