Standard name | |
---|---|
Human Ortholog | |
Description | Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 7 | 5 |
Bud | 0 | 4 | 0 | 1 | 1 | 3 | 5 | 9 | 7 | 8 | 5 | 4 | 1 | 0 | 0 | 3 | 3 | 5 | 3 | 0 | 1 | 0 | 10 | 10 |
Bud Neck | 1 | 2 | 2 | 14 | 28 | 13 | 20 | 32 | 13 | 30 | 3 | 10 | 8 | 4 | 2 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 9 | 4 | 9 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 2 | 4 | 3 | 2 | 2 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 7 |
Cytoplasm | 161 | 112 | 78 | 282 | 451 | 471 | 549 | 556 | 411 | 411 | 247 | 307 | 362 | 514 | 565 | 61 | 34 | 45 | 131 | 95 | 65 | 188 | 383 | 462 |
Endoplasmic Reticulum | 1 | 3 | 2 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 14 | 7 | 14 | 3 | 0 | 0 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 8 | 124 | 52 | 112 | 8 | 2 | 2 | 2 | 4 | 6 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 1 |
Mitochondria | 25 | 17 | 0 | 1 | 4 | 29 | 74 | 45 | 79 | 55 | 123 | 64 | 0 | 0 | 0 | 10 | 9 | 20 | 27 | 8 | 13 | 24 | 28 | 29 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 3 | 10 | 9 | 7 | 6 | 4 | 3 | 1 | 0 | 0 | 13 | 10 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 26 | 16 | 25 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 10 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | 4 | 7 | 13 | 20 | 29 | 31 | 10 | 20 | 17 | 12 | 9 | 13 | 17 | 170 | 94 | 163 | 9 | 6 | 11 | 13 | 27 | 26 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 5 | 4 | 5 | 6 | 6 | 0 | 4 | 6 | 0 | 1 | 0 | 42 | 20 | 33 | 4 | 5 | 2 | 1 | 1 | 6 |
Unique Cell Count | 180 | 126 | 86 | 295 | 481 | 517 | 643 | 636 | 476 | 479 | 342 | 369 | 377 | 530 | 589 | 330 | 178 | 318 | 200 | 129 | 104 | 243 | 489 | 588 |
Labelled Cell Count | 192 | 142 | 89 | 310 | 511 | 552 | 699 | 694 | 535 | 536 | 410 | 407 | 385 | 535 | 595 | 482 | 254 | 454 | 200 | 129 | 104 | 243 | 489 | 588 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 3.0 | 4.6 | 4.3 | 4.7 | 3.9 | 3.5 | 4.0 | 3.5 | 3.4 | 3.3 | 3.5 | 4.8 | 5.0 | 5.1 | 5.9 | 6.1 | 5.9 | 4.4 | 4.6 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.7 | 1.6 | 1.9 | 2.2 | 1.5 | 1.8 | 1.6 | 1.1 | 1.4 | 1.2 | 1.2 | 1.4 | 1.9 | 1.2 | 1.8 | 1.6 | 1.1 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.04 | -0.24 | -0.39 | -0.18 | -0.39 | -0.42 | -0.48 | -0.4 | 0.06 | 0.13 | 0.16 | 0.37 | 0.41 | 0.37 | -0.06 | 0.0 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 3.2 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7794 | 0.4488 | 0.2998 | -0.017 | 1.0126 | 0.5287 | -1.3508 | -0.5551 | -0.4419 | -1.1014 | -0.7599 | -0.2955 | -1.0883 | -1.598 | -1.3778 | -1.6709 | -2.1948 | -1.8974 |
Actin | 0.0296 | 0.0023 | 0.0173 | 0.0009 | 0.0355 | 0.0021 | 0.0271 | 0.001 | 0.0128 | 0.032 | 0.0112 | 0.0074 | 0.0628 | 0.0012 | 0.0132 | 0.0028 | 0.0002 | 0.0014 |
Bud | 0.0024 | 0.0003 | 0.0007 | 0.0014 | 0.0013 | 0.0006 | 0.0016 | 0.0009 | 0.0006 | 0.0002 | 0.008 | 0.0006 | 0.0006 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 |
Bud Neck | 0.002 | 0.0002 | 0.0007 | 0.0053 | 0.001 | 0.0071 | 0.0023 | 0.0003 | 0.0009 | 0.0087 | 0.0007 | 0.0035 | 0.003 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0006 |
Bud Periphery | 0.0057 | 0.0003 | 0.0015 | 0.0033 | 0.0026 | 0.0033 | 0.0047 | 0.0021 | 0.0008 | 0.0005 | 0.0293 | 0.002 | 0.0007 | 0.0001 | 0.0005 | 0 | 0.0003 | 0 |
Bud Site | 0.0097 | 0.0021 | 0.0027 | 0.0185 | 0.003 | 0.0015 | 0.0102 | 0.0042 | 0.0024 | 0.0015 | 0.0023 | 0.0005 | 0.0096 | 0.0012 | 0.0027 | 0 | 0.0003 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 |
Cytoplasm | 0.0327 | 0.0498 | 0.0384 | 0.0438 | 0.0126 | 0.0485 | 0.0528 | 0.0466 | 0.0331 | 0.0867 | 0.0302 | 0.0641 | 0.0312 | 0.1109 | 0.0748 | 0.0111 | 0.1284 | 0.1592 |
Cytoplasmic Foci | 0.0802 | 0.0548 | 0.0658 | 0.0977 | 0.0987 | 0.0652 | 0.1116 | 0.0709 | 0.1027 | 0.0868 | 0.0637 | 0.0602 | 0.1699 | 0.1167 | 0.0818 | 0.0639 | 0.0544 | 0.0739 |
Eisosomes | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0062 | 0.0007 | 0.0012 | 0.0006 | 0.0044 | 0.0025 | 0.0016 | 0.0012 | 0.0013 | 0.0011 | 0.0012 | 0.0018 | 0.0037 | 0.0066 | 0.0102 | 0.0003 | 0.0111 | 0.0009 |
Endosome | 0.0216 | 0.0074 | 0.0064 | 0.0059 | 0.0597 | 0.0062 | 0.0191 | 0.0051 | 0.0142 | 0.0191 | 0.0549 | 0.0282 | 0.0441 | 0.0152 | 0.0195 | 0.0018 | 0.0577 | 0.0047 |
Golgi | 0.0067 | 0.0007 | 0.0035 | 0.002 | 0.0203 | 0.001 | 0.0034 | 0.0022 | 0.009 | 0.007 | 0.0549 | 0.011 | 0.0176 | 0.0012 | 0.0047 | 0.0007 | 0.0011 | 0.0004 |
Lipid Particles | 0.0227 | 0.0048 | 0.0073 | 0.0143 | 0.07 | 0.0049 | 0.0098 | 0.0032 | 0.0073 | 0.0164 | 0.054 | 0.0034 | 0.0553 | 0.0131 | 0.0097 | 0.0061 | 0.0106 | 0.0009 |
Mitochondria | 0.0141 | 0.0003 | 0.0015 | 0.0026 | 0.0246 | 0.0011 | 0.002 | 0.0009 | 0.0052 | 0.0019 | 0.0341 | 0.0027 | 0.0038 | 0.0004 | 0.0168 | 0.0005 | 0.0011 | 0.0001 |
None | 0.6277 | 0.8166 | 0.7421 | 0.6978 | 0.5861 | 0.7239 | 0.6616 | 0.7952 | 0.6945 | 0.6538 | 0.6122 | 0.7116 | 0.4334 | 0.6139 | 0.7076 | 0.7686 | 0.7061 | 0.6178 |
Nuclear Periphery | 0.0243 | 0.0017 | 0.0015 | 0.0008 | 0.0112 | 0.0039 | 0.0023 | 0.0015 | 0.0016 | 0.0026 | 0.002 | 0.0023 | 0.0064 | 0.0042 | 0.0046 | 0.0008 | 0.0057 | 0.0021 |
Nucleolus | 0.0024 | 0.0007 | 0.0014 | 0.0027 | 0.0016 | 0.0011 | 0.0017 | 0.0006 | 0.0021 | 0.0006 | 0.0005 | 0.0051 | 0.0037 | 0.0008 | 0.0005 | 0.0009 | 0.0028 | 0.0004 |
Nucleus | 0.0097 | 0.0036 | 0.0081 | 0.0015 | 0.0021 | 0.004 | 0.0063 | 0.0016 | 0.006 | 0.0014 | 0.0005 | 0.0184 | 0.0047 | 0.0022 | 0.0016 | 0.001 | 0.0036 | 0.0085 |
Peroxisomes | 0.015 | 0.0055 | 0.011 | 0.0284 | 0.0322 | 0.0044 | 0.0039 | 0.0013 | 0.0156 | 0.0061 | 0.0329 | 0.0031 | 0.0381 | 0.0022 | 0.0146 | 0.0069 | 0.0011 | 0.0027 |
Punctate Nuclear | 0.0817 | 0.0476 | 0.0876 | 0.0705 | 0.0264 | 0.1167 | 0.0758 | 0.0598 | 0.0886 | 0.0714 | 0.0042 | 0.0689 | 0.1063 | 0.108 | 0.0319 | 0.1328 | 0.0059 | 0.1254 |
Vacuole | 0.0023 | 0.0004 | 0.0006 | 0.0013 | 0.0031 | 0.0012 | 0.0013 | 0.0008 | 0.0007 | 0.0006 | 0.0016 | 0.0038 | 0.0029 | 0.0012 | 0.0029 | 0.0002 | 0.0066 | 0.0005 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0001 | 0.0002 | 0.0025 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.001 | 0.0011 | 0.0008 | 0.0003 | 0.0013 | 0.0001 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.1923 | 7.2661 | 6.0657 | 7.7132 | 12.5146 | 7.1082 | 8.9539 | 5.5784 | 7.1179 | 10.5333 |
Translational Efficiency | 1.0558 | 0.8532 | 1.0488 | 0.8243 | 0.8062 | 2.2053 | 0.7629 | 0.9702 | 1.0015 | 1.0447 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
86 | 154 | 2503 | 1537 | 1466 | 2081 | 303 | 1182 | 1552 | 2235 | 2806 | 2719 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 633.77 | 748.23 | 738.26 | 757.70 | 607.48 | 695.53 | 841.57 | 793.04 | 608.94 | 699.16 | 749.42 | 773.06 |
Standard Deviation | 91.14 | 83.72 | 120.15 | 118.23 | 77.60 | 93.25 | 100.47 | 108.80 | 78.64 | 93.58 | 122.46 | 115.56 |
Intensity Change Log 2 | — | 0.239522 | 0.220170 | 0.257667 | — | 0.195276 | 0.470246 | 0.384557 | — | 0.218037 | 0.347976 | 0.321163 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001775 | 0.001233 | 0.000350 | 0.000640 | 0.000101 | 0.000451 | 0.000384 | 0.001185 | 0.000194 | 0.000505 | 0.000354 | 0.000877 |
Bud Neck | 0.051281 | 0.045981 | 0.014611 | 0.011958 | 0.015147 | 0.034977 | 0.001889 | 0.010832 | 0.017150 | 0.035735 | 0.013237 | 0.011468 |
Bud Site | 0.045758 | 0.022972 | 0.020985 | 0.044726 | 0.003480 | 0.018840 | 0.010843 | 0.071169 | 0.005823 | 0.019125 | 0.019889 | 0.056221 |
Cell Periphery | 0.000545 | 0.000164 | 0.000181 | 0.000254 | 0.000107 | 0.000239 | 0.000182 | 0.000235 | 0.000131 | 0.000234 | 0.000181 | 0.000246 |
Cytoplasm | 0.277473* | 0.193007* | 0.337043* | 0.435691* | 0.465399* | 0.382924* | 0.244238* | 0.459971* | 0.454986* | 0.369838* | 0.327022* | 0.446246* |
Cytoplasmic Foci | 0.222881* | 0.385356* | 0.008121 | 0.023085 | 0.249470* | 0.200963* | 0.018678 | 0.023781 | 0.247997* | 0.213668* | 0.009261 | 0.023388 |
Eisosomes | 0.000269 | 0.000242 | 0.000027 | 0.000052 | 0.000125 | 0.000146 | 0.000086 | 0.000053 | 0.000133 | 0.000153 | 0.000034 | 0.000052 |
Endoplasmic Reticulum | 0.002065 | 0.001266 | 0.001666 | 0.002371 | 0.000789 | 0.001129 | 0.004491 | 0.001727 | 0.000859 | 0.001138 | 0.001971 | 0.002091 |
Endosome | 0.020553 | 0.060461 | 0.001820 | 0.008052 | 0.010259 | 0.020569 | 0.003349 | 0.008338 | 0.010829 | 0.023318 | 0.001985 | 0.008176 |
Golgi | 0.005412 | 0.014595 | 0.000159 | 0.002878 | 0.002133 | 0.005654 | 0.000096 | 0.007099 | 0.002315 | 0.006270 | 0.000152 | 0.004713 |
Lipid Particles | 0.016571 | 0.008458 | 0.000845 | 0.001802 | 0.012082 | 0.012452 | 0.003622 | 0.001120 | 0.012331 | 0.012177 | 0.001145 | 0.001506 |
Mitochondria | 0.009572 | 0.027776 | 0.001752 | 0.001704 | 0.004357 | 0.018061 | 0.000505 | 0.007678 | 0.004646 | 0.018730 | 0.001617 | 0.004301 |
Mitotic Spindle | 0.022387 | 0.013577 | 0.016301 | 0.053131 | 0.001722 | 0.022566 | 0.004255 | 0.045275 | 0.002867 | 0.021947 | 0.015000 | 0.049716 |
None | 0.008714 | 0.000657 | 0.005443 | 0.010668 | 0.004778 | 0.004621 | 0.011471 | 0.009992 | 0.004996 | 0.004348 | 0.006094 | 0.010374 |
Nuclear Periphery | 0.000619 | 0.000453 | 0.002171 | 0.002144 | 0.000632 | 0.001044 | 0.002181 | 0.001229 | 0.000631 | 0.001003 | 0.002172 | 0.001746 |
Nuclear Periphery Foci | 0.001447 | 0.000957 | 0.000417 | 0.001892 | 0.000590 | 0.000414 | 0.002109 | 0.000858 | 0.000637 | 0.000452 | 0.000600 | 0.001443 |
Nucleolus | 0.008796 | 0.001588 | 0.001925 | 0.001069 | 0.001857 | 0.002938 | 0.002096 | 0.000564 | 0.002242 | 0.002845 | 0.001943 | 0.000850 |
Nucleus | 0.222949* | 0.077264 | 0.562729* | 0.290445* | 0.185221* | 0.182227* | 0.629745* | 0.237844* | 0.187312* | 0.174995* | 0.569965* | 0.267578* |
Peroxisomes | 0.017920 | 0.066545 | 0.000782 | 0.001619 | 0.007219 | 0.015908 | 0.000483 | 0.002409 | 0.007812 | 0.019397 | 0.000749 | 0.001963 |
Vacuole | 0.059442 | 0.074380 | 0.021913 | 0.098967 | 0.033651 | 0.070842 | 0.059129 | 0.098643 | 0.035081 | 0.071086 | 0.025932 | 0.098826 |
Vacuole Periphery | 0.003571 | 0.003065 | 0.000758 | 0.006852 | 0.000880 | 0.003034 | 0.000167 | 0.009996 | 0.001029 | 0.003036 | 0.000694 | 0.008219 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.56 | 1.43 | 1.33 | 1.60 | -0.89 | -4.77 | -6.27 | -4.72 | -1.82 | -0.17 | -3.49 | -4.43 | -5.05 | -1.24 | -1.53 |
Bud Neck | 0.20 | 2.22 | 2.71 | 4.63 | 4.76 | -8.78 | 6.84 | 3.02 | 12.66 | -5.31 | -8.08 | 0.56 | 4.00 | 13.96 | 4.54 |
Bud Site | 1.61 | 2.00 | 1.57 | -0.24 | -1.75 | -9.74 | -1.41 | -8.20 | -3.34 | -4.81 | -8.00 | -6.42 | -9.71 | -3.23 | -3.67 |
Cell Periphery | 3.67 | 3.73 | 3.79 | 0.21 | 0.41 | -8.56 | -5.35 | -4.00 | 4.82 | 2.39 | -6.58 | -3.03 | -2.12 | 4.71 | 0.97 |
Cytoplasm | 2.59 | -0.19 | -2.13 | -7.33 | -6.06 | 8.52 | 19.85 | 12.12 | 4.86 | -10.09 | 9.05 | 21.57 | 13.86 | 4.61 | -8.71 |
Cytoplasmic Foci | -5.39 | 9.75 | 9.44 | 18.82 | -4.09 | 6.85 | 32.30 | 37.51 | 37.02 | 0.81 | 5.13 | 41.04 | 39.73 | 41.62 | -4.69 |
Eisosomes | 0.46 | 3.98 | 3.55 | 5.81 | -7.46 | -2.06 | 0.02 | 5.99 | 10.74 | 4.86 | -1.87 | 10.10 | 7.72 | 13.47 | -6.97 |
Endoplasmic Reticulum | 1.07 | -1.04 | -2.42 | -6.41 | -4.43 | -2.19 | -10.60 | -13.44 | -11.59 | 6.16 | -1.84 | -14.52 | -17.06 | -15.25 | -1.93 |
Endosome | -4.11 | 4.65 | 4.01 | 6.18 | -4.18 | -7.87 | 1.55 | 6.05 | 14.38 | 2.18 | -8.89 | 9.43 | 7.19 | 15.86 | -3.82 |
Golgi | -2.40 | 3.13 | 2.59 | 3.92 | -2.83 | -4.78 | 6.10 | 0.06 | 3.28 | -2.13 | -5.37 | 6.83 | 1.63 | 6.10 | -3.09 |
Lipid Particles | 2.35 | 4.68 | 4.51 | 5.28 | -3.76 | -0.13 | 6.80 | 10.28 | 13.46 | 5.03 | 0.30 | 11.09 | 10.90 | 13.94 | -1.54 |
Mitochondria | -2.17 | 2.94 | 3.09 | 3.26 | 1.90 | -9.33 | 7.51 | 1.60 | 9.81 | -3.72 | -9.50 | 5.74 | 4.35 | 11.53 | -1.39 |
Mitotic Spindle | 0.59 | -0.77 | -1.42 | -3.26 | -1.71 | -8.78 | -2.42 | -8.32 | -2.35 | -3.00 | -8.23 | -10.08 | -12.19 | -4.03 | -2.04 |
None | 2.65 | 0.83 | 0.43 | -6.53 | -1.12 | 0.12 | -2.30 | -1.42 | -1.45 | 1.27 | 0.69 | -2.53 | -2.24 | -2.77 | -0.49 |
Nuclear Periphery | 1.27 | -18.91 | -17.86 | -18.53 | -4.65 | -2.69 | -9.66 | -13.85 | -11.96 | 1.29 | -2.58 | -18.85 | -19.73 | -18.14 | -2.94 |
Nuclear Periphery Foci | 0.14 | -1.87 | -2.38 | -2.07 | -0.53 | 1.82 | -4.17 | -3.73 | -4.11 | 3.01 | 1.66 | -5.80 | -5.85 | -6.51 | 0.71 |
Nucleolus | 2.18 | -1.08 | 2.14 | -0.28 | 7.01 | -2.04 | -2.98 | 1.70 | 4.52 | 3.66 | -1.28 | -7.25 | 1.18 | 2.82 | 8.02 |
Nucleus | 5.16 | -12.24 | -4.47 | -20.38 | 23.20 | 0.42 | -24.40 | -15.85 | -16.16 | 14.83 | 1.92 | -52.56 | -22.61 | -24.89 | 29.57 |
Peroxisomes | -4.32 | 5.03 | 4.86 | 6.08 | -0.69 | -6.93 | 9.84 | 8.37 | 12.91 | -1.06 | -8.15 | 10.30 | 9.19 | 13.76 | -0.85 |
Vacuole | -1.40 | 2.13 | -9.81 | -9.12 | -24.96 | -11.70 | -7.58 | -23.11 | -17.60 | -9.18 | -11.67 | -1.78 | -32.14 | -23.34 | -31.54 |
Vacuole Periphery | 0.27 | 2.09 | 0.74 | 0.77 | -2.38 | -7.15 | 4.56 | -2.17 | 0.10 | -2.90 | -6.82 | 2.33 | -2.61 | 0.73 | -3.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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